Journal of Microbiology

Soo-Jin Yeom

1 Article

[MINIREVIEW]Phosphate sugar isomerases and their potential for rare sugar bioconversion: Soo-Jung Kim , Yeong-Su Kim , Soo-Jin Yeom; J. Microbiol. 2020;58(9):725-733. Published online June 25, 2020; DOI: https://doi.org/10.1007/s12275-020-0226-x

83 View
0 Download
8 Web of Science
9 Crossref

Phosphate sugar isomerases, catalyzing the isomerization between ketopentose/ketohexose phosphate and aldopentose/ aldohexose phosphate, play an important role in microbial sugar metabolism. They are present in a wide range of microorganisms. They have attracted increasing research interest because of their broad substrate specificity and great potential in the enzymatic production of various rare sugars. Here, the enzymatic properties of various phosphate sugar isomerases are reviewed in terms of their substrate specificities and their applications in the production of valuable rare sugars because of their functions such as low-calorie sweeteners, bulking agents, and pharmaceutical precursor. Specifically, we focused on the industrial applications of D-ribose-5-phosphate isomerase and D-mannose-6-phosphate isomerase to produce D-allose and L-ribose, respectively.

Citations

Citations to this article as recorded by

Transforming monosaccharides: Recent advances in rare sugar production and future exploration
Shin-ichi Nakakita, Jun Hirabayashi
BBA Advances.2025; 7: 100143. CrossRef
Production of Value-Added Arabinofuranosyl Nucleotide Analogues from Nucleoside by an In Vitro Enzymatic Synthetic Biosystem
Yuxue Liu, Xiaojing Zhang, Erchu Yang, Xiaobei Liu, Weiwei Su, Zhenyu Wang, Hailei Wang
Biomolecules.2024; 14(11): 1440. CrossRef
Taxonomic Identification of the Arctic Strain Nocardioides Arcticus Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress
Bailin Cong, Hui Zhang, Shuang Li, Shenghao Liu, Jing Lin, Aifang Deng, Wenqi Liu, Yan Yang
International Journal of Molecular Sciences.2023; 24(18): 13943. CrossRef
A putative glucose 6-phosphate isomerase has pleiotropic functions on virulence and other mechanisms in Acidovorax citrulli
Lynn Heo, Yoobin Han, Yongmin Cho, Junhyeok Choi, Jeongwook Lee, Sang-Wook Han
Frontiers in Plant Science.2023;[Epub] CrossRef
Assessing the genomic composition, putative ecological relevance and biotechnological potential of plasmids from sponge bacterial symbionts
Vanessa Oliveira, Ana R.M. Polónia, Daniel F.R. Cleary, Yusheng M. Huang, Nicole J. de Voogd, Tina Keller-Costa, Rodrigo Costa, Newton C.M. Gomes
Microbiological Research.2022; 265: 127183. CrossRef
Enhanced isomerization of rare sugars by ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1
Rong Wang, Xinqi Xu, Xuemei Yao, Hengtao Tang, Xin Ju, Liangzhi Li
Enzyme and Microbial Technology.2021; 148: 109789. CrossRef
Simultaneous Production of D-Tagatose, D-Arabitol and Galactitol from Cheese Whey Powder Using Combined Biotransformation and Fermentation Strategies
Guoyan Zhang, Hossain M. Zabed, Yingfeng An, Junhua Yun, Jiaqi Huang, Yufei Zhang, Xiaolan Li, Jiangfei Wang, Xianghui Qi
SSRN Electronic Journal .2021;[Epub] CrossRef
Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst
Hengtao Tang, Xin Ju, Jing Zhao, Liangzhi Li
Applied Microbiology and Biotechnology.2021; 105(2): 509. CrossRef
Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol
Mesfin Angaw Tesfay, Xin Wen, Yujie Liu, Huibin Lin, Linxu Chen, Jianqiang Lin, Jianqun Lin
Bioprocess and Biosystems Engineering.2021; 44(6): 1021. CrossRef

Soo-Jin Yeom

1 Article

Investigation of Bottleneck Enzyme Through Flux Balance Analysis to Improve Glycolic Acid Production in Escherichia coli: Jungyeon Kim, Ye-Bin Kim, Ju-Young Kim, Min-Ju Seo, Soo-Jin Yeom, Bong Hyun Sung; J. Microbiol. 2024;62(11):1023-1033. Published online October 28, 2024; DOI: https://doi.org/10.1007/s12275-024-00175-4

108 View
0 Download
1 Web of Science
1 Scopus

Abstract: Amid rising environmental concerns, attempts have been made to produce glycolic acid (GA) using microbial processes with renewable carbon resources instead of using chemicals. The Dahms pathway for GA production uses xylose as a substrate and consists of relatively simple enzymatic steps. However, employing it leads to a decrease in cell growth and GA productivity. Systematically identifying and addressing metabolic bottlenecks in the Dahms pathway are essential for efficient glycolic acid (GA) production have not yet been performed. Through metabolic flux balance analysis, we found that insufficient aldehyde dehydrogenase (AldA) activity lowers GA production and negatively affects cell growth due to reduced energy production. Thus, we discovered a novel AldA isolated from Buttiauxella agrestis (BaAldA) demonstrated a 1.69-fold lower K_M and a 1.49-fold higher turnover rate (k_cat/K_M) than AldA from Escherichia coli (EcAldA). GA production in E. coli harboring BaAldA was 1.59 times higher than in the original strain. Fed-batch fermentation of E. coli harboring BaAldA produced 22.70 g/L GA with a yield of 0.497 g/g_xylose (98.2% of the theoretical maximum yield in the Dahms pathway), showing a higher final yield for GA than previously reported in E. coli. Our novel BaAldA enzyme shows great potential for the production of GA using microorganisms or enzymes. Furthermore, our approach to identifying metabolic bottlenecks using flux balance analysis could be utilized to enhance the microbial production of various desirable products in future studies.

Author index