Shedding of Viral Hemorrhagic Septicemia Virus (Genotype IVb) by Experimentally Infected Muskellunge (Esox masquinongy)

Robert K. Kim; Mohamed Faisal

doi:10.1007/s12275-012-1145-2

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Shedding of Viral Hemorrhagic Septicemia Virus (Genotype IVb) by Experimentally Infected Muskellunge (Esox masquinongy): Robert K. Kim ¹, Mohamed Faisal ^1,2,3; Journal of Microbiology 2012;50(2):278-284.
DOI: https://doi.org/10.1007/s12275-012-1145-2
Published online: April 27, 2012

¹Comparative Medicine and Integrative Biology Program, College of Veterinary Medicine, Michigan State University, MI, USA, ²Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, MI, USA, ³Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, MI, USA

Corresponding author: Mohamed Faisal , Tel: +1-517-884-2019,

Received: 22 March 2011 • Accepted: 4 November 2011

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Abstract

Previous experimental infection demonstrated that juvenile muskellunge (Esox masquinongy) can survive experimental infection of viral hemorrhagic septicemia virus, Genotype IVb (VHSV IVb) at a low concentration of exposure. Herein we report that survivors of experimental infection with VHSV IVb shed the virus into the surrounding environment for an extended period of time. When muskellunge were exposed to VHSV IVb by immersion at a concentration of 1,400 plaque forming units (PFU)/ml, VHSV IVb was detected in the water of surviving fish for up to 15 weeks postexposure (p.e.) with the highest levels of shedding occurring between weeks 1 and 5 p.e. We estimated that each juvenile muskellunge can shed upwards of 1.36×105 PFU/fish/h after initial exposure signifying the uptake and amplification of VHSV to several orders of magnitude above the original exposure concentration. Muskellunge surviving low concentration exposure were re-infected with VHSV IVb by immersion at week 22 p.e. at concentrations ranging from 0 to 106 PFU/ml. Viral shedding was detected in all re-exposed fish, including mock rechallenged controls up to 15 consecutive weeks. Rates of viral shedding were substantially higher following rechallenge in the first 5 weeks. The highest rate of viral shedding was approximately 4.6×106 PFU/fish/h and shedding did not necessarily correspond to the re-exposure VHSV concentration. The results of this study shed new light into the dynamics of VHSV IVb shedding in a highly susceptible host and provide useful insights to fishery managers to design effective control strategies to this deadly virus.

Keywords: viral hemorrhagic septicemia virus;genotype IVb;shedding;muskellunge;rhabdovirus

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Shedding of Viral Hemorrhagic Septicemia Virus (Genotype IVb) by Experimentally Infected Muskellunge (Esox masquinongy)

J. Microbiol. 2012;50(2):278-284. Published online April 27, 2012

DOI: https://doi.org/10.1007/s12275-012-1145-2

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