Abstract
The gene encoding the Edwardsiella tarda ferric uptake regulator (FurEt) was cloned from a pathogenic E. tarda strain isolated from diseased fish. FurEt shares 90% overall sequence identity with the Escherichia coli Fur (FurEc) and was able to complement the mutant phenotype of a furEc-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated FurEt whereas E112K mutation resulted in a superactive FurEt variant. FurEt negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.
Citations
Citations to this article as recorded by
- High throughput proteomic analysis of Labeo rohita liver infected with Edwardsiella tarda
Nevil Pinto, Mehar Un Nissa, Mujahidkhan A. Pathan, B.S. Yashwanth, M.G. Pratapa, Sanjeeva Srivastava, Mukunda Goswami
Aquaculture.2023; 569: 739338. CrossRef - The Mutation of the DNA-Binding Domain of Fur Protein Enhances the Pathogenicity of Edwardsiella piscicida via Inducing Overpowering Pyroptosis
Mimi Niu, Zhihai Sui, Guoquan Jiang, Ling Wang, Xuemei Yao, Yonghua Hu
Microorganisms.2023; 12(1): 11. CrossRef - Natural 5-Aminolevulinic Acid: Sources, Biosynthesis, Detection and Applications
Meiru Jiang, Kunqiang Hong, Yufeng Mao, Hongwu Ma, Tao Chen, Zhiwen Wang
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Flavobacterium columnare ferric iron uptake systems are required for virulence
Rachel A. Conrad, Jason P. Evenhuis, Ryan S. Lipscomb, David Pérez-Pascual, Rebecca J. Stevick, Clayton Birkett, Jean-Marc Ghigo, Mark J. McBride
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Acid resistance system CadBA is implicated in acid tolerance and biofilm formation and is identified as a new virulence factor of Edwardsiella tarda
Chunmei Du, Xiaoping Huo, Hanjie Gu, Dongmei Wu, Yonghua Hu
Veterinary Research.2021;[Epub] CrossRef - Cu2+ regulated sulfonamides resistance gene (sul) via reactive oxygen species induced ArcA in a pathogenic Vibrio splendidus
Huijie Liu, Tongxiang Song, Tengteng Lv, Xuelin Zhao, Yina Shao, Chenghua Li, Weiwei Zhang
Annals of Microbiology.2019; 69(8): 829. CrossRef - Thioredoxin H (TrxH) contributes to adversity adaptation and pathogenicity of Edwardsiella piscicida
Bi-ying Wang, Hui-qin Huang, Shuang Li, Ping Tang, Hao-fu Dai, Jian-an Xian, Dong-mei Sun, Yong-hua Hu
Veterinary Research.2019;[Epub] CrossRef - HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida
Yan-Jie Shi, Qing-Jian Fang, Hui-Qin Huang, Chun-Guang Gong, Yong-Hua Hu
Veterinary Research.2019;[Epub] CrossRef - Ferric Uptake Regulator (FurA) is Required forAcidovorax citrulliVirulence on Watermelon
Jun Liu, Yanli Tian, Yuqiang Zhao, Rong Zeng, Baohui Chen, Baishi Hu, Ron R. Walcott
Phytopathology®.2019; 109(12): 1997. CrossRef - Variation on a theme: investigating the structural repertoires used by ferric uptake regulators to control gene expression
Sabina Sarvan, James Butcher, Alain Stintzi, Jean-François Couture
BioMetals.2018; 31(5): 681. CrossRef - Functional Analysis of the Ferric Uptake Regulator Gene fur in Xanthomonas vesicatoria
Huiqin Liu, Chunling Dong, Tingchang Zhao, Jucai Han, Tieling Wang, Xiangzhen Wen, Qi Huang, Roy Martin Roop
PLOS ONE.2016; 11(2): e0149280. CrossRef - The global regulatory effect of Edwardsiella tarda Fur on iron acquisition, stress resistance, and host infection: A proteomics-based interpretation
Yong-hua Hu, Li Sun
Journal of Proteomics.2016; 140: 100. CrossRef - Interplay between iron homeostasis and virulence: Fur and RyhB as major regulators of bacterial pathogenicity
Gaëlle Porcheron, Charles M. Dozois
Veterinary Microbiology.2015; 179(1-2): 2. CrossRef - Protein expression and transcription profiles of three strains of Aeromonas salmonicida ssp. salmonicida under normal and iron-limited culture conditions
Simon Menanteau-Ledouble, Julia Kattlun, Katharina Nöbauer, Mansour El-Matbouli
Proteome Science.2014;[Epub] CrossRef - Phenotypic traits, virulence-associated gene profile and genetic relatedness of Edwardsiella tarda
isolates from Japanese eel Anguilla japonica
in Korea
K.I. Kim, J.Y. Kang, J.Y. Park, S.J. Joh, H.S. Lee, Y.K. Kwon
Letters in Applied Microbiology.2014; 58(2): 168. CrossRef - Role of Fur on cyanide tolerance of Pseudomonas pseudoalcaligenes CECT5344
Gracia Becerra, Rafael Blasco, Alberto Quesada, Faustino Merchán, M. Isabel Igeño
Biochemical Society Transactions.2011; 39(6): 1854. CrossRef - Two-component PhoB-PhoR Regulatory System and Ferric Uptake Regulator Sense Phosphate and Iron to Control Virulence Genes in Type III and VI Secretion Systems of Edwardsiella tarda
Smarajit Chakraborty, J. Sivaraman, Ka Yin Leung, Yu-Keung Mok
Journal of Biological Chemistry.2011; 286(45): 39417. CrossRef - Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain
Yun Sun, Chun-sheng Liu, Li Sun
Vaccine.2010; 28(38): 6344. CrossRef - RmpA Regulation of Capsular Polysaccharide Biosynthesis inKlebsiella pneumoniaeCG43
H. Y. Cheng, Y. S. Chen, C. Y. Wu, H. Y. Chang, Y. C. Lai, H. L. Peng
Journal of Bacteriology.2010; 192(12): 3144. CrossRef - Mutations of ferric uptake regulator (fur) impair iron homeostasis, growth, oxidative stress survival, and virulence of Xanthomonas campestris pv. campestris
Thichakorn Jittawuttipoka, Ratiboot Sallabhan, Paiboon Vattanaviboon, Mayuree Fuangthong, Skorn Mongkolsuk
Archives of Microbiology.2010; 192(5): 331. CrossRef - Overexpression of FurA in Anabaena sp. PCC 7120 Reveals New Targets for This Regulator Involved in Photosynthesis, Iron Uptake and Cellular Morphology
Andrés González, M. Teresa Bes, François Barja, M. Luisa Peleato, María F. Fillat
Plant and Cell Physiology.2010; 51(11): 1900. CrossRef - This Is Not Your Mother's Repressor: the Complex Role of Fur in Pathogenesis
Beth M. Carpenter, Jeannette M. Whitmire, D. Scott Merrell
Infection and Immunity.2009; 77(7): 2590. CrossRef - Domain analysis of the Edwardsiella tarda ferric uptake regulator
Kun Sun, Shuang Cheng, Fang Wang, Li Sun
The Journal of General and Applied Microbiology.2009; 55(5): 351. CrossRef - Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish
Xin Wang, Qiyao Wang, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang
Fish & Shellfish Immunology.2009; 27(3): 469. CrossRef - Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain
Kun Sun, Hua-lei Wang, Min Zhang, Zhi-zhong Xiao, Li Sun
Aquaculture.2009; 289(1-2): 134. CrossRef - Attenuation ofEdwardsiella tardaVirulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing
Min Zhang, Xu-dong Jiao, Yong-hua Hu, Li Sun
Applied and Environmental Microbiology.2009; 75(12): 3882. CrossRef - Identification and immunoprotective analysis of an in vivo-induced Edwardsiella tarda antigen
Xu-dong Jiao, Wei Dang, Yong-hua Hu, Li Sun
Fish & Shellfish Immunology.2009; 27(5): 633. CrossRef - Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine
Huan-ran Wang, Yong-hua Hu, Wei-wei Zhang, Li Sun
Vaccine.2009; 27(30): 4047. CrossRef - A ZnS4 Structural Zinc Site in the Helicobacter pylori Ferric Uptake Regulator
Sylvia Vitale, Caroline Fauquant, David Lascoux, Kristine Schauer, Christine Saint-Pierre, Isabelle Michaud-Soret
Biochemistry.2009; 48(24): 5582. CrossRef - Molecular analysis of the copper-responsive CopRSCD of a pathogenic Pseudomonas fluorescens strain
Yong-hua Hu, Hua-lei Wang, Min Zhang, Li Sun
The Journal of Microbiology.2009; 47(3): 277. CrossRef - Cys-92, Cys-95, and the C-terminal 12 residues of the Vibrio harveyi ferric uptake regulator (Fur) are functionally inessential
Kun Sun, Shuang Cheng, Min Zhang, Fang Wang, Li Sun
The Journal of Microbiology.2008; 46(6): 670. CrossRef
MSK
Cite this Article
