Journal of Microbiology

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Genetic variation of Colletotrichum magnum isolated from Carica papaya as revealed by DNA fingerprinting: Daisy Pérez-Brito , Alberto Cortes-Velázquez , Teresita Valencia-Yah , Anuar Magaña-Álvarez , Cuauhtémoc Navarro , Blanca Moreno , Steven Quiroga , Raúl Tapia-Tussell; J. Microbiol. 2018;56(11):813-821. Published online October 24, 2018; DOI: https://doi.org/10.1007/s12275-018-8215-z

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Mexico is one of the five largest producers of papaya worldwide, but losses caused by pathogens, mainly fungus, at the pre- and post-harvest stages are often more than 50% of the crop. Papaya anthracnose, caused by three different species of the Colletotrichum genus in Mexico, occupies a preponderant place in this problem. Although two of these species, C. gloeosporiodes and C. truncatum, have been characterized morphologically and genotypically, this has not occurred with C. magnum, the third species involved, about which there is very little information. Because of this, it is vital to know its genetic characterization, much more so considering that the studies carried out on the other two species reveal a wide genetic diversity, differences in pathogenicity and in the response to fungicides of the different strains characterized. In this work, Colletotrichum spp. isolates were collected at different papaya orchards in the south-southeast of Mexico. C. magnum isolates identified by species-specific primers were characterized by morphological and molecular approaches. Differences in colony characteristics resulted in five morphological groups. AP-PCR, DAMD and ISSR markers were found to be very efficient for revealing the interspecific variability of this species. The high genetic variability found in the accessions of C. magnum was linked to the geographical area where they were collected. Isolates from Chiapas State were the most variable, showing point mutations in the ITS1- ITS2 region. These results will enable a better phytosanitary management of anthracnose in papaya in this region of Mexico.

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Current technologies to control fungal diseases in postharvest papaya (Carica papaya L.)
Juliana Pereira Rodrigues, Caroline Corrêa de Souza Coelho, Antonio Gomes Soares, Otniel Freitas-Silva
Biocatalysis and Agricultural Biotechnology.2021; 36: 102128. CrossRef

Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting: Daisy Pérez-Brito , Anuar Magaña-Alvarez , Patricia Lappe-Oliveras , Alberto Cortes-Velazquez , Claudia Torres-Calzada , Teófilo Herrera-Suarez , Alfonso Larqué-Saavedra , Raul Tapia-Tussell; J. Microbiol. 2015;53(1):14-20. Published online January 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4373-4

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Abstract

This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing polymorphisms between isolates of this species.

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Clavispora lusitaniae: From a saprophytic yeast to an emergent pathogen
Olga C. Rojas, Alexandra M. Montoya, Rogelio de J. Treviño-Rangel
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Effect of precursors and stress factors on yeast isolated from fermented maesil extract and their biogenic amine formation
So Hee Yoon, Sanghyeon Lee, Sun-Young Lee, BoKyung Moon
Food Science and Biotechnology.2024; 33(1): 211. CrossRef
Isolation of Clavispora lusitaniae from the Oral Cavity of Immunocompetent Young Adults from the North of Mexico
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Indian Journal of Microbiology.2024; 64(2): 475. CrossRef
Influence of the Biotechnological Process of Mezcal Fermentation on Yeast Diversity in Four palenques of Oaxaca, Mexico
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Yeast Microbiota during Sauerkraut Fermentation and Its Characteristics
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Potential production of 2-phenylethanol and 2-phenylethylacetate by non-Saccharomyces yeasts from Agave durangensis
Pablo Jaciel Adame-Soto, Elva Teresa Aréchiga-Carvajal, Mercedes G López, Silvia Marina González-Herrera, Martha Rocio Moreno-Jiménez, Norma Urtiz-Estrada, Olga Miriam Rutiaga-Quiñones
Annals of Microbiology.2019; 69(9): 989. CrossRef
Genetic variation of Colletotrichum magnum isolated from Carica papaya as revealed by DNA fingerprinting
Daisy Pérez-Brito, Alberto Cortes-Velázquez, Teresita Valencia-Yah, Anuar Magaña-Álvarez, Cuauhtémoc Navarro, Blanca Moreno, Steven Quiroga, Raúl Tapia-Tussell
Journal of Microbiology.2018; 56(11): 813. CrossRef

Molecular Characterization of Vibrio cholerae Isolates from Cholera Outbreaks in North India: Joseph J. Kingston , Kuruvilla Zachariah , Urmil Tuteja , Sanjay Kumar , Harsh Vardhan Batra; J. Microbiol. 2009;47(1):110-115. Published online February 20, 2009; DOI: https://doi.org/10.1007/s12275-008-0162-7

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Abstract: Vibrio cholerae isolates recovered from cholera outbreaks in Bhind district of Madhya Pradesh and Delhi, Northern India were characterized. The O1 serogroup isolates from Bhind outbreak were of Inaba serotype whereas both Ogawa and Inaba serotypes were recovered from Delhi. PCR analysis revealed that only O1 serogroup V. cholerae isolates carried the virulence-associated genes like ctxA, tcpA, ace, and zot. Molecular typing by repetitive sequence based ERIC, VCR1, and VC1 PCR’s revealed similar DNA profile for both Inaba and Ogawa serotypes. A discrete VC1-PCR band identified among the El Tor strains had greater similarity (>97%) to the V. cholerae genome sequence and therefore has the potential to be used as a marker for the identification of the V. cholerae strains. Non-O1 strains recovered from Bhind region differed among themselves as well as from that of the O1 isolates. All the O1 serogroup isolates possessed SXT element and were uniformly resistant to the antibiotics nalidixic acid, polymyxin-B, furazolidone, cloxacilin, trimethoprim-sulfamethaxazole, and vibriostatic agent O129. Inaba strains from both Delhi and Bhind differed from Ogawa strains by their resistance to streptomycin despite sharing similar DNA patterns in all the three rep-PCRs. Though Delhi and Bhind are separate geographical regions in Northern India, Inaba strains from both these places appear to be closely related owing to their similarity in antibiogram and genetic profile.

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