Journal of Microbiology

Sang-Won Lee

3 Articles

Proteomic and Functional Analyses of a Novel Porin-like Protein in Xanthomonas oryzae pv. oryzae: Hye-Jee Park , Sang-Won Lee , Sang-Wook Han; J. Microbiol. 2014;52(12):1030-1035. Published online November 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4442-0

70 View
0 Download
13 Crossref

Proteomic analysis is a useful technique for postulating and elucidating protein functions. In the present work, a shotgun proteomic analysis was used to identify functions of the PXO_03968 gene (previously known as the ax21) from Xanthomonas oryzae pv. oryzae (Xoo), a causal agent for bacterial blight disease in rice. Structural prediction performed on the protein sequence encoded by PXO_03968 reveals that it encodes a putative porin-like protein, possessing a β-barrel domain with 10 β-strands and a signal peptide at the Nterminus. We renamed the gene as an omp1X (outer membrane protein 1 in Xoo), generated its knock out mutant (XooΔomp1X), and compared the protein expression level in the mutant to that in the wild type. A total of 106 proteins displayed more than 1.5-fold difference in expression between the mutant and the wild type strains. COG analysis revealed that these proteins are involved in cell motility as well as signal transduction. In addition, phenotypic analysis demonstrated that motility and biofilm formation in XooΔomp1X are lower than the wild type. These results provide new insights into the functions of outer membrane proteins in Gram-negative bacteria.

Citations

Citations to this article as recorded by

Stenotrophomonas maltophilia virulence: a current view
Vladimir Mikhailovich, Rustam Heydarov, Danila Zimenkov, Igor Chebotar
Frontiers in Microbiology.2024;[Epub] CrossRef
Molecular-genetic portrait of virulence of Stenotrophomonas maltophilia
Vladimir M. Mikhailovich, Rustam N. Geydarov, Julia A. Bocharova, Igor V. Chebotar
Journal of microbiology, epidemiology and immunobiology.2023; 100(5): 380. CrossRef
p‐Aminobenzoic acid inhibits the growth of soybean pathogen Xanthomonas axonopodis pv. glycines by altering outer membrane integrity
Yong‐Hui Jiang, Ting Liu, Xin‐Chi Shi, Daniela D Herrera‐Balandrano, Mei‐Ting Xu, Su‐Yan Wang, Pedro Laborda
Pest Management Science.2023; 79(10): 4083. CrossRef
Expression and function of clpS and clpA in Xanthomonas campestris pv. campestris
Hsueh-Hsia Lo, Hsiao-Ching Chang, Chao-Tsai Liao, Yi-Min Hsiao
Antonie van Leeuwenhoek.2022; 115(5): 589. CrossRef
Outer Membrane Vesicles: Biogenesis, Functions, and Issues
Rokas Juodeikis, Simon R. Carding
Microbiology and Molecular Biology Reviews.2022;[Epub] CrossRef
Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model
Amal Tarek Sarhan, Mohammed Bahey-El-Din, Taha Ibrahim Zaghloul
Vaccine.2021; 39(32): 4471. CrossRef
Deciphering the functions of the outer membrane porin OprBXo involved in virulence, motility, exopolysaccharide production, biofilm formation and stress tolerance in Xanthomonas oryzae pv. oryzae
Nahee Bae, Hye‐Jee Park, Hanbi Park, Minyoung Kim, Sang‐Wook Han
Molecular Plant Pathology.2018; 19(12): 2527. CrossRef
The Ax21 protein influences virulence and biofilm formation in Stenotrophomonas maltophilia
Shi-qi An, Ji-liang Tang
Archives of Microbiology.2018; 200(1): 183. CrossRef
Lsp family proteins regulate antibiotic biosynthesis in Lysobacter enzymogenes OH11
Ruping Wang, Huiyong Xu, Yangyang Zhao, Juan Zhang, Gary Y Yuen, Guoliang Qian, Fengquan Liu
AMB Express.2017;[Epub] CrossRef
Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris
Sarah Schatschneider, Jessica Schneider, Jochen Blom, Fabien Létisse, Karsten Niehaus, Alexander Goesmann, Frank-Jörg Vorhölter
Microbiology.2017; 163(8): 1117. CrossRef
Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
Hye-Jee Park, Sang-Wook Han
The Plant Pathology Journal.2017; 33(6): 602. CrossRef
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
Hye-Jee Park, Chang-Jin Park, Nahee Bae, Sang-Wook Han
The Plant Pathology Journal.2016; 32(3): 266. CrossRef
The effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated Ax21 proteins in Stenotrophomonas maltophilia
Simon Devos, Laurence Van Oudenhove, Stephan Stremersch, Wouter Van Putte, Riet De Rycke, Gonzalez Van Driessche, Jolien Vitse, Koen Raemdonck, Bart Devreese
Frontiers in Microbiology.2015;[Epub] CrossRef

Development of a Chimeric Strain of Porcine Reproductive and Respiratory Syndrome Virus with an Infectious Clone and a Korean Dominant Field Strain: Jung-Ah Lee , Nak-Hyung Lee , Sang-Won Lee , Seung-Yong Park , Chang-Seon Song , In-Soo Choi , Joong-Bok Lee; J. Microbiol. 2014;52(4):345-349. Published online March 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4074-4

85 View
0 Download
18 Crossref

Abstract

The K418 chimeric virus of porcine reproductive and respiratory syndrome virus (PRRSV) was engineered by replacing the genomic region containing structure protein genes of an infectious clone of PRRSV, FL12, with the same region obtained from a Korean dominant field strain, LMY. The K418 reached 106 TCID50/ml of viral titer with similar growth kinetics to those of parental strains and had a cross-reactive neutralizing antibody response to field serum from the entire country. The chimeric clone pK418 can be used as a practical tool for further studying the molecular characteristics of PRRSV proteins through genetic manipulation. Furthermore, successful construction of the K418 will allow for the development of customized vaccine candidates against PRRSV, which has evolved rapidly in Korea.

Citations

Citations to this article as recorded by

Monitoring reported SARS-CoV-2 variants to assess the status of COVID-19 epidemics in the low epidemic state
Bin Liu, Zhongliang Wang, Shanshan Lu, Zhongtian Qi, Zhijie Zhang, Jie Luan, Jianbo Ba
Scientific Reports.2025;[Epub] CrossRef
Bibliometrics and meta -analysis of self-healing bio-concrete – a systematic review
Shahid Ul Islam, Shakeel Ahmad Waseem
European Journal of Environmental and Civil Engineering.2025; 29(5): 933. CrossRef
Porcine Reproductive and Respiratory Syndrome Virus Engineered by Serine Substitution on the 44th Amino Acid of GP5 Resulted in a Potential Vaccine Candidate with the Ability to Produce High Levels of Neutralizing Antibody
Jong-Chul Choi, Min-Sik Kim, Hwi-Yeon Choi, Yeong-Lim Kang, In-Yeong Choi, Sung-Won Jung, Ji-Yun Jeong, Min-Chul Kim, Andrew Y. Cho, Ji-Ho Lee, Dong-Hun Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
Veterinary Sciences.2023; 10(3): 191. CrossRef
Porcine Reproductive and Respiratory Syndrome Virus: Immune Escape and Application of Reverse Genetics in Attenuated Live Vaccine Development
Honglei Wang, Yangyang Xu, Wenhai Feng
Vaccines.2021; 9(5): 480. CrossRef
Evaluation of the Cross-Protective Efficacy of a Chimeric PRRSV Vaccine against Two Genetically Diverse PRRSV2 Field Strains in a Reproductive Model
Chang-Gi Jeong, Amina Khatun, Salik Nazki, Seung-Chai Kim, Yun-Hee Noh, Sang-Chul Kang, Dong-Uk Lee, Myeon-Sik Yang, Nadeem Shabir, In-Joong Yoon, Bumseok Kim, Won-Il Kim
Vaccines.2021; 9(11): 1258. CrossRef
Comparative Evaluation of Antibacterial Activity of Probiotics SK12 and SM18: An In Vitro Study
Shanthala B Mallikarjun, Srihari Nirguna Chandrasekhar, Henna P Salim
International Journal of Clinical Pediatric Dentistry.2021; 13(6): 611. CrossRef
A chimeric porcine reproductive and respiratory syndrome virus (PRRSV)-2 vaccine is safe under international guidelines and effective both in experimental and field conditions
Hwi-Yeon Choi, So-Hyun Lee, So-Hyeun Ahn, Jong-Chul Choi, Ji-Yun Jeong, Beom-Joo Lee, Yeong-Lim Kang, Seong-Soo Hwang, Jung-Keun Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee
Research in Veterinary Science.2021; 135: 143. CrossRef
Effect of encapsulated bacteria on concrete properties: A review
Irfan Yaqoob Wani, Khushpreet Singh
Materials Today: Proceedings.2020; 33: 1706. CrossRef
In vitro and in vivo studies of deglycosylated chimeric porcine reproductive and respiratory syndrome virus as a vaccine candidate and its realistic revenue impact at commercial pig production level
Jung-ju Kim, Jung-Ah Lee, Hwi-yeon Choi, Jang-hyuck Han, Won Huh, Jae-Ho Pi, Jung-Keun Lee, Sangshin Park, Ki-hyun Cho, Joong-bok Lee
Vaccine.2017; 35(37): 4966. CrossRef
Evaluation of the Cross-Protective Efficacy of a Chimeric Porcine Reproductive and Respiratory Syndrome Virus Constructed Based on Two Field Strains
Nadeem Shabir, Amina Khatun, Salik Nazki, Bumseok Kim, Eun-Jin Choi, Dong Sun, Kyoung-Jin Yoon, Won-Il Kim
Viruses.2016; 8(8): 240. CrossRef
Augmented immune responses in pigs immunized with an inactivated porcine reproductive and respiratory syndrome virus containing the deglycosylated glycoprotein 5 under field conditions
Jung-Ah Lee, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Sang-Won Lee
Clinical and Experimental Vaccine Research.2016; 5(1): 70. CrossRef
Influence of biomineralization on a profile of a tropical soil affected by erosive processes
Yamile Valencia González, José Carvalho-Camapum, Luis Augusto Lara-Valencia
DYNA.2015; 82(192): 221. CrossRef
Comparison of the Ability of Two Bacteria to Improve the Behavior of Sandy Soil
Paulo J. Venda Oliveira, Milton S. da Costa, João N. P. Costa, M. Fernanda Nobre
Journal of Materials in Civil Engineering.2015;[Epub] CrossRef
Live porcine reproductive and respiratory syndrome virus vaccines: Current status and future direction
Gourapura J. Renukaradhya, Xiang-Jin Meng, Jay G. Calvert, Michael Roof, Kelly M. Lager
Vaccine.2015; 33(33): 4069. CrossRef
Influence of biomineralization on the physico-mechanical profile of a tropical soil affected by erosive processes
Y. Valencia, J. Camapum, F. Araripe Torres
Soil Biology and Biochemistry.2014; 74: 98. CrossRef
Influences on the carbonate hydrochemistry of mound spring environments, Lake Eyre South region, South Australia
Mark N. Keppel, Vincent E.A. Post, Andrew J. Love, Jonathan D.A. Clarke, Adrian D. Werner
Chemical Geology.2012; 296-297: 50. CrossRef
Mound springs in the arid Lake Eyre South region of South Australia: A new depositional tufa model and its controls
Mark N. Keppel, Jonathan D.A. Clarke, Todd Halihan, Andrew J. Love, Adrian D. Werner
Sedimentary Geology.2011; 240(3-4): 55. CrossRef
Effect of ureolytic bacteria on concrete properties
Rafat Siddique, Navneet Kaur Chahal
Construction and Building Materials.2011; 25(10): 3791. CrossRef

Dysregulation of KSHV Replication by Extracts from Carthamus tinctorius L.: Han Lee , Hyosun Cho , Myoungki Son , Gi-Ho Sung , Taeho Lee , Sang-Won Lee , Yong Woo Jung , Yu Su Shin , Hyojeung Kang; J. Microbiol. 2013;51(4):490-498. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3282-7

56 View
0 Download
2 Scopus

Abstract: Carthamus tinctorius L. (CT) is traditionally used to reduce ailments from diseases of the musculoskeletal system and connective tissue and diseases of blood circulation and the cardiovascular system. Flower extracts from CT are known to have antibacterial activity, anti-inflammatory activity, and to inhibit tumor promotion in mouse skin carcinogenesis. In order to discover new antiviral agents from CT extracts, we tested whether CT extracts contain antiviral activity against gammaherpesvirus infection. This study demonstrated that treatment with CT extracts disrupted KSHV latency in the viral-infected host cells, iSLK-BAC16. n-Hexane and EtOH fractions of CT extracts critically affected at least two stages of the KHSV life-cycle by abnormally inducing KSHV lytic reactivation and by severely preventing KSHV virion release from the viral host cells. In addition to the effects on KSHV itself, CT extract treatments induced cellular modifications by dysregulating cell-cycle and producing strong cytotoxicity. This study demonstrated for the first time that CT extracts have antiviral activities that could be applied to development of new anti-gammaherpesviral agents.

Sang-Won Lee

1 Article

Antiviral effects of heme oxygenase-1 against canine coronavirus and canine influenza virus in vitro: Jae-Hyeong Kim, Dong-Hwi Kim, Kyu-Beom Lim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Dong-Hun Lee, Do-Geun Kim, Hun-Young Yoon, In-Soo Choi; J. Microbiol. 2025;63(5):e2501029. Published online May 27, 2025; DOI: https://doi.org/10.71150/jm.2501029

234 View
14 Download

Abstract PDF Supplementary Material: Heme oxygenase-1 (HO-1) has antioxidant, anti-apoptotic, and anti-inflammatory properties. Emerging evidence shows that HO-1 also exhibits antiviral activity against severe acute respiratory syndrome coronavirus 2, human immunodeficiency virus, hepatitis B virus, and Ebola virus. Its antiviral effects are mediated not only by its enzymatic function but also through the modulation of interferon-related pathways, thereby inhibiting viral replication. In this study, we investigated the antiviral effects of HO-1 on canine coronavirus (CCoV) and canine influenza virus (CIV) H3N2 using cell-based assays. To determine whether HO-1 suppresses CCoV and CIV, cells were treated with hemin to induce HO-1 expression. Hemin treatment successfully induced HO-1 expression in A72 and Madin-Darby canine kidney cells, resulting in the suppression of CCoV and CIV replication. The canine HO-1 gene was cloned into an expression vector and transfected into cells to achieve transient overexpression. Recombinant canine HO-1 protein was expressed in Escherichia coli and purified using an expression vector. HO-1 overexpression suppressed CCoV and CIV replication in cells. Following viral infection, treatment with purified HO-1 protein led to a reduction in viral protein levels. Therefore, both HO-1 expression and exogenous protein treatment effectively inhibited CCoV and CIV replication. Elevated HO-1 protein levels consistently reduced viral RNA and protein expression in vitro. These findings suggest that HO-1 could serve as a potential therapeutic agent for managing viral infections in dogs.

Author index