Journal of Microbiology

Research Support, Non-U.S. Gov'ts

The Use of Pseudomonas fluorescens P13 to Control Sclerotinia Stem Rot (Sclerotinia sclerotiorum) of Oilseed Rape: Hui Li , Huaibo Li , Yan Bai , Jing Wang , Ming Nie , Bo Li , Ming Xiao; J. Microbiol. 2011;49(6):884-889. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1261-4

192 View
0 Download
28 Scopus

Abstract PDF: Sclerotinia stem rot (SSR) caused by the fungus Sclerotinia sclerotiorum has been an increasing threat to oilseed rape (Brassica napus L.) cultivation. Efficient and environment‐friendly treatments are much needed. Here we focus on microbial control. The Pseudomonas fluorescens P13 that was isolated from oilseed rape cultivation soil, proved to be a useful biocontrol strain for application. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that it was P. fluorescens P13 and that it had a broad antagonistic spectrum, significantly lessening the mycelial growth of S. sclerotiorum by 84.4% and suppressing sclerotial formation by 95‐100%. Scanning electron microscopy studies attested that P13 deformed S. sclerotiorum mycelia when they were cultured together. P13 did not produce chitinase but did produce hydrogen cyanide (HCN) which was likely one of the antagonistic mechanisms. The density of P13 remained at a high level (≥106 CFU/ml) during 5 weeks in the rhizosphere soil and roots. P13 reduced SSR severity at least by 59% in field studies and also promoted seedling growth (p<0.05) at the seedling stage. From these data, our work provided evidence that P13 could be a good alternative biological resource for biocontrol of S. sclerotiorum.

Molecular Analysis of the Copper-Responsive CopRSCD of a Pathogenic Pseudomonas fluorescens Strain: Yong-hua Hu , Hua-lei Wang , Min Zhang , Li Sun; J. Microbiol. 2009;47(3):277-286. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-008-0278-9

283 View
0 Download
24 Crossref

CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.

Citations

Citations to this article as recorded by

Unraveling the biofilm armor: How ROS drives biofilm formation in aquatic pathogen Edwardsiella piscicida
Qingjian Fang, Yin Gou, Hanjie Gu, Qingjuan Wu, Jiaojiao He, Chenghua Li, Bin Sun, Yonghua Hu
Aquaculture.2025; 609: 742935. CrossRef
Microbial Contributions to Heavy Metal Phytoremediation in Agricultural Soils: A Review
Zobia Khatoon, Ma. del Carmen Orozco-Mosqueda, Gustavo Santoyo
Microorganisms.2024; 12(10): 1945. CrossRef
A Copper-Responsive Two-Component System Governs Lipoprotein Remodeling in Listeria monocytogenes
Gloria Komazin, Amena A. Rizk, Krista M. Armbruster, Victoria A. Bonnell, Manuel Llinás, Timothy C. Meredith, Mohamed Y. El-Naggar
Journal of Bacteriology.2023;[Epub] CrossRef
TCS regulator CpxR of Edwardsiella piscicida is vital for envelope integrity by regulating the new target gene yccA, stress resistance, and virulence
Qingjian Fang, Qingjuan Wu, Huiqin Huang, Jiarui Xu, Jianqiang Huang, Hanjie Gu, Yonghua Hu
Aquaculture.2023; 574: 739703. CrossRef
Edwardsiella piscicida YefM-YoeB: A Type II Toxin-Antitoxin System That Is Related to Antibiotic Resistance, Biofilm Formation, Serum Survival, and Host Infection
Dongmei Ma, Hanjie Gu, Yanjie Shi, Huiqin Huang, Dongmei Sun, Yonghua Hu
Frontiers in Microbiology.2021;[Epub] CrossRef
Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus
Majid Rasool Kamli, Nada A. Y. Alzahrani, Nahid H. Hajrah, Jamal S. M. Sabir, Adeel Malik
Microorganisms.2021; 9(3): 499. CrossRef
Edwardsiella piscicida HigB: A type II toxin that is essential to oxidative resistance, biofilm formation, serum survival, intracellular propagation, and host infection
Jinhong Xie, Qianyun Zhao, Huiqin Huang, Zaiguang Fang, Yonghua Hu
Aquaculture.2021; 535: 736382. CrossRef
Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site
Satya Prathyusha Bhamidimarri, Tessa R. Young, Muralidharan Shanmugam, Sandra Soderholm, Arnaud Baslé, Dirk Bumann, Bert van den Berg, Ann M. Stock
PLOS Biology.2021; 19(11): e3001446. CrossRef
The Two-Component System CopRS Maintains Subfemtomolar Levels of Free Copper in the Periplasm of Pseudomonas aeruginosa Using a Phosphatase-Based Mechanism
Lorena Novoa-Aponte, Cheng Xu, Fernando C. Soncini, José M. Argüello, Craig D. Ellermeier
mSphere.2020;[Epub] CrossRef
Characterization of Acinetobacter baumannii Copper Resistance Reveals a Role in Virulence
Caitlin L. Williams, Heather M. Neu, Yonas A. Alamneh, Ryan M. Reddinger, Anna C. Jacobs, Shweta Singh, Rania Abu-Taleb, Sarah L. J. Michel, Daniel V. Zurawski, D. Scott Merrell
Frontiers in Microbiology.2020;[Epub] CrossRef
The direct and indirect effects of environmental toxicants on the health of bumblebees and their microbiomes
Jason A. Rothman, Kaleigh A. Russell, Laura Leger, Quinn S. McFrederick, Peter Graystock
Proceedings of the Royal Society B: Biological Sciences.2020; 287(1937): 20200980. CrossRef
Changes in sediment microbial diversity following chronic copper-exposure induce community copper-tolerance without increasing sensitivity to arsenic
Ayanleh Mahamoud Ahmed, Vincent Tardy, Chloé Bonnineau, Patrick Billard, Stéphane Pesce, Emilie Lyautey
Journal of Hazardous Materials.2020; 391: 122197. CrossRef
Thioredoxin H (TrxH) contributes to adversity adaptation and pathogenicity of Edwardsiella piscicida
Bi-ying Wang, Hui-qin Huang, Shuang Li, Ping Tang, Hao-fu Dai, Jian-an Xian, Dong-mei Sun, Yong-hua Hu
Veterinary Research.2019;[Epub] CrossRef
HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida
Yan-Jie Shi, Qing-Jian Fang, Hui-Qin Huang, Chun-Guang Gong, Yong-Hua Hu
Veterinary Research.2019;[Epub] CrossRef
The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence
Saleh F. Alquethamy, Marjan Khorvash, Victoria G. Pederick, Jonathan J. Whittall, James C. Paton, Ian T. Paulsen, Karl A. Hassan, Christopher A. McDevitt, Bart A. Eijkelkamp
International Journal of Molecular Sciences.2019; 20(3): 575. CrossRef
Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants
Surajit Das, Hirak R. Dash, Jaya Chakraborty
Applied Microbiology and Biotechnology.2016; 100(7): 2967. CrossRef
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity
Yuan-Yuan Sun, Heng Chi, Li Sun
Frontiers in Microbiology.2016;[Epub] CrossRef
Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
L Liu, H Chi, L Sun
Diseases of Aquatic Organisms.2015; 115(1): 67. CrossRef
Edwardsiella tarda Hfq: impact on host infection and global protein expression
Yong-hua Hu, Yong-xin Li, Li Sun
Veterinary Research.2014;[Epub] CrossRef
An Intimate Link: Two-Component Signal Transduction Systems and Metal Transport Systems in Bacteria
Kamna Singh, Dilani B Senadheera, Dennis G Cvitkovitch
Future Microbiology.2014; 9(11): 1283. CrossRef
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
Sr Zhang, L Zhang, L Sun
Diseases of Aquatic Organisms.2014; 110(3): 181. CrossRef
The impact of transition metals on bacterial plant disease
Helen Fones, Gail M. Preston
FEMS Microbiology Reviews.2013; 37(4): 495. CrossRef
Overlapping and unique contributions of two conserved polysaccharide loci in governing distinct survival phenotypes in Vibrio vulnificus
Yunzhi Guo, Dean A. Rowe‐Magnus
Environmental Microbiology.2011; 13(11): 2888. CrossRef
Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain
Yun Sun, Chun-sheng Liu, Li Sun
Vaccine.2010; 28(40): 6603. CrossRef

Physical Analysis of nahQ and tnpA Genes from Pseudomonas fluorescens: Ja-Young Seol , Soon-Young Choi , Kyung Hee Min; J. Microbiol. 2001;39(4):338-342.

203 View
0 Download

Abstract PDF: Pseudomonas fluorescens SM11 is a naphthalene-degrading strain whose dissimilatory genes are chromosomally encoded. We have cloned the 2.9 kb Sal I fragment harboring genes for the naphthalene-degradation upper pathway. The nucleotide sequences were determined to be nahQ, tnpA, and partial regions of nahE genes. The nahQ encodes a protein of 188 amino acid residues with a deduced molecular weight of 20.8 kDa. The high homology with other proteins suggests that NahQ may be an active and useful protein which gives a selective advantage to naphthalene degradation. Transposase(TnpA) encodes a polypeptide chain with a molecular mass of 41.8 kDa consisting of 376 amino acid residues. The deduced anino acid sequence of tnpA revealed 96% idenitity with putative transposase of P. stutzeri OX1. It was assumed that transposase plays an important role in the evolution of the catabolic-pathway in the regulation of nah expression.

First
Prev
Page of 1
Next
Last

Search