Journal of Microbiology

Article

The Regulation of Phosphorus Release by Penicillium chrysogenum in Different Phosphate via the TCA Cycle and Mycelial Morphology: Liyan Wang , Da Tian , Xiaoru Zhang , Mingxue Han , Xiaohui Cheng , Xinxin Ye , Chaochun Zhang , Hongjian Gao , Zhen Li; J. Microbiol. 2023;61(8):765-775. Published online September 4, 2023; DOI: https://doi.org/10.1007/s12275-023-00072-2

503 View
3 Download
7 Web of Science
7 Crossref

Phosphate-solubilizing fungi (PSF) efficiently dissolve insoluble phosphates through the production of organic acids. This study investigates the mechanisms of organic acid secretion by PSF, specifically Penicillium chrysogenum, under tricalcium phosphate ( Ca3(PO4)2, Ca–P) and ferric phosphate ( FePO4, Fe–P) conditions. Penicillium chrysogenum exhibited higher phosphorus (P) release efficiency from Ca-P (693.6 mg/L) than from Fe–P (162.6 mg/L). However, Fe–P significantly enhanced oxalic acid (1193.7 mg/L) and citric acid (227.7 mg/L) production by Penicillium chrysogenum compared with Ca–P (905.7 and 3.5 mg/L, respectively). The presence of Fe–P upregulated the expression of genes and activity of enzymes related to the tricarboxylic acid cycle, including pyruvate dehydrogenase and citrate synthase. Additionally, Fe–P upregulated the expression of chitinase and endoglucanase genes, inducing a transformation of Penicillium chrysogenum mycelial morphology from pellet to filamentous. The filamentous morphology exhibited higher efficiency in oxalic acid secretion and P release from Fe–P and Ca–P. Compared with pellet morphology, filamentous morphology enhanced P release capacity by > 40% and > 18% in Ca–P and Fe–P, respectively. This study explored the strategies employed by PSF to improve the dissolution of different insoluble phosphates.

Citations

Citations to this article as recorded by

Mechanistic insights into the transcriptomic and metabolomic responses of Curcuma wenyujin under high phosphorus stress
Yu Liu, Chen Wang, Wenqing Xu, Ruike Fan, Zhigang Wu, Lishang Dai
BMC Plant Biology.2025;[Epub] CrossRef
Improving Organic Acid Secretion of Aspergillus niger by Overexpression C4-Dicarboxylic Acid Transporters
Yiyang Tan, Shutong Liu, Sheng Wu, Xiaolu Wang, Depei Wang, Xianli Xue
Fermentation.2025; 11(3): 156. CrossRef
Penicillium restrictum Reshapes Root Exudates and Inhibits Peucedanum praeruptorum Bolting Through Field Inoculation and Co‐Cultivation
Cheng Song, Haoyu Pan, Yuanyuan Wang, Ranran Liao, Muhammad Arif, Haiyu Wang, Shanyong Yi, Bangxing Han
Plant Pathology.2025; 74(7): 2035. CrossRef
Boosting plant welfare and rhizospheric health through the application of phosphorus and potassium-solubilizing fungi from compost and vermicompost
A.J. Toribio, F. Suárez-Estrella, M.M. Jurado, J.A. López-González, M.R. Martínez-Gallardo, M.J. Estrella-González, M.J. López
Biocatalysis and Agricultural Biotechnology.2025; 68: 103741. CrossRef
Integrated Proteomic and Physiological Profiling of Phosphate Stress Response in Potato (Solanum tuberosum L.)
Lulu Xia, Lixiang Cheng, Qingquan Zhang, Feng Zhang
Physiologia Plantarum.2025;[Epub] CrossRef
Pb immobilization by phosphate-solubilizing fungi and fluorapatite under different Mn2+ concentrations
Lei Zhang, Qiang Guan, Yifan Yan, Jiahui Sun, Linyue Xu, Shuo Zhang, Da Tian, Yue He
Frontiers in Microbiology.2025;[Epub] CrossRef
Multi-omics profiling of Curcuma Wenyujin under salt-alkali stress reveals functional genes and associated metabolites
Xinxin Zhang, Ruike Fan, Lieyang Pan, Xiajuan Jiang, Zihan Sun, Zhigang Wu, Xi Bao, Lishang Dai
BMC Plant Biology.2025;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Replication and Pathogenesis of the Pandemic (H1N1) 2009 Influenza Virus in Mammalian Models: Donghyok Kwon , Kyeongcheol Shin , Seungtae Kim , Yooncheol Ha , Jang-Hoon Choi , Jeong Seon Yang , Joo-Yeon Lee , Chanhee Chae , Hee-Bok Oh , Chun Kang; J. Microbiol. 2010;48(5):657-662. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0120-z

298 View
0 Download
18 Crossref

Abstract PDF

This study aimed to characterize the replication and pathogenic properties of a Korean pandemic (H1N1) 2009 influenza virus isolate in ferrets and mice. Ferrets infected with A/Korea/01/2009 (H1N1) virus showed mild clinical signs. The virus replicated well in lungs and slightly in brains with no replication in any other organs. Severe bronchopneumonia and thickening of alveolar walls were detected in the lungs. Viral antigens were detected in the bronchiolar epithelial cells, in peribronchial glands with severe peribronchitis and in cells present in the alveoli. A/Korea/01/2009 (H1N1) virus-infected mice showed weight loss and pathological lung lesions including perivascular cuffing, interstitial pneumonia and alveolitis. The virus replicated highly in the lungs and slightly in the nasal tissues. Viral antigens were detected in bronchiolar epithelial cells, pneumocytes and interstitial macrophages. However, seasonal H1N1 influenza virus did not replicate in the lungs of ferrets, and viral antigens were not detected. Thus, this Korean pandemic (H1N1) 2009 isolate infected the lungs of ferrets and mice successfully and caused more pathological lesions than did the seasonal influenza virus.

Citations

Citations to this article as recorded by

Comparative pathogenicity of influenza virus-induced pneumonia mouse model following intranasal and aerosolized intratracheal inoculation
Xiu-Yu Jin, Hui-Ying Yang, Guang-Yu Zhao, Chen-Xi Dai, Zai-Qing Zhang, Dong-Sheng Zhou, Qi Yin, Er-Hei Dai
Virology Journal.2024;[Epub] CrossRef
Pathogenesis and transmission of human seasonal and swine-origin A(H1) influenza viruses in the ferret model
Joanna A. Pulit-Penaloza, Nicole Brock, Joyce Jones, Jessica A. Belser, Yunho Jang, Xiangjie Sun, Sharmi Thor, Claudia Pappas, Natosha Zanders, Terrence M. Tumpey, C. Todd Davis, Taronna R. Maines
Emerging Microbes & Infections.2022; 11(1): 1452. CrossRef
Histological Evidence of Pulmonary Microthrombosis and Vasculitis in Life-Threatening Respiratory Virus Diseases
Heather W Dolby, Philippe Potey, Annika B Wilder-Smith, Sara Clohisey, Jonathan E Millar, J Kenneth Baillie, David A Dorward, Christopher D Lucas, Clark D Russell
Open Forum Infectious Diseases.2021;[Epub] CrossRef
In Vivo Models to Study the Pathogenesis of Extra-Respiratory Complications of Influenza A Virus Infection
Edwin Veldhuis Kroeze, Lisa Bauer, Valentina Caliendo, Debby van Riel
Viruses.2021; 13(5): 848. CrossRef
Evaluation of Protective Immunity of Peptide Vaccines Composed of a 15-mer N-terminal Matrix Protein 2 and a Helper T-Cell Epitope Derived from Influenza A Virus
Jeong-Ki Kim, Sinyoung Cheong, Myung Kyu Lee
Immune Network.2019;[Epub] CrossRef
Sowing the Seeds of a Pandemic? Mammalian Pathogenicity and Transmissibility of H1 Variant Influenza Viruses from the Swine Reservoir
Joanna A. Pulit-Penaloza, Jessica A. Belser, Terrence M. Tumpey, Taronna R. Maines
Tropical Medicine and Infectious Disease.2019; 4(1): 41. CrossRef
Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation
Paloma Rodriguez, M Isabel Pérez-Morgado, Víctor M Gonzalez, M Elena Martín, Amelia Nieto
Molecular Therapy - Nucleic Acids.2016; 5: e308. CrossRef
Prevalence of gastrointestinal symptoms in patients with influenza, clinical significance, and pathophysiology of human influenza viruses in faecal samples: what do we know?
Laetitia Minodier, Remi N. Charrel, Pierre-Emmanuel Ceccaldi, Sylvie van der Werf, Thierry Blanchon, Thomas Hanslik, Alessandra Falchi
Virology Journal.2015;[Epub] CrossRef
Genome Wide Identification of SARS-CoV Susceptibility Loci Using the Collaborative Cross
Lisa E. Gralinski, Martin T. Ferris, David L. Aylor, Alan C. Whitmore, Richard Green, Matthew B. Frieman, Damon Deming, Vineet D. Menachery, Darla R. Miller, Ryan J. Buus, Timothy A. Bell, Gary A. Churchill, David W. Threadgill, Michael G. Katze, Leonard
PLOS Genetics.2015; 11(10): e1005504. CrossRef
Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea
Donghyok Kwon, Kyeongcheol Shin, Su-Jin Kim, Joo-Yeon Lee, Chun Kang
Virus Research.2014; 185: 41. CrossRef
Animal models for influenza virus pathogenesis, transmission, and immunology
Rajagowthamee R. Thangavel, Nicole M. Bouvier
Journal of Immunological Methods.2014; 410: 60. CrossRef
Experimental infection and natural contact exposure of ferrets with canine influenza virus (H3N2)
Yu-Na Lee, Dong-Hun Lee, Jae-Keun Park, Seong-Su Yuk, Jung-Hoon Kwon, Sang-Soep Nahm, Joong-Bok Lee, Seung-Yong Park, In-Soo Choi, Chang-Seon Song
Journal of General Virology .2013; 94(2): 293. CrossRef
DBA/2 mouse as an animal model for anti-influenza drug efficacy evaluation
Jin Il Kim, Sehee Park, Sangmoo Lee, Ilseob Lee, Jun Heo, Min-Woong Hwang, Joon-Yong Bae, Donghwan Kim, Seok-Il Jang, Mee Sook Park, Man-Seong Park
Journal of Microbiology.2013; 51(6): 866. CrossRef
Characterization In Vitro and In Vivo of a Pandemic H1N1 Influenza Virus from a Fatal Case
Ariel Rodriguez, Ana Falcon, Maria Teresa Cuevas, Francisco Pozo, Susana Guerra, Blanca García-Barreno, Pamela Martinez-Orellana, Pilar Pérez-Breña, Maria Montoya, Jose Antonio Melero, Manuel Pizarro, Juan Ortin, Inmaculada Casas, Amelia Nieto, Andreas Zi
PLoS ONE.2013; 8(1): e53515. CrossRef
Comparison of Children Hospitalized With Seasonal Versus Pandemic Influenza A, 2004–2009
Dat Tran, Wendy Vaudry, Dorothy L. Moore, Julie A. Bettinger, Scott A. Halperin, David W. Scheifele, Samina Aziz
Pediatrics.2012; 130(3): 397. CrossRef
Susceptibility of human H3N2 influenza virus to oseltamivir in South Korea, 2009–2011
Sehee Park, Jin Il Kim, Ilseob Lee, Sangmoo Lee, Min-Woong Hwang, Joon-Yong Bae, Jun Heo, Eun-Joo Lim, Won-Seok Seok, Hee Jin Cheong, Joon Young Song, Woo Joo Kim, Man-Seong Park
Journal of Microbiology.2012; 50(6): 1067. CrossRef
Influenza A(H1N1)pdm09 Virus Suppresses RIG-I Initiated Innate Antiviral Responses in the Human Lung
Wenxin Wu, Wei Zhang, J. Leland Booth, Jordan P. Metcalf, Michael CW. Chan
PLoS ONE.2012; 7(11): e49856. CrossRef
Pandemic H1N1 influenza virus causes a stronger inflammatory response than seasonal H1N1 influenza virus in ferrets
Young Myong Kang, Byung Min Song, Joo Sub Lee, Hyun Soo Kim, Sang Heui Seo
Archives of Virology.2011; 156(5): 759. CrossRef

Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models: Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi; J. Microbiol. 2012;50(3):487-488.

300 View
1 Download

Abstract PDF: The threat of a highly pathogenic avian influenza (HPAI) H5N1 virus causing the next pandemic remains a major concern. In this study, we evaluated the immunogenicity and efficacy of an inactivated whole-virus H5N1 pre-pandemic vaccine (MG1109) formulated by Green Cross Co., Ltd containing the hemagglutinin (HA) and neuraminidase (NA) genes of the clade 1 A/Vietnam/1194/04 virus in the backbone of A/Puerto Rico/8/34 (RgVietNam/04xPR8/34). Administration of the MG1109 vaccine (2-doses) in mice and ferrets elicited high HI and SN titers in a dose-dependent manner against the homologous (RgVietNam/04xPR8/34) and various heterologous H5N1 strains, (RgKor/W149/06xPR8/34, RgCambodia/04xPR8/34, RgGuangxi/05xPR8/34), including a heterosubtypic H5N2 (A/Aquatic bird/orea/W81/05) virus. However, efficient cross-reactivity was not observed against heterosubtypic H9N2 (A/Ck/Korea/H0802/08) and H1N1 (PR/8/34) viruses. Mice immunized with 1.9 μg HA/dose of MG1109 were completely protected from lethal challenge with heterologous wild-type HPAI H5N1 A/EM/Korea/W149/06 (clade 2.2) and mouse-adapted H5N2 viruses. Furthermore, ferrets administered at least 3.8 μg HA/dose efficiently suppressed virus growth in the upper respiratory tract and lungs. Vaccinated mice and ferrets also demonstrated attenuation of clinical disease signs and limited virus spread to other organs. Thus, this vaccine provided immunogenic responses in mouse and ferret models even against challenge with heterologous HPAI H5N1 and H5N2 viruses. Since the specific strain of HPAI H5N1 virus that would potentially cause the next outbreak is unknown, pre-pandemic vaccine preparation that could provide crossprotection against various H5 strains could be a useful approach in the selection of promising candidate vaccines in the future.

First
Prev
Page of 1
Next
Last

Search