- Short-chain fatty acids inhibit the biofilm formation of Streptococcus gordonii through negative regulation of competence-stimulating peptide signaling pathway
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Taehwan Park , Jintaek Im , A Reum Kim , Dongwook Lee , Sungho Jeong , Cheol-Heui Yun , Seung Hyun Han
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J. Microbiol. 2021;59(12):1142-1149. Published online December 4, 2021
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DOI: https://doi.org/10.1007/s12275-021-1576-8
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 Abstract
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Streptococcus gordonii, a Gram-positive commensal bacterium,
is an opportunistic pathogen closely related to initiation
and progression of various oral diseases, such as periodontitis
and dental caries. Its biofilm formation is linked
with the development of such diseases by enhanced resistance
against antimicrobial treatment or host immunity. In the
present study, we investigated the effect of short-chain fatty
acids (SCFAs) on the biofilm formation of S. gordonii. SCFAs,
including sodium acetate (NaA), sodium propionate (NaP),
and sodium butyrate (NaB), showed an effective inhibitory
activity on the biofilm formation of S. gordonii without reduction
in bacterial growth. SCFAs suppressed S. gordonii
biofilm formation at early time points whereas SCFAs did
not affect its preformed biofilm. A quorum-sensing system
mediated by competence-stimulating peptide (CSP) is known
to regulate biofilm formation of streptococci. Interestingly,
SCFAs substantially decreased mRNA expression of comD
and comE, which are CSP-sensor and its response regulator
responsible for CSP pathway, respectively. Although S. gordonii
biofilm formation was enhanced by exogenous synthetic
CSP treatment, such effect was not observed in the
presence of SCFAs. Collectively, these results suggest that
SCFAs have an anti-biofilm activity on S. gordonii through
inhibiting comD and comE expression which results in negative
regulation of CSP quorum-sensing system. SCFAs could
be an effective anti-biofilm agent against S. gordonii for the
prevention of oral diseases.
-
Citations
Citations to this article as recorded by  - Potential effects of prebiotic fibers on dental caries: a systematic review
Constanza E. Fernández, Catalina Maturana‐Valenzuela, Nicol Rojas‐Castillo, Bob Rosier
Journal of the Science of Food and Agriculture.2025;[Epub] CrossRef - Serotype-Dependent Inhibition of Streptococcus pneumoniae Growth by Short-Chain Fatty Acids
Suwon Lim, Dongwook Lee, Sungho Jeong, Jeong Woo Park, Jintaek Im, Bokeum Choi, Donghyun Gwak, Cheol-Heui Yun, Ho Seong Seo, Seung Hyun Han
Journal of Microbiology and Biotechnology.2024; 34(1): 47. CrossRef - Comprehensive Multi-Omic Evaluation of the Microbiota and Metabolites in the Colons of Diverse Swine Breeds
Yanbin Zhu, Guangming Sun, Yangji Cidan, Bin Shi, Zhankun Tan, Jian Zhang, Wangdui Basang
Animals.2024; 14(8): 1221. CrossRef - Recent progress in understanding the role of bacterial extracellular DNA: focus on dental biofilm
Fengxue Geng, Junchao Liu, Jinwen Liu, Ze Lu, Yaping Pan
Critical Reviews in Microbiology.2024; : 1. CrossRef - Effects of Epigallocatechin gallate on Biofilm adherence and Glycolytic pH in Streptococcus gordonii
Prawati Nuraini, Dimas Prasetianto Wicaksono, Ardianti Maartrina Dewi, Adinda Ayu Fitriana, Sili Han
Research Journal of Pharmacy and Technology.2024; : 4711. CrossRef - Oral Pathogens and Their Antibiotics from Marine Organisms: A Systematic Review of New Drugs for Novel Drug Targets
Sehyeok Im, Jun Hyuck Lee, Youn-Soo Shim
Journal of Dental Hygiene Science.2024; 24(2): 84. CrossRef - Effects of the gut microbiota and its metabolite short-chain fatty acids on endometriosis
Menghe Liu, Ru Peng, Chunfang Tian, Jianping Shi, Jiannan Ma, Ruiwen Shi, Xiao Qi, Rongwei Zhao, Haibin Guan
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - Butyrate potentiates Enterococcus faecalis lipoteichoic acid-induced inflammasome activation via histone deacetylase inhibition
Ok-Jin Park, Ye-Eun Ha, Ju-Ri Sim, Dongwook Lee, Eun-Hye Lee, Sun-Young Kim, Cheol-Heui Yun, Seung Hyun Han
Cell Death Discovery.2023;[Epub] CrossRef - Gut microbiota short-chain fatty acids and their impact on the host thyroid function and diseases
María José Mendoza-León, Ashutosh K. Mangalam, Alejandro Regaldiz, Enrique González-Madrid, Ma. Andreina Rangel-Ramírez, Oscar Álvarez-Mardonez, Omar P. Vallejos, Constanza Méndez, Susan M. Bueno, Felipe Melo-González, Yorley Duarte, Ma. Cecilia Opazo, Al
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Yumeng Wang, Shixi Xu, Qiurong He, Kun Sun, Xiaowan Wang, Xiaorui Zhang, Yuqing Li, Jumei Zeng
Frontiers in Microbiology.2023;[Epub] CrossRef - Listening to enteric bacteria from the perspective of antibiotic alternatives in animal husbandry
Leli Wang, Yiru Zhang, Juan Xu, Qingqing Shi, Yao Peng, Cimin Long, Lan Li, Yulong Yin
The Innovation Life.2023; 1(2): 100022. CrossRef - The Complicated Relationship of Short-Chain Fatty Acids and Oral Microbiome: A Narrative Review
Georgy E. Leonov, Yurgita R. Varaeva, Elena N. Livantsova, Antonina V. Starodubova
Biomedicines.2023; 11(10): 2749. CrossRef - Social networking at the microbiome-host interface
Richard J. Lamont, George Hajishengallis, Hyun Koo, Anthony R. Richardson
Infection and Immunity.2023;[Epub] CrossRef - Making Sense of Quorum Sensing at the Intestinal Mucosal Interface
Friederike Uhlig, Niall P. Hyland
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Kirsi Savijoki, Paola San-Martin-Galindo, Katriina Pitkänen, Minnamari Edelmann, Annika Sillanpää, Cim van der Velde, Ilkka Miettinen, Jayendra Z. Patel, Jari Yli-Kauhaluoma, Mataleena Parikka, Adyary Fallarero, Pekka Varmanen
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Alessandro Polizzi, Martina Donzella, Giada Nicolosi, Simona Santonocito, Paolo Pesce, Gaetano Isola
Pharmaceutics.2022; 14(12): 2740. CrossRef
- Lactobacillus plantarum lipoteichoic acid disrupts mature Enterococcus faecalis biofilm
-
A Reum Kim , Minji Kang , Yeon-Jee Yoo , Cheol-Heui Yun , Hiran Perinpanayagam , Kee-Yeon Kum , Seung Hyun Han
-
J. Microbiol. 2020;58(4):314-319. Published online January 28, 2020
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DOI: https://doi.org/10.1007/s12275-020-9518-4
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73
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20
Web of Science
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19
Crossref
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Abstract
-
Apical periodontitis is caused by biofilm-mediated root canal
infection. Early phase oral bacterial biofilms are inhibited by
Lactobacillus plantarum lipoteichoic acid (Lp.LTA). However,
mature biofilms that develop over 3 weeks are more resistant
to traditional endodontic medicaments. Therefore, this study
examined the effectiveness of Lp.LTA on disrupting mature
Enterococcus faecalis biofilms, and on enhancing the effects
of endodontic medicaments. LTA was purified from L. plantarum
through butanol extraction followed by hydrophobic
and ion-exchange chromatography. E. faecalis biofilms were
formed over 3 weeks on glass bottom dishes and in dentin
blocks obtained from human single-rooted premolars. These
mature biofilms were treated with or without Lp.LTA for 1 h,
followed by additional treatment with either chlorhexidine
digluconate (CHX), calcium hydroxide (CH), or triple antibiotics
for 24 h. Biofilms on glass were live/dead stained and
quantified by ZEN through confocal laser microscopy. Biofilms
in dentin were fixed, sputter coated and analyzed by
ImageJ with scanning electron microscopy. Preformed E. faecalis
mature biofilms on the culture dishes were dose-dependently
disrupted by Lp.LTA. Lp.LTA potentiated the effects
of CHX or CH on the disruption of mature biofilm. Interestingly,
CHX-induced disruption of preformed E. faecalis
mature biofilms was synergistically enhanced only when pretreated
with Lp.LTA. Furthermore, in the dentin block model,
Lp.LTA alone reduced E. faecalis mature biofilm and
pre-treatment with Lp.LTA promoted the anti-biofilm activity
of CHX. Lp.LTA could be an anti-biofilm or supplementary
agent that can be effective for E. faecalis-biofilminduced
diseases.
-
Citations
Citations to this article as recorded by - A Systematic Review of the Comparative Efficacy of Lactobacillus Probiotics and Sodium Hypochlorite as Intracanal Irrigants Against Enterococcus faecalis
Mrinalini Mrinalini, Alpa Gupta, Dax Abraham, Arun Kumar Duraisamy, Rajat Sharma
Cureus.2024;[Epub] CrossRef -
The role of
Lactobacillus plantarum
in oral health: a review of current studies
Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai
Journal of Oral Microbiology.2024;[Epub] CrossRef - Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation
Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han
Journal of Microbiology.2024; 62(10): 897. CrossRef - Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT
Chengran Guan, Feng Li, Peng Yu, Xuan Chen, Yongqi Yin, Dawei Chen, Ruixia Gu, Chenchen Zhang, Bo Pang
Foods.2024; 13(17): 2706. CrossRef - Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
Prerna Saini, Repally Ayyanna, Rishi Kumar, Sayan Kumar Bhowmick, Vinay Bhaskar, Bappaditya Dey
Frontiers in Microbiology.2024;[Epub] CrossRef - Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis
Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2024; 62(8): 683. CrossRef - Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
Shymaa Shaaban, Salma Genena, Alaaeldin Elraggal, Gamal M. Hamad, Marwa A. Meheissen, Sybel Moussa
BMC Oral Health.2023;[Epub] CrossRef - Antioxidant Effects and Probiotic Properties of Latilactobacillus sakei MS103 Isolated from Sweet Pickled Garlic
Heng Li, Changlin Chen, Yuanxin Li, Zhengqiang Li, Chen Li, Chang Luan
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Giusy Rita Maria La Rosa, Eugenio Pedullà
Australian Endodontic Journal.2023; 49(S1): 528. CrossRef - Antibacterial efficacy of antibiotic pastes versus calcium hydroxide intracanal dressing: A systematic review and meta-analysis of ex vivo studies
Mohammadreza Vatankhah, Kamyar Khosravi, Nazanin Zargar, Armin Shirvani, MohammadHossein Nekoofar, Omid Dianat
Journal of Conservative Dentistry.2022; 25(5): 463. CrossRef - Plantaricin LD1 purified from Lactobacillus plantarum LD1 inhibits biofilm formation of Enterococcus faecalis ATCC 29212 in tooth model
M.K. Yadav, P. Yadav, M. Dhiman, S. Tewari, S.K. Tiwari
Letters in Applied Microbiology.2022; 75(3): 623. CrossRef - The Products of Probiotic Bacteria Effectively Treat Persistent Enterococcus faecalis Biofilms
Shatha Safadi, Harsh Maan, Ilana Kolodkin-Gal, Igor Tsesis, Eyal Rosen
Pharmaceutics.2022; 14(4): 751. CrossRef - Regenerative Endodontics and Minimally Invasive Dentistry: Intertwining Paths Crossing Over Into Clinical Translation
Hisham Elnawam, Menatallah Abdelmougod, Ahmed Mobarak, Mai Hussein, Hamdy Aboualmakarem, Michael Girgis, Rania El Backly
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Can Probiotics Emerge as Effective Therapeutic Agents in Apical Periodontitis? A Review
Gaurav Kumar, Sanjay Tewari, John Tagg, Michael Leonidas Chikindas, Igor V Popov, Santosh Kumar Tiwari
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Karen D. Zeise, Robert J. Woods, Gary B. Huffnagle
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M. Bertolini, R. Vazquez Munoz, L. Archambault, S. Shah, J. G. S. Souza, R. C. Costa, A. Thompson, Y. Zhou, T. Sobue, A. Dongari-Bagtzoglou, Deborah A. Hogan
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Vinoo Subramaniam Ramachandran, Mensudar Rathakrishnan, Malathy Balaraman Ravindrran, Alargarsamy Venkatesh, Vidhya Shankari Shanmugasundaram, Karpagavinayagam Kumaraguru
Journal of Pure and Applied Microbiology.2021; 15(2): 534. CrossRef - Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Dongwook Lee, Jintaek Im, Dong Hyun Park, Sungho Jeong, Miri Park, Seokmin Yoon, Jaewoong Park, Seung Hyun Han
Frontiers in Microbiology.2021;[Epub] CrossRef - Streptococcus gordonii: Pathogenesis and Host Response to Its Cell Wall Components
Ok-Jin Park, Yeongkag Kwon, Chaeyeon Park, Yoon Ju So, Tae Hwan Park, Sungho Jeong, Jintaek Im, Cheol-Heui Yun, Seung Hyun Han
Microorganisms.2020; 8(12): 1852. CrossRef
- Propionate, together with triple antibiotics, inhibits the growth of Enterococci
-
Soyoung Jeong , Yunjae Lee , Cheol-Heui Yun , Ok-Jin Park , Seung Hyun Han
-
J. Microbiol. 2019;57(11):1019-1024. Published online October 28, 2019
-
DOI: https://doi.org/10.1007/s12275-019-9434-7
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73
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15
Web of Science
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16
Crossref
-
Abstract
-
Enterococci are Gram-positive facultative anaerobic bacteria
that colonize the oral cavity and gastrointestinal tract. Enterococcal
infections, mainly caused by Enterococcus faecalis
and Enterococcus faecium, include apical periodontitis, endocarditis,
and bloodstream infections. Recently, vancomycinresistant
Enterococci are considered major pathogens that
are common but difficult to treat, especially in nosocomial
settings. Moreover, E. faecalis is closely associated with recurrent
endodontic infections and failed endodontic treatment.
In this study, we investigated the effects of short-chain
fatty acids (SCFAs), acetate, propionate, and butyrate, which
are metabolites fermented by gut microbiota, on the growth
of Enterococci. Enterococci were cultured in the presence
or absence of acetate, propionate, or butyrate, and the optical
density at 600 nm was measured to determine bacterial
growth. The minimum inhibitory concentration/minimum
bactericidal concentration test was conducted. Bacteria were
treated with a SCFA, together with clinically used endodontic
treatment methods such as triple antibiotics (metronidazole,
minocycline, and ciprofloxacin) and chlorhexidine gluconate
(CHX) to determine the effects of combination treatment.
Of the SCFAs, propionate had a bacteriostatic effect, inhibiting
the growth of E. faecalis in a dose-dependent manner and
also that of clinical strains of E. faecalis isolated from dental
plaques. Meanwhile, acetate and butyrate had minimal effects
on E. faecalis growth. Moreover, propionate inhibited the
growth of other Enterococci including E. faecium. In addition,
combination treatment of propionate and triple antibiotics
led to further growth inhibition, whereas no cooperative
effect was observed at propionate plus CHX. These results
indicate that propionate attenuates the growth of Enterococci,
suggesting propionate as a potential agent to control
Enterococcal infections, especially when combined with triple
antibiotics.
-
Citations
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Heekuk Park, Elissa Lynch, Alice Tillman, Kristen Lewis, Zhezhen Jin, Anne-Catrin Uhlemann, Julian A. Abrams, Daniel E. Freedberg
Critical Care.2025;[Epub] CrossRef - Repurposing astemizole to kill multidrug-resistant bacteria isolated in general surgery
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Microbial Pathogenesis.2025; 200: 107369. CrossRef - Serotype-Dependent Inhibition of Streptococcus pneumoniae Growth by Short-Chain Fatty Acids
Suwon Lim, Dongwook Lee, Sungho Jeong, Jeong Woo Park, Jintaek Im, Bokeum Choi, Donghyun Gwak, Cheol-Heui Yun, Ho Seong Seo, Seung Hyun Han
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Jia-Ying Yan, Tsung-Han Lin, Yu-Tang Jong, Jun-Wei Hsueh, Sze-Hsien Wu, Hsiu-Jung Lo, Yee-Chun Chen, Chien-Hsiung Pan
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Paul B. Stege, Dirkjan Schokker, Frank Harders, Soumya K. Kar, Norbert Stockhofe, Vera Perricone, Johanna M. J. Rebel, Ingrid C. de Jong, Alex Bossers
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Biomedicine & Pharmacotherapy.2022; 145: 112352. CrossRef - RNA-Seq-based transcriptome analysis of methicillin-resistant Staphylococcus aureus growth inhibition by propionate
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Frontiers in Microbiology.2022;[Epub] CrossRef - Propionate Attenuates Growth of Oral Streptococci through Enhancing Methionine Biosynthesis
Taehwan Park, Jintaek Im, A Reum Kim, Dongwook Lee, Sungho Jeong, Cheol-Heui Yun, Seung Hyun Han
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Tyler Alexander Cookson
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- Lipoteichoic acids of lactobacilli inhibit Enterococcus faecalis biofilm formation and disrupt the preformed biofilm
-
Solmin Jung , Ok-Jin Park , A Reum Kim , Ki Bum Ahn , Dongwook Lee , Kee-Yeon Kum , Cheol-Heui Yun , Seung Hyun Han
-
J. Microbiol. 2019;57(4):310-315. Published online January 22, 2019
-
DOI: https://doi.org/10.1007/s12275-019-8538-4
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99
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52
Web of Science
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49
Crossref
-
Abstract
-
Enterococcus faecalis, a Gram-positive bacterium commonly
isolated in patients with refractory apical periodontitis, invades
dentin tubules easily and forms biofilms. Bacteria in biofilms,
which contribute to recurrent and/or chronic inflammatory
diseases, are more resistant to antimicrobial agents
than planktonic cells and easily avoid phagocytosis. Although
Lactobacillus plantarum lipoteichoic acid (Lp.LTA) is associated
with biofilm formation, the effect of Lp.LTA on biofilm
formation by E. faecalis is not clearly understood. In this
study, we investigated whether Lp.LTA inhibits E. faecalis
biofilm formation. The degree of biofilm formation was determined
by using crystal violet assay and LIVE/DEAD bacteria
staining. The quantification of bacterial growth was determined
by measuring the optical density at 600 nm with a
spectrophotometer. Formation of biofilms on human dentin
slices was observed under a scanning electron microscope.
E. faecalis biofilm formation was reduced by Lp.LTA treatment
in a dose-dependent manner. Lp.LTA inhibited biofilm
development of E. faecalis at the early stage without affecting
bacterial growth. LTA from other Lactobacillus species
such as Lactobacillus acidophilus, Lactobacillus casei, or
Lactobacillus rhamnosus GG also inhibited E. faecalis biofilm
formation. In particular, among LTAs from various lactobacilli,
Lp.LTA showed the highest inhibitory effect on biofilms
formed by E. faecalis. Interestingly, LTAs from lactobacilli
could remove the biofilm preformed by E. faecalis.
These inhibitory effects were also observed on the surface of human dentin slices. In conclusion, Lactobacillus species LTA
inhibits biofilm formation caused by E. faecalis and it could
be used as an anti-biofilm agent for prevention or treatment
against E. faecalis-associated diseases.
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Citations
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Xiangdi Lou, Qiang Zhou, Qiyue Jiang, Liping Lin, Wenwu Zhu, Xiaoyu Mei, Jianhua Xiong, Yanyan Gao
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Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han
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Poultry Science.2024; 103(12): 104419. CrossRef -
The role of
Lactobacillus plantarum
in oral health: a review of current studies
Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai
Journal of Oral Microbiology.2024;[Epub] CrossRef - Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
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and Helicobacter pylori
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Hidden Agenda of
Enterococcus Faecalis
Lifestyle Transition: Planktonic To Sessile State
Parvathy Venkateswaran, Priya M Lakshmanan, Sudhiksha Muthukrishnan, Hema Bhagavathi, Sahana Vasudevan, Prasanna Neelakantan, Adline P Solomon
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Guzel ISAEVA, Regina ISAEVA
Minerva Gastroenterology.2022;[Epub] CrossRef - Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms
Jyoti Vishwakarma, Bhumika Waghela, Berness Falcao, Sirisha L. Vavilala
Applied Biochemistry and Biotechnology.2022; 194(2): 671. CrossRef - Phage therapy for refractory periapical periodontitis caused by Enterococcus faecalis in vitro and in vivo
Yingying Xiang, Chunlan Ma, Shuang Yin, Fei Song, Kunhao Qin, Yafang Ding, Xianghong Yang, Pengfei Song, Xiuling Ji, Yunlin Wei
Applied Microbiology and Biotechnology.2022; 106(5-6): 2121. CrossRef - Gram Positive Bacterial Lipoteichoic Acid Role in a Root Canal Infection – A Literature Review
Vinoo Subramaniam Ramachandran, Mensudar Rathakrishnan, Malathy Balaraman Ravindrran, Alargarsamy Venkatesh, Vidhya Shankari Shanmugasundaram, Karpagavinayagam Kumaraguru
Journal of Pure and Applied Microbiology.2021; 15(2): 534. CrossRef - In Vitro Effects of Lactobacillus plantarum LN66 and Antibiotics Used Alone or in Combination on Helicobacter pylori Mature Biofilm
Jianfu Ji, Hong Yang
Microorganisms.2021; 9(2): 424. CrossRef - Effects of Rose Bengal‐ and Methylene Blue‐Mediated Potassium Iodide‐Potentiated Photodynamic Therapy on Enterococcus faecalis: A Comparative Study
Ruijie Li, Lintian Yuan, Weiqian Jia, Man Qin, Yuguang Wang
Lasers in Surgery and Medicine.2021; 53(3): 400. CrossRef - HEMA-induced oxidative stress inhibits NF-κB nuclear translocation and TNF release from LTA- and LPS-stimulated immunocompetent cells
Helmut Schweikl, Margaritha Birke, Marialucia Gallorini, Christine Petzel, Carola Bolay, Claudia Waha, Karl-Anton Hiller, Wolfgang Buchalla
Dental Materials.2021; 37(1): 175. CrossRef - Lactobacillus rhamnosus and Lactobacillus casei Affect Various Stages of Gardnerella Species Biofilm Formation
Yuanhui He, Risu Na, Xiaoxi Niu, Bingbing Xiao, Huixia Yang
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Cell-Free Lactobacillus sp Supernatants Modulate Staphylococcus aureus Growth, Adhesion and Invasion to Human Osteoblast (HOB) Cells
Fatma Kalaycı Yüksek, Defne Gümüş, Gül İpek Gündoğan, Mine Anğ Küçüker
Current Microbiology.2021; 78(1): 125. CrossRef - Comparison of solithromycin with erythromycin in Enterococcus faecalis and Enterococcus faecium from China: antibacterial activity, clonality, resistance mechanism, and inhibition of biofilm formation
Yu Wang, Yanpeng Xiong, Zhanwen Wang, Jinxin Zheng, Guangjian Xu, Qiwen Deng, Zewen Wen, Zhijian Yu
The Journal of Antibiotics.2021; 74(2): 143. CrossRef - Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef - Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Dongwook Lee, Jintaek Im, Dong Hyun Park, Sungho Jeong, Miri Park, Seokmin Yoon, Jaewoong Park, Seung Hyun Han
Frontiers in Microbiology.2021;[Epub] CrossRef - Can Probiotics Emerge as Effective Therapeutic Agents in Apical Periodontitis? A Review
Gaurav Kumar, Sanjay Tewari, John Tagg, Michael Leonidas Chikindas, Igor V Popov, Santosh Kumar Tiwari
Probiotics and Antimicrobial Proteins.2021; 13(2): 299. CrossRef - Lactobacilli as Anti-biofilm Strategy in Oral Infectious Diseases: A Mini-Review
Barbara Giordani, Carola Parolin, Beatrice Vitali
Frontiers in Medical Technology.2021;[Epub] CrossRef - Interplay between Candida albicans and Lactic Acid Bacteria in the Gastrointestinal Tract: Impact on Colonization Resistance, Microbial Carriage, Opportunistic Infection, and Host Immunity
Karen D. Zeise, Robert J. Woods, Gary B. Huffnagle
Clinical Microbiology Reviews.2021;[Epub] CrossRef - Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods
Basavaprabhu H. Nataraj, Syed Azmal Ali, Pradip V. Behare, Hariom Yadav
Microbial Cell Factories.2020;[Epub] CrossRef - Vaginal microbiota and the potential of Lactobacillus derivatives in maintaining vaginal health
Wallace Jeng Yang Chee, Shu Yih Chew, Leslie Thian Lung Than
Microbial Cell Factories.2020;[Epub] CrossRef - Enhanced biofilm formation of Streptococcus gordonii with lipoprotein deficiency
Ok‐Jin Park, Solmin Jung, Taehwan Park, A Reum Kim, Dongwook Lee, Hyun Jung Ji, Ho Seong Seo, Cheol‐Heui Yun, Seung Hyun Han
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A. Reum Kim, Minji Kang, Yeon-Jee Yoo, Cheol-Heui Yun, Hiran Perinpanayagam, Kee-Yeon Kum, Seung Hyun Han
Journal of Microbiology.2020; 58(4): 314. CrossRef - Streptococcus gordonii: Pathogenesis and Host Response to Its Cell Wall Components
Ok-Jin Park, Yeongkag Kwon, Chaeyeon Park, Yoon Ju So, Tae Hwan Park, Sungho Jeong, Jintaek Im, Cheol-Heui Yun, Seung Hyun Han
Microorganisms.2020; 8(12): 1852. CrossRef - Using Probiotics as Supplementation for Helicobacter pylori Antibiotic Therapy
Jianfu Ji, Hong Yang
International Journal of Molecular Sciences.2020; 21(3): 1136. CrossRef - In-vitro effect of vaginal lactobacilli against group B Streptococcus
Giacomo Marziali, Claudio Foschi, Carola Parolin, Beatrice Vitali, Antonella Marangoni
Microbial Pathogenesis.2019; 136: 103692. CrossRef
- Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine
-
Min Yong Jwa , Soyoung Jeong , Eun Byeol Ko , A Reum Kim , Hyun Young Kim , Sun Kyung Kim , Ho Seong Seo , Cheol-Heui Yun , Seung Hyun Han
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J. Microbiol. 2018;56(8):579-585. Published online July 25, 2018
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DOI: https://doi.org/10.1007/s12275-018-8347-1
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15
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Abstract
-
Streptococcus pneumoniae is a major respiratory pathogen
that causes millions of deaths worldwide. Although subunit
vaccines formulated with the capsular polysaccharides or
their protein conjugates are currently-available, low-cost
vaccines with wide serotype coverage still remain to be developed,
especially for developing countries. Recently, gamma-
irradiation has been considered as an effective inactivation
method
to prepare S. pneumoniae vaccine candidate.
In this study, we investigated the immunogenicity and protective
immunity of gamma-irradiated S. pneumoniae (r-SP),
by comparing with heat-inactivated S. pneumoniae (h-SP)
and formalin-inactivated S. pneumoniae (f-SP), both of which
were made by traditional inactivation methods. Intranasal
immunization of C57BL/6 mice with r-SP in combination
with cholera toxin as an adjuvant enhanced S. pneumoniaespecific
antibodies on the airway mucosal surface and in sera
more potently than that with h-SP or f-SP under the same
conditions. In addition, sera from mice immunized with r-
SP potently induced opsonophagocytic killing activity more
effectively than those of h-SP or f-SP, implying that r-SP
could induce protective antibodies. Above all, immunization
with r-SP effectively protected mice against S. pneumoniae
infection. Collectively, these results suggest that gamma-
irradiation is an effective method for the development
of a killed whole cell pneumococcal vaccine that elicits robust
mucosal and systemic immune responses.
-
Citations
Citations to this article as recorded by - Acute otitis media pneumococcal disease burden and nasopharyngeal colonization in children due to serotypes included and not included in current and new pneumococcal conjugate vaccines
Michael Pichichero, Richard Malley, Ravinder Kaur, Robert Zagursky, Porter Anderson
Expert Review of Vaccines.2023; 22(1): 118. CrossRef - Knock-down of IGFBP2 ameliorates lung fibrosis and inflammation in rats with severe pneumonia through STAT3 pathway
Yuyu Wang, Jianjiang Huang, Fang Zhang, Keli Shen, Bin Qiu
Growth Factors.2023; 41(4): 210. CrossRef - Occurrence of influenza and bacterial infections in cancer patients receiving radiotherapy in Ghana
Augustina K. Arjarquah, Evangeline Obodai, Hannah Ayettey Anie, Michael Aning Osei, John Kofi Odoom, Joseph H. K. Bonney, Eric Behene, Erasmus N. Kotey, James Aboagye, Stephen O. Nyarko, Jeannette Bentum, Clara Yeboah, Selassie Kumordjie, Bright Agbodzi,
PLOS ONE.2022; 17(7): e0271877. CrossRef - Low-Energy Electron Irradiation of Tick-Borne Encephalitis Virus Provides a Protective Inactivated Vaccine
Julia Finkensieper, Leila Issmail, Jasmin Fertey, Alexandra Rockstroh, Simone Schopf, Bastian Standfest, Martin Thoma, Thomas Grunwald, Sebastian Ulbert
Frontiers in Immunology.2022;[Epub] CrossRef - Non-capsular based immunization approaches to prevent Streptococcus pneumoniae infection
Pedro H. Silva, Yaneisi Vázquez, Camilo Campusano, Angello Retamal-Díaz, Margarita K. Lay, Christian A. Muñoz, Pablo A. González, Alexis M. Kalergis, Susan M. Bueno
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - A Nonadjuvanted Whole-Inactivated Pneumococcal Vaccine Induces Multiserotype Opsonophagocytic Responses Mediated by Noncapsule-Specific Antibodies
Shannon C. David, Erin B. Brazel, Eve V. Singleton, Vikrant Minhas, Zoe Laan, Catherine Scougall, Austen Y. Chen, Hui Wang, Chloe J. Gates, Kimberley T. McLean, Jeremy S. Brown, Giuseppe Ercoli, Rachel A. Higgins, Paul V. Licciardi, Kim Mulholland, Justin
mBio.2022;[Epub] CrossRef - Developing green and sustainable concrete in integrating with different urban wastes
Huaguo Chen, Cheuk Lun Chow, Denvid Lau
Journal of Cleaner Production.2022; 368: 133057. CrossRef - Intranasal Vaccine Delivery Technology for Respiratory Tract Disease Application with a Special Emphasis on Pneumococcal Disease
William Walkowski, Justin Bassett, Manmeet Bhalla, Blaine A. Pfeifer, Elsa N. Bou Ghanem
Vaccines.2021; 9(6): 589. CrossRef - Immune Responses to Irradiated Pneumococcal Whole Cell Vaccine
Eunbyeol Ko, Soyoung Jeong, Min Yong Jwa, A Reum Kim, Ye-Eun Ha, Sun Kyung Kim, Sungho Jeong, Ki Bum Ahn, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Vaccines.2021; 9(4): 405. CrossRef - Controlling the Colonization of Clostridium perfringens in Broiler Chickens by an Electron-Beam-Killed Vaccine
Palmy R. Jesudhasan, Sohini S. Bhatia, Kirthiram K. Sivakumar, Chandni Praveen, Kenneth J. Genovese, Haiqi L. He, Robert Droleskey, Jack L. McReynolds, James A. Byrd, Christina L. Swaggerty, Michael H. Kogut, David J. Nisbet, Suresh D. Pillai
Animals.2021; 11(3): 671. CrossRef - Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities
Jasmin Fertey, Martin Thoma, Jana Beckmann, Lea Bayer, Julia Finkensieper, Susann Reißhauer, Beatrice Sarah Berneck, Leila Issmail, Jessy Schönfelder, Javier Portillo Casado, Andre Poremba, Frank-Holm Rögner, Bastian Standfest, Gustavo R. Makert, Lia Walc
Scientific Reports.2020;[Epub] CrossRef - Innate and Adaptive Immune Responses against Bordetella pertussis and Pseudomonas aeruginosa in a Murine Model of Mucosal Vaccination against Respiratory Infection
Catherine B. Blackwood, Emel Sen-Kilic, Dylan T. Boehm, Jesse M. Hall, Melinda E. Varney, Ting Y. Wong, Shelby D. Bradford, Justin R. Bevere, William T. Witt, F. Heath Damron, Mariette Barbier
Vaccines.2020; 8(4): 647. CrossRef - Low-Energy Electron Irradiation Efficiently Inactivates the Gram-Negative Pathogen Rodentibacter pneumotropicus—A New Method for the Generation of Bacterial Vaccines with Increased Efficacy
Jasmin Fertey, Lea Bayer, Sophie Kähl, Rukiya M. Haji, Anke Burger-Kentischer, Martin Thoma, Bastian Standfest, Jessy Schönfelder, Javier Portillo Casado, Frank-Holm Rögner, Christoph Georg Baums, Thomas Grunwald, Sebastian Ulbert
Vaccines.2020; 8(1): 113. CrossRef - Next-Generation Whole-Cell Pneumococcal Vaccine
Victor Morais, Esther Texeira, Norma Suarez
Vaccines.2019; 7(4): 151. CrossRef - Gamma-irradiation-killed Streptococcus pneumoniae potently induces the expression of IL-6 and IL-8 in human bronchial epithelial cells
Min Yong Jwa, Eun Byeol Ko, Hyun Young Kim, Sun Kyung Kim, Soyoung Jeong, Ho Seong Seo, Cheol-Heui Yun, Seung Hyun Han
Microbial Pathogenesis.2018; 124: 38. CrossRef
- Guinea pig complement potently measures vibriocidal activity of human antibodies in response to cholera vaccines
-
Kyoung Whun Kim , Soyoung Jeong , Ki Bum Ahn , Jae Seung Yang , Cheol-Heui Yun , Seung Hyun Han
-
J. Microbiol. 2017;55(12):973-978. Published online December 7, 2017
-
DOI: https://doi.org/10.1007/s12275-017-7478-0
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68
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3
Crossref
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Abstract
-
The vibriocidal assay using guinea pig complement is widely
used for the evaluation of immune responses to cholera vaccines
in human clinical trials. However, it is unclear why
guinea pig complement has been used over human complement
in the measurement of vibriocidal activity of human
sera and there have not been comparison studies for the use
of guinea pig complement over those from other species.
Therefore, we comparatively investigated the effects of complements
derived from human, guinea pig, rabbit, and sheep
on vibriocidal activity. Complements from guinea pig, rabbit,
and human showed concentration-dependent vibriocidal activity
in the presence of quality control serum antibodies. Of
these complements, guinea pig complement was the most sensitive
and effective over a wide concentration range. When
the vibriocidal activity of complements was measured in the
absence of serum antibodies, human, sheep, and guinea pig
complements showed vibriocidal activity up to 40-fold, 20-
fold, and 1-fold dilution, respectively. For human pre- and
post-vaccination sera, the most potent vibriocidal activity was
observed when guinea pig complement was used. In addition,
the highest fold-increases between pre- and post- vaccinated
sera were obtained with guinea pig complement. Furthermore,
human complement contained a higher amount
of V. cholerae- and its lipopolysaccharide-specific antibodies
than guinea pig complement. Collectively, these results suggest
that guinea pig complements are suitable for vibriocidal
assays due to their high sensitivity and effectiveness to human
sera.
-
Citations
Citations to this article as recorded by - Immunogenicity and protective efficacy of a live, oral cholera vaccine formulation stored outside-the-cold-chain for 140 days
Tew Hui Xian, Kurunathan Sinniah, Chan Yean Yean, Venkateskumar Krishnamoorthy, Mohd Baidi Bahari, Manickam Ravichandran, Guruswamy Prabhakaran
BMC Immunology.2020;[Epub] CrossRef - A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation
Stephanie Fischinger, Jonathan K. Fallon, Ashlin R. Michell, Thomas Broge, Todd J. Suscovich, Hendrik Streeck, Galit Alter
Journal of Immunological Methods.2019; 473: 112630. CrossRef - Characterization of antibody response in patients with acute and chronic chikungunya virus disease
Fatih Anfasa, Stephanie M. Lim, Susan Fekken, Robert Wever, Albert D.M.E. Osterhaus, Byron E.E. Martina
Journal of Clinical Virology.2019; 117: 68. CrossRef
- The synthetic human beta-defensin-3 C15 peptide exhibits antimicrobial activity against Streptococcus mutans, both alone and in combination with dental disinfectants
-
Ki Bum Ahn , A Reum Kim , Kee-Yeon Kum , Cheol-Heui Yun , Seung Hyun Han
-
J. Microbiol. 2017;55(10):830-836. Published online September 28, 2017
-
DOI: https://doi.org/10.1007/s12275-017-7362-y
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77
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0
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27
Crossref
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Abstract
-
Streptococcus mutans is a major etiologic agent of human
dental caries that forms biofilms on hard tissues in the human
oral cavity, such as tooth and dentinal surfaces. Human
β-defensin-3 (HBD3) is a 45-amino-acid natural antimicrobial
peptide that has broad spectrum antimicrobial activity
against bacteria and fungi. A synthetic peptide consisting of
the C-terminal 15 amino acids of HBD3 (HBD3-C15) was
recently shown to be sufficient for its antimicrobial activity.
Thus, clinical applications of this peptide have garnered
attention. In this study, we investigated whether HBD3-C15
inhibits the growth of the representative cariogenic pathogen
Streptococcus mutans and its biofilm formation. HBD3-C15
inhibited bacterial growth, exhibited bactericidal activity,
and attenuated bacterial biofilm formation in a dose-dependent
manner. HBD3-C15 potentiated the bactericidal and
anti-biofilm activity of calcium hydroxide (CH) and chlorhexidine
digluconate (CHX), which are representative disinfectants
used in dental clinics, against S. mutans. Moreover,
HBD3-C15 showed antimicrobial activity by inhibiting biofilm
formation by S. mutans and other dentinophilic bacteria
such as Enterococcus faecalis and Streptococcus gordonii,
which are associated with dental caries and endodontic
infection, on human dentin slices. These effects were observed
for HBD3-C15 alone and for HBD3-C15 in combination with
CH or CHX. Therefore, we suggest that HBD3-C15 is a potential
alternative or additive disinfectant that can be used
for the treatment of oral infectious diseases, including dental
caries and endodontic infections.
-
Citations
Citations to this article as recorded by - Human β-defensins and their synthetic analogs: Natural defenders and prospective new drugs of oral health
Mumian Chen, Zihe Hu, Jue Shi, Zhijian Xie
Life Sciences.2024; 346: 122591. CrossRef - Addressing Antimicrobial Properties in Guided Tissue/Bone Regeneration Membrane: Enhancing Effectiveness in Periodontitis Treatment
Sara Takallu, Esmaeil Mirzaei, Abbas Zakeri Bazmandeh, Hamid Reza Ghaderi Jafarbeigloo, Hooman Khorshidi
ACS Infectious Diseases.2024; 10(3): 779. CrossRef - Novel Nano Drug‐Loaded Hydrogel Coatings for the Prevention and Treatment of CAUTI
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Xinzi Kong, Vijetha Vishwanath, Prasanna Neelakantan, Zhou Ye
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Karthikeyan Kandaswamy, Raghunandhakumar Subramanian, Jayant Giri, Ajay Guru, Jesu Arockiaraj
International Journal of Peptide Research and Therapeutics.2024;[Epub] CrossRef - A Novel Beta-Defensin Isoform from Malabar Trevally, Carangoides malabaricus (Bloch & Schneider, 1801), an Arsenal Against Fish Bacterial Pathogens: Molecular Characterization, Recombinant Production, and Mechanism of Action
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Sehyeok Im, Jun Hyuck Lee, Youn-Soo Shim
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Mayron Alves Vasconcelos, Bruno Rocha da Silva, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Francisco Flávio Vasconcelos Evaristo, Francisco Vassiliepe Sousa Arruda, Esteban Nicolás Lorenzón, Eduardo Maffud Cilli, Edson Holanda Teixeira
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Olivia Lili Zhang, John Yun Niu, Ollie Yiru Yu, May Lei Mei, Nicholas Stephen Jakubovics, Chun Hung Chu
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Albert Donald Luong, Alyah Buzid, John H. T. Luong
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Enterococcus faecium DB1 on Cariogenic
Streptococcus mutans Biofilm Formation
Ni-Na Kim, Bong Sun Kim, Han Bin Lee, Sunghyun An, Donghan Kim, Seok-Seong Kang
Food Science of Animal Resources.2022; 42(6): 1020. CrossRef - In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae
Yankang Wu, Na Yang, Ruoyu Mao, Ya Hao, Da Teng, Jianhua Wang
Microorganisms.2022; 10(5): 881. CrossRef - Characterization of Histopathology and Microbiota in Contemporary Regenerative Endodontic Procedures: Still Coming up Short
Yeon-Jee Yoo, Hiran Perinpanayagam, Youngnim Choi, Yu Gu, Seok-Woo Chang, Seung-Ho Baek, Qiang Zhu, Ashraf F. Fouad, Kee-Yeon Kum
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- Sublingual Administration of Bacteria-Expressed Influenza Virus Hemagglutinin 1 (HA1) Induces Protection against Infection with 2009 Pandemic H1N1 Influenza Virus
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Byoung-Shik Shim , Jung-ah Choi , Ho-Hyun Song , Sung-Moo Park , In Su Cheon , Ji-Eun Jang , Sun Je Woo , Chung Hwan Cho , Min-Suk Song , Hyemi Kim , Kyung Joo Song , Jae Myun Lee , Suhng Wook Kim , Dae Sub Song , Young Ki Choi , Jae-Ouk Kim , Huan Huu Nguyen , Dong Wook Kim , Young Yil Bahk , Cheol-Heui Yun , Man Ki Song
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J. Microbiol. 2013;51(1):130-135. Published online March 2, 2013
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DOI: https://doi.org/10.1007/s12275-013-2399-z
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Abstract
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Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a wellestablished bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
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