NOTE] Biological and Genetic Properties of SA14-14-2, a Live-Attenuated Japanese Encephalitis Vaccine That Is Currently Available for Humans

Byung-Hak Song; Gil-Nam Yun; Jin-Kyoung Kim; Sang-Im Yun; Young-Min Lee

doi:10.1007/s12275-012-2336-6

Articles

Page Path: HOME > J. Microbiol > Volume 50(4); 2012 > Article

Research Support, Non-U.S. Gov't
NOTE] Biological and Genetic Properties of SA14-14-2, a Live-Attenuated Japanese Encephalitis Vaccine That Is Currently Available for Humans: Byung-Hak Song ¹, Gil-Nam Yun ¹, Jin-Kyoung Kim ¹, Sang-Im Yun ^1,2, Young-Min Lee ^1,2; Journal of Microbiology 2012;50(4):698-706.
DOI: https://doi.org/10.1007/s12275-012-2336-6
Published online: August 25, 2012

¹Department of Microbiology, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea, ²Department of Animal, Dairy and Veterinary Sciences, Utah Science Technology and Research (USTAR), College of Agriculture, Utah State University, Logan, UT 84322-4815, United States of America

Corresponding author: Young-Min Lee , Tel: +1-435-797-9667,

Received: 2 July 2012 • Accepted: 13 July 2012

prev next

323 Views
0 Download
19 Crossref
28 Scopus

Full Article

Download PDF

Abstract

Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, is a major cause of acute encephalitis, a disease of significance for global public health. In the absence of antiviral therapy to treat JEV infection, vaccination is the most effective method of preventing the disease. In JE-endemic areas, the most widely used vaccine to date is SA14-14-2, a live-attenuated virus derived from its virulent parent SA14. In this study, we describe the biological properties of SA14-14-2, both in vitro and in vivo, and report the genetic characteristics of its genomic RNA. In BHK-21 (hamster kidney) cells, SA14-14-2 displayed a slight delay in plaque formation and growth kinetics when compared to a virulent JEV strain, CNU/LP2, with no decrease in maximum virus production. The delay in viral growth was also observed in two other cell lines, SH-SY5Y (human neuroblastoma) and C6/36 (mosquito larva), which are potentially relevant to JEV pathogenesis and transmission. In 3-week-old ICR mice, SA14-14-2 did not cause any symptoms or death after either intracerebral or peripheral inoculation with a maximum dose of up to 1.5×103 plaqueforming units (PFU) per mouse. The SA14-14-2 genome consisted of 10977 nucleotides, one nucleotide longer than all the previously reported genomes of SA14-14-2, SA14 and two other SA14-derived attenuated viruses. This difference was due to an insertion of one G nucleotide at position 10701 in the 3' noncoding region. Also, we noted a significant number of nucleotide and/or amino acid substitutions throughout the genome of SA14-14-2, except for the prM protein-coding region, that differed from SA14 and/or the other two attenuated viruses. Our results, together with others’, provide a foundation not only for the study of JEV virulence but also for the development of new and improved vaccines for JEV.

Keywords: Japanese encephalitis virus;SA14-14-2;complete genome;virulence;pathogenesis

Figure & Data

References

Citations

Citations to this article as recorded by

In Vitro Infection Dynamics of Japanese Encephalitis Virus in Established Porcine Cell Lines
Shakirat A. Adetunji, Dmitriy Smolensky, Dana N. Mitzel, Jeana L. Owens, Carol G. Chitko-McKown, Natalia Cernicchiaro, Leela E. Noronha
Pathogens.2021; 10(11): 1468. CrossRef
Japanese encephalitis virus live attenuated vaccine strains display altered immunogenicity, virulence and genetic diversity
Emily H. Davis, Andrew S. Beck, Li Li, Mellodee M. White, Marianne Banks Greenberg, Jill K. Thompson, Steven G. Widen, Alan D. T. Barrett, Nigel Bourne
npj Vaccines.2021;[Epub] CrossRef
Comprehensive analysis of differential expression profiles via transcriptome sequencing in SH-SY5Y cells infected with CV-A16
Yajie Hu, Zhen Yang, Shenglan Wang, Danxiong Sun, Mingmei Zhong, Mudong Wen, Jie Song, Yunhui Zhang, Juan Carlos de la Torre
PLOS ONE.2020; 15(11): e0241174. CrossRef
Genetic and neuroattenuation phenotypic characteristics and their stabilities of SA14-14-2 vaccine seed virus
Xinyu Liu, Danhua Zhao, Lili Jia, Hongshan Xu, Rui Na, Yonghong Ge, Shaoxiang Liu, Yongxin Yu, Yuhua Li
Vaccine.2018; 36(31): 4650. CrossRef
Differentiated Human SH-SY5Y Cells Provide a Reductionist Model of Herpes Simplex Virus 1 Neurotropism
Mackenzie M. Shipley, Colleen A. Mangold, Chad V. Kuny, Moriah L. Szpara, Rozanne M. Sandri-Goldin
Journal of Virology.2017;[Epub] CrossRef
Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase
TELISSA C. KASSAR, TEREZA MAGALHÃES, JOSÉ V.J. S. JÚNIOR, AMANDA G.O. CARVALHO, ANDRÉA N.M.R. DA SILVA, SABRINA R.A. QUEIROZ, GIOVANI R. BERTANI, LAURA H.V.G. GIL
Anais da Academia Brasileira de Ciências.2017; 89(3 suppl): 2119. CrossRef
Japanese encephalitis virus invasion of cell: allies and alleys
Minu Nain, Malik Z. Abdin, Manjula Kalia, Sudhanshu Vrati
Reviews in Medical Virology.2016; 26(2): 129. CrossRef
Complete Genome Sequences of Three Historically Important, Spatiotemporally Distinct, and Genetically Divergent Strains of Zika Virus: MR-766, P6-740, and PRVABC-59
Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Justin G. Julander, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee
Genome Announcements.2016;[Epub] CrossRef
The Emerging Duck Flavivirus Is Not Pathogenic for Primates and Is Highly Sensitive to Mammalian Interferon Antiviral Signaling
Hong-Jiang Wang, Xiao-Feng Li, Long Liu, Yan-Peng Xu, Qing Ye, Yong-Qiang Deng, Xing-Yao Huang, Hui Zhao, E-De Qin, Pei-Yong Shi, George F. Gao, Cheng-Feng Qin, M. S. Diamond
Journal of Virology.2016; 90(14): 6538. CrossRef
Comparison of the live-attenuated Japanese encephalitis vaccine SA14 -14-2 strain with its pre-attenuated virulent parent SA14 strain: similarities and differences in vitro and in vivo
Sang-Im Yun, Byung-Hak Song, Irina A. Polejaeva, Christopher J. Davies, Kenneth L. White, Young-Min Lee
Journal of General Virology.2016; 97(10): 2575. CrossRef
Genetic Determinants of Japanese Encephalitis Virus Vaccine Strain SA14-14-2 That Govern Attenuation of Virulence in Mice
Gregory D. Gromowski, Cai-Yen Firestone, Stephen S. Whitehead, M. S. Diamond
Journal of Virology.2015; 89(12): 6328. CrossRef
Profiling of Viral Proteins Expressed from the Genomic RNA of Japanese Encephalitis Virus Using a Panel of 15 Region-Specific Polyclonal Rabbit Antisera: Implications for Viral Gene Expression
Jin-Kyoung Kim, Jeong-Min Kim, Byung-Hak Song, Sang-Im Yun, Gil-Nam Yun, Sung-June Byun, Young-Min Lee, Xi Zhou
PLOS ONE.2015; 10(4): e0124318. CrossRef
Extended stability of cyclin D1 contributes to limited cell cycle arrest at G1-phase in BHK-21 cells with Japanese encephalitis virus persistent infection
Ji Young Kim, Soo Young Park, Hey Rhyoung Lyoo, Eung Seo Koo, Man Su Kim, Yong Seok Jeong
Journal of Microbiology.2015; 53(1): 77. CrossRef
A Lentiviral Vector Expressing Japanese Encephalitis Virus-like Particles Elicits Broad Neutralizing Antibody Response in Pigs
Mélissanne de Wispelaere, Meret Ricklin, Philippe Souque, Marie-Pascale Frenkiel, Sylvie Paulous, Obdulio Garcìa-Nicolàs, Artur Summerfield, Pierre Charneau, Philippe Desprès, Fatah Kashanchi
PLOS Neglected Tropical Diseases.2015; 9(10): e0004081. CrossRef
Japanese encephalitis
Sang-Im Yun, Young-Min Lee
Human Vaccines & Immunotherapeutics.2014; 10(2): 263. CrossRef
Japanese encephalitis virus vaccine candidates generated by chimerization with dengue virus type 4
Gregory D. Gromowski, Cai-Yen Firestone, Christopher T. Hanson, Stephen S. Whitehead
Vaccine.2014; 32(25): 3010. CrossRef
Recovery of a chemically synthesized Japanese encephalitis virus reveals two critical adaptive mutations in NS2B and NS4A
Xiao-Dan Li, Xiao-Feng Li, Han-Qing Ye, Cheng-Lin Deng, Qing Ye, Chao Shan, Bao-Di Shang, Lin-Lin Xu, Shi-Hua Li, Sheng-Bo Cao, Zhi-Ming Yuan, Pei-Yong Shi, Cheng-Feng Qin, Bo Zhang
Journal of General Virology .2014; 95(4): 806. CrossRef
A Molecularly Cloned, Live-Attenuated Japanese Encephalitis Vaccine SA14-14-2 Virus: A Conserved Single Amino Acid in the ij Hairpin of the Viral E Glycoprotein Determines Neurovirulence in Mice
Sang-Im Yun, Byung-Hak Song, Jin-Kyoung Kim, Gil-Nam Yun, Eun-Young Lee, Long Li, Richard J. Kuhn, Michael G. Rossmann, John D. Morrey, Young-Min Lee, R. Mark L. Buller
PLoS Pathogens.2014; 10(7): e1004290. CrossRef
Inhibition of japanese encephalitis virus infection by flavivirus recombinant e protein domain III
Jingjing Fan, Yi Liu, Xuping Xie, Bo Zhang, Zhiming Yuan
Virologica Sinica.2013; 28(3): 152. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

NOTE] Biological and Genetic Properties of SA14-14-2, a Live-Attenuated Japanese Encephalitis Vaccine That Is Currently Available for Humans

J. Microbiol. 2012;50(4):698-706. Published online August 25, 2012

DOI: https://doi.org/10.1007/s12275-012-2336-6

Related articles