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Research Support, U.S. Gov't, Non-P.H.S.
- Identification of Two-Component Regulatory Genes Involved in o-Xylene Degradation by Rhodococcus sp. Strain DK17
- Dockyu Kim 1,5, Jong-Chan Chae 2, Gerben J. Zylstra 2, Ho-Yong Sohn 3, Gi-Seok Kwon 4, Eungbin Kim 1
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Journal of Microbiology 2005;43(1):49-53.
DOI: https://doi.org/2138 [pii]
1Laboratory of Environmental Microbial Genomics, Department of Biology, Yonsei University, Seoul 120-749, Republic of Korea, 2Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA 3Department of Food and Nutrition, Andong National University, Andong 760-749, Republic of Korea, 4The School of Bioresource Science, Andong National University, Andong 760-749, Republic of Korea, 5Microbial Resources Bank, Microbial Genomics and Applications Center Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Republic of Korea
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Corresponding author:
Eungbin Kim , Tel: 82-2-2123-2651,
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Abstract
Putative genes for a two-component signal transduction system (akbS and akbT) were detected near the alkylbenzene-degrading operon of Rhodococcus sp. DK17. Sequence analysis indicates that AkbS possesses potential ATP-binding and histidine autophosphorylation sites in the N- and C-terminal regions, respectively, and that AkbT has a typical response regulator domain. Mutant analysis combined with RT-PCR experiments further shows that AkbS is required to induce the expression of o-xylene dioxygenase in DK17.
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