Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains

Grégoire Burgé; Claire Saulou-Bérion; Marwen Moussa; Florent Allais; Violaine Athes; Henry-Eric Spinnler

doi:10.1007/s12275-015-5056-x

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Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains: Grégoire Burgé ^1,2,3, Claire Saulou-Bérion ^2,3, Marwen Moussa ^2,3, Florent Allais ^1,2,3, Violaine Athes ^2,3, Henry-Eric Spinnler ^2,3; Journal of Microbiology 2015;53(10):702-710.
DOI: https://doi.org/10.1007/s12275-015-5056-x
Published online: October 2, 2015

¹Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100 Reims, France, ²AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France, ³INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France¹Chaire Agro-Biotechnologies Industrielles (ABI) - AgroParisTech, 247 rue Paul Vaillant Couturier, F-51100 Reims, France, ²AgroParisTech, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France, ³INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires (GMPA), bâtiment CBAI, 1 avenue Lucien Brétignières, F-78850 Thiverval-Grignon, France

Corresponding author: Henry-Eric Spinnler , Tel: +33-1-30-81-53-87,

Received: 27 January 2015 • Revised: 28 July 2015 • Accepted: 13 August 2015

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Abstract

The aims of this study is to compare the growth and glucose metabolism of three Lactobacillus reuteri strains (i.e. DSM 20016, DSM 17938, and ATCC 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). However, the physiological diversity inside the species, even for basic metabolisms, like its capacity of acidification or glucose metabolism, has not been studied yet. In the present work, the growth and metabolism of three strains representative of the species diversity have been studied in batch mode. The strains were compared through characterization of growth kinetics and evaluation of acidification kinetics, substrate consumption and product formation. The results showed significant differences between the three strains which may be explained, at least in part, by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth: the phosphoketolase or heterolactic pathway (PKP) and the Embden-Meyerhof pathway (EMP). It was also shown that, in the context of obtaining a large amount of biomass, DSM 20016 and DSM 17938 strains were the most effective in terms of growth kinetics. The DSM 17938 strain, which shows the more significant metabolic shift from EMP to PKP when the pH decreases, is more effective for lactate production.

Keywords: Lactobacillus reuteri;microbial growth;acidification kinetics;glucose metabolism;Embden-Meyerhof pathway;Phosphoketolase pathway;lactate production

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Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains

J. Microbiol. 2015;53(10):702-710. Published online October 2, 2015

DOI: https://doi.org/10.1007/s12275-015-5056-x

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