- Development of a Novel Korean H9‑Specific rRT‑PCR Assay and Its Application for Avian Influenza Virus Surveillance in Korea
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Mingeun Sagong , Yong-Myung Kang , Na Yeong Kim , Eun Bi Noh , Gyeong-Beom Heo , Se-Hee An , Youn-Jeong Lee , Young Ki Choi , Kwang-Nyeong Lee
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J. Microbiol. 2023;61(10):929-936. Published online November 27, 2023
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DOI: https://doi.org/10.1007/s12275-023-00088-8
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95
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 Abstract
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Since the 2000s, the Y439 lineage of H9N2 avian influenza virus (AIV) has been the predominant strain circulating in poultry
in Korea; however, in 2020, the Y280 lineage emerged and spread rapidly nationwide, causing large economic losses. To
prevent further spread and circulation of such viruses, rapid detection and diagnosis through active surveillance programs
are crucial. Here, we developed a novel H9 rRT-PCR assay that can detect a broad range of H9Nx viruses in situations
in which multiple lineages of H9 AIVs are co-circulating. We then evaluated its efficacy using a large number of clinical
samples. The assay, named the Uni Kor-H9 assay, showed high sensitivity for Y280 lineage viruses, as well as for the Y439
lineage originating in Korean poultry and wild birds. In addition, the assay showed no cross-reactivity with other subtypes
of AIV or other avian pathogens. Furthermore, the Uni Kor-H9 assay was more sensitive, and had higher detection rates,
than reference H9 rRT-PCR methods when tested against a panel of domestically isolated H9 AIVs. In conclusion, the novel
Uni Kor-H9 assay enables more rapid and efficient diagnosis than the “traditional” method of virus isolation followed by
subtyping RT-PCR. Application of the new H9 rRT-PCR assay to AI active surveillance programs will help to control and
manage Korean H9 AIVs more efficiently.
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Citations
Citations to this article as recorded by  - Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses
Se-Hee An, Na-Yeong Kim, Gyeong-Beom Heo, Yong-Myung Kang, Youn-Jeong Lee, Kwang-Nyeong Lee
Journal of Virological Methods.2024; 327: 114942. CrossRef
- Transmissibility and pathogenicity of SARS-CoV-2 variants in animal models
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Young-Il Kim , Mark Anthony B. Casel , Young Ki Choi
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J. Microbiol. 2022;60(3):255-267. Published online March 2, 2022
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DOI: https://doi.org/10.1007/s12275-022-2033-z
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107
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11
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9
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Abstract
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As of February 2022, SARS-CoV-2 is still one of the most
serious public health threats due to its high mortality rate and
rapid spread of novel variants. Since the first outbreak in 2019,
general understanding of SARS-CoV-2 has been improved
through basic and clinical studies; however, knowledge gaps
still exist in our understanding of the emerging novel SARSCoV-
2 variants, which impacts the corresponding development
of vaccines and therapeutics. Especially, accumulation of
mutations in SARS-CoV-2 and rapid spread in populations
with previous immunity has resulted in selection of variants
that evade the host immune response. This phenomenon threatens
to render current SARS-CoV-2 vaccines ineffective for
controlling the pandemic. Proper animal models are essential
for detailed investigations into the viral etiology, transmission
and pathogenesis mechanisms, as well as evaluation of the
efficacy of vaccine candidates against recent SARS-CoV-2
variants. Further, the choice of animal model for each research
topic is important for researchers to gain better knowledge
of recent SARS-CoV-2 variants. Here, we review the advantages
and limitations of each animal model, including mice,
hamsters, ferrets, and non-human primates, to elucidate variant
SARS-CoV-2 etiology and transmission and to evaluate
therapeutic and vaccine efficacy.
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Citations
Citations to this article as recorded by - In vivo characterization of ACE2 expression in Sprague-Dawley rats and cultured primary brain pericytes highlights the utility of Rattus norvegicus in the study of COVID-19 brain pathophysiology
Eugene Park, Elaine Liu, Andrew J. Baker
Brain Research.2025; 1848: 149333. CrossRef - Utilizing non‐human primate models to combat recent COVID‐19/SARS‐CoV‐2 and viral infectious disease outbreaks
Taeho Kwon
Journal of Medical Primatology.2024;[Epub] CrossRef - Early detection of highly transmissible viral variants using phylogenomics
Michael R. May, Bruce Rannala
Science Advances.2024;[Epub] CrossRef - Animal Models, Zoonotic Reservoirs, and Cross-Species Transmission of Emerging Human-Infecting Coronaviruses
Yakhouba Kane, Gary Wong, George F. Gao
Annual Review of Animal Biosciences.2023; 11(1): 1. CrossRef - SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets
Elizabeth E. Zumbrun, Samantha E. Zak, Eric D. Lee, Philip A. Bowling, Sara I. Ruiz, Xiankun Zeng, Jeffrey W. Koehler, Korey L. Delp, Russel R. Bakken, Shannon S. Hentschel, Holly A. Bloomfield, Keersten M. Ricks, Tamara L. Clements, April M. Babka, John
Viruses.2023; 15(12): 2341. CrossRef - The Isolation and In Vitro Differentiation of Primary Fetal Baboon Tracheal Epithelial Cells for the Study of SARS-CoV-2 Host-Virus Interactions
Bharathiraja Subramaniyan, Sunam Gurung, Manish Bodas, Andrew R. Moore, Jason L. Larabee, Darlene Reuter, Constantin Georgescu, Jonathan D. Wren, Dean A. Myers, James F. Papin, Matthew S. Walters
Viruses.2023; 15(4): 862. CrossRef - Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef - Two years of COVID-19 pandemic: where are we now?
Jinjong Myoung
Journal of Microbiology.2022; 60(3): 235. CrossRef - SARS CoV-2 (Delta Variant) Infection Kinetics and Immunopathogenesis in Domestic Cats
Miruthula Tamil Selvan, Sachithra Gunasekara, Ping Xiao, Kristen Griffin, Shannon R. Cowan, Sai Narayanan, Akhilesh Ramachandran, Darren E. Hagen, Jerry W. Ritchey, Jennifer M. Rudd, Craig A. Miller
Viruses.2022; 14(6): 1207. CrossRef
- Antiviral effects of human placenta hydrolysate (Laennec) against SARS-CoV-2 in vitro and in the ferret model
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Eun-Ha Kim , Young-il Kim , Seung-Gyu Jang , Minju Im , Kyeongsoo Jeong , Young Ki Choi , Hae-Jung Han
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J. Microbiol. 2021;59(11):1056-1062. Published online October 6, 2021
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DOI: https://doi.org/10.1007/s12275-021-1367-2
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83
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8
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7
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Abstract
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The COVID-19 pandemic has caused unprecedented health,
social, and economic crises worldwide. However, to date, there
is an only a limited effective treatment for this disease. Human
placenta hydrolysate (hPH) has previously been shown to be
safe and to improve the health condition in patients with hyperferritinemia
and COVID-19. In this study, we aimed to
determine the antiviral effects of hPH against SARS-CoV-2
in vitro and in vivo models and compared with Remdesivir,
an FDA-approved drug for COVID-19 treatment. To assess
whether hPH inhibited SARS-CoV-2 replication, we determined
the CC50, EC50, and selective index (SI) in Vero cells
by infection with a SARS-CoV-2 at an MOI of 0.01. Further,
groups of ferrets infected with 105.8 TCID50/ml of SARS-CoV-2
and treated with hPH at 2, 4, 6 dpi, and compared their clinical
manifestation and virus titers in respiratory tracts with
PBS control-treated group. The mRNA expression of immunerelated
cytokines was determined by qRT-PCR. hPH treatment
attenuated virus replication in a dose-dependent manner in
vitro. In a ferret infection study, treatment with hPH resulted
in minimal bodyweight loss and attenuated virus replication
in the nasal wash, turbinates, and lungs of infected ferrets.
In addition, qRT-PCR results revealed that the hPH treatment
remarkably upregulated the gene expression of type I
(IFN-α and IFN-β) and II (IFN-γ) IFNs in SARS-CoV-2 infected
ferrets. Our data collectively suggest that hPH has antiviral
efficacy against SARS-CoV-2 and might be a promising
therapeutic agent for the treatment of SARS-CoV-2 infection.
-
Citations
Citations to this article as recorded by - Perinatal Hypoxia and Immune System Activation in Schizophrenia Pathogenesis: Critical Considerations During COVID-19 Pandemic
I Kawikova, K Hakenova, M Lebedeva, L Kleteckova, L Jakob, V Spicka, L Wen, F Spaniel, K Vales
Physiological Research.2024; : S615. CrossRef - Human Placenta Extract (HPH) Suppresses Inflammatory Responses in TNF-α/IFN-γ-Stimulated HaCaT Cells and a DNCB Atopic Dermatitis (AD)-Like Mouse Model
Jung Ok Lee, Youna Jang, A Yeon Park, Jung Min Lee, Kyeongsoo Jeong, So-Hyun Jeon, Hui Jin, Minju Im, Jae-Won Kim, Beom Joon Kim
Journal of Microbiology and Biotechnology.2024; 34(10): 1969. CrossRef - Systematic analysis of the pharmacology of standardized extracts of human placenta
T. E. Bogacheva, I. Yu. Torshin, O. A. Gromova
Pharmacokinetics and Pharmacodynamics.2024; (4): 3. CrossRef - Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef - Current state-of-the-art and potential future therapeutic drugs against COVID-19
Ailong Sha, Yi Liu, Haiyan Hao
Frontiers in Cell and Developmental Biology.2023;[Epub] CrossRef - SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets
Elizabeth E. Zumbrun, Samantha E. Zak, Eric D. Lee, Philip A. Bowling, Sara I. Ruiz, Xiankun Zeng, Jeffrey W. Koehler, Korey L. Delp, Russel R. Bakken, Shannon S. Hentschel, Holly A. Bloomfield, Keersten M. Ricks, Tamara L. Clements, April M. Babka, John
Viruses.2023; 15(12): 2341. CrossRef - Human placenta hydrolysates: from V.P. Filatov to the present day: Review
Olga A. Gromova, Ivan Yu. Torshin, Alexander G. Chuchalin, Valeriy А. Maximov
Terapevticheskii arkhiv.2022; 94(3): 434. CrossRef
- Differences in seroprevalence between epicenter and non-epicenter areas of the COVID-19 outbreak in South Korea
-
Hye Won Jeong , Hyun-Ha Chang , Eun Ji Kim , Yu Kyung Kim , Se-Mi Kim , Eun-Ha Kim , Young-Il Kim , Mark Anthony B. Casel , Seong-Gyu Kim , Rare Rollon , Seung-Gyu Jang , Kwang-Min Yu , Hee-Sung Kim , Hee Sue Park , Su-Jin Park , Yong-Dae Kim , Eung-Gook Kim , Young Ki Choi
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J. Microbiol. 2021;59(5):530-533. Published online April 28, 2021
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DOI: https://doi.org/10.1007/s12275-021-1095-7
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81
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2
Web of Science
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2
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Abstract
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To compare the standardized severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) seroprevalence of high
epicenter region with non-epicenter region, serological studies
were performed with a total of 3,268 sera from Daegu City
and 3,981 sera from Chungbuk Province. Indirect immunofluorescence
assay (IFA) for SARS-CoV-2 IgG results showed
a high seroprevalence rate in the Daegu City (epicenter) compared
with a non-epicenter area (Chungbuk Province) (1.27%
vs. 0.91%, P = 0.0358). It is noteworthy that the highest seroprevalence
in Daegu City was found in elderly patients (70’s)
whereas young adult patients (20’s) in Chungbuk Province
showed the highest seroprevalence. Neutralizing antibody
(NAb) titers were found in three samples from Daegu City
(3/3, 268, 0.09%) while none of the samples from Chungbuk
Province were NAb positive. These results demonstrated that
even following the large outbreak, the seropositive rate of
SARS-CoV-2 in the general population remained low in
South Korea.
-
Citations
Citations to this article as recorded by - Distinctive Combinations of RBD Mutations Contribute to Antibody Evasion in the Case of the SARS-CoV-2 Beta Variant
Tae-Hun Kim, Sojung Bae, Sunggeun Goo, Jinjong Myoung
Journal of Microbiology and Biotechnology.2023; 33(12): 1587. CrossRef - The Seroprevalence of SARS-CoV-2 in Children During Early COVID-19 Pandemic in Korea: A Nationwide, Population-Based Study
Jin Lee, Young June Choe, Dohsik Minn, Jong-Hyun Kim
Journal of Korean Medical Science.2022;[Epub] CrossRef
- Development of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) thermal inactivation method with preservation of diagnostic sensitivity
-
Young-Il Kim , Mark Anthony B. Casel , Se-Mi Kim , Seong-Gyu Kim , Su-Jin Park , Eun-Ha Kim , Hye Won Jeong , Haryoung Poo , Young Ki Choi
-
J. Microbiol. 2020;58(10):886-891. Published online September 29, 2020
-
DOI: https://doi.org/10.1007/s12275-020-0335-6
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97
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24
Web of Science
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26
Crossref
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Abstract
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Various treatments and agents had been reported to inactivate
RNA viruses. Of these, thermal inactivation is generally
considered an effective and cheap method of sample
preparation for downstream assays. The purpose of this study
is to establish a safe inactivation method for SARS-CoV-2
without compromising the amount of amplifiable viral genome
necessary for clinical diagnoses. In this study, we demonstrate
the infectivity and genomic stability of SARSCoV-
2 by thermal inactivation at both 56°C and 65°C. The
results
substantiate that viable SARS-CoV-2 is readily inactivated
when incubated at 56°C for 30 min or at 65°C for
10 min. qRT-PCR of specimens heat-inactivated at 56°C for
30 min or 65°C for 15 min revealed similar genomic RNA
stability compared with non-heat inactivated specimens. Further,
we demonstrate that 30 min of thermal inactivation at
56°C could inactivate viable viruses from clinical COVID-19
specimens without attenuating the qRT-PCR diagnostic sensitivity.
Heat treatment of clinical specimens from COVID-19
patients at 56°C for 30 min or 65°C for 15 min could be a useful
method
for the inactivation of a highly contagious agent,
SARS-CoV-2. Use of this method would reduce the potential
for secondary infections in BSL2 conditions during diagnostic
procedures. Importantly, infectious virus can be inactivated
in clinical specimens without compromising the
sensitivity of the diagnostic RT-PCR assay.
-
Citations
Citations to this article as recorded by - Evaluating Interlaboratory Variability in Wastewater-Based COVID-19 Surveillance
Arianna Azzellino, Laura Pellegrinelli, Ramon Pedrini, Andrea Turolla, Barbara Bertasi, Sandro Binda, Sara Castiglioni, Clementina E. Cocuzza, Fabio Ferrari, Andrea Franzetti, Maria Giovanna Guiso, Marina Nadia Losio, Marianna Martinelli, Antonino Martine
Microorganisms.2025; 13(3): 526. CrossRef - Electrochemical genomagnetic assay for detection of SARS-CoV-2 RNA using a disposable microfluidic platform
Daniel Júnior Almeida dos Santos, Tássia Regina de Oliveira, Henrique Pott-Junior, Matias Eliseo Melendez, Ester Cerdeira Sabino, Ronaldo Censi Faria
Talanta.2025; 294: 128186. CrossRef - Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air
Tomoyo Yoshinaga, Yoshinori Ando, Yumi Sato, Takeru Kishida, Masaaki Kitajima
Scientific Reports.2025;[Epub] CrossRef - Establishment of national standards of SARS-CoV-2 variants in Taiwan
Ming-Sian Wu, Pu-Chieh Chang, Po-Lin Lin, Chun-Hsi Tso, Hsin-Mei Chen, Yi-Hsuan Peng, Po-Chih Wu, Jia-Chuan Hsu, Der-Yuan Wang
Heliyon.2024; 10(19): e38275. CrossRef - EU surveys insights: analytical tools, future directions, and the essential requirement for reference materials in wastewater monitoring of SARS-CoV-2, antimicrobial resistance and beyond
Valentina Paracchini, Mauro Petrillo, Anandasagari Arcot Rajashekar, Piotr Robuch, Ursula Vincent, Philippe Corbisier, Simona Tavazzi, Barbara Raffael, Elisabetta Suffredini, Giuseppina La Rosa, Bernd Manfred Gawlik, Antonio Marchini
Human Genomics.2024;[Epub] CrossRef - Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection
Natalia Capriotti, Leslie C. Amorós Morales, Elisa de Sousa, Luciana Juncal, Matias Luis Pidre, Lucila Traverso, Maria Florencia López, Maria Leticia Ferelli, Gabriel Lavorato, Cristian Lillo, Odin Vazquez Robaina, Nicolas Mele, Carolina Vericat, Patricia
Heliyon.2024; 10(3): e25377. CrossRef - Validating the inactivation of viral pathogens with a focus on SARS-CoV-2 to safely transfer samples from high-containment laboratories
Sankar Prasad Chaki, Melissa M. Kahl-McDonagh, Benjamin W. Neuman, Kurt A. Zuelke
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - COPMAN: A novel high-throughput and highly sensitive method to detect viral nucleic acids including SARS-CoV-2 RNA in wastewater
Yuka Adachi Katayama, Shin Hayase, Yoshinori Ando, Tomohiro Kuroita, Kazuya Okada, Ryo Iwamoto, Toru Yanagimoto, Masaaki Kitajima, Yusaku Masago
Science of The Total Environment.2023; 856: 158966. CrossRef - Sputum handling for rheology
Lydia Esteban Enjuto, Matthieu Robert de Saint Vincent, Max Maurin, Bruno Degano, Hugues Bodiguel
Scientific Reports.2023;[Epub] CrossRef - A novel strategy to avoid sensitivity loss in pooled testing for SARS-CoV-2 surveillance: validation using nasopharyngeal swab and saliva samples
Georgia G. Millward, Shane M. Popelka, Anthony G. Gutierrez, William J. Kowallis, Robert L. von Tersch, Subrahmanyam V. Yerramilli
Frontiers in Public Health.2023;[Epub] CrossRef - The Biosafety Research Road Map: The Search for Evidence to Support Practices in the Laboratory—SARS-CoV-2
Stuart D. Blacksell, Sandhya Dhawan, Marina Kusumoto, Kim Khanh Le, Kathrin Summermatter, Joseph O'Keefe, Joseph Kozlovac, Salama Suhail Almuhairi, Indrawati Sendow, Christina M. Scheel, Anthony Ahumibe, Zibusiso M. Masuku, Kazunobu Kojima, David R. Harpe
Applied Biosafety.2023; 28(2): 87. CrossRef - Comparative Performance of Serological (IgM/IgG) and Molecular Testing (RT-PCR) of COVID-19 in Three Private Universities in Cameroon during the Pandemic
Rodrigue Kamga Wouambo, Cecile Ingrid Djuikoué, Livo Forgu Esemu, Luc Aime Kagoue Simeni, Murielle Chantale Tchitchoua, Paule Dana Djouela Djoulako, Joseph Fokam, Madeleine Singwe-Ngandeu, Eitel Mpoudi Ngolé, Teke Apalata
Viruses.2023; 15(2): 407. CrossRef - Molecular test for COVID-19 diagnosis based on a colorimetric genomagnetic assay
Tássia Regina de Oliveira, Taíse Helena Oliveira Leite, Wyllian Neves Miranda, Erika Regina Manuli, Fábio Leal, Ester Sabino, Henrique Pott-Junior, Matias Melendez, Ronaldo Censi Faria
Analytica Chimica Acta.2023; 1257: 341167. CrossRef - Methods of Inactivation of Highly Pathogenic Viruses for Molecular, Serology or Vaccine Development Purposes
Simon Elveborg, Vanessa Monteil, Ali Mirazimi
Pathogens.2022; 11(2): 271. CrossRef - A collaborative study to establish the national standard for SARS-CoV-2 RNA nucleic acid amplification techniques (NAAT) in Taiwan
Po-Lin Lin, Ming-Sian Wu, Po-Chi Wu, Hsin-Mei Chen, Yi-Hsuan Peng, Jia-Chuan Hsu, Der-Yuan Wang
Biologicals.2022; 79: 31. CrossRef - COVID-19 diagnosis by SARS-CoV-2 Spike protein detection in saliva using an ultrasensitive magneto-assay based on disposable electrochemical sensor
Evair D. Nascimento, Wilson T. Fonseca, Tássia R. de Oliveira, Camila R.S.T.B. de Correia, Vitor M. Faça, Beatriz P. de Morais, Virginia C. Silvestrini, Henrique Pott-Junior, Felipe R. Teixeira, Ronaldo C. Faria
Sensors and Actuators B: Chemical.2022; 353: 131128. CrossRef - Use of MALDI-TOF mass spectrometry for virus identification: a review
Tomas Do, Roman Guran, Vojtech Adam, Ondrej Zitka
The Analyst.2022; 147(14): 3131. CrossRef - COPMAN: A Novel High-Throughput and Highly Sensitive Method to Detect Viral Nucleic Acids Including SARS-CoV-2 RNA in Wastewater
Yuka Adachi Katayama, Shin Hayase, Yoshinori Ando, Tomohiro Kuroita, Kazuya Okada, Ryo Iwamoto, Toru Yanagimoto, Masaaki Kitajima, Yusaku Masago
SSRN Electronic Journal .2022;[Epub] CrossRef - Effect of heat inactivation for the detection of severe acute respiratory syndrome-corona virus-2 (SARS-CoV-2) with reverse transcription real time polymerase chain reaction (rRT-PCR): evidence from Ethiopian study
Belete Woldesemayat, Gebremedihin Gebremicael, Kidist Zealiyas, Amelework Yilma, Sisay Adane, Mengistu Yimer, Gadissa Gutema, Altaye Feleke, Kassu Desta
BMC Infectious Diseases.2022;[Epub] CrossRef - Evaluation of the SARS-CoV-2 Inactivation Efficacy Associated With Buffers From Three Kits Used on High-Throughput RNA Extraction Platforms
Ruth E. Thom, Lin S. Eastaugh, Lyn M. O’Brien, David O. Ulaeto, James S. Findlay, Sophie J. Smither, Amanda L. Phelps, Helen L. Stapleton, Karleigh A. Hamblin, Simon A. Weller
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef - Viral Inactivation Impacts Microbiome Estimates in a Tissue-Specific Manner
Alba Boix-Amorós, Enrica Piras, Kevin Bu, David Wallach, Matthew Stapylton, Ana Fernández-Sesma, Dolores Malaspina, Jose C. Clemente, Ileana M. Cristea
mSystems.2021;[Epub] CrossRef - Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples
Stephany D. Villota, Victoria E. Nipaz, Andrés Carrazco-Montalvo, Sarah Hernandez, Jesse J. Waggoner, Patricio Ponce, Josefina Coloma, Alberto Orlando, Varsovia Cevallos
Journal of Virological Methods.2021; 298: 114302. CrossRef - Comparison of the Modified Centers for Disease Control and Prevention 2019-Novel Coronavirus Real-Time RT-PCR Method for Detection of Infectious and Heat-Inactivated Virus on Stainless Steel
Sharon L Brunelle, Patrick M Bird, Jeremy Boone, Maria Nelson, Zerlinde Johnson, Scott Coates
Journal of AOAC INTERNATIONAL.2021; 104(4): 867. CrossRef - Heat-Treated Virus Inactivation Rate Depends Strongly on Treatment Procedure: Illustration with SARS-CoV-2
Amandine Gamble, Robert J. Fischer, Dylan H. Morris, Claude Kwe Yinda, Vincent J. Munster, James O. Lloyd-Smith, Christopher A. Elkins
Applied and Environmental Microbiology.2021;[Epub] CrossRef - Comparison of Seven Commercial Severe Acute Respiratory Syndrome Coronavirus 2 Nucleic Acid Detection Reagents with Pseudovirus as Quality Control Material
Ying Yan, Le Chang, Wenxin Luo, Junyi Liu, Fei Guo, Lunan Wang
The Journal of Molecular Diagnostics.2021; 23(3): 300. CrossRef - Evaluation of the persistence of SARS-CoV-2 (ATCC® VR-1986HK™) on two different food contact materials: flow pack polyethylene and polystyrene food trays
Marta Castrica, Claudia Balzaretti, Dino Miraglia, Patrizio Lorusso, Annamaria Pandiscia, Giuseppina Tantillo, Francesca Romana Massacci, Valentina Terio
LWT.2021; 146: 111606. CrossRef
- Efficacy of A/H1N1/2009 split inactivated influenza A vaccine (GC1115) in mice and ferrets
-
Hae Jung Han , Min-Suk Song , Su-Jin Park , Han Yeul Byun , Norbert John C. Robles , Suk-Hoon Ha , Young Ki Choi
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J. Microbiol. 2019;57(2):163-169. Published online January 31, 2019
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DOI: https://doi.org/10.1007/s12275-019-8504-1
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71
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4
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4
Crossref
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Abstract
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To evaluate the efficacy of a non-adjuvant A/H1N1/2009 influenza
A vaccine (GC1115), we demonstrated the immunogenicity
and protective efficacy of GC1115 in mouse and
ferret models. The immunogenicity of GC1115 was confirmed
after intramuscular administration of 1.875, 3.75, 7.5, and
15 μg hemagglutinin antigen (HA) in mice and 7.5, 15, and
30 μg HA in ferrets at 3-week intervals. A single immunization
with GC1115 at HA doses > 7.5 μg induced detectable
seroconversion in most mice, and all mice given a second
dose exhibited high antibody responses in a dose-dependent
manner. The mice in the mock (PBS) and 1.875 μg HA immunized
groups succumbed by 13 days following A/California/
04/09 infection, while all mice in groups given more
than 3.75 μg HA were protected from lethal challenge with
the A/California/04/09 virus. In ferrets, although immunization
with even a single dose of 15 or 30 μg of HA induced
detectable HI antibodies, all ferrets given two doses of vaccine
seroconverted and exhibited HI titers greater than 80
units. Following challenge with A/California/04/09, the mock
(PBS) immunized ferrets showed influenza-like clinical symptoms,
such as increased numbers of coughs, elevated body
temperature, and body weight loss, for 7 days, while GC1115-
immunized ferrets showed attenuated clinical symptoms only
for short time period (3–4 days). Further, GC1115-immunized
ferrets displayed significantly lower viral titers in the upper
respiratory tract (nasal cavity) than the mock vaccinated group
in a dose-dependent manner. Taken together, this study demonstrates
the immunogenicity and protective efficacy of
GC1115 as a non-adjuvanted vaccine.
-
Citations
Citations to this article as recorded by - Dose sparing enabled by immunization with influenza vaccine using orally dissolving film
Keon-Woong Yoon, Ki Back Chu, Gi-Deok Eom, Jie Mao, Su In Heo, Fu-Shi Quan
International Journal of Pharmaceutics.2024; 667: 124945. CrossRef - Ferrets as a model for tuberculosis transmission
Tuhina Gupta, Naveen Somanna, Thomas Rowe, Monica LaGatta, Shelly Helms, Simon Odera Owino, Tomislav Jelesijevic, Stephen Harvey, Wayne Jacobs, Thomas Voss, Kaori Sakamoto, Cheryl Day, Christopher Whalen, Russell Karls, Biao He, S. Mark Tompkins, Abhijeet
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - AddaVax Formulated with PolyI:C as a Potential Adjuvant of MDCK-based Influenza Vaccine Enhances Local, Cellular, and Antibody Protective Immune Response in Mice
Xuanxuan Nian, Jiayou Zhang, Tao Deng, Jing Liu, Zheng Gong, Chuanshuo Lv, Luyao Yao, Junying Li, Shihe Huang, Xiaoming Yang
AAPS PharmSciTech.2021;[Epub] CrossRef - The Intersection of Age and Influenza Severity: Utility of Ferrets for Dissecting the Age-Dependent Immune Responses and Relevance to Age-Specific Vaccine Development
Melissa Rioux, Magen E. Francis, Cynthia L. Swan, Anni Ge, Andrea Kroeker, Alyson A. Kelvin
Viruses.2021; 13(4): 678. CrossRef
- Molecular characterization of mammalian-adapted Korean-type avian H9N2 virus and evaluation of its virulence in mice
-
Kuk Jin Park , Min-Suk Song , Eun-Ha Kim , Hyeok-il Kwon , Yun Hee Baek , Eun-hye Choi , Su-Jin Park , Se Mi Kim , Young-il Kim , Won-Suk Choi , Dae-Won Yoo , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2015;53(8):570-577. Published online July 31, 2015
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DOI: https://doi.org/10.1007/s12275-015-5329-4
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72
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14
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Abstract
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Avian influenza A virus (AIV) is commonly isolated from
domestic poultry and wild migratory birds, and the H9N2
subtype is the most prevalent and the major cause of severe
disease in poultry in Korea. In addition to the veterinary concerns
regarding the H9N2 subtype, it is also considered to
be the next potential human pandemic strain due to its rapid
evolution and interspecies transmission. In this study, we
utilize serial lung-to-lung passage of a low pathogenic avian
influenza virus (LPAI) H9N2 (A/Ck/Korea/163/04, WT163)
(Y439-lineage) in mice to increase pathogenicity and investigate
the potential virulence marker. Mouse-adapted H9N2
virus obtained high virulence (100% mortality) in mice after
98 serial passages. Sequence results show that the mouse
adaptation (ma163) possesses several mutations within seven
gene segments (PB2, PA, HA, NP, NA, M, and NS) relative
to the wild-type strain. The HA gene showed the most mutations
(at least 11) with one resulting in the loss of an N-glycosylation
site (at amino acid 166). Moreover, reverse genetic
studies established that an E627K substitution in PB2 and the
loss of the N-glycosylation site in the HA protein (aa166) are
critical virulence markers in the mouse-adapted H9N2 virus.
Thus, these results add to the increasing body of mutational
analysis data defining the function of the viral polymerase
and HA genes and their roles in mammalian host adaptation.
To our knowledge, this is first report of the generation
of a mammalian-adapted Korea H9N2 virus (Y493-lineages).
Therefore, this study offers valuable insights into the molecular
evolution of the LPAI Korean H9N2 in a new host and
adds to the current knowledge of the molecular markers associated
with increased virulence.
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Citations
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Nature Communications.2023;[Epub] CrossRef - Current situation and control strategies of H9N2 avian influenza in South Korea
Mingeun Sagong, Kwang-Nyeong Lee, Eun-Kyoung Lee, Hyunmi Kang, Young Ki Choi, Youn-Jeong Lee
Journal of Veterinary Science.2023;[Epub] CrossRef - Antigenic Evolution Characteristics and Immunological Evaluation of H9N2 Avian Influenza Viruses from 1994–2019 in China
Qingzheng Liu, Lingcai Zhao, Yanna Guo, Yongzhen Zhao, Yingfei Li, Na Chen, Yuanlu Lu, Mengqi Yu, Lulu Deng, Jihui Ping
Viruses.2022; 14(4): 726. CrossRef - Molecular epidemiology and pathogenicity of H5N1 and H9N2 avian influenza viruses in clinically affected chickens on farms in Bangladesh
Ripatun Nahar Ripa, Joshua E. Sealy, Jayna Raghwani, Tridip Das, Himel Barua, Md. Masuduzzaman, A. K. M. Saifuddin, Md. Reajul Huq, Mohammad Inkeyas Uddin, Munir Iqbal, Ian Brown, Nicola S. Lewis, Dirk Pfeiffer, Guillaume Fournie, Paritosh Kumar Biswas
Emerging Microbes & Infections.2021; 10(1): 2223. CrossRef - Mouse adaptation of the H9N2 avian influenza virus causes the downregulation of genes related to innate immune responses and ubiquitin-mediated proteolysis in mice
Jing Guo, Xinxin Gao, Baotao Liu, Yubao Li, Wenqiang Liu, Jianbiao Lu, Cheng Liu, Rui Xue, Xuyong Li
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Scientific Reports.2019;[Epub] CrossRef - A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
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Min Kang, Hyung-Kwan Jang
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Won-Suk Choi, Khristine Kaith S. Lloren, Yun Hee Baek, Min-Suk Song
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Won-Suk Choi, Yun Hee Baek, Jin Jung Kwon, Ju Hwan Jeong, Su-Jin Park, Young-il Kim, Sun-Woo Yoon, Jungwon Hwang, Myung Hee Kim, Chul-Joong Kim, Richard J. Webby, Young Ki Choi, Min-Suk Song
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Se Mi Kim, Young-Il Kim, Su-Jin Park, Eun-Ha Kim, Hyeok-il Kwon, Young-Jae Si, In-Won Lee, Min-Suk Song, Young Ki Choi, Jae U. Jung
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Hanna Sediri, Swantje Thiele, Folker Schwalm, Gülsah Gabriel, Hans-Dieter Klenk
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- Adjuvant Efficacy of mOMV against Avian Influenza Virus Infection in Mice
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Byeong-Jae Lee , Sang-Ho Lee , Min-Suk Song , Philippe Noriel Q. Pascua , Hyeok-il Kwon , Su-Jin Park , Eun-Ha Kim , Arun Decano , Se Mi Kim , Gyo Jin Lim , Doo-Jin Kim , Kyu-Tae Chang , Sang-Hyun Kim , Young Ki Choi
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J. Microbiol. 2013;51(5):682-688. Published online October 31, 2013
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DOI: https://doi.org/10.1007/s12275-013-3411-3
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Abstract
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Highly pathogenic avian influenza H5N1 viruses are found chiefly in birds and have caused severe disease and death in infected humans. Development of influenza vaccines capable of inducing heterosubtypic immunity against a broad range of influenza viruses is the best option for the preparedness, since vaccination remains the principal method in controlling influenza viral infections. Here, a mOMV-adjuvanted recombinant H5N2 (rH5N2) whole virus antigen vaccine with A/Environment/Korea/W149/06(H5N1)-derived H5 HA and A/Chicken/Korea/ma116/04(H9N2)-derived N2 NA in the backbone of A/Puerto Rico/8/34(H1N1) was prepared and generated by reverse genetics. Groups of mice were vaccinated by a prime-boost regime with the rH5N2 vaccine (1.75 μg of HA with/without 10 μg mOMV or aluminum hydroxide adjuvant for comparison). At two weeks post-immunizations, vaccinated mice were challenged with lethal doses of 103.5 EID50/ml of H5N1 or H9N2 avian influenza viruses, and were monitored for 15 days. Both mOMV- and alum-adjuvant vaccine groups had high survival rates after H5N1 infection and low levels of body weight changes compared to control groups. Interestingly, the mOMV-adjuvanted group induced better cross-reactive antibody responses serologically and promoted cross-protectivity against H5N1 and H9N2 virus challenges. Our results suggest that mOMV could be used as a vaccine adjuvant in the development of effective vaccines used to control influenza A virus transmission.
- Sublingual Administration of Bacteria-Expressed Influenza Virus Hemagglutinin 1 (HA1) Induces Protection against Infection with 2009 Pandemic H1N1 Influenza Virus
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Byoung-Shik Shim , Jung-ah Choi , Ho-Hyun Song , Sung-Moo Park , In Su Cheon , Ji-Eun Jang , Sun Je Woo , Chung Hwan Cho , Min-Suk Song , Hyemi Kim , Kyung Joo Song , Jae Myun Lee , Suhng Wook Kim , Dae Sub Song , Young Ki Choi , Jae-Ouk Kim , Huan Huu Nguyen , Dong Wook Kim , Young Yil Bahk , Cheol-Heui Yun , Man Ki Song
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J. Microbiol. 2013;51(1):130-135. Published online March 2, 2013
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DOI: https://doi.org/10.1007/s12275-013-2399-z
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Abstract
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Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a wellestablished bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
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Citations
Citations to this article as recorded by -
Immunomodulatory Effects of Probiotic
Lactobacillus casei
On GM-CSF-adjuvanted Influenza Dna Vaccine
Mehran Mahooti, Elahe Abdolalipour, Behrokh Farahmand, Sadegh Shirian, Amir Ghaemi
Future Virology.2022; 17(9): 633. CrossRef - Structural Insights for Anti-Influenza Vaccine Design
Lifen Han, Cong Chen, Xianlin Han, Shujin Lin, Xiulan Ao, Xiao Han, Jianmin Wang, Hanhui Ye
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J. Reina
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Ailian Zhang, Danyang Wang, Jinyao Li, Feng Gao, Xucheng Fan, Farhat Afrin
PLOS ONE.2017; 12(8): e0183720. CrossRef - HA1-2-fljB Vaccine Induces Immune Responses against Pandemic Swine-Origin H1N1 Influenza Virus in Mice
Xilong Kang, Yun Yang, Yang Jiao, Hongqin Song, Li Song, Dan Xiong, Lili Wu, Zhiming Pan, Xinan Jiao
Microbial Physiology.2016; 26(6): 422. CrossRef - A De-O-acylated Lipooligosaccharide-Based Adjuvant System Promotes Antibody and Th1-Type Immune Responses to H1N1 Pandemic Influenza Vaccine in Mice
Ji In Ryu, Shin Ae Park, Seo Ri Wui, Ara Ko, Ji Eun Han, Jung Ah Choi, Man Ki Song, Kwang Sung Kim, Yang Je Cho, Na Gyong Lee
BioMed Research International.2016; 2016: 1. CrossRef - A Lipopolysaccharide from Pantoea Agglomerans Is a Promising Adjuvant for Sublingual Vaccines to Induce Systemic and Mucosal Immune Responses in Mice via TLR4 Pathway
Masahiro Fukasaka, Daisuke Asari, Eiji Kiyotoh, Arimichi Okazaki, Yasuyuki Gomi, Takeshi Tanimoto, Osamu Takeuchi, Shizuo Akira, Mitsuhiko Hori, John S Tregoning
PLOS ONE.2015; 10(5): e0126849. CrossRef - Methylglycol chitosan and a synthetic TLR4 agonist enhance immune responses to influenza vaccine administered sublingually
Justin L. Spinner, Hardeep S. Oberoi, Yvonne M. Yorgensen, Danielle S. Poirier, David J. Burkhart, Martin Plante, Jay T. Evans
Vaccine.2015; 33(43): 5845. CrossRef - Comparative analysis of antibody induction and protection against influenza virus infection by DNA immunization with HA, HAe, and HA1 in mice
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Archives of Virology.2014; 159(4): 689. CrossRef - Sublingual Delivery of Vaccines for the Induction of Mucosal Immunity
Byoung-Shik Shim, Youngjoo Choi, In Su Cheon, Man Ki Song
Immune Network.2013; 13(3): 81. CrossRef - Mucosal Vaccination with Recombinant Adenovirus Encoding Nucleoprotein Provides Potent Protection against Influenza Virus Infection
So-Hee Kim, Joo Young Kim, Youngjoo Choi, Huan H. Nguyen, Man Ki Song, Jun Chang, Eui-Cheol Shin
PLoS ONE.2013; 8(9): e75460. CrossRef - Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system
Ha‐Na Na, Kyung Hyun Kim, Man Ki Song, Hye‐lim Park, Eun‐young Lee, Seung‐Hyun Shim, Sooho Park, Jae‐Hwan Nam
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- ERRATUM] Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
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Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2012;50(4):715-715.
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Abstract
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In the article by Song et al. that appears in the Journal of Microbiology 2012; 50, 478-488. Page 478, the name of 7th author, Kyu-Jin Woo, should read as Gyu-Jin Woo.
- Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
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Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
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J. Microbiol. 2012;50(3):487-488.
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Abstract
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The threat of a highly pathogenic avian influenza (HPAI) H5N1 virus causing the next pandemic remains a major concern. In this study, we evaluated the immunogenicity and efficacy of an inactivated whole-virus H5N1 pre-pandemic vaccine (MG1109) formulated by Green Cross Co., Ltd containing the hemagglutinin (HA) and neuraminidase (NA) genes of the clade 1 A/Vietnam/1194/04 virus in the backbone of A/Puerto Rico/8/34 (RgVietNam/04xPR8/34). Administration of the MG1109 vaccine (2-doses) in mice and ferrets elicited high HI and SN titers in a dose-dependent manner against the homologous (RgVietNam/04xPR8/34) and various heterologous H5N1 strains, (RgKor/W149/06xPR8/34, RgCambodia/04xPR8/34, RgGuangxi/05xPR8/34), including a heterosubtypic H5N2 (A/Aquatic bird/orea/W81/05) virus. However, efficient cross-reactivity was not observed against heterosubtypic H9N2 (A/Ck/Korea/H0802/08) and H1N1 (PR/8/34) viruses. Mice immunized with 1.9 μg HA/dose of MG1109 were completely protected from lethal challenge with heterologous wild-type HPAI H5N1 A/EM/Korea/W149/06 (clade 2.2) and mouse-adapted H5N2 viruses. Furthermore, ferrets administered at least 3.8 μg HA/dose efficiently suppressed virus growth in the upper respiratory tract and lungs. Vaccinated mice and ferrets also demonstrated attenuation of clinical disease signs and limited virus spread to other organs. Thus, this vaccine provided immunogenic responses in mouse and ferret models even against challenge with heterologous HPAI H5N1 and H5N2 viruses. Since the specific strain of HPAI H5N1 virus that would potentially cause the next outbreak is unknown, pre-pandemic vaccine preparation that could provide crossprotection against various H5 strains could be a useful approach in the selection of promising candidate vaccines in the future.
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