Genetic Diversity and Structure of Cordyceps sinensis Populations from Extensive Geographical Regions in China as Revealed by Inter-Simple Sequence Repeat Markers

Hong-Hui Liang; Zhou Cheng; Xiao-Ling Yang; Shan Li; Zu-Quan Ding; Tong-Shui Zhou; Wen-Ju Zhang; Jia-Kuan Chen

doi:10.1007/s12275-008-0107-1

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Research Support, Non-U.S. Gov't
Genetic Diversity and Structure of Cordyceps sinensis Populations from Extensive Geographical Regions in China as Revealed by Inter-Simple Sequence Repeat Markers: Hong-Hui Liang ¹, Zhou Cheng ¹, Xiao-Ling Yang ¹, Shan Li ¹, Zu-Quan Ding ¹, Tong-Shui Zhou ², Wen-Ju Zhang ², Jia-Kuan Chen ²; Journal of Microbiology 2008;46(5):549-556.
DOI: https://doi.org/10.1007/s12275-008-0107-1
Published online: October 31, 2008

¹School of Life Science and Technology, Tongji University, Shanghai 200092, P. R. China, ²Institute of Biodiversity Science, School of Life Science, Fudan University, Chinese Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering, Shanghai 200433, P. R. China

Corresponding author: Zhou Cheng , Tel: 86-21-6598-5185,

Received: 25 April 2008 • Accepted: 15 July 2008

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Abstract

Cordyceps sinensis is one of the most valuable medicinal caterpillar fungi native to China. However, its productivity is extremely limited and the species is becoming endangered. The genetic diversity of eighteen C. sinensis populations across its major distributing regions in China was evaluated by inter-simple sequence repeat (ISSR) markers. A total of 141 markers were produced in 180 individuals from the 18 populations, of which 99.3% were polymorphic. The low average of Shannon (0.104) and Nei index (0.07) of the 18 populations indicates that there are little genetic variations within populations. For all 18 populations, estimates of total gene diversity (HT), gene diversity within populations (HS), coefficient of genetic differentiation (GST), and gene flow (Nm) were 0.170, 0.071, 0.583, and 0.357, respectively. This pattern suggests that the genetic diversity of C. sinensis is low and most of the ISSR variations are found among populations with little gene exchange. The 18 populations are divided into five groups based on the genetic distance and the grouping pattern matches with the geographic distribution along the latitudinal gradient. The five groups show obvious difference in the GST and Nm values. Therefore, the genetic diversification of C. sinensis populations may be determined by geographic isolation and the combined effects of life history characters and the interaction with host insect species. The information illustrated by this study is useful for selecting in situ conservation sites of C. sinensis.

Keywords: Cordyceps sinensis;genetic diversity;genetic structure;ISSR;geographical distribution

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Genetic Diversity and Structure of Cordyceps sinensis Populations from Extensive Geographical Regions in China as Revealed by Inter-Simple Sequence Repeat Markers

J. Microbiol. 2008;46(5):549-556. Published online October 31, 2008

DOI: https://doi.org/10.1007/s12275-008-0107-1

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