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- Inhibition of cardiolipin biosynthesis partially suppresses the sensitivity of an Escherichia coli mutant lacking OmpC to envelope stress
- Dae-Beom Ryu, Umji Choi, Gyubin Han, Chang-Ro Lee
- J. Microbiol. 2025;63(11):e2507004. Published online November 30, 2025
- DOI: https://doi.org/10.71150/jm.2507004
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Abstract
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Supplementary Material -
Porins in the outer membrane (OM) of Gram-negative bacteria play two main functions: passage of various extracellular molecules and maintenance of membrane integrity. OmpC, a non-specific porin, is involved in both functions; however, the exact mechanism of maintenance of membrane integrity remains unknown. In this study, we found that inhibiting cardiolipin biosynthesis partially restored the growth defect of the ompC mutant under envelope stress. Among the three enzymes involved in cardiolipin biosynthesis, ClsABC, this effect is primarily associated with ClsA. Notably, the deletion of ClsA also suppressed the similar phenotypes of an Escherichia coli mutant lacking YhdP, a transmembrane protein involved in phospholipid transport from the inner membrane to the OM. Collectively, these results imply that OmpC may contribute to membrane integrity, partially through mechanisms linked to transport or biosynthesis of phospholipids such as cardiolipin.
- Phenotypic characterization of a conserved inner membrane protein YhcB in Escherichia coli
- Chul Gi Sung , Umji Choi , Chang-Ro Lee
- J. Microbiol. 2020;58(7):598-605. Published online April 22, 2020
- DOI: https://doi.org/10.1007/s12275-020-0078-4
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- 6 Web of Science
- 5 Crossref
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Abstract
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- Although bacteria have diverse membrane proteins, the function of many of them remains unknown or uncertain even in Escherichia coli. In this study, to investigate the function of hypothetical membrane proteins, genome-wide analysis of phenotypes of hypothetical membrane proteins was performed under various envelope stresses. Several genes responsible for adaptation to envelope stresses were identified. Among them, deletion of YhcB, a conserved inner membrane protein of unknown function, caused high sensitivities to various envelope stresses and increased membrane permeability, and caused growth defect under normal growth conditions. Furthermore, yhcB deletion resulted in morphological aberration, such as branched shape, and cell division defects, such as filamentous growth and the generation of chromosome- less cells. The analysis of antibiotic susceptibility showed that the yhcB mutant was highly susceptible to various anti-folate antibiotics. Notably, all phenotypes of the yhcB mutant were completely or significantly restored by YhcB without the transmembrane domain, indicating that the localization of YhcB on the inner membrane is dispensable for its function. Taken together, our results demonstrate that YhcB is involved in cell morphology and cell division in a membrane localization-independent manner.
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Citations
Citations to this article as recorded by
- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria
Elayne M Fivenson, Laurent Dubois, Thomas G Bernhardt
Current Opinion in Microbiology.2024; 79: 102479. CrossRef - Loss of YhcB results in overactive fatty acid biosynthesis
Hannah M. Stanley, M. Stephen Trent, K. Heran Darwin
mBio.2024;[Epub] CrossRef - A New Factor LapD Is Required for the Regulation of LpxC Amounts and Lipopolysaccharide Trafficking
Alicja Wieczorek, Anna Sendobra, Akshey Maniyeri, Magdalena Sugalska, Gracjana Klein, Satish Raina
International Journal of Molecular Sciences.2022; 23(17): 9706. CrossRef - Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth
Emily C. A. Goodall, Georgia L. Isom, Jessica L. Rooke, Karthik Pullela, Christopher Icke, Zihao Yang, Gabriela Boelter, Alun Jones, Isabel Warner, Rochelle Da Costa, Bing Zhang, James Rae, Wee Boon Tan, Matthias Winkle, Antoine Delhaye, Eva Heinz, Jean-F
PLOS Genetics.2021; 17(12): e1009586. CrossRef - The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner
Han Byeol Lee, Si Hyoung Park, Chang-Ro Lee
Journal of Microbiology.2021; 59(7): 666. CrossRef
- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria
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