Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia halobia DSM 20541

Bo Zhang; Xin Bao; Lei Wang; Su Sheng Yang

doi:10.1007/s12275-008-0001-x

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Research Support, Non-U.S. Gov't
Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia halobia DSM 20541: Bo Zhang , Xin Bao , Lei Wang , Su Sheng Yang; Journal of Microbiology 2008;46(3):309-318.
DOI: https://doi.org/10.1007/s12275-008-0001-x
Published online: July 5, 2008

Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, Beijing 100193, P. R. China

Corresponding author: Su Sheng Yang , Tel: 86-10-6273-7827,

Received: 25 December 2007 • Accepted: 26 March 2008

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Abstract

The ectABC genes encoding the biosynthesis of ectoine were identified from Nesterenkonia halobia DSM 20541. The intergenic regions of the ectABC genes from N. halobia DSM 20541 were more loosely spaced than those that had been reported before. The amino acid sequence deduced from ectABC of the strain was highly homologous to the EctABC of Brevibacterium linens BL2 (EctA 50%, EctB 70%, and EctC 68% identities). The osmoprotection of ectABC was studied in the Escherichia coli KNabc and E. coli XL1-Blue. The results revealed that ectABC could shorten the lag phase and enhance the final OD600 of E. coli XL1-Blue in MM63 medium containing 0.68 M NaCl, and could initiate KNabc growth in 0.2 M NaCl. Ectoine was proven to be accumulated in E. coli KNabc/pGEM-Nect using HPLC-UV, and validated by LC-MSD-Trap-VL.

Keywords: ectABC gene cluster;Nesterenkonia halobia DSM 20541;osmoprotection

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Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia halobia DSM 20541

J. Microbiol. 2008;46(3):309-318. Published online July 5, 2008

DOI: https://doi.org/10.1007/s12275-008-0001-x

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