Journal of Microbiology

Article

Furan-based Chalcone Annihilates the Multi-Drug-Resistant Pseudomonas aeruginosa and Protects Zebra Fish against its Infection: Santosh Pushpa Ramya Ranjan Nayak , Catharine Basty , Seenivasan Boopathi , Loganathan Sumathi Dhivya , Khaloud Mohammed Alarjani , Mohamed Ragab Abdel Gawwad , Raghda Hager , Muthu Kumaradoss Kathiravan , Jesu Arockiaraj; J. Microbiol. 2024;62(2):75-89. Published online February 21, 2024; DOI: https://doi.org/10.1007/s12275-024-00103-6

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The emergence of carbapenem-resistant Pseudomonas aeruginosa, a multi-drug-resistant bacteria, is becoming a serious public health concern. This bacterium infects immunocompromised patients and has a high fatality rate. Both naturally and synthetically produced chalcones are known to have a wide array of biological activities. The antibacterial properties of synthetically produced chalcone were studied against P. aeruginosa. In vitro, study of the compound (chalcone derivative named DKO1), also known as (2E)-1-(5-methylfuran-2-yl)-3-(4-nitrophenyl) prop-2-en-1-one, had substantial antibacterial and biofilm disruptive action. DKO1 effectively shielded against P. aeruginosa-induced inflammation, oxidative stress, lipid peroxidation, and apoptosis in zebrafish larvae. In adult zebrafish, the treatment enhanced the chances of survivability and reduced the sickness-like behaviors. Gene expression, biochemical analysis, and histopathology studies found that proinflammatory cytokines (TNF-α, IL-1β, IL-6, iNOS) were down regulated; antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) levels increased, and histoarchitecture was restored in zebrafish. The data indicate that DKO1 is an effective antibacterial agent against P. aeruginosa demonstrated both in vitro and in vivo.

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Journal of Hazardous Materials.2026; 501: 140692. CrossRef
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Karthikeyan Ramamurthy, S. P. Ramya Ranjan Nayak, S. Madesh, Siva Prasad Panda, K. Manikandan, Rajakrishnan Rajagopal, Ahmed Alfarhan, Senthilkumar Palaniappan, Ajay Guru, M. K. Kathiravan, Jesu Arockiaraj
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Review

Current status and perspectives on vaccine development against dengue virus infection: Jisang Park , Ju Kim , Yong-Suk Jang; J. Microbiol. 2022;60(3):247-254. Published online February 14, 2022; DOI: https://doi.org/10.1007/s12275-022-1625-y

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Abstract PDF

Dengue virus (DENV) consists of four serotypes in the family Flaviviridae and is a causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. DENV is transmitted by mosquitoes, Aedes aegypti and A. albopictus, and is mainly observed in areas where vector mosquitoes live. The number of dengue cases reported by the World Health Organization increased more than 8-fold over the last two decades from 505,430 in 2000 to over 2.4 million in 2010 to 5.2 million in 2019. Although vaccine is the most effective
method
against DENV, only one commercialized vaccine exists, and it cannot be administered to children under 9 years of age. Currently, many researchers are working to resolve the various problems hindering the development of effective dengue vaccines; understanding of the viral antigen configuration would provide insight into the development of effective vaccines against DENV infection. In this review, the current status and perspectives on effective vaccine development for DENV are examined. In addition, a plausible direction for effective vaccine development against DENV is suggested.

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Efficacy of Multivalent Dengue Vaccine Candidates Predicted In Silico
Seokhwan Hyeon, Kwangwook Kim, Yoo Jin Na, Mihee Kim, Jaenam Jeong, Byung Chul Kim, Yookyoung Lee
Vaccines.2026; 14(2): 114. CrossRef
Identification of Natural Product-Based Inhibitors for DENV NS2B/NS3 Protease and RNA-Dependent RNA Polymerase Through Molecular Modelling and Simulation
Aishwarya Khare, Varsha Tripathi, Vivek Dhar Dwivedi, Neelam Pathak, Shivi Srivastava
Journal of Pharmaceutical Innovation.2026;[Epub] CrossRef
Stochastic Runge–Kutta for numerical treatment of dengue epidemic model with Brownian uncertainty
Nabeela Anwar, Iftikhar Ahmad, Hijab Javaid, Adiqa Kausar Kiani, Muhammad Shoaib, Muhammad Asif Zahoor Raja
Modern Physics Letters B.2025;[Epub] CrossRef
Dengue: epidemiology, diagnosis methods, treatment options, and prevention strategies
Dimple Kothari, Niralee Patel, Ashok Kumar Bishoyi
Archives of Virology.2025;[Epub] CrossRef
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Ruchi Jaiswal, Pramod Kumar Kushawaha
The Microbe.2025; 7: 100387. CrossRef
Análisis de la efectividad del control por insecticida para el dengue: una perspectiva matemática
Luis Eduardo López Montenegro
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Jirayu Boonyakida, Mami Matsuda, Ryosuke Suzuki, Krishna Raja Muthuraman, Enoch Y. Park
Biomacromolecules.2025; 26(7): 4449. CrossRef
Mature dengue virus particles inactivated by a non-ionic detergent retain quaternary neutralizing epitopes and induce potent neutralizing antibodies
Wen-Yang Tsai, Theodore C. Pierson, Jens Wrammert, Wanwisa Dejnirattisai, Amonrat Jumnainsong, Juthathip Mongkolsapaya, Gavin Screaton, James E. Crowe, Wei-Kung Wang
Frontiers in Immunology.2025;[Epub] CrossRef
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Pamela Ruvalcaba-Hernández, María del Rocío Thompson-Bonilla, Diana Casique-Aguirre, José Eugenio Vázquez-Meraz, Elva Jiménez-Hernández, Juan Manuel Mejía-Aranguré, Silvia Melchor-Doncel de la Torre, Angélica Rangel-López, Juan Xicohtencatl-Cortes, Sara A
Natural Product Communications.2025;[Epub] CrossRef
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Luciana de Souza Fernandes, Carine Ribeiro Pessoa, Roberto Sousa Dias, Cynthia Canedo da Silva, Sérgio Oliveira de Paula
Vaccine: X.2025; 26: 100705. CrossRef
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Biotechnology Reports.2024; 42: e00844. CrossRef
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Journal of Proteins and Proteomics.2024; 15(4): 675. CrossRef
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Noura M. Alshiban, Munirah S. Aleyiydi, Majed S. Nassar, Nada K. Alhumaid, Thamer A. Almangour, Yahya M.K. Tawfik, Laila A. Damiati, Abdulaziz S. Almutairi, Essam A. Tawfik
Saudi Pharmaceutical Journal.2024; 32(7): 102126. CrossRef
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Indian Journal of Community Medicine.2024; 49(2): 249. CrossRef
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Zahra Heydarifard, Fatemeh Heydarifard, Fatemeh Sadat Mousavi, Milad Zandi
Frontiers in Public Health.2024;[Epub] CrossRef
All-Atom Perspective of the DENV-3 Methyltransferase Inhibition Mechanism
Xiao Liu, Kaiwen Pang, Hangfei Wu, Xiaohui Wang, John Z. H. Zhang, Zhaoxi Sun
The Journal of Physical Chemistry B.2024; 128(50): 12358. CrossRef
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Keyi Yu, Jianping Wu, Minghao Wang, Yizhou Cai, Minhui Zhu, Shenjun Yao, Yibin Zhou
Acta Tropica.2024; 255: 107234. CrossRef
Dengue
Gabriela Paz-Bailey, Laura E Adams, Jacqueline Deen, Kathryn B Anderson, Leah C Katzelnick
The Lancet.2024; 403(10427): 667. CrossRef
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Zubia Jamil, Samreen Khalid, Hafiz Muhammad Khan, Ikram Waheed, Amna Ehsan, Mohammed Alissa, Khalid Muhammad, Nayla Munawar, Yasir Waheed
Journal of Multidisciplinary Healthcare.2024; Volume 17: 2321. CrossRef
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Ying Nie, Dongqing Deng, Lumin Mou, Qizhou Long, Jinzhi Chen, Jiahong Wu
Journal of Microbiology and Biotechnology.2023; 33(5): 600. CrossRef
Deep learning approach for detection of Dengue fever from the microscopic images of blood smear
Hilda Mayrose, Niranjana Sampathila, G Muralidhar Bairy, Tushar Nayak, Sushma Belurkar, Kavitha Saravu
Journal of Physics: Conference Series.2023; 2571(1): 012005. CrossRef
Analysis of the correlation between climatic variables and Dengue cases in the city of Alagoinhas/BA
Marcos Batista Figueredo, Roberto Luiz Souza Monteiro, Alexandre do Nascimento Silva, José Roberto de Araújo Fontoura, Andreia Rita da Silva, Carolina Aparecida Pereira Alves
Scientific Reports.2023;[Epub] CrossRef
Dengue overview: An updated systemic review
Muhammad Bilal Khan, Zih-Syuan Yang, Chih-Yen Lin, Ming-Cheng Hsu, Aspiro Nayim Urbina, Wanchai Assavalapsakul, Wen-Hung Wang, Yen-Hsu Chen, Sheng-Fan Wang
Journal of Infection and Public Health.2023; 16(10): 1625. CrossRef
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Robert G.S. de Araújo, Daniel C.P. Jorge, Rejane C. Dorn, Gustavo Cruz-Pacheco, M. Lourdes M. Esteva, Suani T.R. Pinho
Mathematical Biosciences.2023; 360: 109013. CrossRef
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Fernando Amrein, Carolina Sarto, Leila A Cababie, F Luis Gonzalez Flecha, Sergio B Kaufman, Mehrnoosh Arrar
Nucleic Acids Research.2023; 51(20): 11213. CrossRef
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Analytical Methods.2023; 15(32): 3991. CrossRef
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Anon Thammasittirong, Sutticha Na-Ranong Thammasittirong
Toxins.2023; 15(2): 114. CrossRef
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Journal of Microbiology.2023; 61(1): 131. CrossRef
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Hanan S. Alyahya
Entomological Research.2023; 53(12): 539. CrossRef
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Food Reviews International.2023; 39(9): 6781. CrossRef
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Journal of Biomedical Science.2023;[Epub] CrossRef
Dengue hemorrhagic fever: a growing global menace
Shakeela Parveen, Zainab Riaz, Saba Saeed, Urwah Ishaque, Mehwish Sultana, Zunaira Faiz, Zainab Shafqat, Saman Shabbir, Sana Ashraf, Amna Marium
Journal of Water and Health.2023; 21(11): 1632. CrossRef
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Mamuna Mukhtar, Haris Ahmed Khan, Najam us Sahar Sadaf Zaidi
RSC Advances.2023; 13(27): 18306. CrossRef
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Viruses.2022; 15(1): 52. CrossRef
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Asian Pacific Journal of Tropical Medicine.2022; 15(11): 496. CrossRef
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International Journal of Molecular Sciences.2022; 23(22): 13911. CrossRef

Article

Potent antibacterial and antibiofilm activities of TICbf-14, a peptide with increased stability against trypsin: Liping Wang , Xiaoyun Liu , Xinyue Ye , Chenyu Zhou , Wenxuan Zhao , Changlin Zhou , Lingman Ma; J. Microbiol. 2022;60(1):89-99. Published online December 29, 2021; DOI: https://doi.org/10.1007/s12275-022-1368-9

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Abstract PDF

The poor stability of peptides against trypsin largely limits their development as potential antibacterial agents. Here, to obtain a peptide with increased trypsin stability and potent antibacterial activity, TICbf-14 derived from the cationic peptide Cbf-14 was designed by the addition of disulfide-bridged hendecapeptide (CWTKSIPPKPC) loop. Subsequently, the trypsin stability and antimicrobial and antibiofilm activities of this peptide were evaluated. The possible mechanisms underlying its mode of action were also clarified. The results showed that TICbf-14 exhibited elevated trypsin inhibitory activity and effectively mitigated lung histopathological damage in bacteria-infected mice by reducing the bacterial counts, further inhibiting the systemic dissemination of bacteria and host inflammation. Additionally, TICbf-14 significantly repressed bacterial swimming motility and notably inhibited biofilm formation. Considering the mode of action, we observed that TICbf-14 exhibited a potent membrane-disruptive mechanism, which was attributable to its destructive effect on ionic bridges between divalent cations and LPS of the bacterial membrane. Overall, TICbf-14, a bifunctional peptide with both antimicrobial and trypsin inhibitory activity, is highly likely to become an ideal candidate for drug development against bacteria.

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Dandan Feng, Yakun Song, Zuqi Wu, Wuruo Liu, Yuting Pu, Yangcan Gao, Yuying Sun, Jiquan Zhang, Tzong-Yueh Chen
PLOS One.2026; 21(2): e0342746. CrossRef
Modified polymeric biomaterials with antimicrobial and immunomodulating properties
Katarzyna Szałapata, Mateusz Pięt, Martyna Kasela, Marcin Grąz, Justyna Kapral-Piotrowska, Aleksandra Mordzińska-Rak, Elżbieta Samorek, Paulina Pieniądz, Jolanta Polak, Monika Osińska-Jaroszuk, Roman Paduch, Bożena Pawlikowska-Pawlęga, Anna Malm, Anna Jar
Scientific Reports.2024;[Epub] CrossRef
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Li Fan, Yingying Wei, Yi Chen, Shu Jiang, Feng Xu, Chundan Zhang, Hongfei Wang, Xingfeng Shao
Food Chemistry.2023; 403: 134419. CrossRef

Reviews

Importance of differential identification of Mycobacterium tuberculosis strains for understanding differences in their prevalence, treatment efficacy, and vaccine development: Hansong Chae , Sung Jae Shin; J. Microbiol. 2018;56(5):300-311. Published online May 2, 2018; DOI: https://doi.org/10.1007/s12275-018-8041-3

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Abstract PDF

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a serious global health problem in the 21st century because of its high mortality. Mtb is an extremely successful human-adapted pathogen that displays a multifactorial ability to control the host immune response and to evade killing by drugs, resulting in the breakdown of BCG vaccine-conferred anti-TB immunity and development of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mtb. Although genetic components of the genomes of the Mtb complex strains are highly conserved, showing over 99% similarity to other bacterial genera, recently accumulated evidence suggests that the genetic diversity of the Mtb complex strains has implications for treatment outcomes, development of MDR/XDR Mtb, BCG vaccine efficacy, transmissibility, and epidemiological outbreaks. Thus, new insights into the pathophysiological features of the Mtb complex strains are required for development of novel vaccines and for control of MDR/XDR Mtb infection, eventually leading to refinement of treatment regimens and the health care system. Many studies have focused on the differential identification of Mtb complex strains belonging to different lineages because of differences in their virulence and geographical dominance. In this review, we discuss the impact of differing genetic characteristics among Mtb complex strains on vaccine efficacy, treatment outcome, development of MDR/ XDR Mtb strains, and epidemiological outbreaks by focusing on the best-adapted human Mtb lineages. We further explore the rationale for differential identification of Mtb strains for more effective control of TB in clinical and laboratory settings by scrutinizing current diagnostic methods.

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Tesfaye Gebreyohannis Hailemariam, Melaku Tilahun, Abay Atnafu, Tesfaye Gelanew, Tewodros Tariku Gebresilase, Mekdes Alemu Tola, Abaysew Ayele, Shewki Moga Siraj, Workineh Shibeshi, Kidist Bobosha, Liya Wassie, Yonas Hirutu, Ephrem Engidawork
Frontiers in Microbiology.2025;[Epub] CrossRef
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Sudhir Kumar Prajapati, Deepti Narang, Mudit Chandra, Ashwani Sharma, Sikh Tejinder Singh
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Ren Liu, Tianhuan Song, Xiaolin Chen, Zhiheng Yu, Cunliang Yang, Xianchao Zhang, Fengli Huang, Hao Jia, Jijun Feng, Yujiong Wang
Biotechnology and Bioengineering.2025; 122(4): 1025. CrossRef
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Haiyang Zhang, Yi Liao, Zhensheng Zhu, Hanmin Liu, Deyuan Li, Sisi Wang
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
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Haiyang Zhang, Maoting Tang, Deyuan Li, Min Xu, Yusen Ao, Liangkang Lin
Frontiers in Public Health.2024;[Epub] CrossRef
Distinct contributions of the innate immune receptors TLR2 and RP105 to formation and architecture of structured lung granulomas in mice infected with Mycobacterium tuberculosis
Meg L. Donovan, Helle Bielefeldt‐Ohmann, Rachel F. Rollo, Stephen J. McPherson, Thomas E. Schultz, Giorgia Mori, Jessica C. Kling, Antje Blumenthal
Immunology.2023; 169(1): 13. CrossRef
Host vs. pathogen evolutionary arms race: Effects of exposure history on individual response to a genetically diverse pathogen
Daniel P. Walsh, Brandi L. Felts, E. Frances Cassirer, Thomas E. Besser, Jonathan A. Jenks
Frontiers in Ecology and Evolution.2023;[Epub] CrossRef
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Carmen Molina-Torres, Carlos Pedraza-Rodríguez, Lucio Vera-Cabrera, Jorge Ocampo-Candiani, Catalina Rivas-Morales, Ezequiel Viveros-Valdez
Antibiotics.2023; 12(5): 833. CrossRef
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Anabel Ordaz-Vázquez, Pedro Torres-González, Leticia Ferreyra-Reyes, Sergio Canizales-Quintero, Guadalupe Delgado-Sánchez, Lourdes García-García, Alfredo Ponce-De-León, José Sifuentes-Osornio, Miriam Bobadilla-Del-Valle
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Will we ever eradicate animal tuberculosis?
Christian Gortázar, José de la Fuente, Alberto Perelló, Lucas Domínguez
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Junaid Shaik, Manormoney Pillay, Julie Moodley, Prakash Jeena
Tuberculosis.2022; 136: 102250. CrossRef
Dysglycemia is associated with Mycobacterium tuberculosis lineages in tuberculosis patients of North Lima—Peru
Kattya Lopez, María B. Arriaga, Juan G. Aliaga, Nadia N. Barreda, Oswaldo M. Sanabria, Chuan-Chin Huang, Zibiao Zhang, Ruth García-de-la-Guarda, Leonid Lecca, Anna Cristina Calçada Carvalho, Afrânio L. Kritski, Roger I. Calderon, Igor Mokrousov
PLOS ONE.2021; 16(1): e0243184. CrossRef
In vitro Synergism of Six Antituberculosis Agents Against Drug-Resistant Mycobacterium tuberculosis Isolated from Retreatment Tuberculosis Patients
Ruoyan Ying, Xiaochen Huang, Yaxian Gao, Jie Wang, Yidian Liu, Wei Sha, Hua Yang
Infection and Drug Resistance.2021; Volume 14: 3729. CrossRef
Characterisation of secretome-based immune responses of human leukocytes infected with variousMycobacterium tuberculosislineages
Benjawan Kaewseekhao, Sittiruk Roytrakul, Yodying Yingchutrakul, Marut Laohaviroj, Kanin Salao, Kiatichai Faksri
PeerJ.2021; 9: e11565. CrossRef
Different PPD-stimulated cytokine responses from patients infected with genetically distinct Mycobacterium tuberculosis complex lineages
Paulo Ranaivomanana, Marie Sylvianne Rabodoarivelo, Mame Diarra Bousso Ndiaye, Niaina Rakotosamimanana, Voahangy Rasolofo
International Journal of Infectious Diseases.2021; 104: 725. CrossRef
A review of published spoligotype data indicates the diversity of Mycobacterium tuberculosis from India is under-represented in global databases
Husain Poonawala, Narender Kumar, Sharon J. Peacock
Infection, Genetics and Evolution.2020; 78: 104072. CrossRef
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Kh. Ansarin, L. Sahebi, Y. Aftabi, M. Khalili, M. Seyyedi
Journal of Applied Microbiology.2020; 129(4): 1062. CrossRef
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Bahram Golestani Eimani, Khalil Ansarin, Leila Sahebi, Maryam Seyyedi
Iranian South Medical Journal.2020; 23(2): 87. CrossRef
Comparison of the Three Molecular Diagnostic Assays for Molecular Identification ofMycobacterium tuberculosisand Nontuberculous Mycobacteria Species in Sputum Samples
Jinyoung Bae, Sung-Bae Park, Ji-Hoi Kim, Mi Ran Kang, Kyung Eun Lee, Sunghyun Kim, Hyunwoo Jin
Biomedical Science Letters.2020; 26(3): 170. CrossRef
Immunogenicity and Vaccine Potential of InsB, an ESAT-6-Like Antigen Identified in the Highly Virulent Mycobacterium tuberculosis Beijing K Strain
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Mycobacterium tuberculosis is the causative agent of tuberculosis (TB), a major health issue of the present era. The bacterium inhabits the host macrophage and other immune cells where it modulates the lysosome trafficking protein, hinders the formation of phagolysosome, and blocks the TNF receptor- dependent apoptosis of host macrophage/monocytes. Other limitations such as resistance to and low bioavailability and bio-distribution of conventional drugs aid to their high virulence and human mortality. This review highlights the use of nanotechnology-based approaches for drug formulation and delivery which could open new avenues to limit the pathogenicity of tuberculosis. Moreover phytochemicals, such as alkaloids, phenols, saponins, steroids, tannins, and terpenoids, extracted from terrestrial plants and mangroves seem promising against M. tuberculosis through different molecular mechanisms. Further understanding of the genomics and proteomics of this pathogenic microbe could also help overcome various research gaps in the path of developing a suitable therapy against tuberculosis.

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This study aimed to provide information that bedaquilline is significantly effective for treatment of totally drug resistant (TDR) Mycobacterium tuberculosis that shows resistant to all first- and second-line drugs-using an innovative disc agarose channel (DAC) system. Time-lapse images of single bacterial cells under culture conditions with different concentrations of bedaquiline were analysed by image processing software to determine minimum inhibitory concentrations (MICs). Bedaquiline inhibited the growth of TDR M. tuberculosis strains, with MIC values ranging from 0.125 to 0.5 mg/L. The results of the present study demonstrate that bedaquiline, newly approved by the United States Food and Drug Admi-nistration (FDA), may offer therapeutic solutions for TDR -TB.

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The type VI secretion system (T6SS) is a widespread and versatile protein secretion system found in most Gram- negative bacteria. Studies of T6SS have mainly focused on its role in virulence toward host cells and inter-bacterial inter-actions, but studies have also shown that T6SS4 in Yersinia pseudotuberculosis participates in the acquisition of zinc ions to alleviate the accumulation of hydroxyl radicals induced by multiple stressors. Here, by comparing the gene expression patterns of wild-type and zntR mutant Y. pseudotubercu-losis cells using RNA-seq analysis, T6SS4 and 17 other bio-logical processes were found to be regulated by ZntR. T6SS4 was positively regulated by ZntR in Y. pseudotuberculosis, and further investigation demonstrated that ZntR regulates T6SS4 by directly binding to its promoter region. T6SS4 ex-pression is regulated by zinc via ZntR, which maintains in-tracellular zinc homeostasis and controls the concentration of reactive oxygen species to prevent bacterial death under oxidative stress. This study provides new insights into the regulation of T6SS4 by a zinc-dependent transcriptional regu-lator, and it provides a foundation for further investigation of the mechanism of zinc transport by T6SS.

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Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis: Pallavi Sinha , Pradyot Prakash , Shashikant C.U. Patne , Shampa Anupurba , Sweety Gupta , G. N. Srivastava; J. Microbiol. 2017;55(1):63-67. Published online December 30, 2016; DOI: https://doi.org/10.1007/s12275-017-6127-y

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The conventional methods for diagnosis of tubercular lymphadenitis (TBLN) such as - fine needle aspiration cytology, Ziehl-Neelsen staining and culture have limitations of low sensitivity and/or specificity. So, it becomes essential to develop a rapid, sensitive, and specific method for an early diagnosis of TBLN. Therefore, the present study was conducted to evaluate nested multiplex polymerase chain reaction (nMPCR) targeting MTP40 and IS6110 gene sequences of Mycobacterium tuberculosis and Mycobacterium tuberculosis complex, respectively in 48 successive patients of TBLN and 20 random patients with non-tubercular lymph node lesions. Out of the 48 cases of TBLN, 14 (29.2%) were found to be positive by Ziehl-Neelsen staining, 15 (31.2%) were positive by culture and 43 (89.6%) cases were positive after first round of PCR while 48 (100%) cases were positive by nMPCR assay. The sensitivity and specificity of nMPCR was found to be 100% for the diagnosis of TBLN. The results thus obtained indicate that nMPCR assay is a highly sensitive and specific tool for the diagnosis of TBLN.

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Pulmonary tuberculosis (TB) is caused by Mycobacterium tuberculosis. The protein composition of sputum may reflect the immune status of the lung. This study aimed to evaluate the protein profiles in spontaneous sputum samples from patients with active pulmonary TB. Sputum samples were collected from patients with pulmonary TB and healthy controls. Western blotting was used to analyze the amount of interleukin 10 (IL-10), interferon-gamma (IFN-γ), IL-25, IL- 17, perforin-1, urease, albumin, transferrin, lactoferrin, adenosine deaminase (also known as adenosine aminohydrolase, or ADA), ADA-2, granzyme B, granulysin, and caspase- 1 in sputum. Results of detection of IL-10, IFN-γ, perforin- 1, urease, ADA2, and caspase-1, showed relatively high specificity in distinguishing patients with TB from healthy controls, although sensitivities varied from 13.3% to 66.1%. By defining a positive result as the detection of any two proteins in sputum samples, combined use of transferrin and urease as markers increased sensitivity to 73.2% and specificity to 71.1%. Furthermore, we observed that the concentration of transferrin was proportional to the number of acidfast bacilli detected in sputum specimens. Detection of sputum transferrin and urease was highly associated with pulmonary TB infection. In addition, a high concentration of transferrin detected in sputum might correlate with active TB infection. This data on sputum proteins in patients with TB may aid in the development of biomarkers to assess the severity of pulmonary TB.

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The physiology of dormant Mycobacterium tuberculosis was studied in detail by examining the gene expression of 51 genes using quantitative Reverse-Transcription Polymerase Chain Reaction. A forty-day period of dormancy in the Wayne culture model depicted four major transcription patterns. Some sigma factors and many metabolic genes were constant, whereas genes belonging to the dormancy regulon were activated on day 9. In particular, alpha-crystallin mRNA showed more than a 1,000-fold increase compared to replicating bacilli. Genes belonging to the enduring hypoxic response were up-regulated at day 16, notably, transcription factors sigma B and E. Early genes typical of log-phase bacilli, esat-6 and fbpB, were uniformly down-regulated during dormancy. Late stages of dormancy showed a drop in gene expression likely due to a lack of substrates in anaerobic respiration as demonstrated by the transcriptional activation observed following nitrates addition. Among genes involved in nitrate metabolism, narG was strongly up-regulated by nitrates addition. Dormant bacilli responded very rapidly when exposed to oxygen and fresh medium, showing a transcriptional activation of many genes, including resuscitation-promoting factors, within one hour. Our observations extend the current knowledge on dormant M. tuberculosis gene expression and its response to nutrients and to aerobic and anaerobic respiration.

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RJH Hammond, Frank Kloprogge, O. Della Pasqua, Stephen H. Gillespie
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International Journal of Peptide Research and Therapeutics.2020; 26(4): 2191. CrossRef
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Research Support, Non-U.S. Gov't

A small hairpin RNA targeting myeloid cell leukemia-1 enhances apoptosis in host macrophages infected with Mycobacterium tuberculosis: Fei-yu Wang , Yu-qing Zhang , Xin-min Wang , Chan Wang , Xiao-fang Wang , Jiang-dong Wu , Fang Wu , Wan-jiang Zhang , Le Zhang; J. Microbiol. 2016;54(4):330-337. Published online April 1, 2016; DOI: https://doi.org/10.1007/s12275-016-5627-5

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Abstract PDF

Myeloid cell leukemia-1 (Mcl-1) plays an important role in various cell survival pathways. Some studies indicated that the expression of Mcl-1 was upregulated in host cells during infection with the virulent Mycobacterium tuberculosis strain, H37Rv. The present study was designed to investigate the effect of inhibiting Mcl-1 expression both in vivo and in vitro on apoptosis of host macrophages infected with M. tuberculosis using a small hairpin (sh)RNA. Mcl-1 expression was detected by the real time-polymerase chain reaction, western blotting, and immunohistochemistry. Flow cytometry and transmission electron microscopy were used to measure host macrophage apoptosis. We found elevated Mcl-1 levels in host macrophages infected with M. tuberculosis H37Rv. The expression of Mcl-1 was downregulated efficiently in H37Rv-infected host macrophages using shRNA. Knockdown of Mcl-1 enhanced the extent of apoptosis in H37Rv-infected host macrophages significantly. The increased apoptosis correlated with a decrease in M. tuberculosis colony forming units recovered from H37Rv-infected cells that were treated with Mcl-1-shRNA. Reducing Mcl-1 accumulation by shRNA also reduced accumulation of the anti-apoptotic gene, Bcl-2, and increased expression of the pro-apoptotic gene, Bax, in H37Rv-infected host macrophages. Our results showed that specific knockdown of Mcl-1 expression increased apoptosis of host macrophages significantly and decreased the intracellular survival of a virulent strain of M. tuberculosis. These data indicate that interference with Mcl-1 expression may provide a new avenue for tuberculosis therapy.

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Ling Han, Yang Lu, Xiaofang Wang, Shujun Zhang, Yingzi Wang, Fang Wu, Wanjiang Zhang, Xinmin Wang, Le Zhang
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Current Opinion in Pharmacology.2019; 48: 33. CrossRef
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Research Support, N.I.H., Extramural

The Mycobacterium tuberculosis relBE toxin:antitoxin genes are stress-responsive modules that regulate growth through translation inhibition: Shaleen B. Korch , Vandana Malhotra , Heidi Contreras , Josephine E. Clark-Curtiss; J. Microbiol. 2015;53(11):783-795. Published online October 28, 2015; DOI: https://doi.org/10.1007/s12275-015-5333-8

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Abstract PDF

Toxin-antitoxin (TA) genes are ubiquitous among bacteria and are associated with persistence and dormancy. Following exposure to unfavorable environmental stimuli, several species (Escherichia coli, Staphylococcus aureus, Myxococcus xanthus) employ toxin proteins such as RelE and MazF to downregulate growth or initiate cell death. Mycobacterium tuberculosis possesses three Rel TA modules (RelMtb): RelBEMtb, RelFGMtb and RelJKMtb (Rv1246c-Rv1247c, Rv2865-Rv2866, and Rv3357-Rv3358, respectively), which inhibit mycobacterial growth when the toxin gene (relE, relG, relK) is expressed independently of the antitoxin gene (relB, relF, relJ). In the present study, we examined the in vivo mechanism of the RelEMtb toxin protein, the impact of RelEMtb on M. tuberculosis physiology and the environmental conditions that regulate all three relMtb modules. RelEMtb negatively impacts growth and the structural integrity of the mycobacterial envelope, generating cells with aberrant forms that are prone to extensive aggregation. At a time coincident with growth defects, RelEMtb mediates mRNA degradation in vivo resulting in significant changes to the proteome. We establish that relMtb modules are stress responsive, as all three operons are transcriptionally activated following mycobacterial exposure to oxidative stress or nitrogen-limiting growth environments. Here we present evidence that the relMtb toxin:antitoxin family is stress-responsive and, through the degradation of mRNA, the RelEMtb toxin influences the growth, proteome and morphology of mycobacterial cells.

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Shafinaz Rahman Sarah, Nimisha Sinha, Harsh A. Gandhi, Jaydeep Bhattacharya, Vandana Malhotra
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research.2026; 1873(2): 120097. CrossRef
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Aayush Bahl, Roopshali Rakshit, Saurabh Pandey, Deeksha Tripathi
Biotechnology and Applied Biochemistry.2025; 72(1): 116. CrossRef
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Maryam Shafipour, Abdolmajid Mohammadzadeh, Ezzat Allah Ghaemi, Pezhman Mahmoodi, Nader Mosavari
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Johana E. Hernández Toloza, Ye Xu, Tom A. Mendum, Bianca Sica Siedler, Rosalyn Casey, Huihai Wu, Kerstin Williams, Suzanne Hingley-Wilson, Johnjoe McFadden
Scientific Reports.2025;[Epub] CrossRef
Resilience to stress and antibiotics, coupled with immunomodulatory behavior, uncovers Mycobacterium indicus pranii as a suitable surrogate model for tuberculosis research
Aayush Bahl, Khushboo Negi, Anupam Anupam, Simran Choudhary, Sashi Kant, Saurabh Pandey, Deeksha Tripathi
Biochemical and Biophysical Research Communications.2025; 777: 152296. CrossRef
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Nanna Boll Greve, Xiao Fei, Anders Per Håkansson, John Elmerdahl Olsen, Line Elnif Thomsen
Scientific Reports.2025;[Epub] CrossRef
Bacterial persisters: molecular mechanisms and therapeutic development
Hongxia Niu, Jiaying Gu, Ying Zhang
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Yiduo Liu, Han Li, Dejia Dai, Jiakang He, Zhengmin Liang
Current Issues in Molecular Biology.2024; 46(6): 5825. CrossRef
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Zian Zhang, Lifa Xu, Xiaochun Wang, LingYun Kong, Zilun Shi, Qiangsen Zhong, Yun Xu, Jianghong Wang
Tuberculosis.2024; 145: 102480. CrossRef
Enhancement of mycobacterial pathogenesis by host interferon-γ
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Maryam Shafipour, Abdolmajid Mohammadzadeh, Pezhman Mahmoodi, Mahdi Dehghanpour, Ezzat Allah Ghaemi, Francesca Boldrin
PLOS ONE.2024; 19(10): e0309292. CrossRef
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Anna Kopf, Boyke Bunk, Thomas Riedel, Percy Schröttner
BMC Microbiology.2024;[Epub] CrossRef
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Maryam Shafipour, Abdolmajid Mohammadzadeh, Ezzat Allah Ghaemi, Pezhman Mahmoodi
Current Microbiology.2024;[Epub] CrossRef
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Satakshi Hazra, Risha Hazarika, Sanjukta Patra
Current Drug Targets.2023; 24(9): 751. CrossRef
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Kanglun Jiang, Tan Wang, Zirui Zhao, Lei Zhou, Huaili Jiang, Na Shen, Daquan Wu, Xinsheng Huang
Journal of Biomedical Nanotechnology.2023; 19(4): 600. CrossRef
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Kefan BI, Dan CAO, Cheng DING, Shuihua LU, Hongzhou LU, Guangyu ZHANG, Wenhong ZHANG, Liang LI, Kaijin XU, Lanjuan LI, Ying ZHANG
Journal of Zhejiang University (Medical Sciences).2022; 51(6): 657. CrossRef
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Mazen E. Aljghami, Marim M. Barghash, Emily Majaesic, Vaibhav Bhandari, Walid A. Houry
Frontiers in Molecular Biosciences.2022;[Epub] CrossRef
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Vandana Malhotra, Blessing P. Okon, Akash T. Satsangi, Sumana Das, Uchenna Watson Waturuocha, Atul Vashist, Josephine E. Clark-Curtiss, Deepak Kumar Saini, Amit Singh
Microbiology Spectrum.2022;[Epub] CrossRef
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Martin Ziegler, Julia Zieringer, Ralf Takors
Microbial Biotechnology.2021; 14(3): 993. CrossRef
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Laura Veschetti, Angela Sandri, Cristina Patuzzo, Paola Melotti, Giovanni Malerba, Maria M. Lleò
Microorganisms.2021; 9(1): 130. CrossRef
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Michal Ziemski, Julia Leodolter, Gabrielle Taylor, Anne Kerschenmeyer, Eilika Weber‐Ban
The FEBS Journal.2021; 288(1): 99. CrossRef
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Chih-Han Tu, Michelle Holt, Shengfeng Ruan, Christina Bourne
Toxins.2020; 12(6): 422. CrossRef
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Marcin Równicki, Robert Lasek, Joanna Trylska, Dariusz Bartosik
Toxins.2020; 12(9): 568. CrossRef
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Biocatalysis and Agricultural Biotechnology.2019; 22: 101401. CrossRef
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Lauren R. Walling, J. Scott Butler
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J S Solano-Gutierrez, C Pino, J Robledo
FEMS Microbiology Letters.2019;[Epub] CrossRef
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Wing W. Yew, Takashi Yoshiyama, Chi C. Leung, Denise P. Chan
Respirology.2018; 23(6): 567. CrossRef
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Wing Wai Yew, Denise P Chan, Chi Chiu Leung, Ying Zhang
American Journal of Epidemiology.2018; 187(7): 1570. CrossRef
System-Wide Analysis Unravels the Differential Regulation and In Vivo Essentiality of Virulence-Associated Proteins B and C Toxin-Antitoxin Systems of Mycobacterium tuberculosis
Sakshi Agarwal, Prabhakar Tiwari, Amar Deep, Saqib Kidwai, Shamba Gupta, Krishan Gopal Thakur, Ramandeep Singh
The Journal of Infectious Diseases.2018; 217(11): 1809. CrossRef
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Journal of Antimicrobial Chemotherapy.2018; 73(5): 1117. CrossRef
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Journal of Drug Targeting.2017; 25(4): 296. CrossRef
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Microbiology Spectrum.2016;[Epub] CrossRef
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Research Support, Non-U.S. Gov'ts

Roles of RpoS in Yersinia pseudotuberculosis stress survival, motility, biofilm formation and type VI secretion system expression: Jingyuan Guan , Xiao Xiao , Shengjuan Xu , Fen Gao , Jianbo Wang , Tietao Wang , Yunhong Song , Junfeng Pan , Xihui Shen , Yao Wang; J. Microbiol. 2015;53(9):633-642. Published online August 27, 2015; DOI: https://doi.org/10.1007/s12275-015-0099-6

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Abstract PDF

RpoS (σS), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. However, the role of RpoS appears to differ in different bacteria. While RpoS is an important regulator of flagellum biosynthesis, it is associated with biofilm development in Edwardsiella tarda. Biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. The type VI secretion system (T6SS) discovered recently is reportedly associated with several phenotypes, ranging from biofilm formation to stress sensing. For example, Vibrio anguillarum T6SS was proposed to serve as a sensor for extracytoplasmic signals and modulates RpoS expression and stress response. In this study, we investigated the physiological roles of RpoS in Yersinia pseudotuberculosis, including bacterial survival under stress conditions, flagella formation, biofilm development and T6SS expression. We found that RpoS is important in resistance to multiple stressors–including H2O2, acid, osmotic and heat shock–in Y. pseudotuberculosis. In addition, our study showed that RpoS not only modulates the expression of T6SS but also regulates flagellum formation by positively controlling the flagellar master regulatory gene flhDC, and affects the formation of biofilm on Caenorhabditis elegans by regulating the synthesis of exopolysaccharides. Taken together, these results show that RpoS plays a central role in cell fitness under several adverse conditions in Y. pseudotuberculosis.

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International Journal of Molecular Sciences.2023; 24(24): 17348. CrossRef
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Performance of a real-time PCR assay for the rapid identification of Mycobacterium species: Hye-young Wang , Hyunjung Kim , Sunghyun Kim , Do-kyoon Kim , Sang-Nae Cho , Hyeyoung Lee; J. Microbiol. 2015;53(1):38-46. Published online January 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4495-8

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Mycobacteria cause a variety of illnesses that differ in severity and public health implications. The differentiation of Mycobacterium tuberculosis (MTB) from nontuberculous mycobacteria (NTM) is of primary importance for infection control and choice of antimicrobial therapy. The diagnosis of diseases caused by NTM is difficult because NTM species are prevalent in the environment and because they have fastidious properties. In the present study, we evaluated 279 clinical isolates grown in liquid culture provided by The Catholic University of Korea, St. Vincent’s Hospital using real-time PCR based on mycobacterial rpoB gene sequences. The positive rate of real-time PCR assay accurately discriminated 100% (195/195) and 100% (84/84) between MTB and NTM species. Comparison of isolates identified using the MolecuTech REBA Myco-ID? and Real Myco-ID? were completely concordant except for two samples. Two cases that were identified as mixed infection (M. intracellulare-M. massiliense and M. avium-M. massiliense co-infection) by PCRREBA assay were only detected using M. abscessus-specific probes by Real Myco-ID?. Among a total of 84 cases, the most frequently identified NTM species were M. intracellulare (n=38, 45.2%), M. avium (n=18, 23.7%), M. massiliense (n=10, 13.2%), M. fortuitum (n=5, 6%), M. abscessus (n=3, 3.9%), M. gordonae (n=3, 3.9%), M. kansasii (n=2, 2.4%), M. mucogenicum (n=2, 2.4%), and M. chelonae (n= 1, 1.2%). Real Myco-ID? is an efficient tool for the rapid detection of NTM species as well as MTB and sensitive and specific and comparable to conventional methods.

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Phosphorylation Regulates Mycobacterial Proteasome: Tripti Anandan , Jaeil Han , Heather Baun , Seeta Nyayapathy , Jacob T. Brown , Rebekah L. Dial , Juan A. Moltalvo , Min-Seon Kim , Seung Hwan Yang , Donald R. Ronning , Robert N. Husson , Joowon Suh , Choong-Min Kang; J. Microbiol. 2014;52(9):743-754. Published online September 2, 2014; DOI: https://doi.org/10.1007/s12275-014-4416-2

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Mycobacterium tuberculosis possesses a proteasome system that is required for the microbe to resist elimination by the host immune system. Despite the importance of the proteasome in the pathogenesis of tuberculosis, the molecular mechanisms by which proteasome activity is controlled remain largely unknown. Here, we demonstrate that the α-subunit (PrcA) of the M. tuberculosis proteasome is phosphorylated by the PknB kinase at three threonine residues (T84, T202, and T178) in a sequential manner. Furthermore, the proteasome with phosphorylated PrcA enhances the degradation of Ino1, a known proteasomal substrate, suggesting that PknB regulates the proteolytic activity of the proteasome. Previous studies showed that depletion of the proteasome and the proteasome- associated proteins decreases resistance to reactive nitrogen intermediates (RNIs) but increases resistance to hydrogen peroxide (H2O2). Here we show that PknA phosphorylation of unprocessed proteasome β-subunit (pre-PrcB) and α-subunit reduces the assembly of the proteasome complex and thereby enhances the mycobacterial resistance to H2O2 and that H2O2 stress diminishes the formation of the proteasome complex in a PknA-dependent manner. These findings indicate that phosphorylation of the M. tuberculosis proteasome not only modulates proteolytic activity of the proteasome, but also affects the proteasome complex formation contributing to the survival of M. tuberculosis under oxidative stress conditions.

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Characterization of a Novel Antigen of Mycobacterium tuberculosis K Strain and Its Use in Immunodiagnosis of Tuberculosis: Paul J. Park , Ah Reum Kim , Yangkyo P. Salch , Taeksun Song , Sung Jae Shin , Seung Jung Han , Sang-Nae Cho; J. Microbiol. 2014;52(10):871-878. Published online August 27, 2014; DOI: https://doi.org/10.1007/s12275-014-4235-5

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*For correspondence. (S.J. Han) E-mail: hansjung@yuhs.ac / (S.N. Cho) E-mail: raycho@yuhs.ac Paul J. Park, Ah Reum Kim, Yangkyo P. Salch, Taeksun Song, Sung Jae Shin, Seung Jung Han*, and Sang-Nae Cho* Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea (Received Apr 16, 2014 / Revised Jul 14, 2014 / Accepted Jul 16, 2014) Journal of Microbiology (2014) Vol. 52, No. 10, pp. 871–878 Copyright 􎨰􀁇2014, The Microbiological Society of Korea DOI 10.1007/s12275-014-4235-5 Characterization of a Novel Antigen of Mycobacterium tuberculosis K strain and Its Use in Immunodiagnosis of Tuberculosis Mycobacterium tuberculosis-specific antigens would be of great value in developing immunodiagnostic tests for tuberculosis (TB), but regional differences in molecular types of the organism may result in antigenic variation, which in turn affects the outcome of the tests. For example, the Beijing strains of M. tuberculosis are prevalent in East Asia, and in particular, the K strain and related strains of the Beijing family, are most frequently isolated during school outbreaks of TB in South Korea. From comparison of genome sequences between M. tuberculosis K strain and the H37Rv strain, a non-Beijing type, we identified a K strain-specific gene, InsB, which has substantial homology with the ESAT-6-like proteins. This study was, therefore, initiated to characterize the InsB protein for its immunogenicity in mice and to confirm its expression in TB patients by detecting antibodies to the protein. The InsB gene was cloned from M. tuberculosis K strain and expressed in Escherichia coli. The recombinant InsB protein was used for immunization of mice. All mice showed strong antibody responses to the InsB protein, and splenocytes stimulated with InsB showed strong IFN-γ and IL-17 responses and a weak IL-2 response, all of which have been implicated in disease expression and used for the immunodiagnosis of TB. Serum samples from TB patients also showed significant antibody responses to the InsB protein as compared to healthy control samples. These results indicate that the InsB protein is an M. tuberculosis K-strain-specific antigen that could further improve the current immunodiagnostic
methods
, especially for the South Korean population.

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Detection of Inhibitors of Phenotypically Drug-tolerant Mycobacterium tuberculosis Using an In Vitro Bactericidal Screen: Ian M. Bassett , Shichun Lun , William R. Bishai , Haidan Guo , Joanna R. Kirman , Mudassar Altaf , Ronan F. O’Toole; J. Microbiol. 2013;51(5):651-658. Published online June 25, 2013; DOI: https://doi.org/10.1007/s12275-013-3099-4

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Many whole cell screens of chemical libraries currently in use are based on inhibition of bacterial growth. The goal of this study was to develop a chemical library screening model that enabled detection of compounds that are active against drug-tolerant non-growing cultures of Mycobacterium tuberculosis. An in vitro model of low metabolically active mycobacteria was established with 8 and 30 day old cultures of M. smegmatis and M. tuberculosis, respectively. Reduction of resazurin was used as a measure of viability and the assay was applied in screens of chemical libraries for bactericidal compounds. The model provided cells that were phenotypically-resilient to killing by first and second-line clinical drugs including rifampicin. Screening against chemical libraries identified proteasome inhibitors, NSC310551 and NSC321206, and a structurally-related series of thiosemicarbazones, as having potent killing activity towards aged cultures. The inhibitors were confirmed as active against virulent M. tuberculosis strains including multi- and extensively-drug resistant clinical isolates. Our library screen enabled detection of compounds with a potent level of bactericidal activity towards phenotypically drug-tolerant cultures of M. tuberculosis.

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Cytotoxicity and activity of thiosemicarbazones and semicarbazones in Mycobacterium tuberculosis: a systematic review
Ana Paula Desiree de Oliveira, Eloísa Gibin Sampiron, Jean Eduardo Meneguello, Andressa Lorena Ieque, Katiany Rizzieri Caleffi Ferracioli, Rosilene Fressatti Cardoso, Fábio Vandresen, Regiane Bertin de Lima Scodro
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ChemistryOpen.2015; 4(3): 342. CrossRef

Protein-Protein Interactions between Histidine Kinases and Response Regulators of Mycobacterium tuberculosis H37Rv: Ha-Na Lee , Kwang-Eun Jung , In-Jeong Ko , Hyung Suk Baik , Jeong-Il Oh; J. Microbiol. 2012;50(2):270-277. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-2050-4

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Using yeast two-hybrid assay, we investigated protein-protein interactions between all orthologous histidine kinase (HK)/response regulator (RR) pairs of M. tuberculosis H37Rv and identified potential protein-protein interactions between a noncognate HK/RR pair, DosT/NarL. The protein interaction between DosT and NarL was verified by phosphotransfer reaction from DosT to NarL. Furthermore, we found that the DosT and DosS HKs, which share considerable sequence similarities to each other and form a twocomponent system with the DosR RR, have different crossinteraction capabilities with NarL: DosT interacted with NarL, while DosS did not. The dimerization domains of DosT and DosS were shown to be sufficient to confer specificity for DosR, and the different cross-interaction abilities of DosS and DosT with NarL were demonstrated to be attributable to variations in the amino acid sequences of the α2-helices of their dimerization domains.

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PLOS Genetics.2023; 19(12): e1011070. CrossRef
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Molecules and Cells.2017; 40(9): 632. CrossRef
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FEMS Microbiology Letters.2013; 343(1): 26. CrossRef

Research Support, N.I.H., Extramural

Sulfolipid Accumulation in Mycobacterium tuberculosis Disrupted in the mce2 Operon: Olivera Marjanovic , Anthony T. Iavarone , Lee W. Riley; J. Microbiol. 2011;49(3):441-447. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0435-4

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Mycobacterium tuberculosis, the causative agent of tuberculosis, has a lipid-rich cell wall that serves as an effective barrier against drugs and toxic host cell products, which may contribute to the organism’s persistence in a host. M. tuberculosis contains four homologous operons called mce (mce1-4) that encode putative ABC transporters involved in lipid importation across the cell wall. Here, we analyzed the lipid composition of M. tuberculosis disrupted in the mce2 operon. High resolution mass spectrometric and thin layer chromatographic analyses of the mutant’s cell wall lipid extracts showed accumulation of SL-1 and SL1278 molecules. Radiographic quantitative analysis and densitometry revealed 2.9, 3.9 and 9.8-fold greater amount of [35S] SL-1 in the mce2 operon mutant compared to the wild type M. tuberculosis during the early/mid logarithmic, late logarithmic and stationary phase of growth in liquid broth, respectively. The amount of [35S] SL1278 in the mutant also increased progressively over the same growth phases. The expression of the mce2 operon genes in the wild type strain progressively increased from the logarithmic to the stationary phase of bacterial growth in vitro, which inversely correlated with the proportion of radiolabel incorporation into SL-1 and SL1278 at these phases. Since the mce2 operon is regulated in wild type M. tuberculosis, its cell wall may undergo changes in SL-1 and SL1278 contents during a natural course of infection and this may serve as an important adaptive strategy for M. tuberculosis to maintain persistence in a host.

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Future Microbiology.2023; 18(18): 1381. CrossRef
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Genome Biology and Evolution.2019; 11(8): 2420. CrossRef
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Scientific Reports.2017;[Epub] CrossRef
Molecular and functional analysis of the mce4 operon in Mycobacterium smegmatis
Julia García‐Fernández, Kadamba Papavinasasundaram, Beatriz Galán, Christopher M. Sassetti, José L. García
Environmental Microbiology.2017; 19(9): 3689. CrossRef
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Julia García‐Fernández, Kadamba Papavinasasundaram, Beatriz Galán, Christopher M. Sassetti, José L. García
Environmental Microbiology.2017; 19(7): 2564. CrossRef
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Peter J. Crick, Xue Li Guan
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2016; 1861(1): 60. CrossRef
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Biochemical Pharmacology.2015; 96(3): 159. CrossRef
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Article

Genotypic and Phenotypic Characteristics of Tunisian Isoniazid-Resistant Mycobacterium tuberculosis Strains: Alya Soudani , Meriem Zribi , Feriel Messaadi , Taieb Messaoud , Afef Masmoudi , Mohamed Zribi , Chedlia Fendri; J. Microbiol. 2011;49(3):413-417. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0268-1

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Forty three isoniazid (INH)-resistant Mycobacterium tuberculosis isolates were characterized on the basis of the most common INH associated mutations, katG315 and mabA -15C→T, and phenotypic properties (i.e. MIC of INH, resistance associated pattern, and catalase activity). Typing for resistance mutations was performed by Multiplex Allele-Specific PCR and sequencing reaction. Mutations at either codon were detected in 67.5% of isolates: katG315 in 37.2, mabA -15C→T in 27.9 and both of them in 2.4%, respectively. katG sequencing showed a G insertion at codon 325 detected in 2 strains and leading to amino acid change T326D which has not been previously reported. Distribution of each mutation, among the investigated strains, showed that katG S315T was associated with multiple-drug profile, high-level INH resistance and loss or decreased catalase activity; whereas the mabA -15C→T was more prevalent in mono-INH resistant isolates, but it was not only associated with a low-level INH resistance. It seems that determination of catalase activity aids in the detection of isolates for which MICs are high and could, in conjunction with molecular methods, provide rapid detection of most clinical INH-resistant strains.

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Research Support, Non-U.S. Gov'ts

Altered Protein Expression Patterns of Mycobacterium tuberculosis Induced by ATB107: Hongbo Shen , Enzhuo Yang , Feifei Wang , Ruiliang Jin , Shengfeng Xu , Qiang Huang , Honghai Wang; J. Microbiol. 2010;48(3):337-346. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-010-9315-6

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ATB107 is a potent inhibitor of indole-3-glycerol phosphate synthase (IGPS). It can effectively inhibit the growth of clinical isolates of drug-resistant Mycobacterium tuberculosis strains as well as M. tuberculosis H37Rv. To investigate the mechanism of ATB107 action in M. tuberculosis, two-dimensional gel electrophoresis coupled with MALDI-TOF-MS analysis (2-DE-MS) was performed to illustrate alterations in the protein expression profile in response to ATB107. Results show that ATB107 affected tryptophan biosynthesis by decreasing the expression of protein encoded by Rv3246c, the transcriptional regulatory protein of MtrA belonging to the MtrA-MtrB two-component regulatory system, in both drug-sensitive and drug-resistant virulent strains. ATB107 might present a stress condition similar to isoniazid (INH) or ethionamide for M. tuberculosis since the altered expression in response to ATB107 of some genes, such as Rv3140, Rv2243, and Rv2428, is consistent with INH or ethionamide treatment. After incubation with ATB107, the expression of 2 proteins encoded by Rv0685 and Rv2624c was down-regulated while that of protein encoded by Rv3140 was up-regulated in all M. tuberculosis strains used in this study. This may be the common response to tryptophan absence; however, relations to ATB107 are unknown and further evaluation is warranted.

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Infectious Disorders - Drug Targets .2019; 19(1): 73. CrossRef
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Margot J. Zaccardi, Eric M. Yezdimer, David D. Boehr
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Production of and Applications for a Polyclonal IgY Diagnostic Reagent Specific for Mycobacterium avium subsp. paratuberculosis: Sung Jae Shin , Seung-Sub Lee , Elizabeth J. B. Manning , Michael T. Collins; J. Microbiol. 2009;47(5):600-609. Published online October 24, 2009; DOI: https://doi.org/10.1007/s12275-009-0052-7

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Antibodies specific to the cell surface antigens of Mycobacterium avium subsp. paratuberculosis (MAP) have multiple useful applications, e.g. organism detection, immunoconcentration, and cell visualization. The aim of this study was to produce and compare polyclonal antibodies for such research and diagnostic purposes. Three polyclonal antibodies to MAP were produced using sera from immunized rabbits and chickens plus naturally infected cows. Cross-reactive antibodies in each MAP antibody preparation were removed by absorption with heterologous mycobacterial and non-mycobacterial cells. The specificity of each resulting polyclonal antibody preparation was evaluated by ELISA to multiple bacterial cell wall extract antigens. After absorption, chicken anti-MAP IgY had the highest specificity of the three antibody preparations. FITC-labeled anti-MAP IgY was used to effectively locate MAP in macrophages 12 h post-infection. Also, immuno- magnetic beads coated with anti-MAP IgY enhanced recovery of MAP from bacterial suspensions in comparison with non-antibody coated beads. Anti-MAP IgY provides a novel new reagent with broad diagnostic and research applications requiring specific concentration, detection, and quantification of MAP.

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Detection of Mycobacterium avium Subspecies paratuberculosis (MAP) Microorganisms Using Antigenic MAP Cell Envelope Proteins
Shanmugasundaram Karuppusamy, Lucy Mutharia, David Kelton, Brandon Plattner, Sanjay Mallikarjunappa, Niel Karrow, Gordon Kirby
Frontiers in Veterinary Science.2021;[Epub] CrossRef
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Juliana Gómez-Valderrama, Lorena Herrera, Daniel Uribe-Vélez, Miguel López-Ferber, Laura Villamizar
International Journal of Pest Management.2014; 60(2): 136. CrossRef
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Evaluation of Three Molecular Methods of Repetitive Element Loci for Differentiation of Mycobacterium avium subsp. paratuberculosis (MAP): Amr El-Sayed , Abdulwahed Ahmed Hassan , Saleh Natour , Amir Abdulmawjood , Michael Bulte , Wilfried Wolter , Michael Zschock; J. Microbiol. 2009;47(3):253-259. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-008-0257-1

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The aim of the present study is to evaluate the efficiency of three methods to determine the molecular diversity of 34 Mycobacterium avium subsp. paratuberculosis (MAP) strains isolated from 17 cattle herds. The applied methods included the analysis of sequence polymorphism of the mononucleotide (G1 and G2) and trinucleotide sequences (GGT) of the Short Sequence Repeats (SSR) and the determination of size polymorphism of 9 different Mycobacterial Interspersed Repetitive Units (MIRU) and 6 Variable Number Tandem Repeats (VNTR). Sequence analysis of SSR of 34 isolates showed 4, 6, and 2 alleles of G1, G2, and GGT repeats, respectively. The amplification of the investigated 9 MIRU units revealed only two discriminatory genotyping systems (MIRU2 and MIRU3). Out of 6 VNTR PCR differentiation methods, only one method could be recommended for genotyping purposes. The profile 7g-12g-4ggt-II-b-2 of the combination systems G1-G2-GGT-MIRU2-MIRU3-VNTR1658 dominates among the examined isolates and was
detected in 14.7% of the isolates. The use of certain repetitive loci of SSR, MIRU, and VNTR techniques in this study showed greater potential than others for the characterization of MAP isolates. The recommended loci can be used for the epidemiological tracing of MAP field strains and to determine the relationships
between isolates in different herds.

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More insights about genomic population structure of Mycobacterium avium subspecies paratuberculosis (Map) from multiple hosts in west and central provinces of Iran using a boosted genotyping approach
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Comparative Proteomic Analysis of Virulent Korean Mycobacterium tuberculosis K-strain with Other Mycobacteria Strain Following Infection of U-937 Macrophage: Sung Weon Ryoo , Young Kil Park , Sue-Nie Park , Young Soo Shim , Hyunjeong Liew , Seongman Kang , Gill-Han Bai; J. Microbiol. 2007;45(3):268-271.; DOI: https://doi.org/2532 [pii]

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Abstract PDF: In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

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Detection of Hepatitis B Virus and Mycobacterium tuberculosis in Korean Dental Patients: Sun-A Lee , So Young Yoo , Kee-Sung Kay , Joong-Ki Kook; J. Microbiol. 2004;42(3):239-242.; DOI: https://doi.org/2082 [pii]

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Abstract PDF: This study examined the detection rate of the hepatitis B virus (HBV) and Mycobacterium tuberculosis (Mtb) in serum and saliva samples, respectively, from 120 dental patients who were unaware if they have or had either hepatitis or tuberculosis. The frequencies of HBsAg and anti-HBs were determined using an immunochromatic assay. Mtb positivity was determined by the PCR method. Of the 120 patients, 7 (5.8%) were HBV positive and 30 (25.0%) were Mtb positive. This highlights the fact that dental health care workers (DHCWs) can be exposed to the risk of infection from blood- or saliva-borne pathogens as a consequence of their work. Therefore, it is very important to prevent cross infection between patients and dental personnel. Accordingly, laboratory tests prior to surgical treatment are needed to determine the infectious state of dental patients in order to prevent the transmission of infectious diseases in dental clinics.

Partial characterization of proteases from culture filtrate of mycobacterium tuberculosis: Na, Byoung Kuk , Song, Chul Yong , Park, Young Kil , Bai, Gill Han , Ki, Sang Jae; J. Microbiol. 1996;34(2):198-205.

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Abstract PDF: Two proteases were partially characterized from culture filtrate of Mycobacterium, tuberculosis KIT110. Their molecular weights were approximately 200 and 180 kDa, respectively and they exhibited similar enzymatic characteristics. These enzymes were inhibited significantly by EDTA and to some extent by EGTA. Their activity was enhanced by Ca^2+ and Mg^2+ to some degree. However, Cu^2+ and Ag^2+ completely inhibited the enzyme activity at the concentration of 2.5 and 5 mM, respectively. The optimal pH was 7.0 and optimal temperature was around 40℃. These enzymes were rapidly inactivated at 80℃. Therefore, they were heat-labile, neutral metalloproteases. These enzymes exhibited antigenicity shown by their reacting with sera from the partients with pulmonary tuberculosis. These enzymes were able to degrade serum proteins including hemoglobin, bovine serum albumin, lysozyme and immunoglobulin G and structural matrix protein such as type I collagen. Therefore, these enzymes may be thought to contribute to tissue necrosis and pathogenesis during infection.

Eveluation of line probe assay in detecting rifampicin resistance of mycobacterium tuberculosis: Park, Young Kil , Cho, Snag Hyun , Na, Nyoung Kuk , Song, Chul Yong , Bai, gill Han , Kim, Sang Jae; J. Microbiol. 1997;35(3):177-180.

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Abstract PDF: The purpose of this study was to evaluate the efficiency of Line Probe Assay (LiPA) in detecting the rpoB gene mutation of clinically isolated Mycobacterium tuberculosis (MTB) and to compare the level of resistance to the various rifamycins with their mutation sites. The mutation in the rpoB gene was found in 84 (97.6%) out of 86 rifampicin (RMP) resistant strains as determined by LiPA. No mutation was observed in 2 RMP resistant strains and in any of 38 RMP susceptible strains tested. Only one of 3 strains with Δ5/R5, one of 2 strains with Δ3, and one of 3 strains with Δ2/R2 LiPA profile showed a slightly lower level of resistance to the rifapentine than the other strains. Although we could not find correlations between mutation sites in the rpoB gene and the level of susceptibility to the various rifamycins, the LiPA is recommended as a fast screening tool for detection of RMP resistant MTB.

A Simple and Rapid Molecular Typing of Mycobacterium tuberculosis by Polyberase Chain Reaction: Lee, Hye Young , Bangm Hye Eun , Lee, Jin Hee , Myung, Han Jung , Kim, Joo Deuk , Cho, Sang Nae; J. Microbiol. 1998;36(2):124-129.

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Abstract PDF: As an attempt to evaluate a molecular tool fingerprinting clinical isolates of Mycobacterium tuberculosis, a PCR-based typing method, so-called outward-PCR, was employed in this study. Outward-PCR used in this study was designed to amplify the wequences in-between two IS6110 elements. A total of 81 M. tuberculosis isolates including 73 Korean and 8 Philippine isolates were subjected to PCR amplification and the profiles of the agarose gel electrophoresis were analyzed. In brief, under the PCR conditions used in this study, the 81 clinical isolates were classified into 33 distinctive sub-groups. Among these, 5 sub-groups represented major clusters with 7 to 11 clinical isolates belonging to each suv-group. The banding patterns were clear and reproducible, implying that this repid and simple PCR-based typing method can be a valuable tool for typing clinical isolates of M. tuberculosis.

The Value of Submitting Multiple Sputum Specimens for Accurate Diagnosis of Pulmonary Tuberculosis: Ozgul Kisa , Ali Albay , Orhan Baylan , Levent Doganci; J. Microbiol. 2002;40(4):301-304.

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Abstract PDF: Is a multiple number of sputum specimens necessary for the diagnosis of pulmonary tuberculosis? To answer this question, 6844 respiratory specimens obtained from previously untreated patients suspected of having pulmonary tuberculosis between 1998 and 2001 were evaluated retrospectively. All of the specimens were evaluated by acid fast bacilli smear and BACTEC 460 TB culture system. A total of 785 (11%) specimens from 353 patients were positive for Mycobacterium tuberculosis complex. For 76% (270/353) of these patients the organism was detected from sputum specimens collected sequentially for daily basis. Mycobacterium tuberculosis was isolated in the first, second and third samples of the majority (98%, 195/199) of patients who had three or more sputum samples sent to the laboratory. Our results indicate that, we could carry out Mycobacterium tuberculosis isolation in the first, second and third sputum samples of the overwhelming majority of the patients and the diagnostic value of four or more sputum specimens submitted to the laboratory was very low (2%). We recommend that, for definitive and cost-effective diagnosis of pulmonary tuberculosis at least three sequential sputum specimens be collected for all patients suspected pulmonary tuberculosis.

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