Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity

Young-Il Kim; Mark Anthony B. Casel; Se-Mi Kim; Seong-Gyu Kim; Su-Jin Park; Eun-Ha Kim; Hye Won Jeong; Haryoung Poo; Young Ki Choi

doi:10.1007/s12275-020-0335-6

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Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity: Young-Il Kim ^1,2, Mark Anthony B. Casel ^1,2, Se-Mi Kim ¹, Seong-Gyu Kim ¹, Su-Jin Park ^1,2, Eun-Ha Kim ^1,2, Hye Won Jeong ¹, Haryoung Poo ³, Young Ki Choi ^1,2; Journal of Microbiology 2020;58(10):886-891.
DOI: https://doi.org/10.1007/s12275-020-0335-6
Published online: September 29, 2020

¹College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Republic of Korea, ²Zoonotic Infectious Diseases Research Center, Chungbuk National University, Cheongju 28644, Republic of Korea, ³Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon 34141, Republic of Korea

Corresponding author: Young Ki Choi , Tel: +82-43-261-3384,

Received: 1 July 2020 • Revised: 18 August 2020 • Accepted: 19 August 2020

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Abstract

Various treatments and agents had been reported to inactivate RNA viruses. Of these, thermal inactivation is generally considered an effective and cheap method of sample preparation for downstream assays. The purpose of this study is to establish a safe inactivation method for SARS-CoV-2 without compromising the amount of amplifiable viral genome necessary for clinical diagnoses. In this study, we demonstrate the infectivity and genomic stability of SARSCoV- 2 by thermal inactivation at both 56°C and 65°C. The
results
substantiate that viable SARS-CoV-2 is readily inactivated when incubated at 56°C for 30 min or at 65°C for 10 min. qRT-PCR of specimens heat-inactivated at 56°C for 30 min or 65°C for 15 min revealed similar genomic RNA stability compared with non-heat inactivated specimens. Further, we demonstrate that 30 min of thermal inactivation at 56°C could inactivate viable viruses from clinical COVID-19 specimens without attenuating the qRT-PCR diagnostic sensitivity. Heat treatment of clinical specimens from COVID-19 patients at 56°C for 30 min or 65°C for 15 min could be a useful
method
for the inactivation of a highly contagious agent, SARS-CoV-2. Use of this method would reduce the potential for secondary infections in BSL2 conditions during diagnostic procedures. Importantly, infectious virus can be inactivated in clinical specimens without compromising the sensitivity of the diagnostic RT-PCR assay.

Keywords: SARS-CoV-2, heat inactivation, COVID-19, genomic stability, RT-PCR

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Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity

J. Microbiol. 2020;58(10):886-891. Published online September 29, 2020

DOI: https://doi.org/10.1007/s12275-020-0335-6

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