Journal Articles
- The Gut Microbiota Mediates the Protective Effects of Spironolactone on Myocardial Infarction
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Lu Li, Jian-Yong Sun, Yu-Lin Li, Shi-Wei Zhu, Sheng-Zhong Duan
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J. Microbiol. 2024;62(10):883-895. Published online September 3, 2024
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DOI: https://doi.org/10.1007/s12275-024-00164-7
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Myocardial infarction (MI) is a type of cardiovascular disease that influences millions of human beings worldwide and has a great rate of mortality and morbidity. Spironolactone has been used as a critical drug for the treatment of cardiac failure and it ameliorates cardiac dysfunction post-MI. Despite these findings, whether there is a relationship between the therapeutic effects of spironolactone and the gut microorganism after MI has not been determined. In our research, we used male C57BL/6 J mice to explore whether the gut microbiota mediates the beneficial function of spironolactone after myocardial infarction.
We demonstrated that deletion of the gut microbiota eliminated the beneficial function of spironolactone in MI mice, displaying exacerbated cardiac dysfunction, cardiac infarct size. In addition, the gut microbiota was altered by spironolactone after sham or MI operation in mice. We also used male C57BL/6 J mice to investigate the function of a probiotic in the myocardial infarction. In summary, our findings reveal a precious role of the gut flora in the therapeutic function of spironolactone on MI.
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- Probiotics and Prebiotics in Post-Myocardial Infarction Rehabilitation: Mechanisms, Benefits, and Future Directions
Georgy Leonov, Elena Livantsova, Yurgita Varaeva, Antonina Starodubova
Current Nutrition Reports.2025;[Epub] CrossRef - Research Trends and Hotspots of Gut Microbiota and Its Metabolites in Cardiovascular Diseases: A Bibliometric Analysis
Kaixuan Zhang, Yajun Shi, Lirong Peng, Xiaofei Zhang, Nanbo Zheng, Jiajing Xin, Junbo Zou, Fei Luan
Journal of Multidisciplinary Healthcare.2025; Volume 18: 5125. CrossRef - Insights into the role of gut microbiota modulation in the management of various cardiovascular diseases: A new approach for improving the efficacy of current cardiovascular medications
Lamiaa A. Ahmed, Khaled F. Al-Massri
European Journal of Pharmacology.2025; 1007: 178210. CrossRef - The role of the gut microbiota in the onset and progression of heart failure: insights into epigenetic mechanisms and aging
Giulia Matacchione, Francesco Piacenza, Lorenzo Pimpini, Yuri Rosati, Serena Marcozzi
Clinical Epigenetics.2024;[Epub] CrossRef
- Effects of Light and Dark Conditions on the Transcriptome of Aging Cultures of Candidatus Puniceispirillum marinum IMCC1322
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Ji Hyen Lee, Hyun-Myung Oh
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J. Microbiol. 2024;62(4):297-314. Published online April 25, 2024
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DOI: https://doi.org/10.1007/s12275-024-00125-0
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To elucidate the function of proteorhodopsin in Candidatus Puniceispirillum marinum strain IMCC1322, a cultivated representative of SAR116, we produced RNA-seq data under laboratory conditions. We examined the transcriptomes of six different cultures, including sets of expression changes under constant dark (DD), constant light (LL), and diel-cycled (LD; 14 h light: 10 h dark) conditions at the exponential and stationary/death phases. Prepared mRNA extracted from the six samples was analyzed on the Solexa Genome Analyzer with 36 cycles.
Differentially expressed genes on the IMCC1322 genome were distinguished as four clusters by K-mean clustering and each CDS (n = 2546) was annotated based on the KEGG BRITE hierarchy. Cluster 0 (n = 1573) covered most constitutive genes including proteorhodopsin, retinoids, and glycolysis/TCA cycle. Cluster 1 genes (n = 754) were upregulated in stationary/death phase under constant dark conditions and included genes associated with bacterial defense, membrane transporters, nitrogen metabolism, and senescence signaling. Cluster 2 genes (n = 197) demonstrated upregulation in exponential phase cultures and included genes involved in genes for oxidative phosphorylation, translation factors, and transcription machinery. Cluster 3 (n = 22) contained light-stimulated upregulated genes expressed under stationary/phases. Stringent response genes belonged to cluster 2, but affected genes spanned various cellular processes such as amino acids, nucleotides, translation, transcription, glycolysis, fatty acids, and cell wall components. The coordinated expression of antagonistic stringent genes, including mazG, ppx/gppA, and spoT/relA may provide insight into the controlled cultural response observed between constant light and constant dark conditions in IMCC1322 cultures, regardless of cell numbers and biomass.
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- Culture-supported ecophysiology of the SAR116 clade demonstrates metabolic and spatial niche partitioning
Jordan T Coelho, Lauren Teubner, Michael W Henson, V Celeste Lanclos, Conner Y Kojima, J Cameron Thrash
The ISME Journal.2025;[Epub] CrossRef - Effect of Light Regime on Candidatus Puniceispirillum marinum IMCC1322 in Nutrient-Replete Conditions
Hyun-Myung Oh, Ji Hyen Lee, Ahyoung Choi, Sung-Hyun Yang, Gyung-Hoon Shin, Sung Gyun Kang, Jang-Cheon Cho, Hak Jun Kim, Kae-Kyoung Kwon
Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef
- Syntaxin17 Restores Lysosomal Function and Inhibits Pyroptosis Caused by Acinetobacter baumannii
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Zhiyuan An, Wenyi Ding
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J. Microbiol. 2024;62(4):315-325. Published online March 7, 2024
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DOI: https://doi.org/10.1007/s12275-024-00109-0
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Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function. This study aimed to explore the role of STX17 in the regulation of pyroptosis induced by A. baumannii. Our findings indicate that overexpression of STX17 enhances autophagosome degradation, increases LAMP1 expression, reduces Cathepsin B release, and improves lysosomal function.
Conversely, knockdown of STX17 suppresses autophagosome degradation, reduces LAMP1 expression, augments Cathepsin B release, and accelerates lysosomal dysfunction. In instances of A. baumannii infection, overexpression of STX17 was found to improve lysosomal function and reduce the expression of mature of GSDMD and IL-1β, along with the release of LDH, thus inhibiting pyroptosis caused by A.
baumannii. Conversely, knockdown of STX17 led to increased lysosomal dysfunction and further enhanced the expression of mature of GSDMD and IL-1β, and increased the release of LDH, exacerbating pyroptosis induced by A. baumannii. These findings suggest that STX17 regulates pyroptosis induced by A. baumannii by modulating lysosomal function.
- Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
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Yongchao Guan , Meng Zhang , Yingda Wang , Zhongzhuo Liu , Zelin Zhao , Hong Wang , Dingjie An , Aidong Qian , Yuanhuan Kang , Wuwen Sun , Xiaofeng Shan
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J. Microbiol. 2022;60(12):1153-1161. Published online November 10, 2022
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DOI: https://doi.org/10.1007/s12275-022-2373-8
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Aeromonas veronii is a pathogen which can induce diseases in
humans, animals and aquatic organisms, but its pathogenic
mechanism and virulence factors are still elusive. In this study,
we successfully constructed a mutant strain (ΔascP) by homologous
recombination. The results showed that the deletion
of the ascP gene significantly down-regulated the expression
of associated effector proteins in A. veronii compared
to its wild type. The adhesive and invasive abilities of ΔascP to
EPC cells were 0.82-fold lower in contrast to the wild strain.
The toxicity of ΔascP to cells was decreased by about 2.91-fold
(1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant
strain of crucian carp was reduced by 19.94-fold, and
the virulence was considerably attenuated. In contrast to the
wild strain, the ΔascP content in the liver and spleen was considerably
lower. The titers of serum cytokines (IL-8, TNF-α,
and IL-1β) in crucian carp after the infection of the ΔascP strain
were considerably lower in contrast to the wild strain. Hence,
the ascP gene is essential for the etiopathogenesis of A. veronii
TH0426.
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- Complete genome sequence and genome-wide transposon mutagenesis enable the determination of genes required for sodium hypochlorite tolerance and drug resistance in pathogen Aeromonas veronii GD2019
Yifan Bu, Chengyu Liu, Yabo Liu, Wensong Yu, Tingjin Lv, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
Microbiological Research.2024; 284: 127731. CrossRef - Construction of the flagellin F mutant of Vibrio parahaemolyticus and its toxic effects on silver pomfret (Pampus argenteus) cells
Yang Li, Chao Liu, Yuechen Sun, Ruijun Wang, Choufei Wu, Hanqu Zhao, Liqin Zhang, Dawei Song, Quanxin Gao
International Journal of Biological Macromolecules.2024; 259: 129395. CrossRef - Ferric uptake regulator (fur) affects the pathogenicity of Aeromonas veronii TH0426 by regulating flagellar assembly and biofilm formation
Jin-shuo Gong, Ying-da Wang, Yan-long Jiang, Di Zhang, Ya-nan Cai, Xiao-feng Shan, He Gong, Hao Dong
Aquaculture.2024; 580: 740361. CrossRef
Reviews
- Current status and perspectives on vaccine development against dengue virus infection
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Jisang Park , Ju Kim , Yong-Suk Jang
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J. Microbiol. 2022;60(3):247-254. Published online February 14, 2022
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DOI: https://doi.org/10.1007/s12275-022-1625-y
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Dengue virus (DENV) consists of four serotypes in the family
Flaviviridae and is a causative agent of dengue fever, dengue
hemorrhagic fever, and dengue shock syndrome. DENV is
transmitted by mosquitoes, Aedes aegypti and A. albopictus,
and is mainly observed in areas where vector mosquitoes live.
The number of dengue cases reported by the World Health
Organization increased more than 8-fold over the last two
decades from 505,430 in 2000 to over 2.4 million in 2010 to
5.2 million in 2019. Although vaccine is the most effective
method
against DENV, only one commercialized vaccine exists,
and it cannot be administered to children under 9 years of
age. Currently, many researchers are working to resolve the
various problems hindering the development of effective dengue
vaccines; understanding of the viral antigen configuration
would provide insight into the development of effective
vaccines against DENV infection. In this review, the current
status and perspectives on effective vaccine development for
DENV are examined. In addition, a plausible direction for
effective vaccine development against DENV is suggested.
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- Nanoparticle and virus-like particle vaccine approaches against SARS-CoV-2
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Chulwoo Kim , Jae-Deog Kim , Sang-Uk Seo
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J. Microbiol. 2022;60(3):335-346. Published online January 28, 2022
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DOI: https://doi.org/10.1007/s12275-022-1608-z
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The global spread of coronavirus disease 2019 caused by severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
infection has provoked an urgent need for prophylactic measures.
Several innovative vaccine platforms have been introduced
and billions of vaccine doses have been administered
worldwide. To enable the creation of safer and more effective
vaccines, additional platforms are under development. These
include the use of nanoparticle (NP) and virus-like particle
(VLP) technology. NP vaccines utilize self-assembling scaffold
structures designed to load the entire spike protein or
receptor-binding domain of SARS-CoV-2 in a trimeric configuration.
In contrast, VLP vaccines are genetically modified
recombinant viruses that are considered safe, as they are
generally replication-defective. Furthermore, VLPs have indigenous
immunogenic potential due to their microbial origin.
Importantly, NP and VLP vaccines have shown stronger immunogenicity
with greater protection by mimicking the physicochemical
characteristics of SARS-CoV-2. The study of NPand
VLP-based coronavirus vaccines will help ensure the development
of rapid-response technology against SARS-CoV-2
variants and future coronavirus pandemics.
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Jinjong Myoung
Journal of Microbiology.2022; 60(3): 235. CrossRef - Immunogenicity and protective potential of chimeric virus-like particles containing SARS-CoV-2 spike and H5N1 matrix 1 proteins
Jing Chen, Wang Xu, Letian Li, Lichao Yi, Yuhang Jiang, Pengfei Hao, Zhiqiang Xu, Wancheng Zou, Peiheng Li, Zihan Gao, Mingyao Tian, Ningyi Jin, Linzhu Ren, Chang Li
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Construction, Characterization, and Application of a Nonpathogenic Virus-like Model for SARS-CoV-2 Nucleocapsid Protein by Phage Display
Yuting Wu, Bing Liu, Zhiwei Liu, Pengjie Zhang, Xihui Mu, Zhaoyang Tong
Toxins.2022; 14(10): 683. CrossRef - Contribution of T- and B-cell intrinsic toll-like receptors to the adaptive immune response in viral infectious diseases
Ejuan Zhang, Zhiyong Ma, Mengji Lu
Cellular and Molecular Life Sciences.2022;[Epub] CrossRef
Journal Articles
- Genome information of the cellulolytic soil actinobacterium Isoptericola dokdonensis DS-3 and comparative genomic analysis of the genus Isoptericola
-
Yurim Bae , Sujin Lee , Kitae Kim , Hyun-Kwon Lee , Soon-Kyeong Kwon , Jihyun F. Kim
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J. Microbiol. 2021;59(11):1010-1018. Published online November 1, 2021
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DOI: https://doi.org/10.1007/s12275-021-1452-6
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421
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4
Web of Science
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4
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Abstract
PDF
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The actinobacterial group is regarded as a reservoir of biologically
active natural products and hydrolytic enzymes with
the potential for biomedical and industrial applications. Here,
we present the complete genome sequence of Isoptericola
dokdonensis DS-3 isolated from soil in Dokdo, small islets
in the East Sea of Korea. This actinomycete harbors a large
number of genes encoding carbohydrate-degrading enzymes,
and its activity to degrade carboxymethyl cellulose into glucose
was experimentally evaluated. Since the genus Isoptericola was
proposed after reclassification based on phylogenetic analysis,
strains of Isoptericola have been continuously isolated from
diverse environments and the importance of this genus in the
ecosystem has been suggested by recent culturomic or metagenomic
studies. The phylogenic relationships of the genus
tended to be closer among strains that had been isolated from
similar habitats. By analyzing the properties of published genome
sequences of seven defined species in the genus, a large
number of genes for carbohydrate hydrolysis and utilization,
as well as several biosynthetic gene clusters for secondary
metabolites, were identified. Genomic information of I. dokdonensis
DS-3 together with comparative analysis of the genomes
of Isoptericola provides insights into understanding
this actinobacterial group with a potential for industrial applications.
-
Citations
Citations to this article as recorded by

- Genomic analysis of Isoptericola halotolerans SM2308 reveals its potential involved in fucoidan degradation
Yu-Qi Zhang, Qi Yuan, Ji-Qing Liu, Xiao-Chen Liang, Jing-Ping Wang, Wen-Xin Jiang, Ping-Yi Li
Marine Genomics.2025; 79: 101165. CrossRef - Assessing hydrocarbon degradation capacity of Isoptericola peretonis sp. nov. and related species: a comparative study
Àngela Vidal-Verdú, Adriel Latorre-Pérez, Javier Pascual, Ruth Mañes-Collado, Aitana Nevot-Terraes, Manuel Porcar
Frontiers in Microbiology.2025;[Epub] CrossRef - From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects
Yu Duan, Limei Chen, Longxue Ma, Farrukh Raza Amin, Yida Zhai, Guofu Chen, Demao Li
Biotechnology Advances.2024; 77: 108460. CrossRef - A comprehensive review on strategic study of cellulase producing marine actinobacteria for biofuel applications
Ashwini John J, Melvin S. Samuel, Muthusamy Govarthanan, Ethiraj Selvarajan
Environmental Research.2022; 214: 114018. CrossRef
- Characterization of the effects of terminators and introns on recombinant gene expression in the basidiomycete Ceriporiopsis subvermispora
-
Dong Xuan Nguyen , Emi Nishisaka , Moriyuki Kawauchi , Takehito Nakazawa , Masahiro Sakamoto , Yoichi Honda
-
J. Microbiol. 2020;58(12):1037-1045. Published online September 30, 2020
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DOI: https://doi.org/10.1007/s12275-020-0213-2
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408
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3
Web of Science
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3
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Abstract
PDF
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Terminators and introns are vital regulators of gene expression
in many eukaryotes; however, the functional importance
of these elements for controlling gene expression in Agaricomycetes
remains unclear. In this study, the effects of Ceriporiopsis
subvermispora terminators and introns on the expression
of a recombinant hygromycin B phosphotransferase
gene (hph) were characterized. Using a transient transformation
system, we proved that a highly active terminator (e.g.,
the gpd terminator) is required for the efficient expression of
the hph gene. Mutational analyses of the C. subvermispora
gpd terminator revealed that hph expression was dictated by
an A-rich region, which included a putative positioning element,
and polyadenylation sites. In contrast, our results indicated
that introns are not required for the expression of
hph directed by the Csβ1-tub and Csgpd promoters in C.
subvermispora. This study provides insights into the functions
and cis-element requirements of transcriptional terminators
in Agaricomycetes, which may be relevant for designing
recombinant genes for this important fungal class.
-
Citations
Citations to this article as recorded by

- Development of a 2A peptide-based multigene expression system and its application for enhanced production of ganoderic acids in Ganoderma lucidum
Qiong Wang, Hong-Jun Liu, Yan Xu, Zi-Xu Wang, Bin Sun, Jun-Wei Xu
Journal of Biotechnology.2024; 393: 109. CrossRef - CRISPR/Cas9 using a transient transformation system in Ceriporiopsis subvermispora
Takehito Nakazawa, Chikako Inoue, Dong Xuan Nguyen, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda
Applied Microbiology and Biotechnology.2022; 106(17): 5575. CrossRef - A promoter assay system using gene targeting in agaricomycetes Pleurotus ostreatus and Coprinopsis cinerea
Dong Xuan Nguyen, Takehito Nakazawa, Genki Myo, Chikako Inoue, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda
Journal of Microbiological Methods.2020; 179: 106053. CrossRef
- Lactobacillus crispatus and its enolase and glutamine synthetase influence interactions between Neisseria gonorrhoeae and human epithelial cells
-
Jagoda Płaczkiewicz , Paulina Chmiel , Ewelina Malinowska , Pawel B , Agnieszka Kwiatek
-
J. Microbiol. 2020;58(5):405-414. Published online April 11, 2020
-
DOI: https://doi.org/10.1007/s12275-020-9505-9
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391
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10
Web of Science
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8
Crossref
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Abstract
PDF
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Neisseria gonorrhoeae, an obligatory human pathogen causes
the sexually transmitted disease gonorrhea, which remains
a global health problem. N. gonorrhoeae primarily infects the
mucosa of the genitourinary tract, which in women, is colonized
by natural microbiota, dominated by Lactobacillus spp.,
that protect human cells against pathogens. In this study, we
demonstrated that precolonization of human epithelial cells
with Lactobacillus crispatus, one of the most prevalent bacteria
in the female urogenital tract, or preincubation with the
L. crispatus enolase or glutamine synthetase impairs the adhesion
and invasiveness of N. gonorrhoeae toward epithelial
cells, two crucial steps in gonococcal pathogenesis. Furthermore,
decreased expression of genes encoding the proinflammatory
cytokines, TNFα and CCL20, which are secreted as
a consequence of N. gonorrhoeae infection, was observed in
N. gonorrhoeae-infected epithelial cells that had been precolonized
with L. crispatus or preincubated with enolase and
glutamine synthetase. Thus, our results indicate that the protection
of human cells against N. gonorrhoeae infection is a
complex process and that L. crispatus and its proteins enolase
and glutamine synthetase can have a potential role in protecting
epithelial cells against gonococcal infection. Therefore,
these results are important since disturbances of the microbiota
or of its proteins can result in dysbiosis, which is associated
with increased susceptibility of epithelium to pathogens.
-
Citations
Citations to this article as recorded by

- Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract
Li Ying Jessie Lau, Siew Young Quek
Food Bioengineering.2024; 3(1): 41. CrossRef - Use of probiotic lactobacilli in the treatment of vaginal infections: In vitro and in vivo investigations
Peng Liu, Yune Lu, Rongguo Li, Xiaodi Chen
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef - Enhanced IgA coating of bacteria in women with Lactobacillus crispatus-dominated vaginal microbiota
Annelot C. Breedveld, Heleen J. Schuster, Robin van Houdt, Rebecca C. Painter, Reina E. Mebius, Charlotte van der Veer, Sylvia M. Bruisten, Paul H. M. Savelkoul, Marjolein van Egmond
Microbiome.2022;[Epub] CrossRef - Molecular Regulatory Mechanisms Drive Emergent Pathogenetic Properties of Neisseria gonorrhoeae
Ashwini Sunkavalli, Ryan McClure, Caroline Genco
Microorganisms.2022; 10(5): 922. CrossRef - Both Neisseria gonorrhoeae and Neisseria sicca Induce Cytokine Secretion by Infected Human Cells, but Only Neisseria gonorrhoeae Upregulates the Expression of Long Non-Coding RNAs
Jagoda Płaczkiewicz, Monika Adamczyk-Popławska, Ewa Kozłowska, Agnieszka Kwiatek
Pathogens.2022; 11(4): 394. CrossRef - Role of the human vaginal microbiota in the regulation of inflammation and sexually transmitted infection acquisition: Contribution of the non-human primate model to a better understanding?
Cindy Adapen, Louis Réot, Elisabeth Menu
Frontiers in Reproductive Health.2022;[Epub] CrossRef - Alterations of Vaginal Microbiota in Women With Infertility and Chlamydia trachomatis Infection
Hongliang Chen, Li Wang, Lanhua Zhao, Lipei Luo, Shuling Min, Yating Wen, Wenbo Lei, Mingyi Shu, Zhongyu Li
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Lactobacillus Cell Surface Proteins Involved in Interaction with Mucus and Extracellular Matrix Components
Lidia Muscariello, Barbara De Siena, Rosangela Marasco
Current Microbiology.2020; 77(12): 3831. CrossRef
- Comparative genomics of Lactobacillus species as bee symbionts and description of Lactobacillus bombintestini sp. nov., isolated from the gut of Bombus ignitus
-
Jun Heo , Soo-Jin Kim , Jeong-Seon Kim , Seung-Beom Hong , Soon-Wo Kwon
-
J. Microbiol. 2020;58(6):445-455. Published online March 28, 2020
-
DOI: https://doi.org/10.1007/s12275-020-9596-3
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409
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14
Web of Science
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15
Crossref
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Abstract
PDF
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The Lactobacillus genus is widely used for fermentation of
plant materials and dairy products. These species are typically
found in highly specialized environments, with the bee gut
serving as one of the niche locations in which Lactobacillus
is detected. Lactobacillus species isolated from the bee gut and
bee-related habitats were phylogenetically classified into three
distinct groups, Lactobacillus kunkeei, Firm-4, and Firm-5.
The L. kunkeei group was clearly differentiated from other
members of the Lactobacillus buchneri group isolated from
non-bee habitats. In comparison with non-bee members of the
L. buchneri group, three bee-symbiotic Lactobacillus groups
had a small-sized genome with low G + C content and showed
a sharp reduction in the number of genes involved in energy
production, carbohydrate transport and metabolism, and
amino acid transport and metabolism. In addition, all three
groups lacked the mutY gene, which encodes A/G-specific
adenine glycosylase. The phylogenetic dendrogram based on
the presence or absence of 1,199 functional genes indicated
that these bee-symbiotic groups experienced convergent evolution.
The occurrence of convergent evolution is thought to
stem from the three bee-symbiotic groups sharing a similar
habitat, i.e., the bee gut. The causative factor underlying genomic
reduction was postulated to be mutY, which was absent
in all three groups. Here, a novel strain, BHWM-4T, isolated
from the gut of Bombus ignites was studied using polyphasic
taxonomy and classified as a new member of the L.
kunkeei group. The strain was Gram-positive, facultative anaerobic,
and rod-shaped. The 16S ribosomal RNA gene sequence
and genome analysis revealed that strain BHWM-4T was
clustered into the L. kunkeei group, forming a compact cluster
with L. kunkeei and Lactobacillus apinorum. Biochemical,
chemotaxonomic, and genotypic data of strain BHWM-4T
supports the proposal of a novel species, Lactobacillus bombintestini
sp. nov., whose type strain is BHWM-4T (= KACC
19317T = NBRC 113067T).
-
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- Tiny but mighty? Overview of a decade of research on nectar bacteria
Sergio Quevedo‐Caraballo, Clara de Vega, Bart Lievens, Tadashi Fukami, Sergio Álvarez‐Pérez
New Phytologist.2025; 245(5): 1897. CrossRef - Captive-rearing changes the gut microbiota of the bumblebee Bombus lantschouensis native to China
Feng Zhou, Shuning Sun, Xinge Song, Yuying Zhang, Zhuanxia Li, Jiani Chen
PeerJ.2025; 13: e18964. CrossRef -
Apilactobacillus intestinapis sp. nov., isolated from the honeybee
Li-Jyun Tsai, Po-Cheng Chen, Wei-Tsu Lan, Yun-Shien Lee, Yu-Chung Chang, Hui-Chung Wu, Chong-You Lin, Yi-Hsien Liu, Jhih-Ti Teng, Yi-Sheng Chen
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef - Comparative genomic analyses reveal carbohydrates-rich environment adaptability of Lentilactobacillus laojiaonis sp. nov. IM3328
Qiuwei Zhao, Huawei Zhu, Xi Tong, Guanhui Bao, Suping Yang, Songtao Wang, Caihong Shen, Yin Li
Food Bioscience.2023; 53: 102737. CrossRef - International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Bifidobacterium, Lactobacillus and related organisms. Minutes of the closed meeting by videoconference, 3 September 2020
Paola Mattarelli, Giovanna E. Felis, B. Pot, Wilhelm H. Holzapfel, Charles M. A. P. Franz
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Levilactobacillus yiduensis sp. nov., Isolated from Zha-chili in Yidu County, Hubei Province, China
Yun Dong, Yurong Wang, Huijun Zhao, Yan Zhang, Haibo Zhang, Yafang Sun, Zhuang Guo
Current Microbiology.2023;[Epub] CrossRef - Influence of social lifestyles on host–microbe symbioses in the bees
Lauren Mee, Seth M. Barribeau
Ecology and Evolution.2023;[Epub] CrossRef - Effect of Probiotic Microorganisms on Catalase Activity, Fractional Composition of Soluble Proteins, and Intestinal Microbiota of Honey Bee
R.S. Fedoruk, I.I. Kovalchuk, A.Z. Pylypets, M.M. Tsap, Y.V. Lesyk, R.L. Androshulik, O.A. Demchenko, N.O. Tymoshok, L.P. Babenko
Mikrobiolohichnyi Zhurnal.2023; 85(4): 46. CrossRef - Philodulcilactobacillus myokoensis gen. nov., sp. nov., a fructophilic, acidophilic, and agar-phobic lactic acid bacterium isolated from fermented vegetable extracts
Tomoaki Kouya, Yohei Ishiyama, Shota Ohashi, Ryota Kumakubo, Takeshi Yamazaki, Toshiki Otaki, Guadalupe Virginia Nevárez-Moorillón
PLOS ONE.2023; 18(6): e0286677. CrossRef - cpn60 barcode sequences accurately identify newly defined genera within the Lactobacillaceae
Ishika Shukla, Janet E. Hill
Canadian Journal of Microbiology.2022; 68(6): 457. CrossRef -
Lactobacillus huangpiensis sp. nov. and Lactobacillus laiwuensis sp. nov., isolated from the gut of honeybee (Apis mellifera)
Ting Ting Li, Chun Tao Gu
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - Genetic variation and microbiota in bumble bees cross-infected by different strains of C. bombi
Seth M. Barribeau, Paul Schmid-Hempel, Jean-Claude Walser, Stefan Zoller, Martina Berchtold, Regula Schmid-Hempel, Niklaus Zemp, Pedro L. Oliveira
PLOS ONE.2022; 17(11): e0277041. CrossRef - Bombilactobacillus apium sp. nov., isolated from the gut of honeybee (Apis cerana)
Jong-Pyo Kang, Yue Huo, Van-An Hoang, Dong-Uk Yang, Deok-Chun Yang, Se-Chan Kang
Archives of Microbiology.2021; 203(5): 2193. CrossRef - List of new names and new combinations that have appeared in effective publications outside of the IJSEM and are submitted for valid publication
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology
.2020; 70(11): 5596. CrossRef - Proposal to reclassify four Lactobacillus species as Apilactobacillus bombintestini, Companilactobacillus suantsaicola, Lactiplantibacillus garii and Levilactobacillus suantsaiihabitans
Paola Mattarelli, Giovanna Felis, Charles M. A. P. Franz, Michael G. Gänzle
International Journal of Systematic and Evolutionary Microbiology
.2019;[Epub] CrossRef
- Lactobacillus plantarum lipoteichoic acid disrupts mature Enterococcus faecalis biofilm
-
A Reum Kim , Minji Kang , Yeon-Jee Yoo , Cheol-Heui Yun , Hiran Perinpanayagam , Kee-Yeon Kum , Seung Hyun Han
-
J. Microbiol. 2020;58(4):314-319. Published online January 28, 2020
-
DOI: https://doi.org/10.1007/s12275-020-9518-4
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458
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21
Web of Science
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22
Crossref
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Abstract
PDF
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Apical periodontitis is caused by biofilm-mediated root canal
infection. Early phase oral bacterial biofilms are inhibited by
Lactobacillus plantarum lipoteichoic acid (Lp.LTA). However,
mature biofilms that develop over 3 weeks are more resistant
to traditional endodontic medicaments. Therefore, this study
examined the effectiveness of Lp.LTA on disrupting mature
Enterococcus faecalis biofilms, and on enhancing the effects
of endodontic medicaments. LTA was purified from L. plantarum
through butanol extraction followed by hydrophobic
and ion-exchange chromatography. E. faecalis biofilms were
formed over 3 weeks on glass bottom dishes and in dentin
blocks obtained from human single-rooted premolars. These
mature biofilms were treated with or without Lp.LTA for 1 h,
followed by additional treatment with either chlorhexidine
digluconate (CHX), calcium hydroxide (CH), or triple antibiotics
for 24 h. Biofilms on glass were live/dead stained and
quantified by ZEN through confocal laser microscopy. Biofilms
in dentin were fixed, sputter coated and analyzed by
ImageJ with scanning electron microscopy. Preformed E. faecalis
mature biofilms on the culture dishes were dose-dependently
disrupted by Lp.LTA. Lp.LTA potentiated the effects
of CHX or CH on the disruption of mature biofilm. Interestingly,
CHX-induced disruption of preformed E. faecalis
mature biofilms was synergistically enhanced only when pretreated
with Lp.LTA. Furthermore, in the dentin block model,
Lp.LTA alone reduced E. faecalis mature biofilm and
pre-treatment with Lp.LTA promoted the anti-biofilm activity
of CHX. Lp.LTA could be an anti-biofilm or supplementary
agent that can be effective for E. faecalis-biofilminduced
diseases.
-
Citations
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- Lipoteichoic acid from a canine probiotic strain Lacticaseibacillus rhamnosus possesses anti-biofilm capacity against clinically isolated canine periodontopathic Porphyromonas species
Somin Park, Jeongmin Shin, Woohyung Jun, Dongwook Lee, San Kim, Dong Uk Ha, Jintaek Im, Seung Hyun Han
Microbial Pathogenesis.2025; 205: 107660. CrossRef - Optimization of the Purification Process for Lactiplantibacillus plantarum Lipoteichoic Acid with Anti-Biofilm Properties against Dental Pathogens
Jeongmin Shin, Dong Hyun Park, Woohyung Jun, Ok-Jin Park, Cheol-Heui Yun, Jintaek Im, Seung Hyun Han
Journal of Microbiology and Biotechnology.2025;[Epub] CrossRef - Antibacterial efficacy of ultrasonically activated probiotic endodontic irrigant against Enterococcus faecalis biofilm: an in-vitro study
Mai Sayed Hanafy, Noha Mohamed Kamal, Hebatallah Atef Fathallah
BMC Oral Health.2025;[Epub] CrossRef - A Systematic Review of the Comparative Efficacy of Lactobacillus Probiotics and Sodium Hypochlorite as Intracanal Irrigants Against Enterococcus faecalis
Mrinalini Mrinalini, Alpa Gupta, Dax Abraham, Arun Kumar Duraisamy, Rajat Sharma
Cureus.2024;[Epub] CrossRef -
The role of
Lactobacillus plantarum
in oral health: a review of current studies
Xinyan Huang, Jianhang Bao, Mingzhen Yang, Yingying Li, Youwen Liu, Yuankun Zhai
Journal of Oral Microbiology.2024;[Epub] CrossRef - Lipoteichoic Acid from Lacticaseibacillus rhamnosus GG as a Novel Intracanal Medicament Targeting Enterococcus faecalis Biofilm Formation
Ji-Young Yoon, Somin Park, Dongwook Lee, Ok-Jin Park, WooCheol Lee, Seung Hyun Han
Journal of Microbiology.2024; 62(10): 897. CrossRef - Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT
Chengran Guan, Feng Li, Peng Yu, Xuan Chen, Yongqi Yin, Dawei Chen, Ruixia Gu, Chenchen Zhang, Bo Pang
Foods.2024; 13(17): 2706. CrossRef - Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
Prerna Saini, Repally Ayyanna, Rishi Kumar, Sayan Kumar Bhowmick, Vinay Bhaskar, Bappaditya Dey
Frontiers in Microbiology.2024;[Epub] CrossRef - Enterococcus Phage vB_EfaS_HEf13 as an Anti-Biofilm Agent Against Enterococcus faecalis
Dongwook Lee, Jintaek Im, A Reum Kim, Woohyung Jun, Cheol-Heui Yun, Seung Hyun Han
Journal of Microbiology.2024; 62(8): 683. CrossRef - Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
Shymaa Shaaban, Salma Genena, Alaaeldin Elraggal, Gamal M. Hamad, Marwa A. Meheissen, Sybel Moussa
BMC Oral Health.2023;[Epub] CrossRef - Antioxidant Effects and Probiotic Properties of Latilactobacillus sakei MS103 Isolated from Sweet Pickled Garlic
Heng Li, Changlin Chen, Yuanxin Li, Zhengqiang Li, Chen Li, Chang Luan
Foods.2023; 12(23): 4276. CrossRef - Effectiveness of probiotics in apical periodontitis progression: A scoping review and implications for research
Giusy Rita Maria La Rosa, Eugenio Pedullà
Australian Endodontic Journal.2023; 49(S1): 528. CrossRef - Antibacterial efficacy of antibiotic pastes versus calcium hydroxide intracanal dressing: A systematic review and meta-analysis of ex vivo studies
Mohammadreza Vatankhah, Kamyar Khosravi, Nazanin Zargar, Armin Shirvani, MohammadHossein Nekoofar, Omid Dianat
Journal of Conservative Dentistry.2022; 25(5): 463. CrossRef - Plantaricin LD1 purified from Lactobacillus plantarum LD1 inhibits biofilm formation of Enterococcus faecalis ATCC 29212 in tooth model
M.K. Yadav, P. Yadav, M. Dhiman, S. Tewari, S.K. Tiwari
Letters in Applied Microbiology.2022; 75(3): 623. CrossRef - The Products of Probiotic Bacteria Effectively Treat Persistent Enterococcus faecalis Biofilms
Shatha Safadi, Harsh Maan, Ilana Kolodkin-Gal, Igor Tsesis, Eyal Rosen
Pharmaceutics.2022; 14(4): 751. CrossRef - Regenerative Endodontics and Minimally Invasive Dentistry: Intertwining Paths Crossing Over Into Clinical Translation
Hisham Elnawam, Menatallah Abdelmougod, Ahmed Mobarak, Mai Hussein, Hamdy Aboualmakarem, Michael Girgis, Rania El Backly
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Can Probiotics Emerge as Effective Therapeutic Agents in Apical Periodontitis? A Review
Gaurav Kumar, Sanjay Tewari, John Tagg, Michael Leonidas Chikindas, Igor V Popov, Santosh Kumar Tiwari
Probiotics and Antimicrobial Proteins.2021; 13(2): 299. CrossRef - Interplay between Candida albicans and Lactic Acid Bacteria in the Gastrointestinal Tract: Impact on Colonization Resistance, Microbial Carriage, Opportunistic Infection, and Host Immunity
Karen D. Zeise, Robert J. Woods, Gary B. Huffnagle
Clinical Microbiology Reviews.2021;[Epub] CrossRef - Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis
M. Bertolini, R. Vazquez Munoz, L. Archambault, S. Shah, J. G. S. Souza, R. C. Costa, A. Thompson, Y. Zhou, T. Sobue, A. Dongari-Bagtzoglou, Deborah A. Hogan
mBio.2021;[Epub] CrossRef - Gram Positive Bacterial Lipoteichoic Acid Role in a Root Canal Infection – A Literature Review
Vinoo Subramaniam Ramachandran, Mensudar Rathakrishnan, Malathy Balaraman Ravindrran, Alargarsamy Venkatesh, Vidhya Shankari Shanmugasundaram, Karpagavinayagam Kumaraguru
Journal of Pure and Applied Microbiology.2021; 15(2): 534. CrossRef - Lactobacillus plantarum Lipoteichoic Acids Possess Strain-Specific Regulatory Effects on the Biofilm Formation of Dental Pathogenic Bacteria
Dongwook Lee, Jintaek Im, Dong Hyun Park, Sungho Jeong, Miri Park, Seokmin Yoon, Jaewoong Park, Seung Hyun Han
Frontiers in Microbiology.2021;[Epub] CrossRef - Streptococcus gordonii: Pathogenesis and Host Response to Its Cell Wall Components
Ok-Jin Park, Yeongkag Kwon, Chaeyeon Park, Yoon Ju So, Tae Hwan Park, Sungho Jeong, Jintaek Im, Cheol-Heui Yun, Seung Hyun Han
Microorganisms.2020; 8(12): 1852. CrossRef
Review
- [MINIREVIEW] New perspectives of Lactobacillus plantarum as a probiotic: The gut-heart-brain axis
-
Yen-Wenn Liu , Min-Tze Liong , Ying-Chieh Tsai
-
J. Microbiol. 2018;56(9):601-613. Published online August 23, 2018
-
DOI: https://doi.org/10.1007/s12275-018-8079-2
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707
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120
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Abstract
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Lactobacillus plantarum is a non-gas-producing lactic acid
bacterium that is generally regarded as safe (GRAS) with
Qualified Presumption of Safety (QPS) status. Although traditionally
used for dairy, meat and vegetable fermentation,
L. plantarum is gaining increasing significance as a probiotic.
With the newly acclaimed gut-heart-brain axis, strains of L.
plantarum have proven to be a valuable species for the development
of probiotics, with various beneficial effects on gut
health, metabolic disorders and brain health. In this review,
the classification and taxonomy, and the relation of these
with safety aspects are introduced. Characteristics of L. plantarum
to fulfill the criteria as a probiotic are discussed. Emphasis
are also given to the beneficial functions of L. plantarum
in gut disorders such as inflammatory bowel diseases,
metabolic syndromes, dyslipidemia, hypercholesteromia, obesity,
and diabetes, and brain health aspects involving psychological
disorders.
-
Citations
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Lactiplantibacillus plantarum
as a promising adjuvant for neurological disorders therapy through the brain-gut axis and related action pathways
Josilaene Duarte Luiz, Cynthia Manassi, Marciane Magnani, Adriano Gomes da Cruz, Tatiana Colombo Pimentel, Silvani Verruck
Critical Reviews in Food Science and Nutrition.2025; 65(4): 715. CrossRef - Single and repeated-dose toxicity studies by intravaginal administration of Lactobacillus plantarum ATG-K2 powder in female rats
Jae-Hyun Kang, Min-Soo Kang, Sun-Don Kim, Hyun-Kul Lee, Si-Whan Song, Chun-Ja Nam, Kwang-Il Park
Toxicological Research.2025; 41(1): 27. CrossRef - Metal ion-induced multi-layer coatings improves gastrointestinal resistance of Lactiplantibacillus plantarum and biosafety evaluation
Yanming Ren, Shuifang Mao, Pin Chen, Xingqian Ye, Jinhu Tian
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Food and Chemical Toxicology.2022; 167: 113308. CrossRef - Evaluation of the Probiotic In Vitro Potential of Lactic Acid-Producing Bacteria from Canine Vagina: Possible Role in Vaginal Health
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Preventive Nutrition and Food Science.2022; 27(1): 1. CrossRef - Lactiplantibacillus plantarum ST-III-fermented milk improves autistic-like behaviors in valproic acid-induced autism spectrum disorder mice by altering gut microbiota
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Frontiers in Nutrition.2022;[Epub] CrossRef - In Vitro Assessment of Bio-Functional Properties from Lactiplantibacillus plantarum Strains
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Journal of Parenteral and Enteral Nutrition.2022; 46(8): 1932. CrossRef - In Vitro and In Vivo Assessments of Anti-Hyperglycemic Properties of Soybean Residue Fermented with Rhizopus oligosporus and Lactiplantibacillus plantarum
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International Journal of Environmental Research and Public Health.2022; 19(24): 16549. CrossRef - Hypolipidemic and hypoglycemic nature of lactobacillus strains in fermented vegetable and dairy products
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Frontiers in Food Science and Technology.2022;[Epub] CrossRef - Mathematical Modeling and Optimization of Lactobacillus Species Single and Co-Culture Fermentation Processes in Wheat and Soy Dough Mixtures
Eva-H. Dulf, Dan C. Vodnar, Alex Danku, Adrian Gheorghe Martău, Bernadette-Emőke Teleky, Francisc V. Dulf, Mohamed Fawzy Ramadan, Ovidiu Crisan
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Mechanisms Underlying the Interaction Between Chronic Neurological Disorders and Microbial Metabolites via Tea Polyphenols Therapeutics
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Frontiers in Microbiology.2022;[Epub] CrossRef - Probiotics Reduce Vaginal Candidiasis in Pregnant Women via Modulating Abundance of Candida and Lactobacillus in Vaginal and Cervicovaginal Regions
Xin Ang, Uma Mageswaran, Yi Chung, Boon Lee, Siti Azhar, Nurhanis Roslan, Ili Saufian, Nor Mustaffa, Ermadina Kalam, Aini Ibrahim, Normala Abdul Wahid, Zakuan Deris, Chern-Ein Oon, Wan Adnan, Salina Sany, Min-Tze Liong
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Applied Sciences.2022; 12(19): 9983. CrossRef - Heart Failure Severity Closely Correlates with Intestinal Dysbiosis and Subsequent Metabolomic Alterations
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Journal of Pharmaceutical and Biomedical Analysis.2022; 217: 114843. CrossRef - Camellia (Camellia oleifera bel.) seed oil reprograms gut microbiota and alleviates lipid accumulation in high fat-fed mice through the mTOR pathway
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Food & Function.2022; 13(9): 4977. CrossRef - Long-Chain Acylcholines Link Butyrylcholinesterase to Regulation of Non-neuronal Cholinergic Signaling
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Food Science of Animal Resources.2022; 42(6): 1031. CrossRef - Genomic and Phenotypic Evaluation of Potential Probiotic Pediococcus Strains with Hypocholesterolemic Effect Isolated from Traditional Fermented Food
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Probiotics and Antimicrobial Proteins.2022; 14(6): 1042. CrossRef - Antibacterial Activity of Lactobacillus plantarum Supernatant on Non-Fermenting Gram-Negative Bacteria
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Journal Articles
- Functional expression and enzymatic characterization of Lactobacillus plantarum cyclomaltodextrinase catalyzing novel acarbose hydrolysis
-
Myoung-Uoon Jang , Hye-Jeong Kang , Chang-Ku Jeong , Yewon Kang , Ji-Eun Park , Tae-Jip Kim
-
J. Microbiol. 2018;56(2):113-118. Published online February 2, 2018
-
DOI: https://doi.org/10.1007/s12275-018-7551-3
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382
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9
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Abstract
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Cyclomaltodextrinases (CDases) belong to Glycoside Hydrolases
(GH) family 13, which show versatile hydrolyzing
and/or transglycosylation activity against cyclodextrin (CD),
starch, and pullulan. Especially, some CDases have been reported
to hydrolyze acarbose, a potent α-glucosidase inhibitor,
and transfer the resulting acarviosine-glucose to various
acceptors. In this study, a novel CDase (LPCD) gene was
cloned from Lactobacillus plantarum WCFS1, which encodes
574 amino acids (64.6 kDa) and shares less than 44% of identities
with the known CDase-family enzymes. Recombinant
LPCD with C-terminal six-histidines was produced and purified
from Escherichia coli. It showed the highest activity
on β-CD at 45°C and pH 5.0, respectively. Gel permeation
chromatography analysis revealed that LPCD exists as a dodecameric
form (~826 kDa). Its hydrolyzing activity on β-
CD is almost same as that on starch, whereas it can hardly
attack pullulan. Most interestingly, LPCD catalyzed the unique
modes of action in acarbose hydrolysis to produce maltose
and acarviosine, as well as to glucose and acarviosineglucose.
-
Citations
Citations to this article as recorded by

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Genetic and enzymatic characterization of Amy13E from
Cellvibrio japonicus
reclassifies it as a cyclodextrinase also capable of α-diglucoside degradation
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Applied and Environmental Microbiology.2024;[Epub] CrossRef - Food-grade expression and characterization of cyclomaltodextrinase from B. sphaericus E−244 in Bacillus subtilis
Ruiqi Zhou, Luhua Zheng, Bo Jiang, Weiwei He, Ran Zhang, Jingjing Chen, Assam Bin Tahir
Food Bioscience.2024; 61: 104726. CrossRef - Enhancement of the structure and biochemical function of cyclomaltodextrinase from the Anoxybacillus flavithermus ZNU-NGA with site-directed mutagenesis
Ziba Mirzaee, Vahab Jafarian, Khosrow Khalifeh
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- Effects of heat-killed Lactobacillus plantarum against influenza viruses in mice
-
Sehee Park , Jin Il Kim , Joon-Yong Bae , Kirim Yoo , Hyunung Kim , In-Ho Kim , Man-Seong Park , Ilseob Lee
-
J. Microbiol. 2018;56(2):145-149. Published online February 2, 2018
-
DOI: https://doi.org/10.1007/s12275-018-7411-1
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439
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31
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Abstract
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The potential use of dietary measures to treat influenza can
be an important alternative for those who lack access to influenza
vaccines or antiviral drugs. Lactobacillus plantarum
(Lp) is one of many lactic acid bacteria that grow in ‘kimchi’,
an essential part of Korean meal, and several strains of Lp
reportedly show protective effects against influenza. Using
heat-killed Lp (nF1) isolated from kimchi, which is known
for its immunomodulatory effects, we investigated whether
regular oral intake of nF1 could influence the outcome of influenza
virus infection in a mouse model. In a lethal challenge
with influenza A (H1N1 and H3N2 subtypes) and influenza
B (Yamagata lineage) viruses, daily oral administration
of nF1 delayed the mean number of days to death of the
infected mice and resulted in increased survival rates compared
with those of the non-treated mice. Consistent with
these observations, nF1 treatment also significantly reduced
viral replication in the lungs of the infected mice. Taken together,
our results might suggest the remedial potential of heatkilled
Lactobacillus probiotics against influenza.
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- Probiotic potential of novel Lactobacillus strains isolated from salted-fermented shrimp as antagonists for Vibrio parahaemolyticus
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Bao Le , Seung Hwan Yang
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J. Microbiol. 2018;56(2):138-144. Published online February 2, 2018
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DOI: https://doi.org/10.1007/s12275-018-7407-x
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387
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Abstract
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Lactobacillus strains have been considered good candidates
as biological control agents for prevention or treatment of
plant and animal infections. One L. plantarum strain FB003
and three strains (FB011, FB081, and FB110) which closed
to L. sakei were isolated from fermented and salted shrimp
and their abilities in inhibiting growth of Vibrio parahaemolyticus
were characterized. These strains were selected as
potential probiotics based on their oro-gastro-intestinal resistance,
gut colonization, adhesion to Caco-2 cells, antimicrobial
activities, antibiotic resistance, and safety aspects.
Results
of this study revealed that these isolates possessed high
aggregation activities against pathogens in host intestines.
Strain FB011 strain showed higher coaggregation and immunomodulatory
activity in the gastro-intestinal tract than
L. plantarum. These difference effects of Lactobacillus strains
provide valuable information about using them to prevent
Vibrio infections in the aquaculture industry.
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saeujeot
)
Ju-Young Lim, Yun-Jeong Choi, Seong Youl Lee, Min Jung Lee, Hae-Il Yang, Eun-Hae Kim, Sung Jin Park, Ji-Hee Yang, Young Bae Chung, Sung-Hee Park, Sung Gi Min, Mi-Ai Lee
International Journal of Food Properties.2022; 25(1): 2332. CrossRef - Isolation and Characterization of a Bacillus velezensis D-18 Strain, as a Potential Probiotic in European Seabass Aquaculture
Luis Monzón-Atienza, Jimena Bravo, Silvia Torrecillas, Daniel Montero, Ana Franco González-de Canales, Inés. García de la Banda, Jorge Galindo-Villegas, José Ramos-Vivas, Félix Acosta
Probiotics and Antimicrobial Proteins.2021; 13(5): 1404. CrossRef - Shrimp disease management for sustainable aquaculture: innovations from nanotechnology and biotechnology
P.S Seethalakshmi, Riya Rajeev, George Seghal Kiran, Joseph Selvin
Aquaculture International.2021; 29(4): 1591. CrossRef -
Anti‐biofilm potential of kefir‐derived
Lactobacillus paracasei
L10 against
Vibrio parahaemolyticus
W. Shangguan, T. Xie, R. Zhang, C. Lu, X. Han, Q. Zhong
Letters in Applied Microbiology.2021; 73(6): 750. CrossRef - Vibrio and major commercially important vibriosis diseases in decapod crustaceans
Cecília de Souza Valente, Alex H.L. Wan
Journal of Invertebrate Pathology.2021; 181: 107527. CrossRef - Using lactic acid bacteria as an immunostimulants in cultured shrimp with special reference to Lactobacillus spp.
Mohammed A. E. Naiel, Mayada R. Farag, Ahmed G. A. Gewida, Mahmoud A. Elnakeeb, Mahmoud S. Amer, Mahmoud Alagawany
Aquaculture International.2021; 29(1): 219. CrossRef - In Silico Prediction of Novel Probiotic Species Limiting Pathogenic Vibrio Growth Using Constraint-Based Genome Scale Metabolic Modeling
Neelakantan Thulasi Devika, Ashok Kumar Jangam, Vinaya Kumar Katneni, Prasanna Kumar Patil, Suganya Nathamuni, Mudagandur Shashi Shekhar
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - In vitro antagonistic effect and in vivo protective efficacy of Gram-positive probiotics versus Gram-negative bacterial pathogens in finfish and shellfish
Hien Van Doan, Mehdi Soltani, Einar Ringø
Aquaculture.2021; 540: 736581. CrossRef - Isolation, Screening, and Identification of Proteolytic Lactic Acid Bacteria from IndigenousChaoProduct
Agussalim Matti, Tyas Utami, Chusnul Hidayat, Endang S. Rahayu
Journal of Aquatic Food Product Technology.2019; 28(7): 781. CrossRef - The marine symbiont Pseudovibrio denitrificans, is effective to control pathogenic Vibrio spp. in shrimp aquaculture
Cristóbal Domínguez-Borbor, Valeska Ardiles, Marissa Bermeo, Chalén Bolívar-Alvarado, Cecilia Tomalá, Stanislaus Sonnenholzner, Jenny A. Rodríguez
Aquaculture.2019; 508: 127. CrossRef
- Comparison of antibiotic resistance and copper tolerance of Enterococcus spp. and Lactobacillus spp. isolated from piglets before and after weaning
-
Xueting Zou , Mengwei Weng , Xu Ji , Rong Guo , Weijiang Zheng , Wen Yao
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J. Microbiol. 2017;55(9):703-710. Published online September 2, 2017
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DOI: https://doi.org/10.1007/s12275-017-6241-x
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360
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7
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Abstract
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In China, antimicrobials and copper are used extensively as
growth-promoting agents for piglets. This study aimed to
characterize the role of in-feed copper in the emergence of
copper-tolerant and antibiotic-resistant Enterococcus and
Lactobacillus isolates in Chinese pig farms. Feces of the same
eight piglets from four litters at 7 and 55 days old and their
mothers were traced in order to isolate Enterococcus spp.
and Lactobacillus spp.. The minimum inhibitory concentrations
of 10 antimicrobials and copper sulfate were determined
using an agar dilution method. The feed levels of Cu2+ for
lactating sows, suckling piglets, and weaned piglets were 6,
177, and 18 mg/kg, respectively. All the 136 Enterococcus isolates
were sensitive to vancomycin; and the resistance rates
to penicillin, enrofloxacin, and high level streptomycin resistance
increased significantly after weaning. For the 155 Lactobacillus
isolates, the resistance rates to ampicillin, chloramphenicol,
tetracycline, and enrofloxacin were significantly
higher in weaned piglets. The ratios of copper tolerant Enterococcus
and Lactobacillus isolates both increased significantly
after weaning (P < 0.05). A phenotypic correlation was observed
after classifying the isolates into two groups (CuSO4
MIC50 < 16 or 16 for enterococci; CuSO4 MIC50 < 12 or 12 for lactobacilli) and comparing the antimicrobial-resistant
percentage of two groups. On species level, a significant
increase of E. faecalis to enrofloxacin was observed in
line with the increase of copper MIC (P < 0.05). The findings
revealed the changes of the antibiotic resistance and copper
tolerance level of enterococci and lactobacilli between suckling
and weaned piglets and demonstrated that there might
be a strong association between in-feed copper and increased
antibiotic resistance in enterococci and lactobacilli in Chinese
intensive swine farms.
-
Citations
Citations to this article as recorded by

- Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic
Katerina Kavanova, Iveta Kostovova, Monika Moravkova, Tereza Kubasova, Vladimir Babak, Magdalena Crhanova
Probiotics and Antimicrobial Proteins.2025; 17(4): 1820. CrossRef - Copper Stress Response of Antibiotic‐Resistant and Copper‐Tolerant Enterococcus faecium and the Antibacterial Efficacy of Functionalized Copper Surfaces
Franca Arndt, Katharina Siems, Stella Marie Timofeev, Philippe Baumann, Aisha Saddiqa Ahmed, Daniel Wyn Müller, Frank Mücklich, Aaron Haben, Ralf Kautenburger, Ronja Anton, Christoph Schaudinn, Michael Laue, Oliver Schwengers, Katharina Runzheimer, Rocío
Advanced Materials Interfaces.2025;[Epub] CrossRef - Uncovering the effects of copper feed supplementation on the selection of copper-tolerant and antibiotic-resistant Enterococcus in poultry production for sustainable environmental practices
Andreia Rebelo, Bárbara Duarte, Ana R. Freitas, Agostinho Almeida, Rui Azevedo, Edgar Pinto, Luísa Peixe, Patrícia Antunes, Carla Novais
Science of The Total Environment.2023; 900: 165769. CrossRef - Effect of Dietary Fructus mume and Scutellaria baicalensis Georgi on the Fecal Microbiota and Its Correlation with Apparent Nutrient Digestibility in Weaned Piglets
Feng Zhang, Erhui Jin, Xiaodan Liu, Xu Ji, Hong Hu
Animals.2022; 12(18): 2418. CrossRef - Antibiotic Susceptibility, Resistance Gene Determinants and Corresponding Genomic Regions in Lactobacillus amylovorus Isolates Derived from Wild Boars and Domestic Pigs
Monika Moravkova, Iveta Kostovova, Katerina Kavanova, Radko Pechar, Stanislav Stanek, Ales Brychta, Michal Zeman, Tereza Kubasova
Microorganisms.2022; 11(1): 103. CrossRef - Lactobacillus fermentum and Lactobacillus plantarum bioremediation ability assessment for copper and zinc
Saeed Hasr Moradi Kargar, Najmeh Hadizadeh Shirazi
Archives of Microbiology.2020; 202(7): 1957. CrossRef - Selection and dissemination of antimicrobial resistance in Agri-food production
Guyue Cheng, Jianan Ning, Saeed Ahmed, Junhong Huang, Rizwan Ullah, Boyu An, Haihong Hao, Menghong Dai, Lingli Huang, Xu Wang, Zonghui Yuan
Antimicrobial Resistance & Infection Control.2019;[Epub] CrossRef
- Intestinal Lactobacillus community structure and its correlation with diet of Southern Chinese elderly subjects§
-
Yuanyuan Pan , Da-Wen Sun , Quanyang Li
-
J. Microbiol. 2016;54(9):594-601. Published online August 31, 2016
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DOI: https://doi.org/10.1007/s12275-016-6131-7
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365
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8
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Abstract
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This study aimed to investigate the relationship between the
intestinal Lactobacillus species and diet of elderly subjects in
a longevity area in Southern China. Healthy elderly subjects
ranging from 80 to 99 years old were respectively selected
from the regions of Bama and Nanning, Guangxi, China.
The nested polymerase chain reaction and denaturing gradient
gel electrophoresis (PCR-DGGE) technology was used
to analyze the intestinal Lactobacillus community structure.
Results
showed that Weissella confusa, L. mucosae, L. crispatus,
L. salivarius, and L. delbrueckii were the representative
Lactobacillus of elderly subjects. Among them, L. crispatus
and L. delbrueckii were the dominant Lactobacillus of
all species. In comparison to Nanning elderly subjects, the
detection frequencies of W. confusa and L. salivarius were
significantly increased in Bama elderly subjects (P < 0.01),
whereas L. mucosae was significantly decreased (P < 0.01).
Interestingly, it was also found that there were 4 kinds of
representative Lactobacillus, which were significantly correlated
with dietary fiber. W. confusa (P < 0.01) and L. salivarius
(P < 0.05) were significantly positively correlated with
the intake of dietary fiber, while L. mucosae (P < 0.01) and
L. crispatus (P < 0.05) were significantly negatively correlated
with the intake of dietary fiber, respectively. Results confirmed
that different diets had obvious effects on the intestinal Lactobacillus
community structure of elderly subjects in Southern
China, which may provide a certain theoretical basis for the
elderly’s healthy food strategic design and probiotics product
development.
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Citations
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- Metabolomic and Transcriptomic Analyses Provide New Insights into Health-Promoting Metabolites from Cannabis Seeds Growing in the Bama Region of China
Mingzheng Duan, Muhammad Junaid Rao, Qing Li, Falin Zhao, Hongzeng Fan, Bo Li, Dandan He, Shijian Han, Jiangjiang Zhang, Lingqiang Wang
Agronomy.2024; 14(4): 787. CrossRef - Which Factors Influence Healthy Aging? A Lesson from the Longevity Village of Bama in China
Wei Zhang, Qingyun Huang, Yongxin Kang, Hao Li, Guohe Tan
Aging and disease.2023; 14(3): 825. CrossRef - Widely targeted metabolomics analysis reveals the major metabolites in the hemp seeds from the longevity village of Bama, China
Zhongting Chen, Shuxia Hao, Zhaoyuan He, Jing Liu, Jin Zhao, Chunrong Chen, Gengjie Jia, Hailan Chen
Industrial Crops and Products.2023; 206: 117661. CrossRef - Centenarian-Sourced Lactobacillus casei Combined with Dietary Fiber Complex Ameliorates Brain and Gut Function in Aged Mice
Minhong Ren, He Li, Zhen Fu, Quanyang Li
Nutrients.2022; 14(2): 324. CrossRef - Effect of probiotics on gingival inflammation and oral microbiota: A meta‐analysis
Jiaxuan Liu, Zhenmin Liu, Jiaqi Huang, Renchuan Tao
Oral Diseases.2022; 28(4): 1058. CrossRef - The Gut Microbiome, Aging, and Longevity: A Systematic Review
Varsha D. Badal, Eleonora D. Vaccariello, Emily R. Murray, Kasey E. Yu, Rob Knight, Dilip V. Jeste, Tanya T. Nguyen
Nutrients.2020; 12(12): 3759. CrossRef - Inactivation of Listeria Monocytogenes at various growth temperatures by ultrasound pretreatment and cold plasma
Yuanyuan Pan, Yan Zhang, Jun-Hu Cheng, Da-Wen Sun
LWT.2020; 118: 108635. CrossRef - Literature-based safety assessment of an agriculture- and animal-associated microorganism: Weissella confusa
Joseph M. Sturino
Regulatory Toxicology and Pharmacology.2018; 95: 142. CrossRef
- Carbohydrate-binding specificities of potential probiotic Lactobacillus strains in porcine jejunal (IPEC-J2) cells and porcine mucin
-
Valerie Diane Valeriano , Bernadette B. Bagon , Marilen P. Balolong , Dae-Kyung Kang
-
J. Microbiol. 2016;54(7):510-519. Published online June 28, 2016
-
DOI: https://doi.org/10.1007/s12275-016-6168-7
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420
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24
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Abstract
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Bacterial lectins are carbohydrate-binding adhesins that recognize
glycoreceptors in the gut mucus and epithelium of
hosts. In this study, the contribution of lectin-like activities
to adhesion of Lactobacillus mucosae LM1 and Lactobacillus
johnsonii PF01, which were isolated from swine intestine,
were compared to those of the commercial probiotic Lactobacillus
rhamnosus GG. Both LM1 and PF01 strains have
been reported to have good adhesion ability to crude intestinal
mucus of pigs. To confirm this, we quantified their adhesion
to porcine gastric mucin and intestinal porcine enterocytes
isolated from the jejunum of piglets (IPEC-J2). In addition,
we examined their carbohydrate-binding specificities by suspending
bacterial cells in carbohydrate solutions prior to adhesion
assays. We found that the selected carbohydrates affected
the adherences of LM1 to IPEC-J2 cells and of LGG to
mucin. In addition, compared to adhesion to IPEC-J2 cells,
adhesion to mucin by both LM1 and LGG was characterized
by enhanced specific recognition of glycoreceptor components
such as galactose, mannose, and N-acetylglucosamine.
Hydrophobic interactions might make a greater contribution
to adhesion of PF01. A similar adhesin profile between a probiotic
and a pathogen, suggest a correlation between shared
pathogen–probiotic glycoreceptor recognition and the ability
to exclude enteropathogens such as Escherichia coli K88 and
Salmonella Typhimurium KCCM 40253. These findings extend
our understanding of the mechanisms of the intestinal
adhesion and pathogen-inhibition abilities of probiotic Lactobacillus
strains.
-
Citations
Citations to this article as recorded by

- Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic
Katerina Kavanova, Iveta Kostovova, Monika Moravkova, Tereza Kubasova, Vladimir Babak, Magdalena Crhanova
Probiotics and Antimicrobial Proteins.2025; 17(4): 1820. CrossRef - Effects of Probiotics on Gut Microbiota: An Overview
Preethi Chandrasekaran, Sabine Weiskirchen, Ralf Weiskirchen
International Journal of Molecular Sciences.2024; 25(11): 6022. CrossRef - Safety assessment of five candidate probiotic lactobacilli using comparative genome analysis
Patrick Josemaria d.R Altavas, Mia Beatriz C. Amoranto, Sang Hoon Kim, Dae-Kyung Kang, Marilen P. Balolong, Leslie Michelle M. Dalmacio
Access Microbiology
.2024;[Epub] CrossRef - Galacto-oligosaccharides regulate intestinal mucosal sialylation to counteract antibiotic-induced mucin dysbiosis
Laipeng Xu, Xuan Li, Shuibing Han, Chunlong Mu, Weiyun Zhu
Food & Function.2024; 15(24): 12016. CrossRef - Isolation and Characterization of Lactic Acid Bacteria With Probiotic Attributes From Different Parts of the Gastrointestinal Tract of Free-living Wild Boars in Hungary
Tibor Keresztény, Balázs Libisch, Stephanya Corral Orbe, Tibor Nagy, Zoltán Kerényi, Róbert Kocsis, Katalin Posta, Péter P. Papp, Ferenc Olasz
Probiotics and Antimicrobial Proteins.2024; 16(4): 1221. CrossRef - The Effects of Cellular Membrane Damage on the Long-Term Storage and Adhesion of Probiotic Bacteria in Caco-2 Cell Line
Jakub Kiepś, Wojciech Juzwa, Anna Olejnik, Anna Sip, Jolanta Tomaszewska-Gras, Radosław Dembczyński
Nutrients.2023; 15(15): 3484. CrossRef - Targeting gut microbiota and metabolism as the major probiotic mechanism - An evidence-based review
Teng Ma, Xin Shen, Xuan Shi, Hafiz Arbab Sakandar, Keyu Quan, Yalin Li, Hao Jin, Lai-Yu Kwok, Heping Zhang, Zhihong Sun
Trends in Food Science & Technology.2023; 138: 178. CrossRef - Difference analysis of intestinal microbiota and metabolites in piglets of different breeds exposed to porcine epidemic diarrhea virus infection
Zhili Li, Wandi Zhang, Langju Su, Zongyang Huang, Weichao Zhang, Liangliang Ma, Jingshuai Sun, Jinyue Guo, Feng Wen, Kun Mei, Saeed El-Ashram, Shujian Huang, Yunxiang Zhao
Frontiers in Microbiology.2022;[Epub] CrossRef - The effects of diet and gut microbiota on the regulation of intestinal mucin glycosylation
Dingwu Qu, Gang Wang, Leilei Yu, Fengwei Tian, Wei Chen, Qixiao Zhai
Carbohydrate Polymers.2021; 258: 117651. CrossRef - Immunomodulatory potential of four candidate probiotic Lactobacillus strains from plant and animal origin using comparative genomic analysis
Paul Benedic U. Salvador, Leslie Michelle M. Dalmacio, Sang Hoon Kim, Dae-Kyung Kang, Marilen P. Balolong
Access Microbiology
.2021;[Epub] CrossRef - Exoproteome Perspective on the Bile Stress Response of Lactobacillus johnsonii
Bernadette B. Bagon, Valerie Diane V. Valeriano, Ju Kyoung Oh, Edward Alain B. Pajarillo, Ji Yoon Lee, Dae-Kyung Kang
Proteomes.2021; 9(1): 10. CrossRef - Exploring the Bile Stress Response of Lactobacillus mucosae LM1 through Exoproteome Analysis
Bernadette B. Bagon, Ju Kyoung Oh, Valerie Diane V. Valeriano, Edward Alain B. Pajarillo, Dae-Kyung Kang
Molecules.2021; 26(18): 5695. CrossRef - Characterization of Lactic Acid Bacteria Isolated From the Gastrointestinal Tract of a Wild Boar as Potential Probiotics
Miao Li, Yi Wang, Hongyu Cui, Yongfeng Li, Yuan Sun, Hua-Ji Qiu
Frontiers in Veterinary Science.2020;[Epub] CrossRef - Characterisation of a lysophospholipase from Lactobacillus mucosae
Sang Hoon Kim, Ji Hoon Song, Jinyoung Kim, Dae-Kyung Kang
Biotechnology Letters.2020; 42(9): 1735. CrossRef - Quantifying and Engineering Mucus Adhesion of Probiotics
Zachary J. S. Mays, Todd C. Chappell, Nikhil U. Nair
ACS Synthetic Biology.2020; 9(2): 356. CrossRef - Immune boosting functional foods and their mechanisms: A critical evaluation of probiotics and prebiotics
Tolulope Joshua Ashaolu
Biomedicine & Pharmacotherapy.2020; 130: 110625. CrossRef - Bovine colostrum-driven modulation of intestinal epithelial cells for increased commensal colonisation
Sinead T. Morrin, Jonathan A. Lane, Mariarosaria Marotta, Lars Bode, Stephen D. Carrington, Jane A. Irwin, Rita M. Hickey
Applied Microbiology and Biotechnology.2019; 103(6): 2745. CrossRef - Probiotics interaction with foodborne pathogens: a potential alternative to antibiotics and future challenges
Murphy Lam Yim Wan, Stephen J. Forsythe, Hani El-Nezami
Critical Reviews in Food Science and Nutrition.2019; 59(20): 3320. CrossRef - Comparative genomic analysis of Lactobacillus mucosae LM1 identifies potential niche-specific genes and pathways for gastrointestinal adaptation
Valerie Diane V. Valeriano, Ju Kyoung Oh, Bernadette B. Bagon, Heebal Kim, Dae-Kyung Kang
Genomics.2019; 111(1): 24. CrossRef - Comparative exoproteome analyses of Lactobacillus spp. reveals species- and strain-specific proteins involved in their extracellular interaction and probiotic potential
Bernadette B. Bagon, Valerie Diane V. Valeriano, Ju Kyoung Oh, Edward Alain B. Pajarillo, Chun-Sung Cho, Dae-Kyung Kang
LWT.2018; 93: 420. CrossRef - Proteomic View of the Crosstalk between Lactobacillus mucosae and Intestinal Epithelial Cells in Co-culture Revealed by Q Exactive-Based Quantitative Proteomics
Edward Alain B. Pajarillo, Sang Hoon Kim, Valerie Diane Valeriano, Ji Yoon Lee, Dae-Kyung Kang
Frontiers in Microbiology.2017;[Epub] CrossRef - Probiotic roles ofLactobacillussp. in swine: insights from gut microbiota
V.D.V. Valeriano, M.P. Balolong, D.-K. Kang
Journal of Applied Microbiology.2017; 122(3): 554. CrossRef - Effect of apple extracts and selective polyphenols on the adhesion of potential probiotic strains of Lactobacillus gasseri R and Lactobacillus casei FMP
Tereza Volstatova, Petr Marsik, Vojtech Rada, Martina Geigerova, Jaroslav Havlik
Journal of Functional Foods.2017; 35: 391. CrossRef - Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins
A. Gunning, Devon Kavanaugh, Elizabeth Thursby, Sabrina Etzold, Donald MacKenzie, Nathalie Juge
International Journal of Molecular Sciences.2016; 17(11): 1854. CrossRef
- Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice
-
Sung-Gang Jo , Eui-Jeong Noh , Jun-Young Lee , Green Kim , Joo-Hee Choi , Mo-Eun Lee , Jung-Hee Song , Ji-Yoon Chang , Jong-Hwan Park
-
J. Microbiol. 2016;54(7):503-509. Published online June 28, 2016
-
DOI: https://doi.org/10.1007/s12275-016-6160-2
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-
449
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0
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-
54
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Abstract
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Probiotics such as lactobacilli and bifidobacteria have healthpromoting
effects by immune modulation. In the present
study, we examined the immunomodulatory properties of
Lactobacillus curvatus WiKim38, which was newly isolated
from baechu (Chinese cabbage) kimchi. The ability of L.
curvatus WiKim38 to induce cytokine production in bone
marrow-derived dendritic cells (BMDCs) was determined by
enzyme-linked immunosorbent assay. To evaluate the molecular
mechanisms underlying L. curvatus Wikim38-mediated
IL-10 production, Western blot analyses and inhibitor
assays were performed. Moreover, the in vivo anti-inflammatory
effects of L. curvatus WiKim38 were examined in a dextran
sodium sulfate (DSS)-induced colitis mouse model. L.
curvatus WiKim38 induced significantly higher levels of IL-
10 in BMDCs compared with that induced by LPS. NF-κB
and ERK were activated by L. curvatus WiKim38, and an
inhibitor assay revealed that these pathways were required
for L. curvatus WiKim38-induced production of IL-10 in
BMDCs. An in vivo experiment showed that oral administration
of L. curvatus WiKim38 increased the survival rate
of mice with DSS-induced colitis and improved clinical signs
and histopathological severity in colon tissues. Taken together,
these results indicate that L. curvatus Wikim38 may
have health-promoting effects via immune modulation, and
may thus be applicable for therapy of various inflammatory
diseases.
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Xiaopeng Cao, Hui Zhao, Zhimin Liang, Yi Cao, Min Min
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Research Support, Non-U.S. Gov'ts
- Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model
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Joo Yeon Kang , Do Kyung Lee , Nam Joo Ha , Hea Soon Shin
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J. Microbiol. 2015;53(11):796-803. Published online October 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-5302-2
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Abstract
-
Rotavirus is worldwide cause of severe gastroenteritis including
severe diarrhea and fatal dehydration in infants
and young children. There is an available vaccination program
for preventing rotavirus infection, but it has limits
and restrictions. Probiotics therapy could be an alternative
method
of antiviral prevention and modulation against rotavirus
infection. In this study, we screened the antiviral activity
of probiotic bacteria such as 3 Lactobacillus spp. and
14 Bifidobacterium spp. isolated from young Korean. Three
of the bacteria, Lactobacillus ruminis SPM0211, Bifidobacterium
longum SPM1205, and SPM1206, inhibited human
strain Wa rotavirus infection in Caco-2 cells. Furthermore,
these bacterial strains inhibited rotavirus replication in a
rotavirus-infected neonatal mouse model. To clarify the mechanism
of inhibition, we investigated gene expression of
Interferon (IFN)-signaling components and IFN-inducible
antiviral effectors. All 3 probiotics increased IFN-α and IFN-
β levels compared with the control. Gene expression of IFNsignaling
components and IFN-inducible antiviral effectors
also increased. Overall, these results indicate that L. ruminis
SPM0211, B. longum SPM1205 and 1206 efficiently inhibit
rotavirus replication in vitro and in vivo. Especially, the antiviral
effect of Lactobacillus ruminis SPM0211 is worthy of
notice. This is the first report of L. ruminis with antiviral activity.
Anti-rotaviral effects of the 3 probiotics are likely due
to their modulation of the immune response through promoting
type I IFNs, which are key regulators in IFN signaling
pathway.
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Heetae Lee, GwangPyo Ko
Gut Microbes.2017; 8(6): 616. CrossRef
- Relationships between the use of Embden Meyerhof pathway (EMP) or Phosphoketolase pathway (PKP) and lactate production capabilities of diverse Lactobacillus reuteri strains
-
Grégoire Burgé , Claire Saulou-Bérion , Marwen Moussa , Florent Allais , Violaine Athes , Henry-Eric Spinnler
-
J. Microbiol. 2015;53(10):702-710. Published online October 2, 2015
-
DOI: https://doi.org/10.1007/s12275-015-5056-x
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635
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0
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30
Crossref
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Abstract
-
The aims of this study is to compare the growth and glucose
metabolism of three Lactobacillus reuteri strains (i.e.
DSM 20016, DSM 17938, and ATCC 53608) which are lactic
acid bacteria of interest used for diverse applications such
as probiotics implying the production of biomass, or for the
production of valuable chemicals (3-hydroxypropionaldehyde,
3-hydroxypropionic acid, 1,3-propanediol). However, the
physiological diversity inside the species, even for basic metabolisms,
like its capacity of acidification or glucose metabolism,
has not been studied yet. In the present work, the
growth and metabolism of three strains representative of
the species diversity have been studied in batch mode. The
strains were compared through characterization of growth
kinetics and evaluation of acidification kinetics, substrate consumption
and product formation. The results showed significant
differences between the three strains which may be
explained, at least in part, by variations in the distribution
of carbon source between two glycolytic pathways during the
bacterial growth: the phosphoketolase or heterolactic pathway
(PKP) and the Embden-Meyerhof pathway (EMP). It was
also shown that, in the context of obtaining a large amount
of biomass, DSM 20016 and DSM 17938 strains were the
most effective in terms of growth kinetics. The DSM 17938
strain, which shows the more significant metabolic shift from
EMP to PKP when the pH decreases, is more effective for
lactate production.
-
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Merve Atasoy, Avelino Álvarez Ordóñez, Adam Cenian, Aleksandra Djukić-Vuković, Peter A Lund, Fatih Ozogul, Janja Trček, Carmit Ziv, Daniela De Biase
FEMS Microbiology Reviews.2024;[Epub] CrossRef -
Estimating the contribution of the porcine fecal core microbiota to metabolite production via mathematical modeling and
in vitro
fermentation
Salvatore Galgano, Helen Kettle, Andrew Free, Jos G. M. Houdijk, Vanni Bucci
mSystems.2024;[Epub] CrossRef - Solid‐state fermentation: Bioconversions and impacts on bioactive and nutritional compounds in oats
Stella Green, Graham T. Eyres, Dominic Agyei, Biniam Kebede
Comprehensive Reviews in Food Science and Food Safety.2024;[Epub] CrossRef - Characterization of nuvita biosearch center (NBC) isolated lactic acid bacteria strains from human origin and determination of growth kinetic profiles of selected cultures under bioreactor
Akif Emre Kavak, İnci Zent, Ezgi Metin Sağır, Gülistan Öncü, Feride İrem Şimşek
Annals of Microbiology.2024;[Epub] CrossRef - Assessment of autochthonous lactic acid bacteria as starter culture for improving traditional Chinese Dongbei Suancai fermentation
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LWT.2023; 178: 114615. CrossRef - Mannitol Production by Heterofermentative Lactic Acid Bacteria: a Review
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Applied Biochemistry and Biotechnology.2022; 194(6): 2762. CrossRef - Production of high-value added exopolysaccharide by biotherapeutic potential Lactobacillus reuteri strain
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Biochemical Engineering Journal.2022; 188: 108691. CrossRef - High-resolution structure of phosphoketolase from Bifidobacterium longum determined by cryo-EM single-particle analysis
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Journal of Structural Biology.2022; 214(2): 107842. CrossRef - In Silico Genomic and Metabolic Atlas of Limosilactobacillus reuteri DSM 20016: An Insight into Human Health
Paisleigh Smythe, Georgios Efthimiou
Microorganisms.2022; 10(7): 1341. CrossRef - Changes and machine learning-based prediction in quality characteristics of sliced Korean cabbage (Brassica rapa L. pekinensis) kimchi: Combined effect of nano-foamed structure film packaging and subcooled storage
So Yoon Park, Miran Kang, Suk-Min Yun, Jong-Bang Eun, Bo-Sung Shin, Ho Hyun Chun
LWT.2022; 171: 114122. CrossRef -
Acids produced by lactobacilli inhibit the growth of commensal
Lachnospiraceae
and S24-7 bacteria
Emma J. E. Brownlie, Danica Chaharlangi, Erin Oi-Yan Wong, Deanna Kim, William Wiley Navarre
Gut Microbes.2022;[Epub] CrossRef - Salinity enhances high optically active L-lactate production from co-fermentation of food waste and waste activated sludge: Unveiling the response of microbial community shift and functional profiling
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Struan James Reid, Robert Alexander Speers, Nik Willoughby, William Bain Lumsden, Dawn Louise Maskell
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Qiao Wang, Huan Li, Kai Feng, Jianguo Liu
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Jae-Jun Lee, Yun-Jeong Choi, Min Jung Lee, Sung Jin Park, Su Jin Oh, Ye-Rang Yun, Sung Gi Min, Hye-Young Seo, Sung-Hee Park, Mi-Ai Lee
Food Research International.2020; 136: 109591. CrossRef - Impact of the fermentation parameters pH and temperature on stress resilience of Lactobacillus reuteri DSM 17938
Armando Hernández, Christer U. Larsson, Radoslaw Sawicki, Ed W. J. van Niel, Stefan Roos, Sebastian Håkansson
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Microbial Cell Factories.2019;[Epub] CrossRef - Towards sustainability of lactic acid and poly-lactic acid polymers production
A. Djukić-Vuković, D. Mladenović, J. Ivanović, J. Pejin, L. Mojović
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Elleke F. Bosma, Jochen Forster, Alex Toftgaard Nielsen
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Armando Hernández Garcia, Anke M. Herrmann, Sebastian Håkansson
Process Biochemistry.2017; 55: 49. CrossRef - Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis
Aida Kalantari, Tao Chen, Boyang Ji, Ivan A. Stancik, Vaishnavi Ravikumar, Damjan Franjevic, Claire Saulou-Bérion, Anne Goelzer, Ivan Mijakovic
Frontiers in Microbiology.2017;[Epub] CrossRef - Novel molecular, structural and evolutionary characteristics of the phosphoketolases from bifidobacteria and Coriobacteriales
Radhey S. Gupta, Anish Nanda, Bijendra Khadka, Eugene A. Permyakov
PLOS ONE.2017; 12(2): e0172176. CrossRef - Redox Balance in Lactobacillus reuteri DSM20016: Roles of Iron-Dependent Alcohol Dehydrogenases in Glucose/ Glycerol Metabolism
Lu Chen, Paul David Bromberger, Gavin Nieuwenhuiys, Rajni Hatti-Kaul, Shihui Yang
PLOS ONE.2016; 11(12): e0168107. CrossRef
- Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model
-
Ming Zhang , Xing Fan , Bing Fang , Chengzhen Zhu , Jun Zhu , Fazheng Ren
-
J. Microbiol. 2015;53(6):398-405. Published online May 30, 2015
-
DOI: https://doi.org/10.1007/s12275-015-5046-z
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896
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0
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61
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Abstract
-
Probiotics have been suggested as a prophylactic measure
in colon cancer. The aim of this study was to investigate the
impact of Lactobacillus salivarius Ren (Ren) in modulating
colonic microbiota structure and colon cancer incidence in a
rat model after injection with 1,2-dimethyl hydrazine (DMH).
The results indicated that oral administration of Ren could
effectively suppress DMH-induced colonic carcinogenesis.
A significant decrease in cancer incidence (87.5% to 25%)
was detected in rats fed with a dose of 5 × 1010 CFU/kg
bodyweight per day. Using denaturing gradient gel electrophoresis
and Real-time PCR combined with multivariate
statistical methods, we demonstrated that injection with DMH
significantly altered the rat gut microbiota, while Ren counteracted
these DMH-induced adverse effects and promoted
reversion of the gut microbiota close to the healthy state. Tvalue
biplots followed by band sequencing identified 21 bacterial
strains as critical variables affected by DMH and Ren.
Injection of DMH significantly increased the amount of
Ruminococcus species (sp.) and Clostridiales bacteria, as well
as decreasing the Prevotella sp. Administration of Ren reduced
the amount of Ruminococcus sp., Clostridiales bacteria, and
Bacteroides dorei, and increased the amount of Prevotella.
Real-time PCR results were consistent with the results derived
by t-value biplots. These findings suggested that Ren is a potential
agent for colon cancer prevention. In conclusion, the
results
in the present study suggest a potential therapeutic
approach based on the modulation of intestinal microflora
by probiotics may be beneficial in the prevention of colorectal
carcinogenesis.
-
Citations
Citations to this article as recorded by

- Oral administration of Lactobacillus plantarum lysates attenuates the development of atopic dermatitis lesions in mouse models
-
Hangeun Kim , Hye Rim Kim , Na-Ra Kim , Bong Jun Jeong , Jong Suk Lee , Soojin Jang , Dae Kyun Chung
-
J. Microbiol. 2015;53(1):47-52. Published online December 4, 2014
-
DOI: https://doi.org/10.1007/s12275-015-4483-z
-
-
480
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1
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-
29
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Abstract
PDF
-
Lactobacillus plantarum is a well-documented probiotic that
has been used in clinical trials for the regulation of the immune
system and treatment of gastrointestinal diseases. In
this study, we evaluated the effects of L. plantarum cell lysates
on the immune regulation through the in vitro and in vivo
studies. L. plantarum lysates were prepared by sonication
method
, and we observed that the repetition of disruption
step increased indicator components within the bacterial
lysates. Indicator components might affect TNF-α production.
L. plantarum lysates did not induce TNF-α production,
while LPS-induced TNF-α production was dramatically inhibited
in a sonication-dependent manner in THP-1 cells.
Oral administration of L. plantarum lysates effectively attenuated
the horny layer formation and decreased epidermal
thickening in NC/Nga mice skin. The damage to barrier function
after the 8 weeks oral administration was reduced by L.
plantarum lysates as compared to that in the atopic dermatitis
(AD) mice. Further study revealed that L. plantarum lysates
polarized Th1 response via induction of IL-12 and IFN-γ
production and inhibition of IL-4 and IgE production in
NC/Nga mice. Together, our results suggest that L. plantarum
lysates are remarkable material for host homeostasis and it
could be used for the treatment of inflammatory diseases.
-
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Lactobacillus plantarum-
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Review
- MINIREVIEW] The Potential Hazards of Aspergillus sp. in Foods and Feeds, and the Role of Biological Treatment: A Review
-
Sheikh Imranudin Sheikh-Ali , Akil Ahmad , Siti-Hamidah Mohd-Setapar , Zainul Akmal Zakaria , Norfahana Abdul-Talib , Aidee Kamal Khamis , Md Enamul Hoque
-
J. Microbiol. 2014;52(10):807-818. Published online October 1, 2014
-
DOI: https://doi.org/10.1007/s12275-014-4294-7
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385
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0
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16
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Abstract
PDF
-
The contamination of food and feed by Aspergillus has become
a global issue with a significant worldwide economic
impact. The growth of Aspergillus is unfavourable to the
development of food and feed industries, where the problems
happen mostly due to the presence of mycotoxins, which is a
toxic metabolite secreted by most Aspergillus groups. Moreover,
fungi can produce spores that cause diseases, such as
allergies and asthma, especially to human beings. High temperature,
high moisture, retarded crops, and poor food storage
conditions encourage the growth of mold, as well as
the development of mycotoxins. A variety of chemical, biological,
and physical strategies have been developed to control
the production of mycotoxins. A biological approach,
using a mixed culture comprised of Saccharomyces cerevisiae
and Lactobacillus rhamnosus resulted in the inhibition of the
growth of fungi when inoculated into fermented food. The
results
reveal that the mixed culture has a higher potential
(37.08%) to inhibit the growth of Aspergillus flavus (producer
of Aflatoxin) compared to either single culture, L. rhamnosus
NRRL B-442 and S. cerevisiae, which inhibit the growth by
63.07% and 64.24%, respectively.
-
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- Cyclic Dipeptides from Lactic Acid Bacteria Inhibit the Proliferation of Pathogenic Fungi
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Min-Kyu Kwak , Rui Liu , Min-Kyu Kim , Dohyun Moon , Andrew HyoungJin Kim , Sung-Hyun Song , Sa-Ouk Kang
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J. Microbiol. 2014;52(1):64-70. Published online January 4, 2014
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DOI: https://doi.org/10.1007/s12275-014-3520-7
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470
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Abstract
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Lactobacillus plantarum LBP-K10 was identified to be the most potent antifungal strain from Korean traditional fermented vegetables. The culture filtrate of this strain showed remarkable antifungal activity against Ganoderma boninense. Five fractions from the culture filtrate were observed to have an inhibitory effect against G. boninense. Also, the electron ionization and chemical ionization indicated that these compounds might be cyclic dipeptides. Of the five active fractions, two fractions showed the most significant anti-Ganoderma activity, and one of these fractions inhibited the growth of Candida albicans. These compounds were identified to be cis-cyclo(L-Val-L-Pro) and cis-cyclo(L-Phe-L-Pro), as confirmed by X-ray crystallography.
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Min-Kyu Kwak , Rui Liu , Jun-Oh Kwon , Min-Kyu Kim , Andrew HyoungJin Kim , Sa-Ouk Kang
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J. Microbiol. 2013;51(6):836-843. Published online December 19, 2013
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DOI: https://doi.org/10.1007/s12275-013-3521-y
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587
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Abstract
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We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp.,
and Lactococcus lactis.
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Cyclo-(
l
-Phe-
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In Hwang Kim, So-Yeon Kim, Na-Young Park, Yancheng Wen, Keun-Woo Lee, So-Young Yoon, Haneul Jie, Kyu-Ho Lee, Kun-Soo Kim, Shelley M. Payne
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Liming Wu, Huijun Wu, Lina Chen, Hongyue Zhang, Xuewen Gao
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Joo Yeon Kang, Do Kyung Lee, Nam Joo Ha, Hea Soon Shin
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- Lactobacillus paracasei subsp. paracasei LC01 Positively Modulates Intestinal Microflora in Healthy Young Adults
-
Hao Zhang , Jing Sun , Xianting Liu , Chuan Hong , Yuanbo Zhu , Aiping Liu , Siqi Li , Huiyuan Guo , Fazheng Ren
-
J. Microbiol. 2013;51(6):777-782. Published online December 19, 2013
-
DOI: https://doi.org/10.1007/s12275-013-3279-2
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332
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21
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Abstract
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Lactobacillus paracasei subsp. paracasei LC01 (LC01) can
tolerate intestinal stresses and has antioxidant activity. To
evaluate the effect of the bacterium on human intestinal microflora,
a randomized, double-blind, placebo-controlled
human trial was carried out. Fifty-two healthy adult volunteers
were randomized equally to two groups. One group
consumed 12% (wt/vol) skimmed milk supplemented with
1010 CFU of LC01 each day for the 4-week treatment period,
and then consumed placebo in the next treatment period,
separated by a 2-week washout. The other group followed
the reverse order. Group-specific real-time PCR and biochemical
analyses was used to determine the intestinal bacterial
composition of fecal samples collected at the end of
every period, and the concentration of short-chain fatty acids
and ammonia. A significant inhibition in fecal Escherichia
coli and increase in Lactobacillus, Bifidobacterium, and Roseburia
intestinalis were observed after consumption of LC01.
Acetic acid and butyric acid were significantly higher in the
probiotic stage and fecal ammonia was significantly lower.
The results indicated a modulation effect of LC01 on the
intestinal microflora of young adults, suggesting a beneficial
effect on bowel health. LC01 may have potential value as a
probiotic.
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Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1
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Spp.: A Marker of Health?
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Esther Nova, Fátima Pérez de Heredia, Sonia Gómez‐Martínez, Ascensión Marcos
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Gregor Reid
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Muriel Derrien, Johan E.T. van Hylckama Vlieg
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Joo Yeon Kang, Do Kyung Lee, Nam Joo Ha, Hea Soon Shin
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- Lactobacillus salivarius Strain FDB89 Induced Longevity in Caenorhabditis elegans by Dietary Restriction
-
Yang Zhao , Liang Zhao , Xiaonan Zheng , Tianjiao Fu , Huiyuan Guo , Fazheng Ren
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J. Microbiol. 2013;51(2):183-188. Published online April 27, 2013
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DOI: https://doi.org/10.1007/s12275-013-2076-2
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234
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46
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Abstract
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In this study, we utilized the nematode Caenorhabditis elegans to assess potential life-expanding effect of Lactobacillus salivarius strain FDB89 (FDB89) isolated from feces of centenarians in Bama County (Guangxi, China). This study showed that feeding FDB89 extended the mean life span in C. elegans by up to 11.9% compared to that of control nematodes. The reduced reproductive capacities, pharyngeal pumping rate, growth, and increased superoxide dismutase (SOD) activity and XTT reduction capacity were also observed in FDB89 feeding worms. To probe the anti-aging mechanism further, we incorporated a food gradient feeding assay and assayed the life span of eat-2 mutant. The results demonstrated that the maximal life span of C. elegans fed on FDB89 was achieved at the concentration of 1.0 mg bacterial cells/plate, which was 10-fold greater than that of C. elegans fed on E. coli OP50 (0.1 mg bacterial cells/plate). However, feeding FDB89 could not further extend the life span of eat-2 mutant. These results indicated that FDB89 modulated the longevity of C. elegans in a dietary restriction-dependent manner and expanded the understanding of anti-aging effect of probiotics.
- Lactobacillus delbrueckii ssp. lactis R4 Prevents Salmonella typhimurium SL1344-Induced Damage to Tight Junctions and Adherens Junctions
-
Qinghua Yu , Liqi Zhu , Zhisheng Wang , Pengcheng Li , Qian Yang
-
J. Microbiol. 2012;50(4):613-617. Published online August 25, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1596-5
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291
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0
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12
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Abstract
PDF
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Cell junctions are the gatekeepers of the paracellular route and defend the mucosal barrier. Several enteropathogenic bacteria can invade intestinal epithelial cells by targeting and damaging cell junctions. It is not well understood how Salmonella typhimurium is able to overcome the intestinal barrier and gain access to the circulation, nor is it understood how Lactobacillus prevents the invasion of S. typhimurium. Therefore, we sought to determine whether infection with S. typhimurium SL1344 could regulate the molecular composition of cell junctions and whether Lactobacillus delbrueckii ssp. lactis R4 could affect this modification. Our data demonstrated that infection of Caco-2 cells with S. typhimurium over 2 h resulted in a redistribution of claudin-1, ZO-1, occluding, and E-cadherin. Western blot analysis of epithelial cell lysates demonstrated that S. typhimurium could decrease the expression of cell junction proteins. However, L. delbrueckii ssp. lactis R4 ameliorated this destruction and induced increased expression of ZO-1, occludin, and E-cadherin relative to the levels in the control group. The results of these experiments implied that S. typhimurium may facilitate its uptake and distribution within the host by regulating the molecular composition of cell junctions. Furthermore, Lactobacillus may prevent the adhesion and invasion of pathogenic bacteria by maintaining cell junctions and the mucosal barrier.
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- Postbiotic Lipoteichoic acid of probiotic Lactobacillus origin ameliorates inflammation in HT-29 cells and colitis mice
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Nafiseh Davati, Shohreh Hesami
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Na Young Lee, Hyun Chae Joung, Byoung Kook Kim, Byung Yong Kim, Tae Sik Park, Ki Tae Suk
Gut Microbes.2020; 12(1): 1829449. CrossRef - Effects of multi-strain probiotic supplementation on intestinal microbiota, tight junctions, and inflammation in young broiler chickens challenged with Salmonella enterica subsp. enterica
Chi Huan Chang, Po Yun Teng, Tzu Tai Lee, Bi Yu
Asian-Australasian Journal of Animal Sciences.2020; 33(11): 1797. CrossRef - Sodium houttuyfonate enhances the intestinal barrier and attenuates inflammation induced by Salmonella typhimurium through the NF-κB pathway in mice
Lei Zhang, Hao Lv, Ying Li, Na Dong, Chongpeng Bi, Anshan Shan, Zhihan Wu, Baoming Shi
International Immunopharmacology.2020; 89: 107058. CrossRef - Cyperus esculentus L. Tubers (Tiger Nuts) Protect Epithelial Barrier Function in Caco-2 Cells Infected by Salmonella Enteritidis and Promote Lactobacillus plantarum Growth
David Moral-Anter, Joan Campo-Sabariz, Ruth Ferrer, Raquel Martín-Venegas
Nutrients.2020; 13(1): 71. CrossRef - Evaluation of in ovo Bacillus spp. based probiotic administration on horizontal transmission of virulent Escherichia coli in neonatal broiler chickens
M.A. Arreguin-Nava, B.D. Graham, B. Adhikari, M. Agnello, C.M. Selby, X. Hernandez-Velasco, C.N. Vuong, B. Solis-Cruz, D. Hernandez-Patlan, J.D. Latorre, G. Tellez, B.M. Hargis, G. Tellez-Isaias
Poultry Science.2019; 98(12): 6483. CrossRef - Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults
Margarita A. Arreguin-Nava, Daniel Hernández-Patlán, Bruno Solis-Cruz, Juan D. Latorre, Xochitl Hernandez-Velasco, Guillermo Tellez, Saeed El-Ashram, Billy M. Hargis, Guillermo Tellez-Isaias
Animals.2019; 9(9): 696. CrossRef - Ethyl pyruvate protects against Salmonella intestinal infection in mice through down-regulation of pro-inflammatory factors and inhibition of TLR4/MAPK pathway
Na Dong, Xinyao Xu, Chenyu Xue, Chensi Wang, Xinran Li, Anshan Shan, Li Xu, Deshan Li
International Immunopharmacology.2019; 71: 155. CrossRef - In ovo evaluation of FloraMax®-B11 on Marek’s disease HVT vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and Salmonella enteritidis infection in broiler chickens
K.D. Teague, L.E. Graham, J.R. Dunn, H.H. Cheng, N. Anthony, J.D. Latorre, A. Menconi, R.E. Wolfenden, A.D. Wolfenden, B.D. Mahaffey, M. Baxter, X. Hernandez-Velasco, R. Merino-Guzman, L.R. Bielke, B.M. Hargis, G. Tellez
Poultry Science.2017; 96(7): 2074. CrossRef - Salmosan, a β-Galactomannan-Rich Product, Protects Epithelial Barrier Function in Caco-2 Cells Infected by Salmonella enterica Serovar Enteritidis
M Teresa Brufau, Joan Campo-Sabariz, Ricard Bou, Sergi Carné, Joaquim Brufau, Borja Vilà, Ana M Marqués, Francesc Guardiola, Ruth Ferrer, Raquel Martín-Venegas
The Journal of Nutrition.2016; 146(8): 1492. CrossRef
- Effect of Lactobacillus reuteri on the Proliferation of Propionibacterium acnes and Staphylococcus epidermidis
-
Mi-Sun Kang , Jong-Suk Oh , Seok-Woo Lee , Hoi-Soon Lim , Nam-Ki Choi , Seon-Mi Kim
-
J. Microbiol. 2012;50(1):137-142. Published online February 27, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1286-3
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1,076
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1
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14
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Abstract
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While it is generally accepted that Propionibacterium acnes
is involved in the development of acne, other bacteria including
Staphylococcus epidermidis have also been isolated
from the acne lesion. The interaction between Lactobacillus
reuteri, a probiotic bacterium, and acnegenic bacteria is
unclear. This study examined the effects of L. reuteri on the
proliferation of P. acnes and S. epidermidis. Human-derived
L. reuteri strains (KCTC 3594 and KCTC 3678) and rat-derived
L. reuteri KCTC 3679 were used. All strains exhibited
significant inhibitory effects on the growth of P. acnes and
S. epidermidis. The proliferation of P. acnes was decreased
by 2-log scales after incubation with L. reuteri for 24 h. In
addition, the proliferation of S. epidermidis was decreased
by 3-log scales after incubation with L. reuteri for 24 h,
whereas the growth of L. reuteri was unaffected by P. acnes
or S. epidermidis. Among the L. reuteri strains examined, L.
reuteri KCTC 3679 had the strongest inhibitory effect on
the growth of P. acnes and S. epidermidis, followed by L.
reuteri KCTC 3594 and L. reuteri KCTC 3678. Interestingly,
reuterin, an antimicrobial factor, was produced only by L.
reuteri KCTC 3594. The most pronounced the antibacterial
activities of L. reuteri were attributed to the production of
organic acids. Overall, these results suggest that L. reuteri
may be a useful probiotic agent to control the growth of
bacteria involved in acne inflammation and prevent acne.
-
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Ming Zhang , Xuewei Qiao , Liang Zhao , Lu Jiang , Fazheng Ren
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DOI: https://doi.org/10.1007/s12275-011-1137-7
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255
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Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1- Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. salivarius REN significantly altered the bacterial communities of rat colons. A total of 27 bacterial strains were identified as being affected by treatment with 4-Nitroquinoline-1-Oxide or L. salivarius REN using a t-value biplot combined with band sequencing. 4-Nitroquinoline-1-Oxide treatment increased the abundance of two potential pathogens (one Helicobacter strain and one Desulfovibrio strain), as well as reducing the abundance of two potentially beneficial strains (one Ruminococcaceae strain and one Rumen bacteria). The Helicobacter strain was initally detected in carcinogen-treated rat intestinal microflora, but L. salivarius REN treatment effectively suppressed the growth of the Helicobacter strain. These results suggested that L. salivarius REN may be a potential probiotic, efficiently acting against the initial infection with, and the growth of pathogenic bacteria.
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- Understanding Ligilactobacillus salivarius from Probiotic Properties to Omics Technology: A Review
Yong Yang, Xin Song, Guangqiang Wang, Yongjun Xia, Zhiqiang Xiong, Lianzhong Ai
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- Expression of the Lactobacillus plantarum malE Gene Is Regulated by CcpA and a MalR-Like Protein
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Lidia Muscariello , Valeria Vastano , Rosa A. Siciliano , Margherita Sacco , Rosangela Marasco
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J. Microbiol. 2011;49(6):950-955. Published online December 28, 2011
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DOI: https://doi.org/10.1007/s12275-011-0495-5
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Lactobacillus plantarum is commonly used in the food industry as a starter in various fermentations, especially in vegetable fermentations, in which starch is a common substrate. This polysaccharide, which is obtained from potatoes or corn and is hydrolysed mainly to maltose and glucose by acids or enzymes, is commercially used for the production of lactate by lactic acid fermentation. In this study, we describe the regulation of malE gene expression in L. plantarum. This gene, located in a 7-gene cluster, probably organized in an operon, encodes a putative maltose/maltodextrin-binding protein. We studied the expression of malE in L. plantarum LM3 (wild type) and in LM3-2 (ccpA1), which carries a null mutation in the ccpA gene, encoding the catabolite control protein A (CcpA). In the presence of glucose, expression of the MalE protein was higher in the mutant strain as compared to that in the wild-type strain. Transcription of the malE gene was induced by maltose and regulated by a CcpA-mediated carbon catabolite repression. Further, we isolated strains carrying mutations in 2 genes, lp_0172 and lp_0173, whose deduced amino acid sequences share significant identity with MalR, a regulator of the maltose operon in several gram-positive bacteria. A double mutant exhibited glucose-insensitive malE transcription, while absence of the functional Lp_0172 open reading frame had no effect on malE expression.
- Inhibitory Effect of Lactobacillus reuteri on Periodontopathic and Cariogenic Bacteria
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Mi-Sun Kang , Jong-Suk Oh , Hyun-Chul Lee , Hoi-Soon Lim , Seok-Woo Lee , Kyu-Ho Yang , Nam-Ki Choi , Seon-Mi Kim
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J. Microbiol. 2011;49(2):193-199. Published online May 3, 2011
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DOI: https://doi.org/10.1007/s12275-011-0252-9
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The interaction between Lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. This study examined the effects of L. reuteri on the proliferation of periodontopathic bacteria including Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Tannerella forsythia, and on the formation of Streptococcus mutans biofilms. Human-derived L. reuteri strains (KCTC 3594 and KCTC 3678) and rat-derived L. reuteri KCTC 3679 were used. All strains exhibited significant inhibitory effects on the growth of periodontopathic bacteria and the formation of S. mutans
biofilms. These antibacterial activities of L. reuteri were attributed to the production of organic acids, hydrogen peroxide, and a bacteriocin-like compound. Reuterin, an antimicrobial factor, was produced only by L. reuteri KCTC 3594. In addition, L. reuteri inhibited the production of methyl mercaptan by F. nucleatum and P. gingivalis. Overall, these results suggest that L. reuteri may be useful as a probiotic agent for improving oral health.
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Deog Yong Lee , Yeon-Soo Seo , Nabin Rayamajhi , Mi Lan Kang , Su In Lee , Han Sang Yoo
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J. Microbiol. 2009;47(6):663-672. Published online February 4, 2010
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DOI: https://doi.org/10.1007/s12275-009-0124-8
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Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2~10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.
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- NOTE] Microbial Population Dynamics and Temperature Changes during Fermentation of Kimjang Kimchi
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Dongyun Lee , Sunjoo Kim , Jinhee Cho , Jeongho Kim
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J. Microbiol. 2008;46(5):590-593. Published online October 31, 2008
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DOI: https://doi.org/10.1007/s12275-008-0156-5
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A distinct subset of lactic acid bacteria that are greatly influenced by temperature play an important role during kimchi fermentation. However, microbial population dynamics and temperature control during kimjang kimchi fermentation, which is traditionally fermented underground, are not known. Here we show that Lactobacillus sakei predominates in kimjang kimchi, perhaps due to suitable fermentation (5~9°C) and storage (-2°C) temperatures. The temperature of this kimchi gradually decreased to 3.2°C during the first 20 days of fermentation (-0.3°C/day) and then was stably maintained around 1.6°C, indicating that this simple approach is very efficient both for fermentation and storage. These findings provide important information towards the development of temperature controlling systems for kimchi fermentation.
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International Journal of Refrigeration.2025; 180: 116. CrossRef - Sonmat, a citizen-science enabled Kimjang kimchi case study on associations between hand and kimchi microbiota
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Xinyuan Zhang, Qiong Wang, Yuanhong Xie, Hongxing Zhang, Junhua Jin, Yong Xiong, Xiaona Pang, Frank Vriesekoop
Fermentation.2024; 10(7): 333. CrossRef - Influence of Isolation Temperature on Isolating Diverse Lactic Acid Bacteria from Kimchi and Cultural Characteristics of Psychrotrophs
Hye In Ko, Chang Hee Jeong, Se-Jin Park, So-Rim Kim, Jong-Bang Eun, Tae-Woon Kim
Journal of Microbiology and Biotechnology.2023; 33(8): 1066. CrossRef - 비건 김치 발효 중 대사산물과 미생물 군집의 변화
미희 박, 윤정 최, 주영 임, 성희 박, 승기 민, 미애 이
Korean Journal of Food and Cookery Science.2023; 39(3): 168. CrossRef - Transcriptional analysis of the molecular mechanism underlying the response of Lactiplantibacillus plantarum to lactic acid stress conditions
Ha-Young Jang, Min Ji Kim, Minseo Bae, In Min Hwang, Jong-Hee Lee
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International Food Research Journal.2021; 28(6): 1285. CrossRef - Initial storage of kimchi at room temperature alters its microbial and metabolite profiles
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Seong-Eun Park, Seung-Ho Seo, Eun-Ju Kim, Chang-Su Na, Hong-Seok Son
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Zhichang Qiu, Ningyang Li, Xiaoming Lu, Zhenjia Zheng, Mingjie Zhang, Xuguang Qiao
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Frontiers in Microbiology.2017;[Epub] CrossRef - Antibacterial activity of supernatant obtained from Weissella koreensis and Lactobacillus sakei on the growth of pathogenic bacteria
Hana Im, Joon-Kwan Moon, Woan-Sub Kim
Korean Journal of Agricultural Science.2016; 43(3): 415. CrossRef - Kimchi and Other Widely Consumed Traditional Fermented Foods of Korea: A Review
Jayanta Kumar Patra, Gitishree Das, Spiros Paramithiotis, Han-Seung Shin
Frontiers in Microbiology.2016;[Epub] CrossRef - Source Tracking and Succession of Kimchi Lactic Acid Bacteria during Fermentation
Se Hee Lee, Ji Young Jung, Che Ok Jeon
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Ruo‐si Fang, Ya‐chen Dong, Feng Chen, Qi‐he Chen
Journal of Food Science.2015;[Epub] CrossRef - Effect of Kimchi Fermentation on Oxalate Levels in Silver Beet (Beta vulgaris var. cicla)
Yukiko Wadamori, Leo Vanhanen, Geoffrey Savage
Foods.2014; 3(2): 269. CrossRef - Metagenomic analysis for identifying Kimchi sp. during the industrial-scale batch fermentation
Ji-Young Ahn, Jiho Min, Sang-Hee Lee, Am Jang, Choi-Kyu Park, Soon-Dong Kwon, Sang-Kyu Park, Kibeom Lee, Yang-Hoon Kim
Toxicology and Environmental Health Sciences.2014; 6(1): 8. CrossRef - Health Benefits of Kimchi (Korean Fermented Vegetables) as a Probiotic Food
Kun-Young Park, Ji-Kang Jeong, Young-Eun Lee, James W. Daily
Journal of Medicinal Food.2014; 17(1): 6. CrossRef - Effects of red pepper powder on microbial communities and metabolites during kimchi fermentation
Sang Hyeon Jeong, Hyo Jung Lee, Ji Young Jung, Se Hee Lee, Hye-Young Seo, Wan-Soo Park, Che Ok Jeon
International Journal of Food Microbiology.2013; 160(3): 252. CrossRef - Microbial Succession and Metabolite Changes during Long‐Term Storage of Kimchi
Sang Hyeon Jeong, Se Hee Lee, Ji Young Jung, Eun Jin Choi, Che Ok Jeon
Journal of Food Science.2013;[Epub] CrossRef - Effects of Leuconostoc mesenteroides starter cultures on microbial communities and metabolites during kimchi fermentation
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International Journal of Food Microbiology.2012; 153(3): 378. CrossRef - Novel Podoviridae Family Bacteriophage Infecting Weissella cibaria Isolated from Kimchi
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Applied and Environmental Microbiology.2011; 77(7): 2264. CrossRef - Effect of Lactobacillus pentosus-Fermented Artemisiae Argi Folium on Nitric Oxide Production of Macrophage impaired with Various Toxicants
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Journal Articles
- Improved Production of Live Cells of Lactobacillus rhamnosus by Continuous Cultivation using Glucose-yeast Extract Medium
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Liew Siew Ling , Rosfarizan Mohamad , Raha Abdul Rahim , Ho Yin Wan , Arbakariya Bin Ariff
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J. Microbiol. 2006;44(4):439-446.
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DOI: https://doi.org/2408 [pii]
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Abstract
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In this study, the growth kinetics of Lactobacillus rhamnosus and lactic acid production in continuous culture were assessed at a range of dilution rates (0.05 h?1 to 0.40 h?1) using a 2 L stirred tank fermenter with a working volume of 600 ml. Unstructured models, predicated on the Monod and Luedeking-Piret equations, were employed to simulate the growth of the bacterium, glucose consumption, and lactic acid production at different dilution rates in continuous cultures. The maximum specific growth rate of L. rhamnosus, ┢max, was estimated at 0.40 h?1, and the Monod cell growth saturation constant, Ks, at approximately 0.25 g/L. Maximum cell viability (1.3 ≠ 1010 CFU/ml) was achieved in the dilution rate range of D = 0.28 h?1 to 0.35 h?1. Both maximum viable cell yield and productivity were achieved at D = 0.35 h?1. The continuous cultivation of L. rhamnosus at D = 0.35 h?1 resulted in substantial improvements in cell productivity, of 267% (viable cell count) that achieved via batch cultivation.
- Plasmid Profiling and Curing of Lactobacillus Strains Isolated from the Gastrointestinal Tract of Chicken
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Sieo Chin Chin , Norhani Abdullah , Tan Wen Siang , Ho Yin Wan
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J. Microbiol. 2005;43(3):251-256.
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DOI: https://doi.org/2217 [pii]
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In this study, we assessed the susceptibility of 12 Lactobacillus strains, all of which had been isolated from the gastrointestinal tracts of chicken, to three antibiotics (chloramphenicol, erythromycin and tetracycline) used commonly as selective markers in transformation studies of lactic acid bacteria. Among these strains, 17%, 58%, and 25% were found to exhibit a high degree of resistance to 200 mg/ml of tetracycline, erythromycin, and chloramphenicol, respectively. Seven of the 12 Lactobacillus strains exhibiting resistance to at least 50 mg/ml of chloramphenicol or erythromycin, and five strains exhibiting resistance to at least 50 mg/ml of tetracycline, were subsequently subjected to plasmid curing with chemical curing agents, such as novobiocin, acriflavin, SDS, and ethidium bromide. In no cases did the antibiotic resistance of these strains prove to be curable, with the exception of the erythromycin resistance exhibited by five Lactobacillus strains (L. acidophilus I16 and I26, L. fermentum I24 and C17, and L. brevis C10). Analysis of the plasmid profiles of these five cured derivatives revealed that all of the derivatives, except for L. acidophilus I16, possessed profiles similar to those of wild-type strains. The curing of L. acidophilus I16 was accompanied by the loss of 4.4 kb, 6.1 kb, and 11.5 kb plasmids.
- Optimization of Lactic Acid Production in SSF by Lactobacillus amylovorus NRRL B-4542 Using Taguchi Methodology
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Pyde Acharya Nagarjun , Ravella Sreenivas Rao , Swargam Rajesham , Linga Venkateswar Rao
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J. Microbiol. 2005;43(1):38-43.
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DOI: https://doi.org/2140 [pii]
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Lactic acid production parameter optimization using Lactobacillus amylovorus NRRL B-4542 was performed using the design of experiments (DOE) available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, moisture, yeast extract, MgSO_4 . 7H_20, Tween 80, and corn steep liquor (CSL) were determined. Among the physical parameters, temperature contributed higher influence, and among media components, yeast extract, MgSO_4 . 7H_20, and Tween 80 played important roles in the conversion of starch to lactic acid. The expected yield of lactic acid under these optimal conditions was 95.80% and the actual yield at optimum conditions was 93.50%.
Published Erratums
- [Erratum] A split face study on the effect of an anti-acne product containing fermentation products of Enterococcus faecalis CBT SL-5 on skin microbiome modification and acne improvement
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Hye Sung Han , Sun Hye Shin , Bo-Yun Choi , Nayeon Koo , Sanghyun Lim , Dooheon Son , Myung Jun Chung , Kui Young Park , Woo Jun Sul
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J. Microbiol. 2022;60(7):766-766.
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DOI: https://doi.org/10.1007/s12275-022-1682-2
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484
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- Skin Microbiome and Acne: Microbial Imbalances and Impact – Interview with Three Key Opinion Leaders
Brigitte Scott
EMJ Dermatology.2024; : 83. CrossRef
- Erratum] Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments
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Shengxiang Pei , Siwen Niu , Fuquan Xie , Wenjing Wang , Shuang Zhang , Gaiyun Zhang
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J. Microbiol. 2021;59(11):1064-1065.
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DOI: https://doi.org/10.1007/s12275-021-0235-4
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340
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Brevibacterium koreense sp. nov., a moderately halophilic bacterium isolated from jogae-jeotgal, a Korean fermented seafood
Sohee Nam, Yujin Kim, Min Ji Lee, Yeon Bee Kim, Jeong Ui Yun, Mi-Ja Jung, Hye Seon Song, Se Hee Lee, Seok-Jun Kim, Tae Woong Whon
International Journal of Systematic and Evolutionary Microbiology
.2025;[Epub] CrossRef
- Characterization of Isolated Lactobacillus spp. and Classification by RAPD-PCR Analysis
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Oh-Sik Kwon
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J. Microbiol. 2000;38(3):137-144.
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The genetic relationships of six Lactobacillus strains and five laboratory isolates from fermented milk were determined by a random amplified polymorphic DNA (RAPD)-Polymease chan reaction (PCR) method. With 42 random primers, the results were analyzed by using the NTSYS-PC software for phenetic analysis. It revealed that all tested bacteria were divided into three distinct clusters. The clusters implied three subgenuses existed for the genus Lactobacillus, which were previously proposed by Rogosa and Sharpe. From the results, it was also possible to determine that the isolated Lactobacillus strains from fermented milk were grouped into L. acidophilus or L. bulgaricus. Interestingly, the three tested L. casei strains were divided into different clusters implying different subgenuses, i.e., Thermobacterium (L. casei YIT 9018) and Strepto-bacterium (L. casei CHR. Hansen and L. casei ATCC 4646). According to the distance matrix generated by an UPGMA program, the isolated bacteria LT01 and LT02 were determined as a subspecies of L. bulgaricus. The HK01, HK02 and HK03 were very closely related to either L. acidophilus or L. casei YIT 9018. Hence, RAPD-PCR appears to be a very practical method to determine the genetic relationships of the Lactobacillus species and to characterize the unknown Lactobacillus strains at the subspecies level.
- Inhibitory Effects of Lactic Acid Bacteria (LAB) on the Azoxymethane-induced Colonic Preneoplastic Lesions
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Sang-Myeong Lee , Wan-Kyu Lee
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J. Microbiol. 2000;38(3):169-175.
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Abstract
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Epidemiological and experimental studies provide evidences that diet and intestinal microflora play an important role in colon carcinogenesis. In recent years, it has been suggested that lactic acid bacteria (LAB) used to ferment dairy products have an inhibitory effect on the colon cancer. This study was designed to determine the effect of Bifidobacterium longum HY8001 (Bif) and Lactobacillus acidophilus HY2104 (Lac) of Korean origin on azoxymethane (AOM)-induced colonic preneoplastic lesions such as aberrant crypt foci (ACF) formation and cecal pH. At five weeks of age, Spraque-Dawley rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Animals were weighed weekly and oral administration of LAB cultures were performed daily until the termination of the study. Two weeks later, all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once per week for 2 weeks. All rats were necropsied 7 weeks after the last AOM injection, and the ACF were visualized under light microscopy in the formalin-fixed, unsectioned methylene blue-stained colons. The total number of aberrant crypt in Bif, Lac, and Bif+Lac groups were significantly lower than that of the AOM alone group and the percentage of inhibitions weas 35.0, 45.4 and 45.0%, respectively. Significant inhibition (p<0.001) in the total number of ACF was also observed in LAB treated groups (Bif, Lac, and Bif+Lac group by 30.3, 38.6, and 41.2%, respec-tively). Furthermore, cecal pH appeared to significantly decrease by LAB administration. The results of present study provide some evidences for potential colon tumor-inhibitory properties of lactic cultures and fermented dairy products.
- Antibacterial Activities of Lactobacillus crispatus ATCC 33820 and Lactobacillus gasseri ATCC 33323
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Jin-Woo Kim , S.N. Rajagopal
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J. Microbiol. 2001;39(2):146-148.
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Lactobacillus crispatus ATCC 33820 and L. gasseri ATCC 33323 were grown in MRS broth (pH 6.5) at 37 C for 24 h and the antibacterial activities of cell free culture supernatants were determined by the agar well diffusion method. The culture supernatants were inhibitory to Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Pediococcus acidilacticii, and Lactobacillus helveticus. The supernatants did not show any lysozyme activity. Addition of catalase did not affect the antibacterial activities of the supernatants. The antibacterial substances were heat stable (100 C for 60 min) and sensitive to proteases.
- Effects of Lactic Acid Bacteria on Intestinal Microbial Enzyme Activity and Composition in Rats Treated with Azoxymethane
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Sang-Myeong Lee , Wan-Kyu Lee
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J. Microbiol. 2001;39(3):154-161.
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Abstract
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In recent years, colon cancer has been reported to be one of the most important causes of cancer morbidity and mortality in Korea. Epidemiological and experimental studies suggest that lactic acid bacteria (LAB) used to ferment dairy products inhibits colon carcinogenesis. The present study was designed to determine whether the colon cancer inhibitory effect of LAB (Bifidobacterium longum HY8001; Bif and Lactobacillus acidophilus HY2104; Lac) of Korean origin, is associated with intestinal microflora composition and certain enzyme activity in rats treated with azoxymethane (AOM). At five weeks of age, SD rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Oral administration of lactic acid bacteria cultures were performed daily until the termination of the study. Two weeks later, all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once weekly for 2 weeks. Every two weeks for 10 weeks, five of the rats in each group were randomly chosen for fecal specimen collection. The fecal specimens were used for assay of [beta]-glucuronidase and nitroreductase, and analysis of intestinal microflora composition. The activity of [beta]-glucuronidase which plays an important role in the production of the carcinogenic metabolite of azoxymethane was remarkably increased in the AOM alone group after AOM injection and maintained the high level during the experiment. However, LAB inhibited the AOM-induced increase in [beta]-glucuronidase activity. Nitroreductase activity decreased by 30-40% in LAB treated groups in comparison with that of the AOM alone group. The results of the present study suggest that LAB inhibits colon carcinogenesis by modulating the metabolic activity of intestinal microflora and improving the composition of intestinal microflora.