Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 49(1); 2011 > Article
Research Support, Non-U.S. Gov't
Production of Anti-Helicobacter pylori Metabolite by the Lichen-Forming Fungus Nephromopsis pallescens
Heng Luo 1, Yoshikazu Yamamoto 2, Hae-Sook Jeon 1, Yan Peng Liu 1, Jae Sung Jung 1, Young Jin Koh 1, Jae-Seoun Hur 1
Journal of Microbiology 2011;49(1):66-70.
DOI: https://doi.org/10.1007/s12275-011-0289-9
Published online: March 3, 2011
1Korean Lichen Research Institute, Sunchon National University, Sunchon 540-742, Republic of Korea, 2Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University, Akita 010-0195, Japan
Corresponding author:  Jae-Seoun Hur , Tel: +82-61-750-3383, 
Received: 2 August 2010   • Accepted: 3 November 2010
prev next
  • 211 Views
  • 0 Download
  • 13 Crossref
  • 18 Scopus
Full Article

Download PDF Download PDF

Abstract

The present study was conducted to evaluate the antibacterial activity of lichen-forming fungi (LFF) against Helicobacter pylori, and to optimize the culture conditions of LFF for maximum production of natural antibiotics against H. pylori. To accomplish this, a screening assay was first conducted among 19 species of LFF. The extract of Nephromopsis pallescens (KOLRI-040516) exhibited the strongest anti-H. pylori activity. Bioautograghic TLC and HPLC analysis identified usnic acid as the main antibacterial substance produced by N. pallescens. The growth of N. pallescens and production of antibacterial substances produced by the fungus were then investigated under several culture conditions including the culture media, initial medium pHs, incubation temperatures, and the degree of aeration. The results indicated that culture in MY medium with an initial pH of 6.0, a temperature of 15°C and a low degree of aeration supported the largest usnic acid production of the fungus (16.4 μg usnic acid/g dry biomass). Especially, aeration was found to be an important factor that affect both growth and usnic acid production of N. pallescens.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Production of Anti-Helicobacter pylori Metabolite by the Lichen-Forming Fungus Nephromopsis pallescens
    J. Microbiol. 2011;49(1):66-70.   Published online March 3, 2011
    DOI: https://doi.org/10.1007/s12275-011-0289-9
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP