Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha

Hyunah Kim; Eun Jung Thak; Ji Yoon Yeon; Min Jeong Sohn; Jin Ho Choo; Jeong-Yoon Kim; Hyun Ah Kang

doi:10.1007/s12275-018-7508-6

Articles

Page Path: HOME > J. Microbiol > Volume 56(1); 2018 > Article

Journal Article
Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha: Hyunah Kim ¹, Eun Jung Thak ¹, Ji Yoon Yeon ¹, Min Jeong Sohn ¹, Jin Ho Choo ¹, Jeong-Yoon Kim ², Hyun Ah Kang ¹; Journal of Microbiology 2018;56(1):72-82.
DOI: https://doi.org/10.1007/s12275-018-7508-6
Published online: January 4, 2018

¹Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea, ²Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Republic of Korea¹Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea, ²Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 34134, Republic of Korea

Corresponding author: Jeong-Yoon Kim , Tel: 82-2-820-5863,
Hyun Ah Kang , Tel: 82-2-820-5863,

Received: 22 November 2017 • Revised: 5 December 2017 • Accepted: 5 December 2017

47 Views
0 Download
8 Crossref
8 Scopus

Full Article

Abstract

Understanding the characteristics and regulation mechanisms of cell wall integrity (CWI) in yeast is important not only for basic research but also in biotechnological applications. We found significantly different CWIs in two representative strains of the thermotolerant methylotrophic yeast Hansenula polymorpha. Compared to the A16 strain (classified as Ogataea polymorpha), the DL1-L strain (classified as Ogataea parapolymorpha) has a thinner cell wall that was found to be more fragile following long-term cultivation and more sensitive to zymolyase. To gain a deeper insight into this difference, we compared the characteristics of the Mpk1pmediated CWI signaling pathway in the two strains. While a DL1-L mutant deficient in Mpk1p (mpk1Δ) showed severe growth retardation at both normal and high growth temperatures and in the presence of cell-wall disrupting agents, the A16 mpk1Δ mutant displayed only a mild defect in cell growth. Sorbitol effect on rescuing growth retardation was different in the two mpk1Δ strains, which could partly be ascribed to subtle differences in the activation of HOG pathway. Among the cell wall disruptors evaluated, only caffeine clearly increased phosphorylation of Mpk1p in DL1-L, but not in A16. A transcriptome analysis of the DL1-L strain revealed that caffeine significantly increased the expression of a subset of cell-wall related genes in an Mpk1p-dependent manner, but not the expected Rlm1-target genes. Taken together, our data support an essential role for Mpk1p in maintaining CWI in H. polymorpha, although the requirement for Mpk1p and its regulation under diverse stress conditions varies depending on the strain background.

Keywords: Hansenula polymorpha;cell wall integrity;Mpk1;Hog1;caffeine

Figure & Data

References

Citations

Citations to this article as recorded by

Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52
Moh Egy Rahman Firdaus, Apon Zaenal Mustopa, Nurlaili Ekawati, Sheila Chairunnisa, Rosyida Khusniatul Arifah, Ai Hertati, Shasmita Irawan, Anika Prastyowati, Arizah Kusumawati, Maritsa Nurfatwa
Journal of Genetic Engineering and Biotechnology.2023; 21(1): 68. CrossRef
Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
Tong-tong Zhu, Sheng-han Xiang, Lei Yang, Fang Tang, Wei Li, Ying-bao Liu, Wen-xiu Sun
European Journal of Plant Pathology.2022; 164(4): 525. CrossRef
The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts
Ricardo A. Ribeiro, Nuno Bourbon-Melo, Isabel Sá-Correia
Frontiers in Microbiology.2022;[Epub] CrossRef
The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C
Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Microbial Cell Factories.2021;[Epub] CrossRef
Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
Jin Ho Choo, Su-Bin Lee, Hye Yun Moon, Kun Hwa Lee, Su Jin Yoo, Keun Pil Kim, Hyun Ah Kang
Journal of Microbiology.2021; 59(2): 151. CrossRef
Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms
Woo-Hyun Chung
Journal of Microbiology and Biotechnology.2021; 31(2): 171. CrossRef
Short bZIP homologue of sulfur regulator Met4 from Ogataea parapolymorpha does not depend on DNA‐binding cofactors for activating genes in sulfur starvation
Su Jin Yoo, Min Jeong Sohn, Da Min Jeong, Hyun Ah Kang
Environmental Microbiology.2020; 22(1): 310. CrossRef
PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection
Liqun Jiang, Junjian Situ, Yi Zhen Deng, Lang Wan, Dandan Xu, Yubin Chen, Pinggen Xi, Zide Jiang
Frontiers in Microbiology.2018;[Epub] CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha

J. Microbiol. 2018;56(1):72-82. Published online January 4, 2018

DOI: https://doi.org/10.1007/s12275-018-7508-6

Related articles