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HOME > J. Microbiol > Volume 48(1); 2010 > Article
Research Support, Non-U.S. Gov't
The Involvement of the nif-Associated Ferredoxin-Like Genes fdxA and fdxN of Herbaspirillum seropedicae in Nitrogen Fixation
André L.F. Souza 1, Adriana L. Invitti 1, Fabiane G.M. Rego 1, Rose A. Monteiro 1, Giseli Klassen 2, Emanuel M. Souza 1, Leda S. Chubatsu 1, Fábio O. Pedrosa 1, Liu U. Rigo 1
Journal of Microbiology 2010;48(1):77-83.
DOI: https://doi.org/10.1007/s12275-009-0077-y
Published online: March 11, 2010
1Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, Brazil, 2Department of Basic Pathology, Universidade Federal do Paraná, CP 19046, Brazil1Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, CP 19046, Brazil, 2Department of Basic Pathology, Universidade Federal do Paraná, CP 19046, Brazil
Corresponding author:  Liu U. Rigo , Tel: +55-41-3361-1578, 
Received: 13 March 2009   • Accepted: 26 July 2009
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Abstract

The pathway of electron transport to nitrogenase in the endophytic β-Proteobacterium Herbaspirillum seropedicae has not been characterized. We have generated mutants in two nif-associated genes encoding putative ferredoxins, fdxA and fdxN. The fdxA gene is part of the operon nifHDKENXorf1orf2fdxAnifQmodABC and is transcribed from the nifH promoter, as revealed by lacZ gene fusion. The fdxN gene is probably cotranscribed with the nifB gene. Mutational analysis suggests that the FdxA protein is essential for maximum nitrogenase activity, since the nitrogenase activity of the fdxA mutant strain was reduced to about 30% of that of the wild-type strain. In addition, the fdxA mutation had no effect on the nitrogenase switch-off in response to ammonium. Nitrogenase activity of a mutant strain lacking the fdxN gene was completely abolished. This phenotype was reverted by complementation with fdxN expressed under lacZ promoter control. The results suggest that the products of both the fdxA and fdxN genes are probably involved in electron transfer during nitrogen fixation.

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    The Involvement of the nif-Associated Ferredoxin-Like Genes fdxA and fdxN of Herbaspirillum seropedicae in Nitrogen Fixation
    J. Microbiol. 2010;48(1):77-83.   Published online March 11, 2010
    DOI: https://doi.org/10.1007/s12275-009-0077-y
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