Intestinal Intraepithelial TCRγδ+ T Cells are Activated by Normal Commensal Bacteria

Sang Phil Jeong; Jung-Ah Kang; Sung-Gyoo Park

doi:10.1007/s12275-012-2468-8

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Intestinal Intraepithelial TCRγδ+ T Cells are Activated by Normal Commensal Bacteria: Sang Phil Jeong , Jung-Ah Kang , Sung-Gyoo Park; Journal of Microbiology 2012;50(5):837-841.
DOI: https://doi.org/10.1007/s12275-012-2468-8
Published online: November 4, 2012

School of Life Sciences and Bioimaging Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea

Corresponding author: Sung-Gyoo Park , Tel: +82-62-715-2511,

Received: 31 August 2012 • Accepted: 24 September 2012

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Abstract

TCRγδ+ T cells play a critical role in protecting the intestinal mucosa against pathogenic infection. In the absence of infection, TCRγδ+ T cell activation must be continuously regulated by T regulatory cells (Treg) to prevent the development of colitis. However, the activation of intestinal TCRγδ+ T cells under normal conditions has not been clearly resolved. In order to determine TCRγδ+ T cell activation in vivo, we designed an NF-κB based reporter system. Using the recombinant lentiviral method, we delivered the NF-κB reporter to isolated TCRγδ+ T cells, which were then adoptively transferred into normal mice. Our data indicate that the NF-κB activation level in TCRγδ+ T cells is higher in the intestinal intraepithelial layer than in the lamina propria region. In addition, the surface expression level of lymphocyte activation marker CD69 in TCRγδ+ T cells is also higher in the intestinal intraepithelial layer and this activation was reduced by Sulfatrim treatment which removes of commensal bacteria. Collectively, our data indicate that the TCRγδ+ T cell population attached to the intestinal lumen is constitutively activated even by normal commensal bacteria.

Keywords: TCRγδ+ T cell;intestine;NF-κB;commensal bacteria

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Intestinal Intraepithelial TCRγδ+ T Cells are Activated by Normal Commensal Bacteria

J. Microbiol. 2012;50(5):837-841. Published online November 4, 2012

DOI: https://doi.org/10.1007/s12275-012-2468-8

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