- Prevalence of Indigenous Antibiotic‑Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra‑Broad Specificity
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Jaein Choe , Su-Hyeon Kim , Ji Min Han , Jong-Hoon Kim , Mi-Sun Kwak , Do-Won Jeong , Mi-Kyung Park
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J. Microbiol. 2023;61(12):1063-1073. Published online January 2, 2024
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DOI: https://doi.org/10.1007/s12275-023-00098-6
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 Abstract
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The consumption of fresh produce has led to increase in antibiotic-resistant (AR) Salmonella outbreaks. In this study, indigenous
Salmonella was isolated from a total of two hundred-two samples including fresh produce and agricultural environmental
samples in Korea. After biochemical confirmation using the Indole, Methyl Red, Voges-Proskauer, Citrate tests, presumable
Salmonella isolates were identified by 16S rRNA sequencing. Identified Salmonella isolates were evaluated for antibiotic
susceptibility against twenty-two antibiotics. The specificity and the efficiency of plating (EOP) of vB_SalS_KFSSM were
evaluated against fifty-three bacterial strains. Twenty-five suspected Salmonella were isolated and confirmed by the positive
result
for methyl red and citrate, of which ten were identified as Salmonella spp. through 16S rRNA gene sequencing. Eight
Salmonella isolates (4.0%, n = 8/202) were resistant to at least one antibiotic, among which five were multi-drug resistant. As
a lytic phage against Salmonella spp. CMGS-1, vB_SalS_KFSSM was isolated from cow manure. The phage was observed as
a tailed phage belonging to the class Caudoviricetes. It exhibited an intra-broad specificity against four indigenous AR Salmonella
isolates, two indigenous Salmonella isolates, and five other Salmonella serotypes with great efficiencies (EOP ≥ 0.75).
Thus, this study suggested the potential of vB_SalS_KFSSM to combat indigenous AR Salmonella.
- Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection
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In Young Choi , Cheonghoon Lee , Won Keun Song , Sung Jae Jang , Mi-Kyung Park
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J. Microbiol. 2019;57(2):170-179. Published online January 31, 2019
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DOI: https://doi.org/10.1007/s12275-019-8610-0
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Abstract
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Since Salmonella Enteritidis is one of the major foodborne
pathogens, on-site applicable rapid detection methods have
been required for its control. The purpose of this study was
to isolate and purify S. Enteritidis-specific phage (KFS-SE2
phage) from an eel farm and to investigate its feasibility as a
novel, efficient, and reliable bio-receptor for its employment.
KFS-SE2 phage was successfully isolated at a high concentration
of (2.31 ± 0.43) × 1011 PFU/ml, and consisted of an
icosahedral head of 65.44 ± 10.08 nm with a non-contractile
tail of 135.21 ± 12.41 nm. The morphological and phylogenetic
analysis confirmed that it belongs to the Pis4avirus genus
in the family of Siphoviridae. KFS-SE2 genome consisted
of 48,608 bp with 45.7% of GC content. Genome analysis
represented KFS-SE2 to have distinctive characteristics as a
novel phage. Comparative analysis of KFS-SE2 phage with
closely related strains confirmed its novelty by the presence
of unique proteins. KFS-SE2 phage exhibited excellent specificity
to S. Enteritidis and was stable under the temperature
range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time
was determined to be 20 min. Overall, a new lytic KFS-SE2
phage was successfully isolated from the environment at a
high concentration and the excellent feasibility of KFS-SE2
phage was demonstrated as a new bio-receptor for S. Enteritidis
detection.
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Citations
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