- Functional analysis of Mpk1-mediated cell wall integrity signaling pathway in the thermotolerant methylotrophic yeast Hansenula polymorpha
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Hyunah Kim , Eun Jung Thak , Ji Yoon Yeon , Min Jeong Sohn , Jin Ho Choo , Jeong-Yoon Kim , Hyun Ah Kang
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J. Microbiol. 2018;56(1):72-82. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7508-6
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Understanding the characteristics and regulation mechanisms
of cell wall integrity (CWI) in yeast is important not
only for basic research but also in biotechnological applications.
We found significantly different CWIs in two representative
strains of the thermotolerant methylotrophic yeast
Hansenula polymorpha. Compared to the A16 strain (classified
as Ogataea polymorpha), the DL1-L strain (classified as
Ogataea parapolymorpha) has a thinner cell wall that was
found to be more fragile following long-term cultivation and
more sensitive to zymolyase. To gain a deeper insight into this
difference, we compared the characteristics of the Mpk1pmediated
CWI signaling pathway in the two strains. While
a DL1-L mutant deficient in Mpk1p (mpk1Δ) showed severe
growth retardation at both normal and high growth temperatures
and in the presence of cell-wall disrupting agents, the
A16 mpk1Δ mutant displayed only a mild defect in cell growth.
Sorbitol effect on rescuing growth retardation was different
in the two mpk1Δ strains, which could partly be ascribed to
subtle differences in the activation of HOG pathway. Among
the cell wall disruptors evaluated, only caffeine clearly increased
phosphorylation of Mpk1p in DL1-L, but not in A16.
A transcriptome analysis of the DL1-L strain revealed that
caffeine significantly increased the expression of a subset of
cell-wall related genes in an Mpk1p-dependent manner, but
not the expected Rlm1-target genes. Taken together, our data
support an essential role for Mpk1p in maintaining CWI in
H. polymorpha, although the requirement for Mpk1p and
its regulation under diverse stress conditions varies depending
on the strain background.
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Citations
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Roghayeh Shirvani, Maryam Babaei, Motahare Baladi, Matthias G Steiger, Mohammad Barshan-tashnizi
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Journal of Genetic Engineering and Biotechnology.2023; 21(1): 68. CrossRef - Characterization, expression patterns and functional analysis of PcMPK12 gene in Phytophthora capsici
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European Journal of Plant Pathology.2022; 164(4): 525. CrossRef - The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts
Ricardo A. Ribeiro, Nuno Bourbon-Melo, Isabel Sá-Correia
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Li-Li Miao, Shuang Chi, Ting-Ting Hou, Zhi-Pei Liu, Ying Li
Microbial Cell Factories.2021;[Epub] CrossRef - Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha
Jin Ho Choo, Su-Bin Lee, Hye Yun Moon, Kun Hwa Lee, Su Jin Yoo, Keun Pil Kim, Hyun Ah Kang
Journal of Microbiology.2021; 59(2): 151. CrossRef - Pleiotropic Effects of Caffeine Leading to Chromosome Instability and Cytotoxicity in Eukaryotic Microorganisms
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Journal of Microbiology and Biotechnology.2021; 31(2): 171. CrossRef - Short bZIP homologue of sulfur regulator Met4 from Ogataea parapolymorpha does not depend on DNA‐binding cofactors for activating genes in sulfur starvation
Su Jin Yoo, Min Jeong Sohn, Da Min Jeong, Hyun Ah Kang
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Frontiers in Microbiology.2018;[Epub] CrossRef
- Structural analysis of N-/O-glycans assembled on proteins in yeasts
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Eun Jung Thak , Jungho Kim , Dong-Jik Lee , Jeong Yoon Kim , Hyun Ah Kang
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J. Microbiol. 2018;56(1):11-23. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7468-x
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906
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Protein glycosylation, the most universal and diverse posttranslational
modification, can affect protein secretion, stability,
and immunogenicity. The structures of glycans attached
to proteins are quite diverse among different organisms and
even within yeast species. In yeast, protein glycosylation plays
key roles in the quality control of secretory proteins, and particularly
in maintaining cell wall integrity. Moreover, in pathogenic
yeasts, glycans assembled on cell-surface glycoproteins
can mediate their interactions with host cells. Thus, a
comprehensive understanding of protein glycosylation in various
yeast species and defining glycan structure characteristics
can provide useful information for their biotechnological
and clinical implications. Yeast-specific glycans are a target
for glyco-engineering; implementing human-type glycosylation
pathways in yeast can aid the production of recombinant
glycoproteins with therapeutic potential. The virulenceassociated
glycans of pathogenic yeasts could be exploited
as novel targets for antifungal agents. Nowadays, several glycomics
techniques facilitate the generation of species- and
strain-specific glycome profiles and the delineation of modified
glycan structures in mutant and engineered yeast cells.
Here, we present the protocols employed in our laboratory
to investigate the N- and O-glycan chains released from purified
glycoproteins or cell wall mannoproteins in several
yeast species.
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PLOS Pathogens.2025; 21(7): e1013325. CrossRef - Evolutionary unique N-glycan-dependent protein quality control system plays pivotal roles in cellular fitness and extracellular vesicle transport in Cryptococcus neoformans
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Vaccines.2024; 12(12): 1344. CrossRef - Novel Botrytis cinerea Zn(II)2Cys6 Transcription Factor BcFtg1 Enhances the Virulence of the Gray Mold Fungus by Promoting Organic Acid Secretion and Carbon Source Utilization
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Journal of Agricultural and Food Chemistry.2024; 72(34): 18824. CrossRef - Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Tamara M. Khlebodarova, Natalia V. Bogacheva, Andrey V. Zadorozhny, Alla V. Bryanskaya, Asya R. Vasilieva, Danil O. Chesnokov, Elena I. Pavlova, Sergey E. Peltek
Microorganisms.2024; 12(2): 346. CrossRef - Proteomic Analysis of Cell Wall Proteins with Various Linkages in Fusarium graminearum
Heeji Moon, Kyunghun Min, Jessica Winarto, Soobin Shin, Hosung Jeon, Dae-Geun Song, Hokyoung Son
Journal of Agricultural and Food Chemistry.2024; 72(11): 6028. CrossRef - Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018
David J. Harvey
Mass Spectrometry Reviews.2023; 42(1): 227. CrossRef - Rna-Seq Based Transcriptomic Analysis of the Non-Conventional Yeast Spathaspora Passalidarum During Melle-Boinot Cell Recycle in Xylose-Glucose Mixtures
Thiago Neitzel, Cleilton Santos Lima, Eduardo Hafemann, Douglas Antonio Alvaredo Paixão, Joaquim Martins Junior, Gabriela Felix Persinoti, Leandro Vieira dos Santos, jaciane ienczak
SSRN Electronic Journal .2022;[Epub] CrossRef - RNA-seq based transcriptomic analysis of the non-conventional yeast Spathaspora passalidarum during Melle-boinot cell recycle in xylose-glucose mixtures
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Renewable Energy.2022; 201: 486. CrossRef - Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion
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Metabolic Engineering.2022; 74: 36. CrossRef -
Extension of
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-Linked Mannosylation in the Golgi Apparatus Is Critical for Cell Wall Integrity Signaling and Interaction with Host Cells in Cryptococcus neoformans Pathogenesis
Eun Jung Thak, Ye Ji Son, Dong-Jik Lee, Hyunah Kim, Jung Ho Kim, Su-Bin Lee, Yu-Byeong Jang, Yong-Sun Bahn, Connie B. Nichols, J. Andrew Alspaugh, Hyun Ah Kang, Michael Lorenz
mBio.2022;[Epub] CrossRef - Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast
Ji-Yeon Kim, Woo Hong Joo, Dong-Soo Shin, Yong-Ill Lee, Chin Fen Teo, Jae-Min Lim
Analytical Biochemistry.2021; 621: 114152. CrossRef - Yeast-produced RBD-based recombinant protein vaccines elicit broadly neutralizing antibodies and durable protective immunity against SARS-CoV-2 infection
Jinkai Zang, Yuanfei Zhu, Yu Zhou, Chenjian Gu, Yufang Yi, Shuxia Wang, Shiqi Xu, Gaowei Hu, Shujuan Du, Yannan Yin, Yalei Wang, Yong Yang, Xueyang Zhang, Haikun Wang, Feifei Yin, Chao Zhang, Qiang Deng, Youhua Xie, Zhong Huang
Cell Discovery.2021;[Epub] CrossRef - What makes Komagataella phaffii non-conventional?
Özge Ata, Burcu Gündüz Ergün, Patrick Fickers, Lina Heistinger, Diethard Mattanovich, Corinna Rebnegger, Brigitte Gasser
FEMS Yeast Research.2021;[Epub] CrossRef - Water Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In Vitro
Marta Calatayud, Rosa Aragao Börner, Jonas Ghyselinck, Lynn Verstrepen, Jelle De Medts, Pieter Van den Abbeele, Claire L. Boulangé, Sarah Priour, Massimo Marzorati, Sami Damak
Nutrients.2021; 13(11): 3897. CrossRef - Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans
Jae-Hyung Jin, Kyung-Tae Lee, Joohyeon Hong, Dongpil Lee, Eun-Ha Jang, Jin-Young Kim, Yeonseon Lee, Seung-Heon Lee, Yee-Seul So, Kwang-Woo Jung, Dong-Gi Lee, Eunji Jeong, Minjae Lee, Yu-Byeong Jang, Yeseul Choi, Myung Ha Lee, Ji-Seok Kim, Seong-Ryong Yu,
Nature Communications.2020;[Epub] CrossRef - NEGATIVE ION MASS SPECTROMETRY FOR THE ANALYSIS OF N‐LINKED GLYCANS
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Mass Spectrometry Reviews.2020; 39(5-6): 586. CrossRef - Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
Eun Jung Thak, Su Jin Yoo, Hye Yun Moon, Hyun Ah Kang
FEMS Yeast Research.2020;[Epub] CrossRef -
Core
N
-Glycan Structures Are Critical for the Pathogenicity of Cryptococcus neoformans by Modulating Host Cell Death
Eun Jung Thak, Su-Bin Lee, Shengjie Xu-Vanpala, Dong-Jik Lee, Seung-Yeon Chung, Yong-Sun Bahn, Doo-Byoung Oh, Mari L. Shinohara, Hyun Ah Kang, J. Andrew Alspaugh
mBio.2020;[Epub] CrossRef - Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases
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Journal of Separation Science.2019; 42(1): 130. CrossRef - Impact of ∼omics in the detection and validation of potential anti-infective drugs
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Current Opinion in Pharmacology.2019; 48: 1. CrossRef
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