Abstract
The aim of the present study was to decipher the diversity of methanogens in rumen of Murrah buffaloes
so that effective strategies can be made in order to mitigate methane emission from these methanogens.
In the present study diversity of rumen methanogens in Murrah buffaloes (Bubalus bubalis) from North
India was evaluated by using mcr-A gene library obtained from the pooled PCR product from four animals
and by using MEGA4 software. A total of 104 clones were examined, revealing 26 different mcr-A gene
sequences or phylotypes. Of the 26 phylotypes, 16 (64 of 104 clones) were less than 97% similar to any
of the cultured strain of methanogens. Seven clone sequences were clustered with Methanomicrobium mobile
and three clone sequences were clustered with Methanobrevibacter gottschalkii during the phylogenetic
analysis. Uncultured group of methanogens comes out to be the major component of the methanogens community
structure in Murrah buffaloes. Methanomicrobium phylotype comes out to be major phylotype
among cultured methanogens followed by Methanobrevibacter phylotype. These results help in making effective
strategies to check the growth of dominant methanogenic communities in the rumen of this animal
which in turn help in the reduction of methane emission in the environment and ultimately helps us in
fighting with the problem of global warming.
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