Journal of Microbiology

Min Zhang

2 Articles

Molecular Analysis of the Copper-Responsive CopRSCD of a Pathogenic Pseudomonas fluorescens Strain: Yong-hua Hu , Hua-lei Wang , Min Zhang , Li Sun; J. Microbiol. 2009;47(3):277-286. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-008-0278-9

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CopRS/CopABCD is one of the known systems that control copper homeostasis in bacteria. Although CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P. fluorescens strain (TSS) isolated from diseased fish. The copRSCD cluster was demonstrated to be required for full copper resistance and regulated at the transcription level by Cu. Expression of copCD is regulated directly by the two-component response regulator CopR, which also regulates its own expression. Interruption of the regulated expression of copR affected bacterial growth, biofilm formation, and tissue dissemination and survival. A mutant CopR, which lacks the N-terminal signal receiver domain and is constitutively active, was found to have an attenuating effect on bacterial virulence when expressed in TSS. To our knowledge, this is the first report that suggests a link between CopR and bacterial pathogenicity in P. fluorescens.

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PLOS Biology.2021; 19(11): e3001446. CrossRef
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Lorena Novoa-Aponte, Cheng Xu, Fernando C. Soncini, José M. Argüello, Craig D. Ellermeier
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HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida
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Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity
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Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis
L Liu, H Chi, L Sun
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Edwardsiella tarda Hfq: impact on host infection and global protein expression
Yong-hua Hu, Yong-xin Li, Li Sun
Veterinary Research.2014;[Epub] CrossRef
An Intimate Link: Two-Component Signal Transduction Systems and Metal Transport Systems in Bacteria
Kamna Singh, Dilani B Senadheera, Dennis G Cvitkovitch
Future Microbiology.2014; 9(11): 1283. CrossRef
Identification and analysis of three virulence-associated TonB-dependent outer membrane receptors of Pseudomonas fluorescens
Sr Zhang, L Zhang, L Sun
Diseases of Aquatic Organisms.2014; 110(3): 181. CrossRef
The impact of transition metals on bacterial plant disease
Helen Fones, Gail M. Preston
FEMS Microbiology Reviews.2013; 37(4): 495. CrossRef
Overlapping and unique contributions of two conserved polysaccharide loci in governing distinct survival phenotypes in Vibrio vulnificus
Yunzhi Guo, Dean A. Rowe‐Magnus
Environmental Microbiology.2011; 13(11): 2888. CrossRef
Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain
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Vaccine.2010; 28(40): 6603. CrossRef

Cys-92, Cys-95, and the C-Terminal 12 Residues of the Vibrio harveyi Ferric Uptake Regulator (Fur) are Functionally Inessential: Kun Sun , Shuang Cheng , Min Zhang , Fang Wang , Li Sun; J. Microbiol. 2008;46(6):670-680. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0113-3

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Abstract

Ferric uptake regulator (Fur) is a global regulator involved in multiple aspects of bacterial life. The gene encoding the Vibrio harveyi Fur (FurVh) was cloned from a pathogenic V. harveyi strain isolated from diseased fish. FurVh shares 77% overall sequence identity with the Escherichia coli Fur (FurEc) and could complement a mutant of FurEc. Like FurEc, FurVh possesses two cysteine residues at positions 92 and 95, yet unlike FurEc, in which these cysteine residues constitute part of the metal ion coordination site and hence are vital to the repressor activity, C92 and C95 of FurVh proved to be functionally inessential. Further study identified a Vibrio Fur signature sequence, which is preserved in all the ten Vibrio Fur proteins that have been discovered to date but in none of the non-vibrio Fur proteins. Site-directed and random mutation analyses of the signature residues, the cysteine residues, and seven highly charged amino acid residues indicated that D9, H32, C137, and K138 of FurVh are functionally important but D9, C137, and K138 can be replaced by more than one functional substitutes. Systematic deletion analysis demonstrated that the C-terminal 12 residues of FurVh are functionally inessential. These results (i) indicated that the activation mechanism, or certain aspects of which, of FurVh is possibly different from that of FurEc; and (ii) suggested that it is not very likely that the C-terminal 12 residues play any significant role in the activation or stability of FurVh; and (iii) provided insights into the potential function of the local structure involving C137 and K138.

Citations

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Comparative analysis of the survival and gene expression of pathogenic strainsVibrio harveyiafter starvation
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The FUR (ferric uptake regulator) superfamily: Diversity and versatility of key transcriptional regulators
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Archives of Biochemistry and Biophysics.2014; 546: 41. CrossRef
DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda
Kun Sun, Xu-dong Jiao, Min Zhang, Li Sun
Veterinary Microbiology.2010; 141(1-2): 149. CrossRef
Experimental and computational characterization of the ferric uptake regulator from Aliivibrio salmonicida (Vibrio salmonicida)
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Crystal structure of the Vibrio cholerae ferric uptake regulator (Fur) reveals insights into metal co‐ordination
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Attenuation ofEdwardsiella tardaVirulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing
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Domain analysis of the Edwardsiella tarda ferric uptake regulator
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Min Zhang

1 Article

Different Adaption Strategies of Abundant and Rare Microbial Communities in Sediment and Water of East Dongting Lake: Yabing Gu, Junsheng Li, Zhenghua Liu, Min Zhang, Zhaoyue Yang, Huaqun Yin, Liyuan Chai, Delong Meng, Nengwen Xiao; J. Microbiol. 2024;62(10):829-843. Published online October 22, 2024; DOI: https://doi.org/10.1007/s12275-024-00171-8

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Abstract: The dynamics of aquatic microbes is of great importance for comprehending the acclimatisation and evolution of microorganisms in lake ecology. However, little is known about the adaption strategies of microbial communities in East Dongting Lake, which had special and complexity geographical characteristics. A semi-enclosed lake area (A) and a waterway connected to Yangtze River (B) both existed in the lake zone. Here, we investigated bacterial and fungal community diversity, community network and community assembly processes in sediment and water. The results indicated that the proportion of OTU numbers and their relative abundance for rare and abundant taxa were different obviously between sediment and water, but not between bacteria and fungi. However, abundant subcommunities dominated the shifts of bacterial community diversity and structure in A region, while rare subcommunities for fungal community diversity. Compared to fungal community, bacterial network was more compact and more key stones were identified as rare taxa. In addition, stochastic processes (dispersal limitation) drove the community assembly of abundant and rare subcommunities, but the effects of deterministic processes (including variable and heterogeneous selections) affected more on rare rather than abundant taxa. Partial Mantel test further indicated that the effect of environmental factors was a stronger force in shaping abundant bacterial subcommunities (TOC, NH₄⁺-N, TN, and ORP) and rare fungal subcommunities (ORP). Environmental factors explained more of the variation in bacterial community structure than that in fungal community structure, although they had additional effects on fungal community diversity and community assembly. Moreover, bacterial community affected the fungal community as a biotic factor in water. This research provided new insights into better understanding of microbial communities in the complex environment of the East Dongting Lake.

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