- DNA vaccine dual-expressing viral hemorrhagic septicemia virus glycoprotein and C-C motif chemokine ligand 19 induces the expression of immune-related genes in zebrafish (Danio rerio)
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Jin-Young Kim , Hyoung Jun Kim , Jeong Su Park , Se Ryun Kwon
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J. Microbiol. 2022;60(10):1032-1038. Published online August 1, 2022
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DOI: https://doi.org/10.1007/s12275-022-2231-8
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Glycoprotein (G protein)-based DNA vaccines are effective
in protecting aquaculture fish from rhabdoviruses but the degree
of immune response they elicit depends on plasmid concentration
and antigen cassette. Here, we developed a DNA
vaccine using the viral hemorrhagic septicemia virus G (VG)
gene and chemokine (C-C motif) ligand 19 (CCL19)a.2 regulated
by the CMV promoter as the molecular adjuvant. After
transfection of the prepared plasmid (pVG + CCL19) into epithelioma
papulosum cyprini cells, mRNA expression was confirmed
through quantitative real-time polymerase chain reaction.
The vaccine was intramuscularly injected into zebrafish
(Danio rerio), and 28 days after immunization, viral hemorrhagic
septicemia virus (105 TCID50/10 μl/fish) was intraperitoneally
injected. A survival rate of 68% was observed in the
pVG + CCL19 group but this was not significantly different
from the survival rate of fish treated with pVG alone, that is,
without the adjuvant. However, the expression of interferonand
cytokine-related genes in the spleen and kidney tissues
of zebrafish was significantly increased (p < 0.05) on days 1,
3, 7, and 14 after immunization. Thus, CCL19a.2 induced an
initial immune response as a molecular adjuvant, which may
provide initial protection against virus infection before vaccination-
induced antibody formation. This study provides insights
on the functions of CCL19a.2 adjuvant in DNA vaccines.
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Citations
Citations to this article as recorded by  - Interleukin-12 as an adjuvant enhances Th1-biased immune response in DNA vaccine against Edwardsiella tarda in flounder (Paralichthys olivaceus)
Xiaoyan Wu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Aquaculture.2026; 612: 743296. CrossRef - CXCL20a promotes the immune protection of an inactivated vaccine against grass carp reovirus infection in grass carp (Ctenopharyngodon idella)
Yizhong Lu, Shijie Wang, Yuezong Xu, Bo Tang, Guanyu Chen, Jianguo Su, Chunrong Yang
Fish & Shellfish Immunology.2026; 170: 111140. CrossRef - Comparative analysis of immunogenicity for viral hemorrhagic septicemia virus (VHSV) vaccines inactivated by different methods
Jin-Young Kim, Wi-Sik Kim, Su-Mi Shin, Taeho Kim, Sung-Ju Jung
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Aquaculture.2023; 576: 739882. CrossRef - Determining transcriptomic response of kidneys of olive flounder to viral hemorrhagic septicemia virus infection using next-generation sequencing
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- Development of a stringent ELISA protocol to evaluate anti-viral hemorrhagic septicemia virus-specific antibodies in olive flounder Paralichthys olivaceus with improved specificity
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Hyoung Jun Kim , Jeong Su Park , Se Ryun Kwon
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J. Microbiol. 2015;53(7):481-485. Published online June 27, 2015
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DOI: https://doi.org/10.1007/s12275-015-5101-9
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1,022
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Abstract
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Olive flounder were vaccinated with polyinosinic:polycytidylic
acid [Poly (I:C)] to prevent viral hemorrhagic septicemia
(VHS). Vaccine efficacy was verified by detection of anti-
VHS virus (VHSV) antibodies using enzyme-linked immunosorbent
assay (ELISA). In the study, ELISA absorbance values
of the negative control group [Poly (I:C)-MEM10] were saturated
when an ELISA protocol, that includes pretreatment
of the fish sera with 5% skim milk, was used. However,
the saturated OD values in the negative control did not correlate
with a specific immune response against VHSV, because
the group showed low survival rate (only 10%) following
the VHSV challenge. Also, OD values of Poly (I:C)-
VHSV group were high, and the group showed high survival
rate (97.5%) against VHSV challenge test. It was suggested
that the high OD values were possibly due to the
presence of anti-fetal bovine serum (FBS) cross-reactivity.
To compensate this, we subtracted the absorbance of infectious
hematopoietic necrosis (IHNV)-Ag plates from
those of the VHSV-Ag plates. However, the average value
for the Poly (I:C)-VHSV group (0.167) was lower than expected
even though high survival rate. We used an advanced
ELISA system to pre-treat fish sera with 5% skim milk and
two novirhabdoviruses as capture antigens as well as 50%
FBS. The corrected absorbance values for pre-treated fish
sera from the negative control Poly (I:C)-MEM10 and experimental
Poly (I:C)-VHSV groups averaged 0.033 and
0.579, respectively. The specific VHSV antibody response
of the vaccinated group was assessed using fish sera pretreated
with skim milk and FBS and by calculating the corrected
absorbance values from ELISA with two novirhabdovirus
capture antigens.
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Citations
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K. A. S. N. Shanaka, K. P. Madushani, Myoung‐Jin Kim, Sumi Jung, Jehee Lee
Reviews in Aquaculture.2025;[Epub] CrossRef - Assessment and Performance of Pooled Serum Samples for Monitoring Farm-Level Immunity in Tilapia Infected with Tilapia Lake Virus
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Jee Youn Hwang, Seongdo Lee, Thanthrige Thiunuwan Priyathilaka, Hyerim Yang, Hyukjae Kwon, Mun Gyeong Kwon, Seong Don Hwang, Myoung-Jin Kim, Jehee Lee
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Julio M. Coll
Frontiers in Immunology.2018;[Epub] CrossRef - Validation of a serum neutralization test for detection of antibodies specific to cyprinid herpesvirus 3 in infected common and koi carp (Cyprinus carpio)
J Cabon, L Louboutin, J Castric, S Bergmann, G Bovo, M Matras, O Haenen, N J Olesen, T Morin
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F. Torrent, A. Villena, P. A. Lee, W. Fuchs, S. M. Bergmann, J. M. Coll
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