Journal of Microbiology

Sangyong Lim

10 Articles

Functional Characterization of DNA N‑Glycosylase Ogg1 and Ntg1 in DNA Damage Stress of Cryptococcus neoformans: Kwang-Woo Jung , Sunhak Kwon , Jong-Hyun Jung , Sangyong Lim , Yong-Sun Bahn; J. Microbiol. 2023;61(11):981-992. Published online December 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00092-y

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Reactive oxygen species induce DNA strand breaks and DNA oxidation. DNA oxidation leads to DNA mismatches, resulting in mutations in the genome if not properly repaired. Homologous recombination (HR) and non-homologous end-joining (NHEJ) are required for DNA strand breaks, whereas the base excision repair system mainly repairs oxidized DNAs, such as 8-oxoguanine and thymine glycol, by cleaving the glycosidic bond, inserting correct nucleotides, and sealing the gap. Our previous studies revealed that the Rad53-Bdr1 pathway mainly controls DNA strand breaks through the regulation of HRand NHEJ-related genes. However, the functional roles of genes involved in the base excision repair system remain elusive in Cryptococcus neoformans. In the present study, we identified OGG1 and NTG1 genes in the base excision repair system of C. neoformans, which are involved in DNA oxidation repair. The expression of OGG1 was induced in a Hog1-dependent manner under oxidative stress. On the other hand, the expression of NTG1 was strongly induced by DNA damage stress in a Rad53-independent manner. We demonstrated that the deletion of NTG1, but not OGG1, resulted in elevated susceptibility to DNA damage agents and oxidative stress inducers. Notably, the ntg1Δ mutant showed growth defects upon antifungal drug treatment. Although deletion of OGG1 or NTG1 did not increase mutation rates, the mutation profile of each ogg1Δ and ntg1Δ mutant was different from that of the wild-type strain. Taken together, we found that DNA N-glycosylase Ntg1 is required for oxidative DNA damage stress and antifungal drug resistance in C. neoformans.

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Fungal DNA damage repair: from model to driver of virulence and AMR
Callum Parkin, Adam G. Bainbridge, Johannes Gregor Matthias Rack
Bioscience Reports.2026;[Epub] CrossRef
Mechanistic Insights into Wildlife Cancer and Conservation Strategies Under the One Health Framework
Qiangqiang Wang, Xiaoxuan Feng, Yurun Su, Naiwen Zhang, Yevheniia Dudnyk, Hongxuan He
Veterinary Sciences.2026; 13(8): 815. CrossRef

Improved tolerance of Escherichia coli to oxidative stress by expressing putative response regulator homologs from Antarctic bacteria: Seo-jeong Park , Sangyong Lim , Jong-il Choi; J. Microbiol. 2020;58(2):131-141. Published online December 23, 2019; DOI: https://doi.org/10.1007/s12275-020-9290-5

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Response regulator (RR) is known a protein that mediates cell’s response to environmental changes. The effect of RR from extremophiles was still under investigation. In this study, response regulator homologs were mined from NGS data of Antarctic bacteria and overexpressed in Escherichia coli. Sixteen amino acid sequences were annotated corresponding to response regulators related to the two-component regulatory systems; of these, 3 amino acid sequences (DRH632, DRH1601 and DRH577) with high homology were selected. These genes were cloned in pRadGro and expressed in E. coli. The transformant strains were subjected to various abiotic stresses including oxidative, osmotic, thermal stress, and acidic stress. There was found that the robustness of E. coli to abiotic stress was increased in the presence of these response regulator homologs. Especially, recombinant E. coli overexpressing drh632 had the highest survival rate in oxidative, hypothermic, osmotic, and acidic conditions. Recombinant E. coli overexpressing drh1601 showed the highest tolerance level to osmotic stress. These results will be applicable for development of recombinant strains with high tolerance to abiotic stress.

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Niallia circulans Q3 enhances microalgal growth in algae-bacteria symbiotic systems for aquaculture wastewater purification
Shenwei Cheng, Yaoting Guan, Junchao Gao, Yanqing Sheng
Bioresource Technology.2026; 459: 135199. CrossRef
Mechanistic and bibliometric insights into RpoS -mediated biofilm regulation and its strategic role in food safety applications
Shirin Akter, Md. Ashikur Rahman, Md. Ashrafudoulla, A.G.M.Sofi Uddin Mahamud, Md Anamul Hasan Chowdhury, Sang-Do Ha
Critical Reviews in Food Science and Nutrition.2025; 65(30): 7070. CrossRef
Adaption strategies of extremophiles and the construction of wastewater treatment systems driven by extremophiles
Zheng Guo, Yong-Guang Li, Zhi-Bin Wang, Xin Zhou, Shou-Qing Ni
Environmental Research.2025; 282: 121979. CrossRef
Deionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli
Seul-Ki Yang, Soyoung Jeong, Inwoo Baek, Jong-il Choi, Sangyong Lim, Jong-Hyun Jung
Microorganisms.2023; 11(9): 2135. CrossRef
Bacterial redox response factors in the management of environmental oxidative stress
Sudharsan M, Rajendra Prasad N, Saravanan Rajendrasozhan
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef
Bacteriophages as Antimicrobial Agents? Proteomic Insights on Three Novel Lytic Bacteriophages Infecting ESBL-Producing Escherichia coli
Sadika Dkhili, Miguel Ribeiro, Salma Ghariani, Houssem Ben Yahia, Mélanie Hillion, Patricia Poeta, Karim Ben Slama, Michel Hébraud, Gilberto Igrejas
OMICS: A Journal of Integrative Biology.2021; 25(10): 626. CrossRef
Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef

Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr: Yong Zhi , Shun Mei Lin , A-Yeung Jang , Ki Bum Ahn , Hyun Jung Ji , Hui-Chen Guo , Sangyong Lim , Ho Seong Seo; J. Microbiol. 2019;57(1):64-73. Published online October 2, 2018; DOI: https://doi.org/10.1007/s12275-019-8416-0

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Abstract PDF

Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Since glucose is crucial for intracellular survival and replication in host cells, we constructed strains with mutations in components of the glucose uptake system, called the phosphotransferase system (PTS), and compared the relative virulence and immune responses in mice. In this study, we found that the strain with mutations in both ptsI and crr (KST0556) was the most attenuated strain among the tested strains, and proved to be highly effective in inducing a mucosal immune response that can protect against NTS infections in mice. Thus, we suggest here that KST0556 (ΔptsIΔcrr) is a potential live vaccine candidate for NTS, and may also be a candidate for a live delivery vector for heterologous antigens. Moreover, since PTS is a well-conserved glucose transporter system in both Gramnegative and Gram-positive bacteria, the ptsI and crr genes may be potential targets for creating live bacterial vectors or vaccine strains.

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Toward the Development of a Live Attenuated Vaccine: Construction and Evaluation of a Salmonella Enteritidis Mutant Strain
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Veterinary Vaccine.2025; 4(4): 100145. CrossRef
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Clinical and Translational Medicine.2025;[Epub] CrossRef
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Experimental Biology and Medicine.2024;[Epub] CrossRef
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Kaining Zhong, Xinting Chen, Junhao Zhang, Xiaoyu Jiang, Junhui Zhang, Minyi Huang, Shuilian Bi, Chunmei Ju, Yongwen Luo
Veterinary Sciences.2024; 11(8): 353. CrossRef
Study of the antibacterial properties of antimicrobial peptide MOp2 from Moringa oleifera seeds against S. aureus through transcriptomic techniques
Zhiyuan Huang, Wenming Dong, Lirong Zou, Qiong Zhao, Yang Tian, Aixiang Huang, Xuefeng Wang
LWT.2024; 191: 115636. CrossRef
EⅡB Mutation Reduces the Pathogenicity of Listeria monocytogenes by Negatively Regulating Biofilm Formation Ability, Infective Capacity, and Virulence Gene Expression
Caixia Liu, Ruixuan Qian, Weidi Shi, Lijun Kou, Jing Wang, Xun Ma, Huijie Ren, Shengjie Gao, Jingjing Ren
Veterinary Sciences.2024; 11(7): 301. CrossRef
Confirmation of Glucose Transporters through Targeted Mutagenesis and Transcriptional Analysis in Clostridium acetobutylicum
Kundi Zhang, Dandan Jiang, Wolfgang Liebl, Maofeng Wang, Lichuan Gu, Ziyong Liu, Armin Ehrenreich
Fermentation.2023; 9(1): 64. CrossRef
Tandem mass tag-based proteomics technology provides insights into multi-targeted mechanism of peptide MOp2 from Moringa oleifera seeds against Staphylococcus aureus
Zhiyuan Huang, Wenming Dong, Jiangping Fan, Yang Tian, Aixiang Huang, Xuefeng Wang
LWT.2023; 178: 114617. CrossRef
A highly-safe live auxotrophic vaccine protecting against disease caused by non-typhoidal Salmonella Typhimurium in mice
Patricia García, Miriam Moscoso, Víctor Fuentes-Valverde, M. Rosario Rodicio, Silvia Herrera-León, Germán Bou
Journal of Microbiology, Immunology and Infection.2023; 56(2): 324. CrossRef
Effect of Antibiotics on the Colonization of Live Attenuated Salmonella Enteritidis Vaccine in Chickens
Jiangang Hu, Chuanyan Che, Jiakun Zuo, Xiangpeng Niu, Zhihao Wang, Liyan Lian, Yuanzheng Jia, Haiyang Zhang, Tao Zhang, Fangheng Yu, Saqib Nawaz, Xiangan Han
Frontiers in Veterinary Science.2021;[Epub] CrossRef
Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens
Wieslaw Swietnicki
Biomolecules.2021; 11(6): 892. CrossRef
Regulator of ribonuclease activity modulates the pathogenicity of Vibrio vulnificus
Jaejin Lee, Eunkyoung Shin, Jaeyeong Park, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(12): 1133. CrossRef
Development of Oxytolerant Salmonella typhimurium Using Radiation Mutation Technology (RMT) for Cancer Therapy
Shuang Gao, Jong-Hyun Jung, Shun-Mei Lin, A-Yeung Jang, Yong Zhi, Ki Bum Ahn, Hyun-Jung Ji, Jae Hyang Lim, Huichen Guo, Hyon E. Choy, Sangyong Lim, Ho Seong Seo
Scientific Reports.2020;[Epub] CrossRef
Transporters of glucose and other carbohydrates in bacteria
Jean-Marc Jeckelmann, Bernhard Erni
Pflügers Archiv - European Journal of Physiology.2020; 472(9): 1129. CrossRef
ptsI gene in the phosphotransfer system is a potential target for developing a live attenuated Salmonella vaccine
Yong Zhi, Shun Lin, Ki Ahn, Hyun Ji, Hui‑Chen Guo, Sangryeol Ryu, Ho Seo, Sangyong Lim
International Journal of Molecular Medicine.2020;[Epub] CrossRef

Microbial radiation-resistance mechanisms: Kwang-Woo Jung , Sangyong Lim , Yong-Sun Bahn; J. Microbiol. 2017;55(7):499-507. Published online June 30, 2017; DOI: https://doi.org/10.1007/s12275-017-7242-5

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Abstract PDF

Organisms living in extreme environments have evolved a wide range of survival strategies by changing biochemical and physiological features depending on their biological niches. Interestingly, organisms exhibiting high radiation resistance have been discovered in the three domains of life (Bacteria, Archaea, and Eukarya), even though a naturally radiationintensive environment has not been found. To counteract the deleterious effects caused by radiation exposure, radiation- resistant organisms employ a series of defensive systems, such as changes in intracellular cation concentration, excellent DNA repair systems, and efficient enzymatic and non-enzymatic antioxidant systems. Here, we overview past and recent findings about radiation-resistance mechanisms in the three domains of life for potential usage of such radiationresistant microbes in the biotechnology industry.

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Oxidative stress response of Deinococcus geothermalis via a cystine importer: Minwook Kim , Sunwook Jeong , Sangyong Lim , Jeonggu Sim , Ho-Gun Rhie , Sung-Jae Lee; J. Microbiol. 2017;55(2):137-146. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6382-y

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A cystine-dependent anti-oxidative stress response is characterized in Deinococcus geothermalis for the first time. Nevertheless, the same transcriptional directed Δdgeo_1985F mutant strain was revealed to have an identical phenotype to the wild-type strain, while the reverse transcriptional directed Δdgeo_1985R mutant strain was more resistant to oxidative stress at a certain concentration of H2O2 than the wild-type strain. The wild-type and mutant strains expressed equal levels of superoxide dismutase and catalase under H2O2-induced stress. Although the expression levels of the general DNAdamage response-related genes recA, pprA, ddrA, and ddrB were up-regulated by more than five-fold in the wild-type strain relative to the Δdgeo_1985R mutant strain, the mutant strain had a higher survival rate than the wild-type under H2O2 stress. The Δdgeo_1985R mutant strain highly expressed a cystine-transporter gene (dgeo_1986), at levels 150-fold higher than the wild-type strain, leading to the conclusion that this cystine transporter might be involved in the defensive response to H2O2 stress. In this study, the cystine transporter was identified and characterized through membrane protein expression analysis, a cystine-binding assay, and assays of intracellular H2O2, cysteine, and thiol levels. The genedisrupted mutant strain of the cystine importer revealed high sensitivity to H2O2 and less absorbed cystine, resulting in low concentrations of total thiol. Thus, the absorbed cystine via this cystine-specific importer may be converted into cysteine, which acts as a primitive defense substrate that non-enzymatically scavenges oxidative stress agents in D. geothermalis.

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The Transposition of Insertion Sequences in Sigma-Factor- and LysR-Deficient Mutants of Deinococcus geothermalis
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Transposition of insertion sequences by dielectric barrier discharge plasma and gamma irradiation in the radiation-resistant bacterium Deinococcus geothermalis
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Proteomic profiling of Deinococcus radiodurans with response to thioredoxin reductase inhibitor and ionizing radiation treatment
Sudharsan M, Rajendra Prasad N, Anindita Chakraborty, Saravanan Rajendrasozhan
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Influence of Redox Imbalances on the Transposition of Insertion Sequences in Deinococcus geothermalis
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Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis
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Transposition of Insertion Sequences was Triggered by Oxidative Stress in Radiation-Resistant Bacterium Deinococcus geothermalis
Chanjae Lee, Nakjun Choi, Min K. Bae, Kyungsil Choo, Sung-Jae Lee
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Deinococcus sedimenti sp. nov. isolated from river sediment: Jae-Jin Lee , Yeon-Hee Lee , Su-Jin Park , Sangyong Lim , Sun-Wook Jeong , Seung-Yeol Lee , Sangkyu Park , Hyo-Won Choi , Myung Kyum Kim , Hee-Young Jung; J. Microbiol. 2016;54(12):802-808. Published online November 26, 2016; DOI: https://doi.org/10.1007/s12275-016-6361-8

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A novel Gram-positive, oval-shaped, non-motile bacterium designated strain 16F1LT was isolated from sediment collected from the Han River in Seoul, Republic of Korea. Based on the 16S rRNA gene sequence (1,448 bp), this strain was identified as a member of the genus Deinococcus that belongs to the class Deinococci. Similarities in the 16S rRNA gene sequence were shown with Deinococcus daejeonensis MJ27T (99.0%), D. grandis DSM 3963T (98.1%), D. radiotolerans C1T (97.5%), and D. caeni Ho-08T (97.2%). Strain 16F1LT was classified as a different genomic species from closely related Deinococcus members, based on less than 70% DNA-DNA relatedness. Genomic DNA G+C content of strain 16F1LT was 67.2 mol%. Strain 16F1LT was found to grow at temperatures of 10–37°C (optimum 25°C) and pH 7–8 (optimum pH 7) on R2A medium, and was catalase-positive and oxidase-negative. Strain 16F1LT showed resistance to gamma radiation (D10 > 2 kGy). In addition, this strain had the following chemotaxonomic characteristics: the major fatty acids were C15:1 ω6c and C16:1 ω7c; the polar lipid profile contained phosphoglycolipids, unknown aminophospholipids, an unknown aminoglycolipid, unknown aminolipids, an unknown glycolipid, an unknown phospholipid, and an unknown polar lipid; the major quinone was MK-8. Phylogenetic, genotypic, phenotypic, and chemotaxonomic characteristics indicated that strain 16F1LT represents a novel species within the genus Deinococcus, for which the name Deinococcus sedimenti sp. nov. is proposed. The type strain is 16F1LT (=KCTC 33796T =JCM 31405T).

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Alejandra Osorio-González, Betsy Anaid Peña-Ocaña, Nancy Abril Martínez-López, José Humberto Castañón-González, Roberto Marín-Paredes, Ricardo Jasso-Chávez, Víctor Manuel Ruíz-Valdiviezo, Vanja Klepac-Ceraj
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Nirjara Singhvi, Chandni Talwar, Shekhar Nagar, Helianthous Verma, Jasvinder Kaur, Nitish Kumar Mahato, Nabeel Ahmad, Krishnendu Mondal, Vipin Gupta, Rup Lal
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Deinococcus fonticola sp. nov., isolated from a radioactive thermal spring in Hungary
Judit Makk, Nóra Tünde Enyedi, Erika Tóth, Dóra Anda, Attila Szabó, Tamás Felföldi, Peter Schumann, Judit Mádl-Szőnyi, Andrea K. Borsodi
International Journal of Systematic and Evolutionary Microbiology.2019; 69(6): 1724. CrossRef
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International Journal of Systematic and Evolutionary Microbiology .2019; 69(11): 3437. CrossRef
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List of new names and new combinations previously effectively, but not validly, published
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Deinococcus rubellus sp. nov., bacteria isolated from the muscle of antarctic fish: Seok-Gwan Choi , Seon Hwa Jeon , Jae-Bong Lee , Eun Sun Joo , Sangyong Lim , Hee-Young Jung , Myung Kyum Kim; J. Microbiol. 2016;54(12):796-801. Published online November 26, 2016; DOI: https://doi.org/10.1007/s12275-016-6390-3

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Two new bacterial strains designated as Ant6T and Ant18 were isolated from the muscle of a fish which had been caught in the Antarctic Ocean. Both strains are Gram-stain-positive, catalase positive, oxidase negative, aerobic, and coccoid bacteria. Phylogenetic analysis based on the 16S rRNA gene sequences of strains Ant6T and Ant18 revealed that the strains Ant6T and Ant18 belong to the genus Deinococcus in the family Deinococcaceae in the class Deinococci. The highest degrees of sequence similarities of strains Ant6T and Ant18 were found with Deinococcus alpinitundrae LMG 24283T by 96.4% and 96.8%, respectively. Strain Ant6T exhibited a high level of DNA- DNA hybridization values with strain Ant18 (82 ± 0.6%). Chemotaxonomic data revealed that the predominant fatty acids were C17􍾙:􍾙0 cyclo, 16:0, and feature 3 (C16:1 ω6c/ω7c) for both strains. A complex polar lipid profile consisted of major amounts of unknown phosphoglycolipids (PGL) and unknown aminophospholipid (APL). Based on the phylogenetic, phenotypic, and chemotaxonomic data, strains Ant6T (=KEMB 9004-169T =JCM 31434T) and Ant18 (=KEMB 9004- 170) should be classified as a new species, for which the name Deinococcus rubellus sp. nov. is proposed.

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Complete genome sequence of Deinococcus rubellus Ant6 isolated from the fish muscle in the Antarctic Ocean
Surajit De Mandal, Sathiyaraj Srinivasan, Junhyun Jeon
Frontiers in Bioengineering and Biotechnology.2023;[Epub] CrossRef

Deinococcus seoulensis sp. nov., a bacterium isolated from sediment at Han River in Seoul, Republic of Korea: Jae-Jin Lee , Yeon-Hee Lee , Su-Jin Park , Sangyong Lim , Sun-Wook Jeong , Seung-Yeol Lee , Young-Je Cho , Myung Kyum Kim , Hee-Young Jung; J. Microbiol. 2016;54(8):537-542. Published online August 2, 2016; DOI: https://doi.org/10.1007/s12275-016-6253-y

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Strain 16F1ET was isolated from a 3-kGy-irradiated sediment sample collected at Han River in Seoul, Republic of Korea. Cells of this strain were observed to be Gram-positive, pililike structure, and short rod shape, and colonies were red in color. The strain showed the highest degree of 16S rRNA gene sequence similarity to Deinococcus aquaticus PB314T (98.8%), Deinococcus depolymerans TDMA-24T (98.1%), Deinococcus caeni Ho-08T (98.0%), and Deinococcus grandis DSM 3963T (97.0%). 16S rRNA gene sequence analysis identified this strain as a member of the genus Deinococcus (Family: Deinococcaceae). The genomic DNA G+C content of strain 16F1ET was 66.9 mol%. The low levels of DNA-DNA hybridization (< 56.2%) with the species mentioned above identified strain 16F1ET as a novel Deinococcus species. Its oxidase and catalase activities as well as the production of acid from glucose were positive. Growth of the strain was observed at 10–37°C (optimum: 20–30°C) and pH 4–10 (optimum: pH 7–8). The cells tolerated less than 5% NaCl and had low resistance to gamma radiation (D10 < 4 kGy). Strain 16F1ET possessed the following chemotaxonomic characteristics: C16:0, C15:1 ω6c, and C16:1 ω7c as the major fatty acids; phosphoglycolipid as the predominant polar lipid; and menaquinone-8 as the predominant respiratory isoprenoid quinone. Based on the polyphasic evidence, as well as the phylogenetic, genotypic, phenotypic, and chemotaxonomic characterization results, strain 16F1ET (=KCTC 33793T =JCM 31404T) is proposed to represent the type strain of a novel species, Deinococcus seoulensis sp. nov.

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Nirjara Singhvi, Chandni Talwar, Shekhar Nagar, Helianthous Verma, Jasvinder Kaur, Nitish Kumar Mahato, Nabeel Ahmad, Krishnendu Mondal, Vipin Gupta, Rup Lal
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Complete Genome Sequence of Deinococcus aquaticus Type Strain PB314, a Non-Extremophile Representative of the Genus Deinococcus
Chad Albert, Jonathan Hill, Leilani Boren, Stacy Scholz-Ng, Nahid Fatema, Ryan Grosso, Erica Soboslay, James Tuohy, David A. Baltrus
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Seokhyeon Bae, Heeyoung Kang, Haneul Kim, Kiseong Joh
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Deinococcus taeanensis sp. nov., a Radiation-Resistant Bacterium Isolated from a Coastal Dune
Ji Hee Lee, Jong-Hyun Jung, Min-Kyu Kim, Sangyong Lim
Current Microbiology.2022;[Epub] CrossRef
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Jana Fahrion, Carina Fink, Paul Zabel, Daniel Schubert, Mohamed Mysara, Rob Van Houdt, Bernhard Eikmanns, Kristina Beblo-Vranesevic, Petra Rettberg
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Deinococcus arcticus sp. nov., isolated from Silene acaulis rhizosphere soil of the Arctic tundra
Xin-Peng Wang, Chang-Ming Li, Yong Yu, Hui-Rong Li, Zong-Jun Du, Da-shuai Mu
International Journal of Systematic and Evolutionary Microbiology .2019; 69(11): 3437. CrossRef
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Qian Wang, Yali Song, Lina Choi, Hongyu Liu, Gejiao Wang, Mingshun Li
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Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, In-Kyu Kang, Leonid N. Ten, Hee-Young Jung
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PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions: Sun-Wook Jeong , Ho Seong Seo , Min-Kyu Kim , Jong-Il Choi , Heon-Man Lim , Sangyong Lim; J. Microbiol. 2016;54(6):426-431. Published online May 27, 2016; DOI: https://doi.org/10.1007/s12275-016-6175-8

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Deinococcus radiodurans is a poly-extremophilic organism, capable of tolerating a wide variety of different stresses, such as gamma/ultraviolet radiation, desiccation, and oxidative stress. PprM, a cold shock protein homolog, is involved in the radiation resistance of D. radiodurans, but its role in the oxidative stress response has not been investigated. In this study, we investigated the effect of pprM mutation on catalase gene expression. pprM disruption decreased the mRNA and protein levels of KatE1, which is the major catalase in D. radiodurans, under normal culture conditions. A pprM mutant strain (pprMMT) exhibited decreased catalase activity, and its resistance to hydrogen peroxide (H2O2) decreased accordingly compared with that of the wild-type strain. We confirmed that RecG helicase negatively regulates katE1 under normal culture conditions. Among katE1 transcriptional regulators, the positive regulator drRRA was not altered in pprM-, while the negative regulators perR, dtxR, and recG were activated more than 2.5-fold in pprMMT. These findings suggest that PprM is necessary for KatE1 production under normal culture conditions by down-regulation of katE1 negative regulators.

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Rising from the fragments: a multi-factorial regulatory framework of DNA damage response in Deinococcus radiodurans
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Development and Regulation of the Extreme Biofilm Formation of Deinococcus radiodurans R1 under Extreme Environmental Conditions
Qiannan Guo, Yuhua Zhan, Wei Zhang, Jin Wang, Yongliang Yan, Wenxiu Wang, Min Lin
International Journal of Molecular Sciences.2023; 25(1): 421. CrossRef
A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
Jordan K. Villa, Runhua Han, Chen-Hsun Tsai, Angela Chen, Philip Sweet, Gabriela Franco, Respina Vaezian, Rok Tkavc, Michael J. Daly, Lydia M. Contreras
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Lack of the Bacterial Phytochrome Protein Decreases Deinococcus radiodurans Resistance to Mitomycin C
Jong-Hyun Jung, Soyoung Jeong, Seonghun Im, Min-Kyu Kim, Ho Seong Seo, Sangyong Lim
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Effects of Conserved Wedge Domain Residues on DNA Binding Activity of Deinococcus radiodurans RecG Helicase
Sun-Wook Jeong, Min-Kyu Kim, Lei Zhao, Seul-Ki Yang, Jong-Hyun Jung, Heon-Man Lim, Sangyong Lim
Frontiers in Genetics.2021;[Epub] CrossRef
The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans
Lihua Gao, Xiaonan Chen, Ye Tian, Yongliang Yan, Yuhua Zhan, Zhengfu Zhou, Wei Zhang, Min Lin, Ming Chen
International Journal of Molecular Sciences.2020; 21(9): 3200. CrossRef
Conservation and diversity of radiation and oxidative stress resistance mechanisms inDeinococcusspecies
Sangyong Lim, Jong-Hyun Jung, Laurence Blanchard, Arjan de Groot
FEMS Microbiology Reviews.2019; 43(1): 19. CrossRef
Gene regulation for the extreme resistance to ionizing radiation of Deinococcus radiodurans
Wuzhou Wang, Yun Ma, Junyan He, Huizhou Qi, Fangzhu Xiao, Shuya He
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PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli
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Knockout of pprM Decreases Resistance to Desiccation and Oxidation in Deinococcus radiodurans
Yang Zeng, Yun Ma, Fangzhu Xiao, Wuzhou Wang, Shuya He
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RNA-Binding Domain is Necessary for PprM Function in Response to the Extreme Environmental Stress in Deinococcus radiodurans
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Spirosoma pulveris sp. nov., a bacterium isolated from a dust sample collected at Chungnam province, South Korea: Eun Sun Joo , Jae-Jin Lee , Seho Cha , Weonhwa Jheong , Taegun Seo , Sangyong Lim , Sun-wook Jeong , Sathiyaraj Srinivasan; J. Microbiol. 2015;53(11):750-755. Published online October 28, 2015; DOI: https://doi.org/10.1007/s12275-015-5263-5

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Strain JSH 5-14T, a Gram-negative, non-motile, and curved rod-shaped bacterium, was isolated from a dust sample collected at Nonsan, Chungnam province, South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain JSH 5-14T revealed that it belongs to the genus Spirosoma, family Cytophagaceae, class Cytophagia. The highest degree of sequence similarities of strain JSH 5-14T were found with Spirosoma liguale DSM 74T (97.8%) and Spirosoma endophyticum EX 36T (96.2%). The predominant fatty acids were summed feature 3 (composed of C16:1 ω7c/C16:1 ω6c) and C16:1ω5c. The major polar lipid was phosphatidylethanolamine, and the predominant respiratory quinone was MK-7. Based on the phylogenetic, chemotaxonomic, and phenotypic data, we propose the strain JSH 5-14T (=KCTC 42550T =JCM 30688T =KEMB 9004-165T) should be classified as a type strain of a novel species, for which the name Spirosoma pulveris sp. nov., is proposed.

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Guillaume Tahon, Liesbeth Lebbe, Anne Willems
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Spirosoma humi sp. nov., Isolated from Soil in South Korea
Li Weilan, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Leonid N. Ten, Hee-Young Jung
Current Microbiology.2018; 75(3): 328. CrossRef
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Leonid N. Ten, Nabil Elderiny, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, Myung Kyum Kim, Hee-Young Jung
Current Microbiology.2018; 75(2): 179. CrossRef
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Weilan Li, Seung-Yeol Lee, In-Kyu Kang, Leonid N. Ten, Hee-Young Jung
Current Microbiology.2018; 75(6): 694. CrossRef
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Weilan Li, Leonid N. Ten, Seung-Yeol Lee, In-Kyu Kang, Hee-Young Jung
International Journal of Systematic and Evolutionary Microbiology.2018; 68(3): 930. CrossRef
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Nabil Elderiny, Leonid N. Ten, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Young-Je Cho, Myung Kyum Kim, Hee-Young Jung
Journal of Microbiology.2017; 55(9): 678. CrossRef
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Joseph Okiria, Leonid N. Ten, Su-Jin Park, Seung-Yeol Lee, Dong Hoon Lee, In-Kyu Kang, Dae Sung Lee, Hee-Young Jung
Journal of Microbiology.2017; 55(12): 927. CrossRef
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Joseph Okiria, Leonid N. Ten, Jae-Jin Lee, Seung-Yeol Lee, Young-Je Cho, Myung Kyum Kim, Hee-Young Jung
International Journal of Systematic and Evolutionary Microbiology.2017; 67(12): 4986. CrossRef
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Journal of Microbiology.2017; 55(11): 850. CrossRef
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Myung Kyum Kim, Ju-Young Kim, Su Jeong Kim, Min Ji Kim, Ju Yeon Lee, Chang-Gyeom Kim, Sathiyaraj Srinivasan
Molecular & Cellular Toxicology.2017; 13(4): 373. CrossRef
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Seon Hwa Jeon, Myung-Suk Kang, Eun Sun Joo, Eun Bit Kim, Sangyong Lim, Sun-wook Jeong, Hee-Young Jung, Sathiyaraj Srinivasan, Myung Kyum Kim
International Journal of Systematic and Evolutionary Microbiology.2016; 66(12): 5077. CrossRef
List of new names and new combinations previously effectively, but not validly, published
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology .2016; 66(11): 4299. CrossRef

Sangyong Lim	0 Article

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