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HOME > J. Microbiol > Volume 47(6); 2009 > Article
Research Support, Non-U.S. Gov't
Scopulibacillus darangshiensis gen. nov., sp. nov., Isolated from Rock
Soon Dong Lee , Dong Wan Lee
Journal of Microbiology 2009;47(6):710-715.
DOI: https://doi.org/10.1007/s12275-009-0111-0
Published online: February 4, 2010
Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea
Corresponding author:  Soon Dong Lee , Tel: 82-64-754-3282, 
Received: 9 April 2009   • Accepted: 1 July 2009
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Abstract

A novel, Gram-positive bacterium, designated DLS-06T, was isolated from scoria (volcanic ash) under rock on the peak of small mountain (300 m above the sea level; known as Darangshi Oreum) in Jeju, Republic of Korea. The cells of the isolate were aerobic, oxidase-negative, catalase-positive, endospore- forming, non-motile rods. The organism grew at 25~30°C and initial pH 6.1~9.1. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism was related to members of the family “Sporolactobacillaceae” and related taxa. The phylogenetic neighbours were Pullulanibacillus naganoensis (95.2% 16S rRNA gene sequence similarity), Tuberibacillus calidus (95.0%) and Sporolactobacillus (91.8~94.2%). Levels of 16S rRNA gene sequence similarity of the isolate to representatives of other genera were in the range of 87.2~93.7%. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The predominant menaquinone was MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unknown ninhydrin-positive phospholipid, three unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C15:0 and anteiso-C17:0. The G+C content of the DNA was 50.8 mol%. On the basis of the phenotypic and phylogenetic data presented in this study, this organism represents a novel genus and species in the order Bacillales, for which the name Scopulibacillus darangshiensis gen. nov., sp. nov. is proposed. The type strain is DLS-06T (=DSM 19377T =KCTC 13161T).

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    Scopulibacillus darangshiensis gen. nov., sp. nov., Isolated from Rock
    J. Microbiol. 2009;47(6):710-715.   Published online February 4, 2010
    DOI: https://doi.org/10.1007/s12275-009-0111-0
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