Journal of Microbiology

Choon-Mee Kim

4 Articles

Cyclic AMP-Receptor Protein Activates Aerobactin Receptor IutA Expression in Vibrio vulnificus: Choon-Mee Kim , Seong-Jung Kim , Sung-Heui Shin; J. Microbiol. 2012;50(2):320-325. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-2056-y

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The ferrophilic bacterium Vibrio vulnificus can utilize the siderophore aerobactin of Escherichia coli for iron acquisition via its specific receptor IutA. This siderophore piracy by V. vulnificus may contribute to its survival and proliferation, especially in mixed bacterial environments. In this study, we examined the effects of glucose, cyclic AMP (cAMP), and cAMP-receptor protein (Crp) on iutA expression in V. vulnificus. Glucose dose-dependently repressed iutA expression. A mutation in cya encoding adenylate cyclase required for cAMP synthesis severely repressed iutA expression, and this change was recovered by in trans complementing cya or the addition of exogenous cAMP. Furthermore, a mutation in crp encoding Crp severely repressed iutA expression, and this change was recovered by complementing crp. Accordingly, glucose deprivation under iron-limited conditions is an environmental signal for iutA expression, and Crp functions as an activator that regulates iutA expression in response to glucose availability.

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Aerobactin: A Multifaceted Siderophore
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Indian Journal of Microbiology.2026; 66(4): 843. CrossRef
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Bharath Reddy Boya, Jin‐Hyung Lee, Jintae Lee
Microbial Biotechnology.2025;[Epub] CrossRef
Interspecific cross-talk: The catalyst driving microbial biosynthesis of secondary metabolites
Guihong Yu, Xiaoxuan Ge, Wanting Li, Linwei Ji, Song Yang
Biotechnology Advances.2024; 76: 108420. CrossRef
Impact of substrate on opto-thermal response of thin metallic targets under irradiation with femtosecond laser pulses
G. D. Tsibidis, E. Stratakis
Journal of Central South University.2022; 29(10): 3410. CrossRef
Regulation of iron-uptake systems in Vibrio vulnificus, a ferrophilic bacterium
Sung-Heui Shin
Medical Biological Science and Engineering.2021; 4(2): 69. CrossRef
Characteristics of the iron uptake-related process of a pathogenic Vibrio splendidus strain associated with massive mortalities of the sea cucumber Apostichopus japonicus
Tongxiang Song, Huijie Liu, Tengteng Lv, Xuelin Zhao, Yina Shao, Qingxi Han, Chenghua Li, Weiwei Zhang
Journal of Invertebrate Pathology.2018; 155: 25. CrossRef
Stress responses of Acinetobacter strain Y during phenol degradation
Johnson Lin
Archives of Microbiology.2017; 199(2): 365. CrossRef
Vibrio Iron Transport: Evolutionary Adaptation to Life in Multiple Environments
Shelley M. Payne, Alexandra R. Mey, Elizabeth E. Wyckoff
Microbiology and Molecular Biology Reviews.2016; 80(1): 69. CrossRef
Temperature Change Induces the Expression of vuuA Encoding Vulnibactin Receptor and crp Encoding Cyclic AMP Receptor Protein in Vibrio vulnificus
Choon-Mee Kim, Young-Joon Ahn, Seong-Jung Kim, Dae-Heung Yoon, Sung-Heui Shin
Current Microbiology.2016; 73(1): 54. CrossRef
Cyclic AMP and Cyclic AMP-Receptor Protein are Required for Optimal Capsular Polysaccharide Expression
Hyeon-Kyu Cho, Sung-Heui Shin
Journal of Bacteriology and Virology.2015; 45(1): 26. CrossRef
Enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels
Mauricio Latorre, Jessica Galloway-Peña, Jung Hyeob Roh, Marko Budinich, Angélica Reyes-Jara, Barbara E. Murray, Alejandro Maass, Mauricio González
Metallomics.2014; 6(3): 572. CrossRef
Multiple High-affinity Iron-uptake Systems ofVibrio vulnificus
Sung-Heui Shin
Journal of Bacteriology and Virology.2013; 43(3): 168. CrossRef
Cyclic AMP and Cyclic AMP-receptor Protein Modulate the Autoinducer-2-Mediated Quorum Sensing System in Vibrio vulnificus
Sun-Pyo Kim, Choon-Mee Kim, Sung-Heui Shin
Current Microbiology.2012; 65(6): 701. CrossRef
Coordinate Regulation ofVibrio vulnificusHeme Receptor HupA Expression by Cyclic AMP-receptor Protein and Ferric Uptake Regulator
Sun-Pyo Kim, Gang-Wook Lee, Choon-Mee Kim, Sung-Heui Shin
Journal of Bacteriology and Virology.2012; 42(4): 294. CrossRef

Two Forms of Vibrio vulnificus Metalloprotease VvpE are Secreted via the Type II General Secretion System: Jong Park , So-Yeon Ryu , Choon-Mee Kim , Sung-Heui Shin; J. Microbiol. 2008;46(3):338-343. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-008-0058-6

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Abstract PDF

Vibrio vulnificus has been known to secrete one form of metalloprotease VvpE (45 kDa) that is cleaved to 34 kDa-VvpE and 11 kDa-C-terminal propeptide via extracellular autoproteolysis. However, we found that extracellular secretion of both the 34 and 45 kDa forms of VvpE began in the early growth phase; moreover, 34 kDa-VvpE existed as the major form in V. vulnificus cell lysates and culture supernatants. In addition, extracellular secretion of both 34 and 45 kDa-VvpE was blocked by mutation of the pilD gene, which encodes for the type IV leader peptidase/N-methyltransferase of the type II general secretion system, and the blocked VvpE secretion was recovered by in trans-complementation of the wild-type pilD gene. These results indicate that 34 kDa-VvpE is the major form secreted along with 45 kDa-VvpE from the early growth phase via the PilD-mediated type II general secretion system.

Citations

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Structure and assembly of pilotin-dependent and -independent secretins of the type II secretion system
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PLOS Pathogens.2019; 15(5): e1007731. CrossRef
Bacterial calpains and the evolution of the calpain (C2) family of peptidases
Neil D. Rawlings
Biology Direct.2015;[Epub] CrossRef
Vibrio vulnificus quorum-sensing system operates in cirrhotic ascites, a human ex vivo experimental system
Choon-Mee Kim, Sung-Heui Shin
Annals of Microbiology.2013; 63(1): 403. CrossRef
SmcR, the Quorum-sensing Master Regulator, Is Partially Involved in Temperature/Salinity-mediated Changes in MetalloproteasevvpEExpression inVibrio vulnificus
Choon-Mee Kim, Sung-Heui Shin
Journal of Bacteriology and Virology.2012; 42(1): 29. CrossRef
Change ofVibrio vulnificusMetalloprotease VvpE Production by Temperature and Salinity
Choon-Mee Kim, Sung-Heui Shin
Journal of Bacteriology and Virology.2011; 41(3): 147. CrossRef
Functional Characterization of EpsC, a Component of the Type II Secretion System, in the Pathogenicity of Vibrio vulnificus
Won Hwang, Na Yeon Lee, Juri Kim, Mi-Ae Lee, Kun-Soo Kim, Kyu-Ho Lee, Soon-Jung Park, S. R. Blanke
Infection and Immunity.2011; 79(10): 4068. CrossRef
Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire
Eidy de O Santos, Nelson Alves, Graciela M Dias, Ana Maria Mazotto, Alane Vermelho, Gary J Vora, Bryan Wilson, Victor H Beltran, David G Bourne, Frédérique Le Roux, Fabiano L Thompson
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Proteases Produced by Vibrios
SUMIO SHINODA, SHIN-ICHI MIYOSHI
Biocontrol Science.2011; 16(1): 1. CrossRef
A recombinant metalloprotease antigen ofVibrio vulnificuselicits protective antibodies in a murine model
Y.-C. Chen, C.-C. Chang, S.-Y. Chang, J.-H. Su
Letters in Applied Microbiology.2010; 50(2): 168. CrossRef
Current status and future prospects in a pathogenic study of Vibrio vulnificus
Takashige KASHIMOTO
Nippon Saikingaku Zasshi.2010; 65(3): 369. CrossRef

Vibrio vulnificus Metalloprotease VvpE has no Direct Effect on Iron-uptake from Human Hemoglobin: Hui-Yu Sun , Song-Iy Han , Mi-Hwa Choi , Seong-Jung Kim , Choon-Mee Kim , Sung-Heui Shin; J. Microbiol. 2006;44(5):537-547.; DOI: https://doi.org/2443 [pii]

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Abstract PDF: This study was designed to determine whether or not Vibrio vulnificus metalloprotease VvpE can promote iron uptake via the proteolytic cleavage of human hemoglobin. We found that V. vulnificus utilized hemoglobin as an iron source more efficiently via the vulnibactin-mediated iron-uptake system than via the HupA-mediated iron-uptake system and, of the proteases produced by V. vulnificus, VvpE was found to be the only protease capable of destroying hemoglobin. However, VvpE expression, on both the transcriptional and protein levels, was suppressed in iron-limited media. However, vvpE transcription, but not extracellular VvpE production, was reactivated by the addition of hemoglobin or inorganic iron into iron-limited media. Moreover, vvpE transcription began only in the late growth phase when V. vulnificus had already consumed most of the iron for growth. In addition, neither vvpE mutation nor in trans vvpE complementation affected the ability of V. vulnificus to acquire iron or to grow in iron-limited media or in cirrhotic ascites containing hemoglobin. Hemoglobin added into iron-limited media was not destroyed, but gradually formed an insoluble aggregate during culture; this aggregation of hemoglobin occurred regardless of vvpE mutation or complementation. These results indicate that VvpE is not required for efficient iron uptake from hemoglobin. On the contrary, hemoglobin or iron is required for efficient vvpE transcription. In addition, a discrepancy exists between vvpE transcription and extracellular VvpE production in iron-limited media containing inorganic iron or hemoglobin, which suggests that additional unknown posttranscriptional events may be involved in the extracellular production of VvpE.

Swarming Differentiation of Vibrio vulnificus Downregulates the Expression of the vvhBA Hemolysin Gene via the LuxS Quorum-Sensing System: Moon-Young Kim , Ra-Young Park , Mi-Hwa Choi , Hui-Yu Sun , Choon-Mee Kim , Soo-Young Kim , Joon-Haeng Rhee , Sung-Heui Shin; J. Microbiol. 2006;44(2):226-232.; DOI: https://doi.org/2361 [pii]

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Abstract PDF: Swarming has proven to be a good in vitro model for bacterial surface adherence and colonization, and the swarming differentiation of a bacterium has been shown to be coupled with changes in the expression of virulence factors associated with its invasiveness, particularly in the early stages of infection. In this study, we attempted to determine whether the expression of vvhA, which encodes for hemolysin/cytolysin (VvhA), is either upregulated or downregulated during the swarming differentiation of V. vulnificus. The insertional inactivation of vvhA itself exerted no detectable effect on the expression of V. vulnificus swarming motility. However, in our lacZ-fused vvhA transcriptional reporter assay, vvhA expression decreased in swarming V. vulnificus as compared to non-swarming or planktonic V. vulnificus. The reduced expression of vvhA in swarming V. vulnificus increased as a result of the deletional inactivation of luxS, a gene associated with quorum sensing. These results show that vvhA expression in swarming V. vulnificus is downregulated via the activity of the LuxS quorum-sensing system, suggesting that VvhA performs no essential role in the invasiveness of V. vulnificus via the adherence to and colonization on the body surfaces required in the early stages of the infection. However, VvhA may play a significant role in the pathophysiological deterioration occurring after swarming V. vulnificus is differentiated into planktonic V. vulnificus.

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