- Direct interaction between the transcription factors CadC and OmpR involved in the acid stress response of Salmonella enterica
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Yong Heon Lee , Ji Hye Kim
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J. Microbiol. 2017;55(12):966-972. Published online December 7, 2017
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DOI: https://doi.org/10.1007/s12275-017-7410-7
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In Salmonella enterica serovar Typhimurium, the acid-sensing
regulator CadC activates transcription of the cadBA operon
which contributes to the acid tolerance response. The
DNA-binding response regulator OmpR in two-component
regulatory system with EnvZ binds to its own promoter for
autoinduction. We previously reported that CadC exerts a
negative influence on ompR transcription during acid adaptation.
However, its underlying mechanisms remain to be elucidated.
Here we show that the level of OmpR protein is gradually
reduced by a gradual increase in the CadC level using
an arabinose-inducible expression system, indicating there
exists a negative correlation between the expression levels of
two transcription factors. To explore the molecular basis for
OmpR repression by CadC, we performed in vitro binding assays
and determined that CadC directly interacts with OmpR.
We further show that inactivation of cadC inhibits transcription
of the fliC gene, which encodes the major flagellar subunit,
result
ing in impaired flagellar motility under acid-adaptation
conditions. Together, our findings suggest that CadC may
repress autoinduction of the OmpR response regulator through
their direct interaction.
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Citations
Citations to this article as recorded by  -
A current insight into
Salmonella’s
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Alkmini Gavriil, Ilias Giannenas, Panagiotis N. Skandamis
Critical Reviews in Food Science and Nutrition.2025; 65(20): 3835. CrossRef - Isolation and characterization of resistant variants of Salmonella Typhimurium after sequential exposure to plasma activated water (PAW)
Elisa Pagán, Foteini Pavli, Sarah Happiette, Daniel Berdejo, Ruben Gatt, Rafael Pagán, Vasilis Valdramidis, Diego García-Gonzalo
Innovative Food Science & Emerging Technologies.2024; 93: 103633. CrossRef - Molecular mechanism of proteolytic cleavage-dependent activation of CadC-mediated response to acid in E. coli
Min Chen, Ye Shang, Wenhao Cui, Xiaomeng Wang, Jiakun Zhu, Hongjie Dong, Hongwei Wang, Tiantian Su, Weiwei Wang, Kundi Zhang, Bingqing Li, Sujuan Xu, Wei Hu, Fengyu Zhang, Lichuan Gu
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Gut Microbes.2022;[Epub] CrossRef - Recent advances in understanding the effect of acid-adaptation on the cross-protection to food-related stress of common foodborne pathogens
Ricardo A. Wu, Hyun-Gyun Yuk, Donghong Liu, Tian Ding
Critical Reviews in Food Science and Nutrition.2022; 62(26): 7336. CrossRef - Metabolic adaptability shifts of cell membrane fatty acids of Komagataeibacter hansenii HDM1-3 improve acid stress resistance and survival in acidic environments
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- Innate signaling mechanisms controlling Mycobacterium chelonae-mediated CCL2 and CCL5 expression in macrophages
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Yi Sak Kim , Ji Hye Kim , Minjeong Woo , Tae-sung Kim Kim , Kyung Mok Sohn , Young-Ha Lee , Eun-Kyeong Jo , Jae-Min Yuk
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J. Microbiol. 2015;53(12):864-874. Published online December 2, 2015
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DOI: https://doi.org/10.1007/s12275-015-5348-1
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996
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Mycobacterium chelonae (Mch) is an atypical rapidly growing
mycobacterium (RGM) that belongs to the M. chelonae
complex, which can cause a variety of human infections.
During this type of mycobacterial infection, macrophagederived
chemokines play an important role in the mediation
of intracellular communication and immune surveillance
by which they orchestrate cellular immunity. However,
the intracellular signaling pathways involved in the macrophage-
induced chemokine production during Mch infections
remain unknown. Thus, the present study aimed to
determine the molecular mechanisms by which Mch activates
the gene expressions of chemokine (C-C motif) ligand
2 (CCL2) and CCL5 in murine bone marrow-derived macrophages
(BMDMs) and in vivo mouse model. Toll-like receptor
2 (TLR2)-deficient mice showed increased bacterial
burden in spleen and lung and decreased protein expression
of CCL2 and CCL5 in serum. Additionally, Mch infection
triggered the mRNA and protein expression of CCL2 and
CCL5 in BMDMs via TLR2 and myeloid differentiation
primary response gene 88 (MyD88) signaling and that it
rapidly activated nuclear factor (NF)-κB signaling, which is
required for the Mch-induced expressions of CCL2 and
CCL5 in BMDMs. Moreover, while the innate receptor
Dectin-1 was only partly involved in the Mch-induced expression
of the CCL2 and CCL5 chemokines in BMDMs,
the generation of intracellular reactive oxygen species (ROS)
was an important contributor to these processes. Taken together,
the present data indicate that the TLR2, MyD88,
and NF-κB pathways, Dectin-1 signaling, and intracellular
ROS generation contribute to the Mch-mediated expression
of chemokine genes in BMDMs.
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- The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores
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Yeo Jin Kim , Ji Yeon Kim , Ji Hye Kim , Seon Mee Yoon , Young-Bok Yoo , Se Won Yie
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J. Microbiol. 2008;46(1):95-99.
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DOI: https://doi.org/10.1007/s12275-007-0171-y
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788
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Abstract
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Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses.
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PLOS ONE.2020; 15(11): e0241749. CrossRef - Viruses infecting macrofungi
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