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- The Identification of a Novel Pleurotus ostreatus dsRNA Virus and Determination of the Distribution of Viruses in Mushroom Spores
- Yeo Jin Kim , Ji Yeon Kim , Ji Hye Kim , Seon Mee Yoon , Young-Bok Yoo , Se Won Yie
- J. Microbiol. 2008;46(1):95-99.
- DOI: https://doi.org/10.1007/s12275-007-0171-y
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- 11 Crossref
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Abstract
PDF - Double-stranded RNAs and virus particles were identified in Pleurotus ostreatus strain Shin-Nong in Korea. Isometric virus particles with a diameter of 33 nm were purified, which are similar to other Pleurotus viruses reported previously. This strain contains 5 dsRNAs, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. The virus particles contain 2 dsRNAs, designated RNA-1 (2.5 kb), and RNA-2 (2.4 kb) which is a typical pattern of Partitiviridae. A non-encapsidated dsRNA of about 8.0 kb also was identified. Partial cDNA from RNA-1 was cloned, and sequence analysis revealed that this gene codes for RdRp. The comparison of the sequence from partial cDNA clone showed 35% amino acid homology with the C-terminal end of the RdRp gene of Helicobasidum mompa virus and Rosalinia necatrix virus. Specific primers designed from the partial sequences successfully amplified RT-PCR product from the infected mycelium and a single spore culture. We used these primers to determine the pattern of distribution of viruses in spores. Of the 96 different single spore cultures generated from Shin-Nong strain, a specific RT-PCR product was identified in 25 cultures, indicating that about 26% of basidiospores contain viruses.
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- Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection in Pleurotus ostreatus
Yifan Wang, Junjie Yan, Guoyue Song, Zhizhong Song, Matthew Shi, Haijing Hu, Lunhe You, Lu Zhang, Jianrui Wang, Yu Liu, Xianhao Cheng, Xiaoyan Zhang
International Journal of Molecular Sciences.2024; 25(17): 9749. CrossRef - Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius
Xiaoyan Zhang, Haijing Hu, Yanxiang Zhao, Yifan Wang, Wenjing Zhang, Lunhe You, Jianrui Wang, Yu Liu, Xianhao Cheng
International Journal of Molecular Sciences.2023; 24(13): 10584. CrossRef - Complete genome sequence of a novel mycovirus from Pleurotus citrinopileatus
Xuefei Li, Shiyu Li, Weiqi Yin, Frederick Leo Sossah, Bing Song, Yu Li, Bo Zhang
Archives of Virology.2023;[Epub] CrossRef - Preliminary Studies on the Effects of Oyster Mushroom Spherical Virus China Strain on the Mycelial Growth and Fruiting Body Yield of the Edible Mushroom Pleurotus ostreatus
Hai-Jing Hu, Jian-Rui Wang, Xian-Hao Cheng, Yu Liu, Xiao-Yan Zhang
Biology.2022; 11(4): 574. CrossRef - Interruption of an MSH4 homolog blocks meiosis in metaphase I and eliminates spore formation in Pleurotus ostreatus
Brian Lavrijssen, Johan P. Baars, Luis G. Lugones, Karin Scholtmeijer, Narges Sedaghat Telgerd, Anton S. M. Sonnenberg, Arend F. van Peer, Arthur J. Lustig
PLOS ONE.2020; 15(11): e0241749. CrossRef - Viruses infecting macrofungi
Ergin Sahin, Ilgaz Akata
VirusDisease.2018; 29(1): 1. CrossRef - Viral Effects of a dsRNA Mycovirus (PoV-ASI2792) on the Vegetative Growth of the Edible Mushroom Pleurotus ostreatus
Ha-Yeon Song, Hyo-Jin Choi, Hansaem Jeong, Dahye Choi, Dae-Hyuk Kim, Jung-Mi Kim
Mycobiology.2016; 44(4): 283. CrossRef - Brown mushroom symptom expression following infection of an Agaricus bisporus crop with MVX associated dsRNAs
Caoimhe Fleming-Archibald, Angela Ruggiero, Helen M. Grogan
Fungal Biology.2015; 119(12): 1237. CrossRef - Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001
Jie Zhong, Dan Chen, Xiang Hua Lei, Hong Jian Zhu, Jun Zi Zhu, Bi Da Gao
Virus Research.2014; 190: 104. CrossRef - Recombinant expression of the coat protein of Botrytis virus X and development of an immunofluorescence detection method to study its intracellular distribution in Botrytis cinerea
Barbara Boine, Richard L. Kingston, Michael N. Pearson
Journal of General Virology .2012; 93(11): 2502. CrossRef - Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China
Liyou Qiu, Yanpeng Li, Yingmiao Liu, Yuqian Gao, Yuancheng Qi, Jinwen Shen
Fungal Biology.2010; 114(5-6): 507. CrossRef
- Transcriptome Analysis Reveals the Effect of Oyster Mushroom Spherical Virus Infection in Pleurotus ostreatus
- Characteristics of Ustilago maydis Virus of SH14 Killer Strain Isolated in Korea
- Hwang, Seon Hee , Jung, Cheong Hwan , Yue, Se Won
- J. Microbiol. 1995;33(2):154-159.
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Abstract
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- SH-14, a novel killer strain of Ustilago maydis was isolated in Korea. It has been reported in other papers that the toxin specificity and double-stranded RNA pattern of SH-14 strain were different from other laboratory strains. In this paper, we analyzed the biochemical characteristics of U. maydis SH-14 virus. Three distinctive peaks were isolated from CsCl density gradient, designated as top (T), intermediate (I) and bottom (B) components. We found that the densities of each components, 1.285, 1.408 g/㎤, respectively, are very similar to those of other strains. As previously reported by the analysis of dsRNA in each component, the dsRNA segments are separately encapsidated. Capsid protein of SH-14 virus consists of two proteins about 70 Kd shown by SDS-PAGE analysis. Electron microscopic examination of the virus particles revealed that UmV particles are very similar in size and morphology to all isolates as well as all lab-strains. In order to test immunological cross reactivity of UmV, western blot analysis was carried out with antiserum against A8 virus. All capsid protein had positive reaction against A8 antibody which indicated that UmV are immunologically cross-reactive with all isolates from Korea. The results presented in this paper may show that UmV isolated from SH-14 strain has very similar biochemical characteristics to those of other UmV. However, the difference in the toxin specificity and the molecular weight of toxin protein from the SH-14 strain has us to conclude that U. maydis SH-14 strain is a new killer type.
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