- Involvement of Curli Fimbriae in the Biofilm Formation of Enterobacter cloacae
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Sung-Min Kim , Hee-Woo Lee , Yeh-Wan Choi , Shuk-Ho Kim , Je-Chul Lee , Yoo-Chul Lee , Sung-Yong Seol , Dong-Taek Cho , Jungmin Kim
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J. Microbiol. 2012;50(1):175-178. Published online February 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-2044-2
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903
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Abstract
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In this study, we examined the biofilm forming ability, the
mRNA expression of curli genes and the morphologies of
curli fimbriae and biofilms in clinical isolates of Enterobacter
cloacae. The csgBA operon was found in 11 (78.6%) of the
14 isolates. The ability of E. cloacae isolates to form biofilms
was significantly correlated with the mRNA expression level
of the csgA and csgD genes. The curli protein fimbriae appeared
as tangled fibers and the curli-proficient strain formed
mature biofilms. Our data suggest that the expression of the
curli fimbriae play an important role in biofilm formation
in E. cloacae.
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- Proteomic Analysis of Acinetobacter baumannii in Biofilm and Planktonic Growth Mode
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Ji-Hyun Shin , Hee-Woo Lee , Sung-Min Kim , Jungmin Kim
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J. Microbiol. 2009;47(6):728-735. Published online February 4, 2010
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DOI: https://doi.org/10.1007/s12275-009-0158-y
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Abstract
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Recently, multidrug-resistant clinical isolates of Acinetobacter baumannii have been found to have a high capacity to form biofilm. It is well known that bacterial cells within biofilms are highly resistant to antibiotics, UV light, acid exposure, dehydration, and phagocytosis in comparison to their planktonic counterparts, which suggests that the cells in a biofilm have altered metabolic activity. To determine which proteins are up-regulated in A. baumannii biofilm cells, we performed a proteomic analysis. A clinical isolate of A. baumannii 1656-2, which was characterized to have a high biofilm forming ability, was cultivated under biofilm and planktonic conditions. Outer membrane enriched A. baumannii 1656-2 proteins were separated by two-dimensional (2-D) gel electrophoresis and the differentially expressed proteins were identified by MALDI-TOF mass spectrometry. The proteins up-regulated or expressed only in biofilm cells of A. baumannii are categorized as follows: (i) proteins processing environmental information such as the outer membrane receptor protein involved in mostly Fe transport, a sensor histidine kinase/response regulator, and diguanylate cyclase (PAS-GGEDF-EAL domain); (ii) proteins involved in metabolism such as NAD- linked malate dehydrogenase, nucleoside-diphosphate sugar epimerase, putative GalE, ProFAR isomerase, and N-acetylmuramoyl-L-alanine amidase; (iii) bacterial antibiotic resistance related proteins; and (iv) proteins related to gene repair such as exodeoxyribonuclease III and GidA. This proteomic analysis provides a fundamental platform for further studies to reveal the role of biofilm in the persistence and tolerance of A. baumannii.
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- Multidrug-Resistant Providencia Isolates Carrying blaPER-1, blaVIM-2, and armA
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Hee-Woo Lee , Hee-Young Kang , Kyeong-Seob Shin , Jungmin Kim
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J. Microbiol. 2007;45(3):272-274.
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DOI: https://doi.org/2531 [pii]
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Abstract
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During May to July 2004, three strains of Providencia spp. with multidrug-resistance (MDR) were isolated from urinary specimen of three patients hospitalized with a same hospital room. By PCR analysis, all three strains have been found to carry both VIM-2 type metallo-β-lactamase gene and PER-1 type extendedspectrum β-lactamase gene. One out of three strains carried additional resistance gene, armA, 16S rRNA methylase gene responsible for high level resistance to aminoglycosides. To our knowledge, this is the first report on the identification of Providencia spp. simultaneously carrying blaVIM-2, blaPER-1, and armA genes.
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