Gram-negative bacteria secrete outer membrane vesicles
(OMVs) that play critical roles in intraspecies, interspecies,
and bacteria-environment interactions. Some OMVs, such
as those produced by Pseudomonas aeruginosa, have previously
been shown to possess antimicrobial activity against
competitor species. In the current study, we demonstrate that
OMVs from Burkholderia thailandensis inhibit the growth
of drug-sensitive and drug-resistant bacteria and fungi. We
show that a number of antimicrobial compounds, including
peptidoglycan hydrolases, 4-hydroxy-3-methyl-2-(2-nonenyl)-
quinoline (HMNQ) and long-chain rhamnolipid are
present in or tightly associate with B. thailandensis OMVs.
Furthermore, we demonstrate that HMNQ and rhamnolipid
possess antimicrobial and antibiofilm properties against methicillin-
resistant Staphylococcus aureus (MRSA). These findings
indicate that B. thailandensis secretes antimicrobial OMVs
that may impart a survival advantage by eliminating competition.
In addition, bacterial OMVs may represent an untapped
resource of novel therapeutics effective against biofilm-
forming and multidrug-resistant organisms.
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