Journal of Microbiology

Jeong-Yoon Kim

11 Articles

The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans: Jong-Myeong Kim , Hye Yun Moon , Dong Wook Lee , Hyun Ah Kang , Jeong-Yoon Kim; J. Microbiol. 2021;59(11):1063-1063.; DOI: https://doi.org/10.1007/s12275-021-0326-2

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The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans: Jong-Myeong Kim , Hye Yun Moon , Dong Wook Lee , Hyun Ah Kang , Jeong-Yoon Kim; J. Microbiol. 2021;59(10):911-919. Published online September 7, 2021; DOI: https://doi.org/10.1007/s12275-021-1326-y

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Candida albicans is an opportunistic human pathogen that exists as yeast, hyphal or pseudohyphal forms depending on pH, nutrients, and temperature. The morphological transition from yeast to hyphae, which is required for the complete virulence of C. albicans, is controlled by many transcription factors that activate or repress hypha-specific genes. The C. albicans transcriptional factor Cas5, a key regulator of genes involved in cell wall integrity, affects the susceptibility of C. albicans to fluconazole, an inhibitor of ergosterol synthesis. In this study, we found that deletion of CAS5 in C. albicans decreased the expression levels of a set of ergosterol biosynthesis genes, such as ERG2, ERG3, ERG5, ERG6, ERG11, and ERG24,
result
ing in the accumulation of lanosterol and zymosterol, which are intermediate metabolites in the ergosterol biosynthesis pathway. Interestingly, it was observed that the cas5Δ/Δ mutant could not maintain the yeast form under non-hyphainducing conditions, while the CAS5-overexpressing cells could not form hyphae under hypha-inducing conditions. Consistent with these observations, the cas5Δ/Δ mutant highly expressed hypha-specific genes, ALS3, ECE1, and HWP1, under non-hypha-inducing conditions. In addition, CAS5 transcription was significantly downregulated immediately after hyphal initiation in the wild-type strain. Furthermore, the cas5Δ/Δ mutant reduced the transcription of NRG1, which encodes a major repressor of hyphal morphogenesis, while Cas5 overexpression increased the transcription of NRG1 under hyphainducing conditions. Collectively, this study suggests the potential role of Cas5 as a repressor of hypha-specific genes during yeast-form growth of C. albicans.

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Biocontrol effects and mechanisms of two Streptomyces strains against root rot of Codonopsis pilosula caused by Fusarium oxysporum
Wei Wang, Lulu Zhang, Zhanfeng Cao, Weijing Yang, Xingzheng Wang, Yufen Yang, Ziyi Hu, Shufeng Wei, Ning Yang
Physiological and Molecular Plant Pathology.2026; 145: 103378. CrossRef
Cas5 Regulates the Exposure of β-Glucan, the Cell Surface Hydrophobicity, and the Expression of Cell Wall Proteins to Remodel the Candida albicans Cell Wall and Participates in the Recruitment of Neutrophils
Qiyue Zhang, Guanglin Li, Yanmei Wang, Chen Yang, Wenhui Bai, Qingqing Li, Jiye Zhang, Peipei Zhang
Microorganisms.2025; 13(3): 683. CrossRef
The Role of Sfp1 in Candida albicans Cell Wall Maintenance
Che-Kang Chang, Min-Chi Yang, Hsueh-Fen Chen, Yi-Ling Liao, Chung-Yu Lan
Journal of Fungi.2022; 8(11): 1196. CrossRef

Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha: Hyunah Kim , Eun Jung Thak , Ji Yoon Yeon , Min Jeong Sohn , Jin Ho Choo , Jeong-Yoon Kim , Hyun Ah Kang; J. Microbiol. 2018;56(1):72-82. Published online January 4, 2018; DOI: https://doi.org/10.1007/s12275-018-7508-6

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Understanding the characteristics and regulation mechanisms of cell wall integrity (CWI) in yeast is important not only for basic research but also in biotechnological applications. We found significantly different CWIs in two representative strains of the thermotolerant methylotrophic yeast Hansenula polymorpha. Compared to the A16 strain (classified as Ogataea polymorpha), the DL1-L strain (classified as Ogataea parapolymorpha) has a thinner cell wall that was found to be more fragile following long-term cultivation and more sensitive to zymolyase. To gain a deeper insight into this difference, we compared the characteristics of the Mpk1pmediated CWI signaling pathway in the two strains. While a DL1-L mutant deficient in Mpk1p (mpk1Δ) showed severe growth retardation at both normal and high growth temperatures and in the presence of cell-wall disrupting agents, the A16 mpk1Δ mutant displayed only a mild defect in cell growth. Sorbitol effect on rescuing growth retardation was different in the two mpk1Δ strains, which could partly be ascribed to subtle differences in the activation of HOG pathway. Among the cell wall disruptors evaluated, only caffeine clearly increased phosphorylation of Mpk1p in DL1-L, but not in A16. A transcriptome analysis of the DL1-L strain revealed that caffeine significantly increased the expression of a subset of cell-wall related genes in an Mpk1p-dependent manner, but not the expected Rlm1-target genes. Taken together, our data support an essential role for Mpk1p in maintaining CWI in H. polymorpha, although the requirement for Mpk1p and its regulation under diverse stress conditions varies depending on the strain background.

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Phytosphingosine promotes intracellular microbial lipid accumulation and cell structure remodeling during adaptive evolution combined with cell fractionation in wild-type Yarrowia lipolytica DSM 3286
Shaokai Li, Lixia Pan, Bin Zhang, Jie Bao
Biochemical Engineering Journal.2026; 234: 110293. CrossRef
Thermotolerant yeasts promoting climate-resilient bioproduction
Roghayeh Shirvani, Maryam Babaei, Motahare Baladi, Matthias G Steiger, Mohammad Barshan-tashnizi
FEMS Yeast Research.2025;[Epub] CrossRef
Optimization, characterization, comparison of self-assembly VLP of capsid protein L1 in yeast and reverse vaccinology design against human papillomavirus type 52
Moh Egy Rahman Firdaus, Apon Zaenal Mustopa, Nurlaili Ekawati, Sheila Chairunnisa, Rosyida Khusniatul Arifah, Ai Hertati, Shasmita Irawan, Anika Prastyowati, Arizah Kusumawati, Maritsa Nurfatwa
Journal of Genetic Engineering and Biotechnology.2023; 21(1): 68. CrossRef
Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
Tong-tong Zhu, Sheng-han Xiang, Lei Yang, Fang Tang, Wei Li, Ying-bao Liu, Wen-xiu Sun
European Journal of Plant Pathology.2022; 164(4): 525. CrossRef
The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts
Ricardo A. Ribeiro, Nuno Bourbon-Melo, Isabel Sá-Correia
Frontiers in Microbiology.2022;[Epub] CrossRef
The damage and tolerance mechanisms of Phaffia rhodozyma mutant strain MK19 grown at 28 °C
Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Microbial Cell Factories.2021;[Epub] CrossRef
Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
Jin Ho Choo, Su-Bin Lee, Hye Yun Moon, Kun Hwa Lee, Su Jin Yoo, Keun Pil Kim, Hyun Ah Kang
Journal of Microbiology.2021; 59(2): 151. CrossRef
Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms
Woo-Hyun Chung
Journal of Microbiology and Biotechnology.2021; 31(2): 171. CrossRef
Short bZIP homologue of sulfur regulator Met4 from Ogataea parapolymorpha does not depend on DNA‐binding cofactors for activating genes in sulfur starvation
Su Jin Yoo, Min Jeong Sohn, Da Min Jeong, Hyun Ah Kang
Environmental Microbiology.2020; 22(1): 310. CrossRef
PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection
Liqun Jiang, Junjian Situ, Yi Zhen Deng, Lang Wan, Dandan Xu, Yubin Chen, Pinggen Xi, Zide Jiang
Frontiers in Microbiology.2018;[Epub] CrossRef

HST1 increases replicative lifespan of a sir2Δ mutant in the absence of PDE2 in Saccharomyces cerevisiae: Woo Kyu Kang , Mayur Devare , Jeong-Yoon Kim; J. Microbiol. 2017;55(2):123-129. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6535-z

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Abstract PDF

Silent information regulator 2 (Sir2), which is the founding member of the sirtuin family of proteins, is a pro-longevity factor for replicative lifespan (RLS) in Saccharomyces cerevisiae. Sir2 is required for transcriptional silencing at mating type loci, telomeres, and rDNA loci. Sir2 also represses transcription of highly expressed growth-related genes, such as PMA1 and some ribosomal protein genes. Although the Sir2 paralogues Hst1, Hst2, Hst3, and Hst4 occur in S. cerevisiae, none of them could replace the transcriptional regulation of PMA1 by Sir2 in the wild type. In this study, we demonstrate that Hst1, the closest Sir2 paralogue, deacetylates the acetylated lysine 16 of histone H4 (H4K16Ac) and represses PMA1 transcription in the sir2Δ pde2Δ mutant. We further show that Hst1 plays a role in extending the RLS of the sir2Δ pde2Δ mutant. Collectively, our results suggest that Hst1 can substitute for Sir2 by deacetylating H4K16Ac only in the sir2Δ pde2Δ.

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Selective production, relationship and controversy between Monascus pigments and citrinin
Xueling Qin, Bin Xie, Xuanli Zong, Xiang Yu, Yanli Feng
Food Bioscience.2023; 56: 103233. CrossRef
Gene repression in S. cerevisiae—looking beyond Sir-dependent gene silencing
Safia Mahabub Sauty, Kholoud Shaban, Krassimir Yankulov
Current Genetics.2021; 67(1): 3. CrossRef
Characterization of Resveratrol, Oxyresveratrol, Piceatannol and Roflumilast as Modulators of Phosphodiesterase Activity. Study of Yeast Lifespan
Adrián Matencio, Francisco García-Carmona, José Manuel López-Nicolás
Pharmaceuticals.2020; 13(9): 225. CrossRef
NAD+-dependent HDAC inhibitor stimulates Monascus pigment production but inhibit citrinin
Yan Hu, Youxiang Zhou, Zejing Mao, Huihui Li, Fusheng Chen, Yanchun Shao
AMB Express.2017;[Epub] CrossRef

Human fungal pathogens: Why should we learn?: Jeong-Yoon Kim; J. Microbiol. 2016;54(3):145-148.; DOI: https://doi.org/10.1007/s12275-016-0647-8

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Abstract PDF

Human fungal pathogens that cause invasive infections are hidden killers, taking lives of one and a half million people every year. However, research progress in this field has not been rapid enough to effectively prevent or treat life-threatening fungal diseases. To update recent research progress and promote more active research in the field of human fungal pathogens, eleven review articles concerning the virulence mechanisms and host interactions of four major human fungal pathogens–Candida albicans, Cryptococcus neoformans, Aspergillus fumigatus, and Histoplasma capsulatum–are presented in this special issue.

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Mahmoud M. El-Sonbaty, Mohamed A. Akl, Khalid M. El-Say, Alaa A. Kassem
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Guanylated Hyperbranched Polylysines with High In Vitro and In Vivo Antifungal Activity
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Tanaporn Wangsanut, Monsicha Pongpom
International Journal of Molecular Sciences.2022; 23(18): 10645. CrossRef
HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3
Han Wu, Xiaofan Yin, Xibao Zhao, Zherui Wu, Yue Xiao, Qianqian Di, Ping Sun, Haimei Tang, Jiazheng Quan, Weilin Chen
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Ernst H. Oliw
Fungal Genetics and Biology.2021; : 103603. CrossRef
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Ivan Ortega‐Blake, Mario Fernández‐Zertuche, Ignacio Regla, Walfred Sánchez‐Peña, Antonieta Gómez‐Solis, Paola Jaimes‐Chavez, Arturo Galván‐Hernández, Erika Tovar‐Garduño, Lourdes Rodríguez‐Fragoso
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Fengze Miao, Ying Li, Zongguang Tai, Yong Zhang, Yue Gao, Menghong Hu, Quangang Zhu
Macromolecular Bioscience.2021;[Epub] CrossRef
The transcription factor Cas5 suppresses hyphal morphogenesis during yeast-form growth in Candida albicans
Jong-Myeong Kim, Hye Yun Moon, Dong Wook Lee, Hyun Ah Kang, Jeong-Yoon Kim
Journal of Microbiology.2021; 59(10): 911. CrossRef
Antifungal activity of thiosemicarbazones, bis(thiosemicarbazones), and their metal complexes
Kritika Bajaj, Robert M. Buchanan, Craig A. Grapperhaus
Journal of Inorganic Biochemistry.2021; 225: 111620. CrossRef
Fatty acid dioxygenase-cytochrome P450 fusion enzymes of filamentous fungal pathogens
Ernst H. Oliw
Fungal Genetics and Biology.2021; 157: 103623. CrossRef
Membrane-Interacting Antifungal Peptides
Caroline Struyfs, Bruno P. A. Cammue, Karin Thevissen
Frontiers in Cell and Developmental Biology.2021;[Epub] CrossRef
Aspergillus fumigatus In-Host HOG Pathway Mutation for Cystic Fibrosis Lung Microenvironment Persistence
Brandon S. Ross, Lotus A. Lofgren, Alix Ashare, Jason E. Stajich, Robert A. Cramer, J. Andrew Alspaugh
mBio.2021;[Epub] CrossRef
Nanomaterial-Based Antifungal Therapies to Combat Fungal Diseases Aspergillosis, Coccidioidomycosis, Mucormycosis, and Candidiasis
Angel León-Buitimea, Javier A. Garza-Cervantes, Diana Y. Gallegos-Alvarado, Macario Osorio-Concepción, José Ruben Morones-Ramírez
Pathogens.2021; 10(10): 1303. CrossRef
Cathelicidin-inspired antimicrobial peptides as novel antifungal compounds
Martin van Eijk, Stephanie Boerefijn, Lida Cen, Marisela Rosa, Marnix J H Morren, Cornelis K van der Ent, Bart Kraak, Jan Dijksterhuis, Ivan D Valdes, Henk P Haagsman, Hans de Cock
Medical Mycology.2020; 58(8): 1073. CrossRef
Broad-Spectrum Antimicrobial and Antibiofilm Activity of a Natural Clay Mineral from British Columbia, Canada
Shekooh Behroozian, Sarah L. Svensson, Loretta Y. Li, Julian E. Davies, Pascale F. Cossart
mBio.2020;[Epub] CrossRef
Antimicrobial host defence peptides: functions and clinical potential
Neeloffer Mookherjee, Marilyn A. Anderson, Henk P. Haagsman, Donald J. Davidson
Nature Reviews Drug Discovery.2020; 19(5): 311. CrossRef
Alteration of Fermentative Metabolism Enhances Mucor circinelloides Virulence
Sharel P. Díaz-Pérez, J. Alberto Patiño-Medina, Marco I. Valle-Maldonado, Adolfo López-Torres, Irvin E. Jácome-Galarza, Verónica Anaya-Martínez, Verónica Gómez-Ruiz, Jesús Campos-García, Rosa E. Nuñez-Anita, Rafael Ortiz-Alvarado, Martha I. Ramírez-Díaz,
Infection and Immunity.2020;[Epub] CrossRef
Fungal evolution: major ecological adaptations and evolutionary transitions
Miguel A. Naranjo‐Ortiz, Toni Gabaldón
Biological Reviews.2019; 94(4): 1443. CrossRef
Acute Lung Injury and Repair Induced by Single Exposure of Aspergillus Fumigatus in Immunocompetent Mice
Nathália L S O Malacco, Jéssica A M Souza, Aline C Mendes, Milene A Rachid, Lucas R Kraemer, Matheus S Mattos, Graziele N Lima, Lirlândia P Sousa, Daniele G Souza, Vanessa Pinho, Mauro M Teixeira, Remo C Russo, Frederico M Soriani
Future Microbiology.2019; 14(17): 1511. CrossRef
Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth
Haomiao Ouyang, Ting Du, Hui Zhou, Iain B. H. Wilson, Jinghua Yang, Jean-Paul Latgé, Cheng Jin
Scientific Reports.2019;[Epub] CrossRef
Characterization of fungal biodiversity and communities associated with the reef macroalga Sargassum ilicifolium reveals fungal community differentiation according to geographic locality and algal structure
Benjamin J. Wainwright, Andrew G. Bauman, Geoffrey L. Zahn, Peter A. Todd, Danwei Huang
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Growth Phase-dependent Roles of Sir2 in Oxidative Stress Resistance and Chronological Lifespan in Yeast: Woo Kyu Kang , Yeong Hyeock Kim , Byoung-Soo Kim , Jeong-Yoon Kim; J. Microbiol. 2014;52(8):652-658. Published online July 5, 2014; DOI: https://doi.org/10.1007/s12275-014-4173-2

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Abstract PDF

Silent Information Regulator 2 (Sir2), a conserved NAD+- dependent histone deacetylase, has been implicated as one of the key factors in regulating stress response and longevity. Here, we report that the role of Sir2 in oxidative stress resistance and chronological lifespan is dependent on growth phase in yeast. In exponential phase, sir2Δ cells were more resistant to H2O2 stress and had a longer chronological lifespan than wild type. By contrast, in post-diauxic phase, sir2Δ cells were less resistant to H2O2 stress and had a shorter chronological lifespan than wild type cells. Similarly, the expression of antioxidant genes, which are essential to cope with oxidative stress, was regulated by Sir2 in a growth phasedependent manner. Collectively, our findings highlight the importance of the metabolic state of the cell in determining whether Sir2 can protect against or accelerate cellular aging of yeast.

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The intricate role of Sir2 in oxidative stress response during the post-diauxic phase in Saccharomyces cerevisiae
Yeong Hyeock Kim, Ji-In Ryu, Mayur Nimbadas Devare, Juhye Jung, Jeong-Yoon Kim
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Inactivation of MrSir2 in Monascus ruber Influenced the Developmental Process and the Production of Monascus Azaphilone Pigments
Jing Zhang, Yudan Yang, Zejing Mao, Qingqing Yan, Qi Chen, Ming Yi, Yanchun Shao
Applied Biochemistry and Biotechnology.2022; 194(12): 5702. CrossRef
Histone deacetylase SirE regulates development, DNA damage response and aflatoxin production in Aspergillus flavus
Meifang Wen, Huahui Lan, Ruilin Sun, Xuan Chen, Xin Zhang, Zhuo Zhu, Can Tan, Jun Yuan, Shihua Wang
Environmental Microbiology.2022; 24(11): 5596. CrossRef
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Samantha J Emery, Louise Baker, Brendan R E Ansell, Mehdi Mirzaei, Paul A Haynes, Malcom J McConville, Staffan G Svärd, Aaron R Jex
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Cell-Surface Expression of Aspergillus saitoi-Derived Functional α-1,2-Mannosidase on Yarrowia lipolytica for Glycan Remodeling: Hye Yun Moon , Trinh Luu Van , Seon Ah Cheon , Jinho Choo , Jeong-Yoon Kim , Hyun Ah Kang; J. Microbiol. 2013;51(4):506-514. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3344-x

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Abstract PDF

Expression of proteins on the surface of yeast has a wide range of applications, such as development of live vaccines, screening of antibody libraries, and use as whole-cell biocatalysts. The hemiascomycetes yeast Yarrowia lipolytica has been raised as a potential host for heterologous expression of recombinant proteins. In this study, we report the expression of Aspergillus saitoi α-1,2-mannosidase, encoded by the msdS gene, on the cell surface of Y. lipolytica. As the first step to achieve the secretory expression of msdS protein, four different signal sequences-derived from the endogenous Y. lipolytica Lip2 and Xpr2 prepro regions and the heterologous A. niger α-amylase and rice α-amylase signal sequences-were analyzed for their secretion efficiency. It was shown that the YlLip2 prepro sequence was most efficient in directing the secretory expression of msdS in fully N-glycosylated forms. The surface display of msdS was subsequently directed by fusing GPI anchoring motifs derived from Y. lipolytica cell wall proteins, YlCwp1p and YlYwp1p, respectively, to the C-terminus of the Lip2 prepro-msdS protein. The expression of actively functional msdS protein on the cell surface was confirmed by western blot, flow cytometry analysis, along with the α-1,2-mannosidase activity assay using intact Y. lipolytica cells as the enzyme source. Furthermore, the glycoengineered Y. lipolytica Δoch1Δmpo1 strains displaying α-1,2-mannosidase were able to convert Man8GlcNAc2 to Man5GlcNAc2 efficiently on their cell-wall mannoproteins, demonstrating its potential used for glycoengineering in vitro or in vivo.

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Engineering novel Yarrowia lipolytica whole-cell biocatalysts by cell surface display of the native Lip2 lipase for biodiesel production
Maria Orfanidou, Eleftheria Panagiotidou, Antonios M. Makris, Eleni Theodosiou
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Karla V. Teymennet-Ramírez, Fernando Martínez-Morales, María R. Trejo-Hernández
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Lili Song, Fei Wu, Congjun Li, Shicui Zhang
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Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin
Ewelina Celińska, Jean-Marc Nicaud, Wojciech Białas
Applied Microbiology and Biotechnology.2021; 105(3): 975. CrossRef
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Catherine Madzak
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Van-Trinh Luu, Hye Yun Moon, Jee Youn Hwang, Bo-Kyu Kang, Hyun Ah Kang
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Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
Microbial Cell Factories.2017;[Epub] CrossRef
Yarrowia lipolytica: recent achievements in heterologous protein expression and pathway engineering
Catherine Madzak
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Functional characterization of extracellular chitinase encoded by the YlCTS1 gene in a dimorphic yeast Yarrowia lipolytica
Jeong-Nam Park, Chang Pyo Han, Dong-Jik Lee, Seon Ah Cheon, Hyun Ah Kang
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Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha
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A Circularly Permuted β-Lactamase as a Novel Reporter for Evaluation of Protein Cyclization Efficiency: Jeong Seon Kwon , Jyotiranjan Bal , Hai Min Hwang , Jeong-Yoon Kim; J. Microbiol. 2008;46(4):456-461. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0106-2

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Split inteins have been used as a versatile tool in protein engineering to mediate efficient in vivo and in vitro trans-splicing of a protein. The trans-splicing ability of split inteins was also applied to the in vivo cyclization of a protein. However, cyclization efficiency is dependent upon the type of split inteins employed and the conditions under which cyclization occur. In this study, a novel reporter system that easily measures the cyclization efficiency of split inteins was developed. For this purpose TEM-1 β-lactamase was divided into two fragments (24~215 and 216~286 amino acids) and circularly permuted. The circularly permuted β-lactamase expressed in Escherichia coli showed little β-lactamase activity, most likely due to the structural modification of the protein. However, when the circularly permuted β-lactamase was cyclized by the Synechocystis sp. PCC6803 DnaB split mini-intein, β-lactamase activity both in vitro and in vivo was recovered. These results suggest that the novel reporter system can be exploited to develop new inteins with high efficiency of in vivo protein cyclization.

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Backbone cyclization of Salmonella typhimurium diaminopropionate ammonia-lyase to enhance the activity and stability
Xiaomei He, Tingting Lin, Yuying Xie, Jinjing Li, Yuanyuan Ge, Shuncheng Zhang, Jun Fan
Protein Expression and Purification.2024; 218: 106447. CrossRef
Off‐Pathway‐Sensitive Protein‐Splicing Screening Based on a Toxin/Antitoxin System
Hannes M. Beyer, Hideo Iwaï
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Circular Permutation of Bacillus circulans Xylanase: A Kinetic and Structural Study
Stephan Reitinger, Ying Yu, Jacqueline Wicki, Martin Ludwiczek, Igor D’Angelo, Simon Baturin, Mark Okon, Natalie C. J. Strynadka, Stefan Lutz, Stephen G. Withers, Lawrence P. McIntosh
Biochemistry.2010; 49(11): 2464. CrossRef

Role of CaBud6p in the Polarized Growth of Candida albicans: Yunkyoung Song , Jeong-Yoon Kim; J. Microbiol. 2006;44(3):311-319.; DOI: https://doi.org/2381 [pii]

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Abstract PDF: Bud6p is a component of a polarisome that controls cell polarity in Saccharomyces cerevisiae. In this study, we investigated the role of the Candida albicans Bud6 protein (CaBud6p) in cell polarity and hyphal development. CaBud6p, which consists of 703 amino acids, had 37% amino-acid sequence identity with the Bud6 protein of S. cerevisiae. The homozygous knock-out of CaBUD6 resulted in several abnormal phenotypes, such as a round and enlarged cells, widened bud necks, and a random budding pattern. In hypha-inducing media, the mutant cells had markedly swollen tips and a reduced ability to switch from yeast to hypha. In addition, a yeast two-hybrid analysis showed a physical interaction between CaBud6p and CaAct1p, which suggests that CaBud6p may be involved in actin cable organization, like Bud6p in S. cerevisiae. Taken together, these results indicate that CaBud6 plays an important role in the polarized growth of C. albicans.

Isolation and Characterization of Bud6p, an Actin Interacting Protein, from Yarrowia lipolytica: Yunkyoung Song , Seon Ah Cheon , So-Yeon Lee , Ji-Sook Hwang , Jeong-Yoon Kim; J. Microbiol. 2003;41(2):121-128.

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Abstract PDF: The identification of genes involved in true hypha formation is important in the study of mechanisms underlying the morphogenetic switch in yeast. We isolated a gene responsible for the morphogenetic switch in Yarrowia lipolytica, which forms true hyphae in response to serum or N-acetylglucosamine. The isolated gene, encoding 847 amino acids, had sequence identities of 27% and 25% with the Bud6 (Aip3) proteins of Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. Disruption of this gene, designated YlBUD6, in haploid and diploid strains significantly reduced the ability of Y. lipolytica to switch from the yeast form to the hyphal form in hypha-inducing media. It was also found that YlBud6 mutants were rounder than the wild type when grown in the yeast form. These results indicate that the YlBud6 protein is necessary for hyphal growth and cell polarity in both haploid and diploid Y. lipolytica cells.

Cloning and characterization of the multiprotein bridging factor 1 (YlMBF1) gene from the dimorphic yeast Yarrowia lipolytica: Janghwan Kim , Seon Ah Cheon , Yunkyoung Song , Jeong-Yoon Kim; J. Microbiol. 2002;40(2):173-177.

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Abstract PDF: In order to identify Yarrowia lipolytica genes induced by serum, cDNA representational difference analysis was performed using a PCR-select cDNA subtraction method. One of the genes cloned from the subtraction was a gene (YlMBF1) homologous to Saccharomyces cerevisiae MBF1 encoding the coactivator multiprotein bridging factor 1. Disruption of YlMBF1 revealed that the gene was not essential for viability, and the Ylmbf1[delta] strain did not show any distinct phenotypic change on solid serum medium. In liquid medium, however, a difference was found in the ability to maintain hyphae induced by serum. This result suggests that the YlMbf1 protein may mediate transcriptional activation of certain genes involved in the hypha formation of Y. lipolytica.

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