Abstract
This report describes the identification of a novel linear B-cell epitope at the C-terminus of the membrane (M) protein of avian infectious bronchitis virus (IBV). A monoclonal antibody (MAb) (designated as 15E2) against the IBV M protein was prepared and a series of 14 partially-overlapping fragments of the IBV M gene were expressed with a GST tag. These peptides were subjected to enzyme-linked immunosorbent assay (ELISA) and western blotting analysis using MAb 15E2 to identify the epitope. A linear motif, 199FATFVYAK206, which was located at the C-terminus of the M protein, was identified by MAb 15E2. ELISA and western blotting also showed that this epitope could be recognized by IBV-positive serum from
chicken. Given that 15E2 showed reactivity with the 199FATFVYAK206 motif, expressed as a GST fusion protein, in both western blotting and in an ELISA, we proposed that this motif represented a linear B-cell epitope of the M protein. The 199FATFVYAK206 motif was the minimal requirement for reactivity as demonstrated
by analysis of the reactivity of 15E2 with several truncated peptides that were derived from the motif. Alignment and comparison of the 15E2-defined epitope sequence with the sequences of other coronaviruses indicated that the epitope is well conserved among chicken and turkey coronaviruses. The identified epitope should be useful in clinical applications and as a tool for the further study of the structure and function of the M protein of IBV.
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- A Candidate Antigen of the Recombinant Membrane Protein Derived from the Porcine Deltacoronavirus Synthetic Gene to Detect Seropositive Pigs
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Viruses.2023; 15(5): 1049. CrossRef - Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potential
Alan Rodríguez-Enríquez, Irma Herrera-Camacho, Lourdes Millán-Pérez-Peña, Julio Reyes-Leyva, Gerardo Santos-López, José Francisco Rivera-Benítez, Nora Hilda Rosas-Murrieta, Claude Krummenacher
PLOS ONE.2022; 17(2): e0263582. CrossRef - Genetic and antigenic heterogeneity of infectious bronchitis virus in South America: implications for control programmes
Ana Marandino, Ariel Vagnozzi, María Isabel Craig, Gonzalo Tomás, Claudia Techera, Yanina Panzera, Federico Vera, Ruben Pérez
Avian Pathology.2019; 48(3): 270. CrossRef - Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken
Lei Tan, Ying Liao, Jin Fan, Yuqiang Zhang, Xiang Mao, Yingjie Sun, Cuiping Song, Xusheng Qiu, Chunchun Meng, Chan Ding
Vaccine.2016; 34(3): 380. CrossRef - Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection
Lei Tan, Yuqiang Zhang, Fang Liu, Yanmei Yuan, Yuan Zhan, Yingjie Sun, Xusheng Qiu, Chunchun Meng, Cuiping Song, Chan Ding
Vaccine.2016; 34(44): 5209. CrossRef - Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein
Hui Dong, Xin Zhang, Hongyan Shi, Jianfei Chen, Da Shi, Yunnuan Zhu, Li Feng
Viruses.2016; 8(12): 327. CrossRef - Development of an ELISA based on a multi-fragment antigen of infectious bronchitis virus for antibodies detection
Meng-die Ding, Xin Yang, Hong-ning Wang, An-yun Zhang, Zhi-kun Zhang, Wen-qiao Fan, Hai-peng Cao
Biotechnology Letters.2015; 37(12): 2453. CrossRef - Two novel neutralizing antigenic epitopes of the s1 subunit protein of a QX-like avian infectious bronchitis virus strain Sczy3 as revealed using a phage display peptide library
Nianli Zou, Jing Xia, Fuyan Wang, Zhenzhen Duan, Dan Miao, Qigui Yan, Sanjie Cao, Xintian Wen, Ping Liu, Yong Huang
Veterinary Immunology and Immunopathology.2015; 168(1-2): 49. CrossRef - Adaptation and Attenuation of Duck Tembusu Virus Strain Du/CH/LSD/110128 following Serial Passage in Chicken Embryos
Ling Sun, Yunxia Li, Yue Zhang, Zongxi Han, Yang Xu, Xiangang Kong, Shengwang Liu, R. L. Hodinka
Clinical and Vaccine Immunology.2014; 21(8): 1046. CrossRef - Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus
Zhibang Zhang, Jianfei Chen, Hongyan Shi, Xiaojin Chen, Da Shi, Li Feng, Bin Yang
Virology Journal.2012;[Epub] CrossRef - Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus
Xiaoli Liu, Zongxi Han, Yuhao Shao, Dan Yu, Huixin Li, Yu Wang, Xiangang Kong, Shengwang Liu
Virology Journal.2010;[Epub] CrossRef
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