- [Protocol] Use of Cas9 Targeting and Red Recombination for Designer Phage Engineering
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Shin-Yae Choi , Danitza Xiomara Romero-Calle , Han-Gyu Cho , Hee-Won Bae , You-Hee Cho
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J. Microbiol. 2024;62(1):1-10. Published online February 1, 2024
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DOI: https://doi.org/10.1007/s12275-024-00107-2
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Abstract
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Bacteriophages (phages) are natural antibiotics and biological nanoparticles, whose application is significantly boosted by
recent advances of synthetic biology tools. Designer phages are synthetic phages created by genome engineering in a way
to increase the benefits or decrease the drawbacks of natural phages. Here we report the development of a straightforward
genome engineering method to efficiently obtain engineered phages in a model bacterial pathogen, Pseudomonas aeruginosa.
This was achieved by eliminating the wild type phages based on the Streptococcus pyogenes Cas9 (SpCas9) and facilitating
the recombinant generation based on the Red recombination system of the coliphage λ (λRed). The producer (PD) cells of
P. aeruginosa strain PAO1 was created by miniTn7-based chromosomal integration of the genes for SpCas9 and λRed under
an inducible promoter. To validate the efficiency of the recombinant generation, we created the fluorescent phages from a
temperate phage MP29. A plasmid bearing the single guide RNA (sgRNA) gene for selectively targeting the wild type gp35
gene and the editing template for tagging the Gp35 with superfolder green fluorescent protein (sfGFP) was introduced into
the PD cells by electroporation. We found that the targeting efficiency was affected by the position and number of sgRNA.
The fluorescent phage particles were efficiently recovered from the culture of the PD cells expressing dual sgRNA molecules.
This protocol can be used to create designer phages in P. aeruginosa for both application and research purposes.
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Citations
Citations to this article as recorded by 
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Pilin regions that select for the small RNA phages in
Pseudomonas aeruginosa
type IV pilus
Hee-Won Bae, Hyeong-Jun Ki, Shin-Yae Choi, You-Hee Cho, Kristin N. Parent Journal of Virology.2025;[Epub] CrossRef - Characteristics of bioaerosols under high-ozone periods, haze episodes, dust storms, and normal days in Xi’an, China
Yiming Yang, Liu Yang, Xiaoyan Hu, Zhenxing Shen Particuology.2024; 90: 140. CrossRef - Airborne desert dust and aeromicrobiology over the Turkish Mediterranean coastline
Dale W. Griffin, Nilgün Kubilay, Mustafa Koçak, Mike A. Gray, Timothy C. Borden, Eugene A. Shinn Atmospheric Environment.2007; 41(19): 4050. CrossRef
- REVIEW] Antibacterial strategies inspired by the oxidative stress and response networks
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So Youn Kim , Chanseop Park , Hye-Jeong Jang , Bi-o Kim , Hee-Won Bae , In-Young Chung , Eun Sook Kim , You-Hee Cho
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J. Microbiol. 2019;57(3):203-212. Published online February 26, 2019
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DOI: https://doi.org/10.1007/s12275-019-8711-9
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149
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Abstract
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Oxidative stress arises from an imbalance between the excessive
accumulation of reactive oxygen species (ROS) and
a cell’s capability to readily detoxify them. Although ROS are
spontaneously generated during the normal oxygen respiration
and metabolism, the ROS generation is usually augmented
by redox-cycling agents, membrane disrupters, and
bactericidal antibiotics, which contributes their antimicrobial
bioactivity. It is noted that all the bacteria deploy an arsenal
of inducible antioxidant defense systems to cope with the
devastating effect exerted by the oxidative stress: these systems
include the antioxidant effectors such as catalases and
the master regulators such as OxyR. The oxidative stress response
is not essential for normal growth, but critical to survive
the oxidative stress conditions that the bacterial pathogens
may encounter due to the host immune response and/or
the antibiotic treatment. Based on these, we here define the
ROS-inspired antibacterial strategies to enhance the oxidative
stress of ROS generation and/or to compromise the bacterial
response of ROS detoxification, by delineating the ROSgenerating
antimicrobials and the core concept of the bacterial
response against the oxidative stress.
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Citations
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V. Vanitha, S. Vijayakumar, S. Prabhu, M. Nilavukkarasi, V.N. Punitha, E. Vidhya, P.K. Praseetha Gene Reports.2021; 25: 101393. CrossRef - Qiangji Jianli Decoction Alleviates Hydrogen Peroxide‐Induced Mitochondrial Dysfunction via Regulating Mitochondrial Dynamics and Biogenesis in L6 Myoblasts
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B. Lee, J.S. Hwang, D.G. Lee Journal of Applied Microbiology.2020; 129(2): 287. CrossRef - Antibiotic resistance mitigation: the development of alternative general strategies
Siti Nurhanna Riduan, Arunmozhiarasi Armugam, Yugen Zhang Journal of Materials Chemistry B.2020; 8(30): 6317. CrossRef - Nanocopper-loaded Black phosphorus nanocomposites for efficient synergistic antibacterial application
Dandan Zhang, Hui Ming Liu, XiuLin Shu, Jin Feng, Ping Yang, Peng Dong, XiaoBao Xie, QingShan Shi Journal of Hazardous Materials.2020; 393: 122317. CrossRef - The insertion of functional groups in organic selenium compounds promote changes in mitochondrial parameters and raise the antibacterial activity
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Helmut Sies Antioxidants.2020; 9(9): 852. CrossRef - Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent
Ozgun O. Ozdemir, Ferda Soyer ACS Omega.2020; 5(32): 19938. CrossRef - Oxidative Stress Transcriptional Responses of Escherichia coli at GaN Interfaces
Sara Gleco, Theophraste Noussi, Akamu Jude, Pramod Reddy, Ronny Kirste, Ramón Collazo, Dennis LaJeunesse, Albena Ivanisevic ACS Applied Bio Materials.2020; 3(12): 9073. CrossRef - Oxidative Stress-Generating Antimicrobials, a Novel Strategy to Overcome Antibacterial Resistance
Álvaro Mourenza, José A. Gil, Luís M. Mateos, Michal Letek Antioxidants.2020; 9(5): 361. CrossRef - A Novel Screening Strategy Reveals ROS-Generating Antimicrobials That Act Synergistically against the Intracellular Veterinary Pathogen Rhodococcus equi
Álvaro Mourenza, José A. Gil, Luís M. Mateos, Michal Letek Antioxidants.2020; 9(2): 114. CrossRef - Copper/Carbon Hybrid Nanozyme: Tuning Catalytic Activity by the Copper State for Antibacterial Therapy
Juqun Xi, Gen Wei, Lanfang An, Zhuobin Xu, Zhilong Xu, Lei Fan, Lizeng Gao Nano Letters.2019; 19(11): 7645. CrossRef - Perspectives towards antibiotic resistance: from molecules to population
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- Superinfection Exclusion Reveals Heteroimmunity between Pseudomonas aeruginosa Temperate Phages
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In-Young Chung , Hee-Won Bae , Hye-Jung Jang , Bi-o Kim , You-Hee Cho
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J. Microbiol. 2014;52(6):515-520. Published online May 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4012-5
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Abstract
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Temperate siphophages (MP29, MP42, and MP48) were isolated from the culture supernatant of clinical Pseudomonas aeruginosa isolates. The complete nucleotide sequences and annotation of the phage genomes revealed the overall synteny
to the known temperate P. aeruginosa phages such as MP22, D3112, and DMS3. Genome-level sequence analysis showed the conservation of both ends of the linear genome and the divergence at the previously identified dissimilarity
regions (R1 to R9). Protein sequence alignment of the c repressor (ORF1) of each phage enabled us to divide the six phages into two groups: D3112 group (D3112, MP29, MP42, and MP48) and MP22 group (MP22 and DMS3). Superinfection
exclusion was observed between the phages belonging to the same group, which was mediated by the specific interaction between the c repressor and the cognate operator. Based on these, we suggest that the temperate siphophages prevalent in the clinical strains of P. aeruginosa represent at least two distinct heteroimmunity groups.
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Citations
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- Phage against the Machine: The SIE-ence of Superinfection Exclusion
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Gilles Vergnaud, Cédric Midoux, Yann Blouin, Maria Bourkaltseva, Victor Krylov, Christine Pourcel Viruses.2018; 10(5): 245. CrossRef - Evolutionary Ecology of Prokaryotic Immune Mechanisms
Stineke van Houte, Angus Buckling, Edze R. Westra Microbiology and Molecular Biology Reviews.2016; 80(3): 745. CrossRef - A phage protein that inhibits the bacterial ATPase required for type IV pilus assembly
In-Young Chung, Hye-Jeong Jang, Hee-Won Bae, You-Hee Cho Proceedings of the National Academy of Sciences.2014; 111(31): 11503. CrossRef
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