Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli

Kang-Lok Lee; Kyung-Chang Lee; Joon-Hee Lee; Jung-Hye Roe

doi:10.1007/s12275-022-1667-1

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Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli: Kang-Lok Lee ¹, Kyung-Chang Lee ², Joon-Hee Lee ³, Jung-Hye Roe ²; Journal of Microbiology 2022;60(4):387-394.
DOI: https://doi.org/10.1007/s12275-022-1667-1
Published online: March 28, 2022

¹Department of Biology Education, IALS, Gyeongsang National University, Jinju 52828, Republic of Korea, ²Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea, ³College of Pharmacy, Pusan National University, Pusan 46241, Republic of Korea

Corresponding author: Kang-Lok Lee ,

Received: 24 December 2021 • Revised: 27 January 2022 • Accepted: 3 February 2022

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Abstract

A reducing system of SoxR, a regulator of redox-active molecules, was identified as rsxABCDGE gene products and RseC in Escherichia coli through genetic studies. We found that ApbE was an additional component of the reducer system. Bacterial two hybrid analysis revealed that these proteins indeed had multiple interactions among themselves. RseC and RsxB formed the core of the complex, interacting with more than five other components. RsxC, the only cytoplasmic component of the system, interacted with SoxR. It might be linked with the rest of the complex via RsxB. Membrane fractions containing the wild type complex but not the mutant complex reduced purified SoxR using NADH as an electron source. These results suggest that Rsx genes, RseC, and ApbE can form a complex using NAD(P)H to reduce SoxR.

Keywords: Fe-S, NADH, RseC, ApbE, Rsx, Rnf

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Characterization of components of a reducing system for SoxR in the cytoplasmic membrane of Escherichia coli

J. Microbiol. 2022;60(4):387-394. Published online March 28, 2022

DOI: https://doi.org/10.1007/s12275-022-1667-1

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