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HOME > J. Microbiol > Volume 49(4); 2011 > Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi
Yuanyuan Chen 1, Bernard A. Prior 2, Guiyang Shi 1, Zhengxiang Wang 1
Journal of Microbiology 2011;49(4):675-679.
DOI: https://doi.org/10.1007/s12275-011-0525-3
Published online: September 2, 2011
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China, 2Department of Microbiology, School of Biological Sciences, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
Corresponding author:  Zhengxiang Wang , Tel: +86-510-85918121, 
Received: 17 December 2010   • Accepted: 18 March 2011
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Abstract

In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.

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    A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi
    J. Microbiol. 2011;49(4):675-679.   Published online September 2, 2011
    DOI: https://doi.org/10.1007/s12275-011-0525-3
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