- Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study
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Patricia Giovanella , Lídia de Azevedo Duarte , Daniela Mayumi Kita , Valéria Maia de Oliveira , Lara Durães Sette
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J. Microbiol. 2021;59(7):634-643. Published online May 15, 2021
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DOI: https://doi.org/10.1007/s12275-021-0395-2
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Soil contamination with diesel oil is quite common during
processes of transport and storage. Bioremediation is considered
a safe, economical, and environmentally friendly approach
for contaminated soil treatment. In this context, studies
using hydrocarbon bioremediation have focused on total
petroleum hydrocarbon (TPH) analysis to assess process effectiveness,
while ecotoxicity has been neglected. Thus, this
study aimed to select a microbial consortium capable of detoxifying
diesel oil and apply this consortium to the bioremediation
of soil contaminated with this environmental pollutant
through different bioremediation approaches. Gas chromatography
(GC-FID) was used to analyze diesel oil degradation,
while ecotoxicological bioassays with the bioindicators
Artemia sp., Aliivibrio fischeri (Microtox), and Cucumis
sativus were used to assess detoxification. After 90 days of
bioremediation, we found that the biostimulation and biostimulation/
bioaugmentation approaches showed higher rates
of diesel oil degradation in relation to natural attenuation
(41.9 and 26.7%, respectively). Phytotoxicity increased in the
biostimulation and biostimulation/bioaugmentation treatments
during the degradation process, whereas in the Microtox
test, the toxicity was the same in these treatments as that
in the natural attenuation treatment. In both the phytotoxicity
and Microtox tests, bioaugmentation treatment showed lower
toxicity. However, compared with natural attenuation, this
approach did not show satisfactory hydrocarbon degradation.
Based on the microcosm experiments results, we conclude
that a broader analysis of the success of bioremediation requires
the performance of toxicity bioassays.
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Citations
Citations to this article as recorded by  - Strategy of nitrate-enhanced natural attenuation for remediation of PAHs-contaminated subsoil
Xuyang Jiang, Zhen Mao, Zhenqi Hu, Tao Jin, Licun Zhong, Jinbiao Yu
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- Production, Partial Characterization, and Immobilization in Alginate Beads of an Alkaline Protease from a New Thermophilic Fungus Myceliophthora sp.
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Letícia Maria Zanphorlin , Fernanda Dell Antonio Facchini , Filipe Vasconcelos , Rafaella Costa Bonugli-Santos , André Rodrigues , Lara Durães Sette , Eleni Gomes , Gustavo Orlando Bonilla-Rodriguez
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DOI: https://doi.org/10.1007/s12275-010-9269-8
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793
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Thermophilic fungi produce thermostable enzymes which have a number of applications, mainly in biotechnological processes. In this work, we describe the characterization of a protease produced in solidstate (SSF) and submerged (SmF) fermentations by a newly isolated thermophilic fungus identified as a putative new species in the genus Myceliophthora. Enzyme-production rate was evaluated for both fermentation processes, and in SSF, using a medium composed of a mixture of wheat bran and casein, the proteolytic output was 4.5-fold larger than that obtained in SmF. Additionally, the peak of proteolytic activity was obtained after 3 days for SSF whereas for SmF it was after 4 days. The crude enzyme obtained by both
SSF and SmF displayed similar optimum temperature at 50°C, but the optimum pH shifted from 7 (SmF) to 9 (SSF). The alkaline protease produced through solid-state fermentation (SSF), was immobilized on beads of calcium alginate, allowing comparative analyses of free and immobilized proteases to be carried out. It was
observed that both optimum temperature and thermal stability of the immobilized enzyme were higher than for the free enzyme. Moreover, the immobilized enzyme showed considerable stability for up to 7 reuses.
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Citations
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