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- Impact of small RNA RaoN on nitrosative-oxidative stress resistance and virulence of Salmonella enterica serovar Typhimurium
- Sinyeon Kim , Yong Heon Lee
- J. Microbiol. 2020;58(6):499-506. Published online April 11, 2020
- DOI: https://doi.org/10.1007/s12275-020-0027-2
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- 5 Web of Science
- 6 Crossref
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Abstract
-
RaoN is a Salmonella-specific small RNA that is encoded in
the cspH-envE intergenic region on Salmonella pathogenicity
island-11. We previously reported that RaoN is induced under
conditions of acid and oxidative stress combined with nutrient
limitation, contributing to the intramacrophage growth
of Salmonella enterica serovar Typhimurium. However, the
role of RaoN in nitrosative stress response and virulence has
not yet been elucidated. Here we show that the raoN mutant
strain has increased susceptibility to nitrosative stress by
using a nitric oxide generating acidified nitrite. Extending
previous research on the role of RaoN in oxidative stress resistance,
we found that NADPH oxidase inhibition restores
the growth of the raoN mutant in LPS-treated J774A.1 macrophages.
Flow cytometry analysis further revealed that the
inactivation of raoN leads to an increase in the intracellular
level of reactive oxygen species (ROS) in Salmonella-infected
macrophages, suggesting that RaoN is involved in the inhibition
of NADPH oxidase-mediated ROS production by mechanisms
not yet resolved. Moreover, we evaluated the effect
of raoN mutation on the virulence in murine systemic
infection and determined that the raoN mutant is less virulent
than the wild-type strain following oral inoculation. In
conclusion
, small regulatory RNA RaoN controls nitrosativeoxidative stress resistance and is required for virulence of Salmonella in mice. -
Citations
Citations to this article as recorded by
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