Journal of Microbiology

Jae-Seoun Hur

7 Articles

Isolation and Characterization of a Reducing Polyketide Synthase Gene from the Lichen-Forming Fungus Usnea longissima: Yi Wang , Jung A Kim , Yong Hwa Cheong , Yogesh Joshi , Young Jin Koh , Jae-Seoun Hur; J. Microbiol. 2011;49(3):473-480. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0362-4

740 View
0 Download
9 Crossref

The reducing polyketide synthases found in filamentous fungi are involved in the biosynthesis of many drugs and toxins. Lichens produce bioactive polyketides, but the roles of reducing polyketide synthases in lichens remain to be clearly elucidated. In this study, a reducing polyketide synthase gene (UlPKS3) was isolated and characterized from a cultured mycobiont of Usnea longissima. Complete sequence information regarding UlPKS3 (6,519 bp) was obtained by screening a fosmid genomic library. A UlPKS3 sequence analysis suggested that it contains features of a reducing fungal type I polyketide synthase with β-ketoacyl synthase (KS), acyltransferase (AT), dehydratase (DH), enoyl reductase (ER), ketoacyl reducatse (KR), and acyl carrier protein (ACP) domains. This domain structure was similar to the structure of ccRads1, which is known to be involved in resorcylic acid lactone biosynthesis in Chaetomium chiversii. The results of phylogenetic analysis located UlPKS3 in the clade of reducing polyketide synthases. RT-PCR analysis results demonstrated that UlPKS3 had six intervening introns and that UlPKS3 expression was upregulated by glucose, sorbitol, inositol, and mannitol.

Citations

Citations to this article as recorded by

Genomic Features of Taiwanofungus gaoligongensis and the Transcriptional Regulation of Secondary Metabolite Biosynthesis
Yadong Zhang, Yi Wang, Xiaolong Yuan, Hongling Zhang, Yuan Zheng
Journal of Fungi.2024; 10(12): 826. CrossRef
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation
Jianhua Guo, Xiaoxu Zhao, Jie Shi
Biotechnology Progress.2024;[Epub] CrossRef
Discovery and excavation of lichen bioactive natural products
Meirong Ren, Shuhua Jiang, Yanyan Wang, Xinhua Pan, Feng Pan, Xinli Wei
Frontiers in Microbiology.2023;[Epub] CrossRef
Lichen Biosynthetic Gene Clusters. Part I. Genome Sequencing Reveals a Rich Biosynthetic Potential
Robert L. Bertrand, Mona Abdel-Hameed, John L. Sorensen
Journal of Natural Products.2018; 81(4): 723. CrossRef
Tissue-specific localization of polyketide synthase and other associated genes in the lichen, Cladonia rangiferina, using laser microdissection
Mostafa E. Elshobary, Michael G. Becker, Jenna L. Kalichuk, Ainsley C. Chan, Mark F. Belmonte, Michele D. Piercey-Normore
Phytochemistry.2018; 156: 142. CrossRef
A comprehensive catalogue of polyketide synthase gene clusters in lichenizing fungi
Robert L Bertrand, John L Sorensen
Journal of Industrial Microbiology and Biotechnology.2018; 45(12): 1067. CrossRef
In vitro risk assessment of usnic acid
Zühal Polat, Elanur Aydın, Hasan Türkez, Ali Aslan
Toxicology and Industrial Health.2016; 32(3): 468. CrossRef
Algal carbohydrates affect polyketide synthesis of the lichen-forming fungusCladonia rangiferina
Mostafa E. Elshobary, Mohamed E. Osman, Atef M. Abo-Shady, Emy Komatsu, Hélène Perreault, John Sorensen, Michele D. Piercey-Normore
Mycologia.2016; 108(4): 646. CrossRef
Genome characteristics reveal the impact of lichenization on lichen-forming fungus Endocarpon pusillum Hedwig (Verrucariales, Ascomycota)
Yan-Yan Wang, Bin Liu, Xin-Yu Zhang, Qi-Ming Zhou, Tao Zhang, Hui Li, Yu-Fei Yu, Xiao-Ling Zhang, Xi-Yan Hao, Meng Wang, Lei Wang, Jiang-Chun Wei
BMC Genomics.2014; 15(1): 34. CrossRef

Production of Anti-Helicobacter pylori Metabolite by the Lichen-Forming Fungus Nephromopsis pallescens: Heng Luo , Yoshikazu Yamamoto , Hae-Sook Jeon , Yan Peng Liu , Jae Sung Jung , Young Jin Koh , Jae-Seoun Hur; J. Microbiol. 2011;49(1):66-70. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0289-9

784 View
0 Download
13 Crossref

Abstract PDF

The present study was conducted to evaluate the antibacterial activity of lichen-forming fungi (LFF) against Helicobacter pylori, and to optimize the culture conditions of LFF for maximum production of natural antibiotics against H. pylori. To accomplish this, a screening assay was first conducted among 19 species of LFF. The extract of Nephromopsis pallescens (KOLRI-040516) exhibited the strongest anti-H. pylori activity. Bioautograghic TLC and HPLC analysis identified usnic acid as the main antibacterial substance produced by N. pallescens. The growth of N. pallescens and production of antibacterial substances produced by the fungus were then investigated under several culture conditions including the culture media, initial medium pHs, incubation temperatures, and the degree of aeration. The results indicated that culture in MY medium with an initial pH of 6.0, a temperature of 15°C and a low degree of aeration supported the largest usnic acid production of the fungus (16.4 μg usnic acid/g dry biomass). Especially, aeration was found to be an important factor that affect both growth and usnic acid production of N. pallescens.

Citations

Citations to this article as recorded by

Cercosporamide, a polyketide-derived fungal metabolite, serves as an antifungal agent against phytopathogenic fungi
Rundong Liu, Jaycee Augusto Paguirigan, Jae-Seoun Hur, Wonyong Kim
Mycoscience.2024; 65(1): 19. CrossRef
Faecal microbiota transplantation for eradicating Helicobacter pylori infection: clinical practice and theoretical postulation
Zhi-Ning Ye, Guy D Eslick, Shao-Gang Huang, Xing-Xiang He
eGastroenterology.2024; 2(4): e100099. CrossRef
Usnic acid: from an ancient lichen derivative to promising biological and nanotechnology applications
D. C. S. Macedo, F. J. F. Almeida, M. S. O. Wanderley, M. S. Ferraz, N. P. S. Santos, A. M. Q. López, N. S. Santos-Magalhães, M. C. B. Lira-Nogueira
Phytochemistry Reviews.2021; 20(3): 609. CrossRef
Bark extract influence on spore germination in corticolous lichen Xanthoria parietina in vitro
Hanne Marie Ellegård Larsen, Hanne Nina Rasmussen
Mycological Progress.2021; 20(3): 313. CrossRef
Helicobacter pylori infection and eradication: Exploring their impacts on the gastrointestinal microbiota
Zhi‐Hang Tao, Ji‐Xuan Han, Jing‐Yuan Fang
Helicobacter.2020;[Epub] CrossRef
Antimicrobial Activity of Divaricatic Acid Isolated from the Lichen Evernia mesomorpha against Methicillin-Resistant Staphylococcus aureus
Jong Min Oh, Yi Jeong Kim, Hyo-Seung Gang, Jin Han, Hyung-Ho Ha, Hoon Kim
Molecules.2018; 23(12): 3068. CrossRef
Usnic acid and its derivatives for pharmaceutical use: a patent review (2000–2017)
Olga A. Luzina, Nariman F. Salakhutdinov
Expert Opinion on Therapeutic Patents.2018; 28(6): 477. CrossRef
Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens
Yi Wang, Changan Geng, Xiaolong Yuan, Mei Hua, Fenghua Tian, Changtian Li, Marie-Joelle Virolle
PLOS ONE.2018; 13(7): e0199110. CrossRef
Biological activity of usnic acid and its derivatives: Part 1. Activity against unicellular organisms
O. A. Luzina, N. F. Salakhutdinov
Russian Journal of Bioorganic Chemistry.2016; 42(2): 115. CrossRef
Parmeliaceae family: phytochemistry, pharmacological potential and phylogenetic features
M. Pilar Gómez-Serranillos, Carlos Fernández-Moriano, Elena González-Burgos, Pradeep Kumar Divakar, Ana Crespo
RSC Adv..2014; 4(103): 59017. CrossRef
Biopharmaceutical potential of lichens
Vasudeo P. Zambare, Lew P. Christopher
Pharmaceutical Biology.2012; 50(6): 778. CrossRef
A new reducing polyketide synthase gene from the lichen-forming fungus Cladonia metacorallifera
Jung A Kim, Soon Gyu Hong, Yong Hwa Cheong, Young Jin Koh, Jae-Seoun Hur
Mycologia.2012; 104(2): 362. CrossRef
Recent literature on lichens—222
Sarah Z. Hodkinson, Brendan P. Hodkinson
The Bryologist.2011; 114(3): 653. CrossRef

Lichen Flora around the Korean Antarctic Scientific Station, King George Island, Antarctic: Ji Hee Kim , In-Young Ahn , Soon Gyu Hong , Mikhail Andreev , Kwang-Mi Lim , Mi Jin Oh , Young Jin Koh , Jae-Seoun Hur; J. Microbiol. 2006;44(5):480-491.; DOI: https://doi.org/2450 [pii]

583 View
0 Download

Abstract PDF: As part of the long-term monitoring projects on Antarctic terrestrial vegetation in relation to global climate change, a lichen floristical survey was conducted around the Korean Antarctic Station (King Sejong Station), which is located on Barton Peninsula, King George Island, in January and February of 2006. Two hundred and twenty-five lichen specimens were collected and sixty-two lichen species in 38 genera were identified by morphological characteristics, chemical constituents, TLC analysis and ITS nucleotide sequence analysis.

Highland Macrolichen Flora of Northwestern Yunnan, China: Jae-Seoun Hur , Li-Song Wang , Soon-Ok Oh , Gyoung Hee Kim , Kwang-Mi Lim , Jae-Sung Jung , Young Jin Koh; J. Microbiol. 2005;43(3):228-236.; DOI: https://doi.org/2222 [pii]

547 View
2 Download

Abstract PDF: Fifty-six species in 36 genera of macrolichens are reported from the Zhongdian area, northwest Yunnan, China during the lichenological expedition for highland macrolichen survey in June, 2004. More than 60% of these species have not been reported in South Korea. All of the 182 collected specimens are deposited in the Korean Lichen Research Institute (KoLRI) at Sunchon National University in Korea, and some of them are duplicated in the lichen herbarium, Crytogamic Herbarium, Kunming Institute of Botany, Academia Sinica (KUN-L) in China. This is the first report on the macrolichen flora in the visited areas.

Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants: Hyo Shim Han , Young Jin Koh , Jae-Seoun Hur , Jae Sung Jung; J. Microbiol. 2004;42(4):365-368.; DOI: https://doi.org/2096 [pii]

585 View
2 Download

Abstract PDF: The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA→CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.

Introduction of Saxicolous Lichens Distributed in Coastal Rocks of U-do Islet in Jeju, Korea: Hyung-Yeel Kahng , Byoung-Jun Yoon , Sung-Hyun Kim , Duck-Ja Shin , Jae-Seoun Hur , Hyun-Woo Kim , Eui-Sung Kang , Kye-Heon Oh , Young Jin Koh; J. Microbiol. 2004;42(4):292-298.; DOI: https://doi.org/2108 [pii]

549 View
0 Download

Abstract PDF: This study reports, for the first time, the ivestigation of the distribution of Korean saxicolous lichens in the coastal rocks of U-do islet, which is known as an unpolluted zone in Jeju. More than thirty lichens were obtained and investigated from the coastal rocks frequently contacted by seawater. A molecular analysis using PCR amplification of the rRNA ITS regions revealed the coastal rock lichens could be placed into 8 families and 14 genera, Ramalinaceae (Bacidia, Ramalina), Physciaceae (Buellia, Dirinaria, Phaeophyscia, Physcia, Pyxine), Lecanoraceae (Candelaria, Lecanora), Parmeliaceae (Xanthoparmelia), Graphidaceae (Graphis), Pertusariaceae (Pertusaria), Rhizocarpaceae (Rhizocarpon), and Teloschistaceae (Caloplaca), showing a diversity of lichens, with foliose (flat leaf-like), crustose (crust-like), and fruticose (miniature shrub-like) life forms might be distributed in the coastal rocks. These findings suggested the possibility that the lichens identified in the present work might be resistant to a salty environment.

Distribution of Lichen Flora on South Korea: Jae-Seoun Hur , Hiroshi Harada , Soon-Ok Oh , Kwang-Mi Lim , Eui-Sung Kang , Seung Mi Lee , Hyung-Yeel Kahng , Hyun-Woo Kim , Jae-Sung Jung , Young Jin Koh; J. Microbiol. 2004;42(2):163-167.

683 View
4 Download

Abstract PDF: After an overview on the temporary situation of the lichenology in South Korea, localities of 95 macrolichen taxa are reported for South Korea. In this revised lichen flora of South Korea, 16 species are apparently new to the territory. Voucher specimens have been deposited in the Korean Lichen Research Institute (KoLRI) at Sunchon National University in Korea, and duplicates have also been donated to the National History Museum and Institute, in Chiba, (CBM) Japan.

Author index