- Production of Anti-Helicobacter pylori Metabolite by the Lichen-Forming Fungus Nephromopsis pallescens
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Heng Luo , Yoshikazu Yamamoto , Hae-Sook Jeon , Yan Peng Liu , Jae Sung Jung , Young Jin Koh , Jae-Seoun Hur
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J. Microbiol. 2011;49(1):66-70. Published online March 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0289-9
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The present study was conducted to evaluate the antibacterial activity of lichen-forming fungi (LFF) against Helicobacter pylori, and to optimize the culture conditions of LFF for maximum production of natural antibiotics against H. pylori. To accomplish this, a screening assay was first conducted among 19 species of
LFF. The extract of Nephromopsis pallescens (KOLRI-040516) exhibited the strongest anti-H. pylori activity. Bioautograghic TLC and HPLC analysis identified usnic acid as the main antibacterial substance produced by N. pallescens. The growth of N. pallescens and production of antibacterial substances produced by the
fungus were then investigated under several culture conditions including the culture media, initial medium pHs, incubation temperatures, and the degree of aeration. The results indicated that culture in MY medium with an initial pH of 6.0, a temperature of 15°C and a low degree of aeration supported the largest usnic acid production of the fungus (16.4 μg usnic acid/g dry biomass). Especially, aeration was found to be
an important factor that affect both growth and usnic acid production of N. pallescens.
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Citations
Citations to this article as recorded by  - Cercosporamide, a polyketide-derived fungal metabolite, serves as an antifungal agent against phytopathogenic fungi
Rundong Liu, Jaycee Augusto Paguirigan, Jae-Seoun Hur, Wonyong Kim
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Zhi-Ning Ye, Guy D Eslick, Shao-Gang Huang, Xing-Xiang He
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D. C. S. Macedo, F. J. F. Almeida, M. S. O. Wanderley, M. S. Ferraz, N. P. S. Santos, A. M. Q. López, N. S. Santos-Magalhães, M. C. B. Lira-Nogueira
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Yi Wang, Changan Geng, Xiaolong Yuan, Mei Hua, Fenghua Tian, Changtian Li, Marie-Joelle Virolle
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- Detection of Xanthomonas arboricola pv. pruni by PCR Using Primers Based on DNA Sequences Related to the hrp Genes
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So Yeon Park , Young Sun Lee , Young Jin Koh , Jae-Sun Hur , Jae Sung Jung
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J. Microbiol. 2010;48(5):554-558. Published online November 3, 2010
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DOI: https://doi.org/10.1007/s12275-010-0072-3
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947
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Efficient control of Xanthomonas arboricola pv. pruni, the causal agent of bacterial spot on stone fruit, requires a sensitive and reliable diagnostic tool. A PCR detection method that utilizes primers to target the hrp gene cluster region was developed in this study. The nucleotide sequence of the PCR product amplified with primers specific for the hrp region of the xanthomonads and genomic DNA of X. arboricola pv. pruni was determined, and the sequence was aligned with that of X. campestris pv. campestris, which was obtained from the GenBank database. On the basis of the sequence of the amplified hrp region, a PCR primer set of XapF/R specific to X. arboricola pv. pruni was designed. This primer set yielded a 243-bp product from the genomic DNA of X. aboricola pv. pruni strains, but no products from other 21 strains of Xanthomonas or from two epiphytic bacterial species. Southern blot hybridization with the probe derived from the PCR product with the primer set and X. aboricola pv. pruni DNA confirmed the PCR results. The Xap primer system was successfully applied to detect the pathogen from infected peach fruits. When it was applied in field samples, the primer set was proved as a reliable diagnostic tool for specific detection of X. aboricola pv. pruni from peach orchards.
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Citations
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Wenqing Lu, Wenxiao Du, Changlong Chen
International Journal of Molecular Sciences.2026; 27(6): 2639. CrossRef - Advancing Sustainable Management of Bacterial Spot of Peaches: Insights into Xanthomonas arboricola pv. pruni Pathogenicity and Control Strategies
Nanami Sakata, Yasuhiro Ishiga
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Weilan Li, Seung-Yeol Lee, Chang-Gi Back, Leonid N. Ten, Hee-Young Jung
The Plant Pathology Journal.2019; 35(6): 635. CrossRef - Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. pruni in symptomatic field samples
Pablo López-Soriano, Patricia Noguera, María Teresa Gorris, Rosa Puchades, Ángel Maquieira, Ester Marco-Noales, María M. López, Ulrich Melcher
PLOS ONE.2017; 12(4): e0176201. CrossRef - Pan-Genomic Analysis Permits Differentiation of Virulent and Non-virulent Strains of Xanthomonas arboricola That Cohabit Prunus spp. and Elucidate Bacterial Virulence Factors
Jerson Garita-Cambronero, Ana Palacio-Bielsa, María M. López, Jaime Cubero
Frontiers in Microbiology.2017;[Epub] CrossRef - Development of a nanostructured immunosensor for early and in situ detection of Xanthomonas arboricola in agricultural food production
Matías Regiart, Martin Rinaldi-Tosi, Pedro R. Aranda, Franco A. Bertolino, Jhonny Villarroel-Rocha, Karim Sapag, Germán A. Messina, Julio Raba, Martín A. Fernández-Baldo
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EPPO Bulletin.2015; 45(1): 41. CrossRef - The Best Spray Timing for the Control of the Bacterial Shot Hole with Bordeaux mixture (6-6) after Wintering in the Peach Orchard
San Yeong Kim, Won Heum Park, Hee Jung Son, Suk Hee Lee, Young Woon Song, So Deuk Park
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J.F. Pothier, M.C. Pagani, C. Pelludat, D.F. Ritchie, B. Duffy
Journal of Microbiological Methods.2011; 86(1): 16. CrossRef
- Aquimarina litoralis sp. nov., Isolated from a Coastal Seawater
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You-Sung Oh , Hyung-Yeel Kahng , Young Sun Lee , Byoung-Jun Yoon , Sang-Bin Lim , Jae Sung Jung , Duck-Chul Oh , Dong-Heon Lee
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J. Microbiol. 2010;48(4):433-437. Published online August 20, 2010
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DOI: https://doi.org/10.1007/s12275-010-0088-8
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A strictly aerobic, red-pigmented, non-motile, catalase- and oxidase-positive, Gram-staining-negative bacterium, designated strain CNURIC011T, was isolated from seawater off the coast of Jeju Island in Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNURIC011T belongs to the genus Aquimarina in the family Flavobacteriaceae. 16S rRNA gene sequence analysis revealed that the close relatives of the novel strain are Aquimarina latercula ATCC 23177T, Aquimarina marcrocephali JAMB N27T, Aquimarina intermedia KMM 6258T, Aquimarina muelleri KMM 6020T, and Aquimarina brevivitae SMK-19T, with sequence similarities of 97.6, 96.6, 96.0, 95.6, and 94.2%, respectively. DNA-DNA hybridization revealed that the level of relatedness between strain CNURIC011T and Aquimarina latercula ATCC 23177T (=KCTC 2912T) was 4.9%. The DNA G+C content was 35.8 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C15:0 (14.9%), C15:0 (13.9%), iso-C17:0 3-OH (12.6%), iso-C15:1 G (7.3%), and iso-C17:1 ω9c (7.2%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC011T represents a novel species within the genus Aquimarina, for which the name Aquimarina litoralis sp. nov. is proposed. The type strain is CNURIC011T (=KCTC 22614T =JCM 15974T).
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Citations
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Aquimarina amphilecti sp. nov., isolated from the sponge Amphilectus fucorum
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- Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants
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Hyo Shim Han , Young Jin Koh , Jae-Seoun Hur , Jae Sung Jung
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J. Microbiol. 2004;42(4):365-368.
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DOI: https://doi.org/2096 [pii]
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The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA→CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.
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