Abstract
Hepatitis B virus (HBV) genome replication requires the packaging of viral factors (pregenomic RNA and polymerase) as well as host factors, including heat shock proteins and protein kinase C. Previous reports have suggested that there are several unidentified host factors that affect this encapsidation step. In this study, we identified a new host factor, nucleophosmin (B23) that interacts with the HBV core protein 149 (Cp149). We analyzed this factor using NHS-activated sepharose resin and MALDI-TOF MS. Using the BIAcore analysis system, we were also able to deduce that the B23.1 residues 259-294 were required for the interaction between Cp149 and B23.1 in vitro.
Citations
Citations to this article as recorded by
- Nucleophosmin: A Nucleolar Phosphoprotein Orchestrating Cellular Stress Responses
Mohamed S. Taha, Mohammad Reza Ahmadian
Cells.2024; 13(15): 1266. CrossRef - Live Cell Imaging Reveals HBV Capsid Translocation from the Nucleus To the Cytoplasm Enabled by Cell Division
Sofia Romero, Nuruddin Unchwaniwala, Edward L. Evans, Kevin W. Eliceiri, Daniel D. Loeb, Nathan M. Sherer, Haitao Guo, Sara Cherry
mBio.2023;[Epub] CrossRef - The Hepatitis B Virus Interactome: A Comprehensive Overview
Ellen Van Damme, Jolien Vanhove, Bryan Severyn, Lore Verschueren, Frederik Pauwels
Frontiers in Microbiology.2021;[Epub] CrossRef - HBV Core Protein Is in Flux between Cytoplasmic, Nuclear, and Nucleolar Compartments
Smita Nair, Adam Zlotnick, Ralf Bartenschlager, Stephen P. Goff
mBio.2021;[Epub] CrossRef - Hepatitis B virus biology and life cycle
Senko Tsukuda, Koichi Watashi
Antiviral Research.2020; 182: 104925. CrossRef - Nucleophosmin in Its Interaction with Ligands
Ilaria Cela, Adele Di Matteo, Luca Federici
International Journal of Molecular Sciences.2020; 21(14): 4885. CrossRef - Implication of B23/NPM1 in Viral Infections, Potential Uses of B23/NPM1 Inhibitors as Antiviral Therapy
Yadira Lobaina, Yasser Perera
Infectious Disorders - Drug Targets.2019; 19(1): 2. CrossRef - Nucleolar Localization of HIV-1 Rev Is Required, Yet Insufficient for Production of Infectious Viral Particles
Jerlisa Ann C. Arizala, Mayumi Takahashi, John C. Burnett, Dominique L. Ouellet, Haitang Li, John J. Rossi
AIDS Research and Human Retroviruses.2018; 34(11): 961. CrossRef - Nucleophosmin (NPM1)/B23 in the Proteome of Human Astrocytic Cells Restricts Chikungunya Virus Replication
Rachy Abraham, Sneha Singh, Sreeja R. Nair, Neha Vijay Hulyalkar, Arun Surendran, Abdul Jaleel, Easwaran Sreekumar
Journal of Proteome Research.2017; 16(11): 4144. CrossRef - Crosstalk between the nucleolus and the DNA damage response
L M Ogawa, S J Baserga
Molecular BioSystems.2017; 13(3): 443. CrossRef - The true story and advantages of the famous Hepatitis B virus core particles: Outlook 2016
P. Pumpens, E. Grens
Molecular Biology.2016; 50(4): 489. CrossRef - Biophysical Characterization of Nucleophosmin Interactions with Human Immunodeficiency Virus Rev and Herpes Simplex Virus US11
Kazem Nouri, Jens M. Moll, Lech-Gustav Milroy, Anika Hain, Radovan Dvorsky, Ehsan Amin, Michael Lenders, Luitgard Nagel-Steger, Sebastian Howe, Sander H. J. Smits, Hartmut Hengel, Lutz Schmitt, Carsten Münk, Luc Brunsveld, Mohammad R. Ahmadian, Michael Ne
PLOS ONE.2015; 10(12): e0143634. CrossRef - NIRF, a Novel Ubiquitin Ligase, Inhibits Hepatitis B Virus Replication Through Effect on HBV Core Protein and H3 Histones
Guanhua Qian, Bin Hu, Danlin Zhou, Yanyan Xuan, Lu Bai, Changzhu Duan
DNA and Cell Biology.2015; 34(5): 327. CrossRef - Bridging the past and the future of virology: Surface plasmon resonance as a powerful tool to investigate virus/host interactions
Marco Rusnati, Paola Chiodelli, Antonella Bugatti, Chiara Urbinati
Critical Reviews in Microbiology.2015; 41(2): 238. CrossRef - High throughput proteomic analysis and a comparative review identify the nuclear chaperone, Nucleophosmin among the common set of proteins modulated in Chikungunya virus infection
Rachy Abraham, Prashant Mudaliar, Abdul Jaleel, Jandhyam Srikanth, Easwaran Sreekumar
Journal of Proteomics.2015; 120: 126. CrossRef - The Impact of Mass Spectrometry–Based Proteomics on Fundamental Discoveries in Virology
Todd M. Greco, Benjamin A. Diner, Ileana M. Cristea
Annual Review of Virology.2014; 1(1): 581. CrossRef - Interaction between nucleophosmin and HBV core protein increases HBV capsid assembly
Heewon Jeong, Min-Hyung Cho, Sung-Gyoo Park, Guhung Jung
FEBS Letters.2014; 588(6): 851. CrossRef - NPM1/B23: A Multifunctional Chaperone in Ribosome Biogenesis and Chromatin Remodeling
Mikael S. Lindström
Biochemistry Research International.2011; 2011: 1. CrossRef - Biosensing applications of surface plasmon resonance-based Biacore technology
Subash C.B. Gopinath
Sensors and Actuators B: Chemical.2010; 150(2): 722. CrossRef
MSK
Cite this Article
