Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean

Libo Yu; Xixiang Tang; Shiping Wei; Yinkun Qiu; Xiashutong Xu; Guangxin Xu; Qilin Wang; Qian Yang

doi:10.1007/s12275-019-8709-3

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Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean: Libo Yu ^1,2, Xixiang Tang ², Shiping Wei ³, Yinkun Qiu ⁴, Xiashutong Xu ^2,3, Guangxin Xu ^2,3, Qilin Wang ^2,3, Qian Yang ¹; Journal of Microbiology 2019;57(7):562-568.
DOI: https://doi.org/10.1007/s12275-019-8709-3
Published online: June 27, 2019

¹School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150080, P. R. China, ²Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, P. R. China, ³School of Ocean Sciences, China University of Geosciences, Beijing 100083, P. R. China, ⁴Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, P. R. China

Corresponding author: Qian Yang , Tel: +86-592-2195365;,

Received: 28 December 2018 • Revised: 20 February 2019 • Accepted: 20 February 2019

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Abstract

A Gram-positive, aerobic, rod-shaped, spore-forming bacterium, designated YLB-03T, with peritrichous flagella was isolated from deep-sea sediment of the Yap Trench at a depth of 4435 m. The bacterium was found to be catalase-positive but oxidase-negative. Growth of this bacterium was observed at 15–50°C (optimum 37°C), pH 5–10.5 (optimum 7), 0–5% NaCl (optimum 1%, w/v) and 0.1–50 MPa (optimum 0.1 MPa). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YLB-03T was a member of the genus Lysinibacillus. Strain YLB-03T was closely related to Lysinibacillus sinduriensis BLB-1T and Lysinibacillus chungkukjangi 2RL3-2T (98.4%), Lysinibacillus halotolerans LAM- 612T (98.0%), Lysinibacillus telephonicus KT735049T (97.5%), Lysinibacillus endophyticus C9T (97.5%), Lysinibacillus composti NCCP-36T and Lysinibacillus massiliensis 4400831T (97.3%). The ANI and the GGDC DNA-DNA hybridization estimate values between strain YLB-03T and closely related type strains were 73.7–76.3% and 34.7–38.7%, respectively. The principal fatty acids were anteiso-C15:0 and iso-C15:0. The G+C content of the chromosomal DNA was 39.6 mol%. The respiratory quinone was determined to be MK-7. The diagnostic amino acids in the cell wall peptidoglycan contained Lys-Asp (type A4α) and the cell-wall sugars were glucose and xylose. The polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid. The combined genotypic and phenotypic data showed that strain YLB-03T represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus yapensis sp. nov. is proposed, with the type strain YLB-03T (= MCCC 1A12698T = JCM 32871T).

Keywords: Lysinibacillus yapensis sp. nov.;deep-sea sediment;Yap Trench

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Isolation and characterization of a novel piezotolerant bacterium Lysinibacillus yapensis sp. nov., from deep-sea sediment of the Yap Trench, Pacific Ocean

J. Microbiol. 2019;57(7):562-568. Published online June 27, 2019

DOI: https://doi.org/10.1007/s12275-019-8709-3

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