Journal of Microbiology

Lae-Hyung Kang

3 Articles

Genetic Analysis of the Capsid Region of Norovirus GII.4 Variants Isolated in South Korea: Ju-Eun Kim , Sung-Geun Lee , Han-Gil Cho , Sang-Ha Han , Lae-Hyung Kang , Youn-Mi Lee , Chul-Jong Park , Soon-Young Paik; J. Microbiol. 2014;52(5):427-434. Published online April 11, 2014; DOI: https://doi.org/10.1007/s12275-014-3538-x

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Norovirus is one of the major causes of non-bacterial gas-troenteritis in humans. The aim of this study was to analyze the amino acid variation of open reading frame 2 of GII.4 variants in South Korea during the period from November 2006 to December 2012. Sixty-nine complete nucleotide se-quences of open reading frame 2 were obtained from 113 GII.4 strains. The GII.4 2006b variants were detected pre-dominantly between 2006 and 2009; however, new GII.4 variants, which were termed the 2010 variant and the 2012 variant, emerged in 2010 and 2012, respectively. The num-ber of GII.4 2006b variants steadily decreased until 2012, whereas the number of gastroenteritis cases caused by the new variants increased between 2010 and 2012. The amino acid sequence in the ORF2 region obtained in this study was compared with other GII.4 variants isolated in various countries. Amino acid variations were observed primarily at epitope sites and the surrounding regions. Amino acids 294, 359, 393, and 413 of the P2 subdomain were the most variable sites among the GII.4 variants. The information in this study can be useful in basic research to predict the emergence and determine the genetic functions of new GII.4 variants.

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Determining the efficacy of 27 commercially available disinfectants against human noroviruses
Jae-Woong Lee, Lae-Hyung Kang, Min-Kyeong Kim, Jeong-Soon Kim, Myung L. Kim, Sung-Geun Lee, In-Hye Choi, Chul-Jong Park, Soon-Young Paik
Journal of Infection and Public Health.2021; 14(2): 244. CrossRef
Evolutionary changes in the capsid P2 region of Australian strains of the norovirus GII.Pe_GII.4
Leesa D. Bruggink, Jean M. Moselen, Jason A. Roberts, John A. Marshall
Journal of Medical Microbiology.2017; 66(7): 1014. CrossRef
A norovirus intervariant GII.4 recombinant in Victoria, Australia, June 2016: the next epidemic variant?
Leesa Bruggink, Michael Catton, John Marshall
Eurosurveillance.2016;[Epub] CrossRef
Complete Nucleotide Sequence Analysis of the Norovirus GII.4 Sydney Variant in South Korea
Ji-Sun Park, Sung-Geun Lee, Ji-Young Jin, Han-Gil Cho, Weon-Hwa Jheong, Soon-Young Paik
BioMed Research International.2015; 2015: 1. CrossRef
Molecular epidemiology of norovirus GII.4 variants in children under 5 years with sporadic acute gastroenteritis in South Korea during 2006–2013
Han-Gil Cho, Sung-Geun Lee, Ju-Eun Kim, Kyeong-Sin Yu, Deog-Yong Lee, Po-Hyun Park, Mi-hye Yoon, Eek-Hoon Jho, Jaehong Kim, Soon-Young Paik
Journal of Clinical Virology.2014; 61(3): 340. CrossRef

Simultaneous Detection of Waterborne Viruses by Multiplex Real-Time PCR: Lae-Hyung Kang , Se-hwan Oh , Jeong-Woong Park , Yu-Jung Won , Sangryeol Ryu , Soon-Young Paik; J. Microbiol. 2013;51(5):671-675. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-3199-1

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Norovirus, Rotavirus group A, the Hepatitis A virus, and Coxsackievirus are all common causes of gastroenteritis. Conventional diagnoses of these causative agents are based on antigen detection and electron microscopy. To improve the diagnostic potential for viral gastroenteritis, internally controlled multiplex real-time polymerase chain reaction (PCR) methods have been recently developed. In this study, individual real-time PCRs were developed and optimized for specific detections of Norovirus genogroup I, Norovirus genogroup II, Rotavirus group A, the Hepatitis A virus, and Coxsackievirus group B1. Subsequently, individual PCRs were combined with multiplex PCR reactions. In general, multiplex real-time PCR assays showed comparable sensitivities and specificities with individual assays. A retrospective clinical evaluation showed increased pathogen detection in 29% of samples using conventional PCR methods. Prospective clinical evaluations were detected in 123 of the 227 (54%) total samples used in the multiplex realtime PCR analysis. The Norovirus genogroup II was found most frequently (23%), followed by Rotavirus (20%), the Hepatitis A virus (4.5%), Coxsackievirus (3.5%), and Norovirus genogroup I (2.6%). Internally controlled multiplex real-time PCR assays for the simultaneous detection of Rotavirus, Coxsackievirus group B, the Hepatitis A virus, and Norovirus genogroups I and II showed significant improvement in the diagnosis of viral gastroenteritis.

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Investigation of the presence of human adenovirus, norovirus, and rotavirus in tap water in Marrakech, Morocco
Nouhaila Elfellaki, Salma Berrouch, Houda Rafi, Simeon Goïta, Hibatallah Lachkar, Jamal Eddine Hafid, Gemma Reguera
Applied and Environmental Microbiology.2026;[Epub] CrossRef
Microbiological quality of irrigation water for cultivation of fruits and vegetables: An overview of available guidelines, water testing strategies and some factors that influence compliance.
Oluwadara Alegbeleye, Anderson S. Sant’Ana
Environmental Research.2023; 220: 114771. CrossRef
Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality
Lisa Paruch
International Journal of Environmental Research and Public Health.2022; 19(9): 5128. CrossRef
Development and Evaluation of a SYBR Green-Based, Real-time Polymerase Chain Reaction for Rapid and Specific Detection of Human Coxsackievirus B5
Kyu Bong Cho
Biomedical Science Letters.2020; 26(4): 302. CrossRef
Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review
Mohammad Irshad, Priyanka Gupta, Dhananjay Singh Mankotia, Mohammad Ahmad Ansari
World Journal of Gastroenterology.2016; 22(20): 4824. CrossRef
Quantification and Genotyping of Rotavirus A within Two Wastewater Treatment Processes
Chourouk Ibrahim, Nadia Cherif, Salah Hammami, Pierre Pothier, Abdennaceur Hassen
CLEAN – Soil, Air, Water.2016; 44(4): 393. CrossRef
Microfluidic Quantitative PCR for Simultaneous Quantification of Multiple Viruses in Environmental Water Samples
Satoshi Ishii, Gaku Kitamura, Takahiro Segawa, Ayano Kobayashi, Takayuki Miura, Daisuke Sano, Satoshi Okabe, C. A. Elkins
Applied and Environmental Microbiology.2014; 80(24): 7505. CrossRef

Full Sequence Analysis and Characterization of a Human Astrovirus Type 1 Isolate from South Korea: Sung-Geun Lee , Lae-Hyung Kang , Weon-Hwa Jheong , Mi-Hwa Oh , Gyu-Cheol Lee , Sujeong Park , Soon-Young Paik; J. Microbiol. 2013;51(1):123-129. Published online March 2, 2013; DOI: https://doi.org/10.1007/s12275-013-2505-2

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Human astroviruses are recognized as an important cause of infantile gastroenteritis around the world. In South Korea, sporadic cases of HAstV infection have been reported since 2002. However, hitherto, there have been no studies reporting the whole genome sequence of an HAstV isolate from South Korea. Hence, we sequenced and analyzed the entire genome of an HAstV-1 strain (lhar) that was isolated in Seoul, South Korea. The whole-genome sequence analysis revealed 3 open reading frames comprising the whole genome: ORF1a (2,763 bp), ORF1b (1,548 bp), and ORF2 (2,364 bp). The lhar strain showed amino acid identities with 8 other reference strains of 87.6–98.7%, 94.2–98.8%, and 62.6–99.0% in the ORF1a, ORF1b, and ORF2 regions, respectively. The amino acid sequence of the capsid region encoded by ORF2 was compared with a total of 19 HAstV-1 strains and 8 HAstVs reference strains isolated in various countries. This revealed 1 amino acid substitution, at aa412 (Pro → Arg) in ORF2. This study, the first to report the fulllength sequence of an HAstV isolated in South Korea, is meaningful in that it can be used as a full-length HAstV sequence standard for future comparison studies. It may also prove useful to the field of public health field by facilitating the diagnosis and the prediction of new emerging variants.

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Full Sequence Analysis and Characterization of Human Bocavirus Type 2 in South Korea
Bomina Paik, Sung-Geun Lee , Han-Gil Cho, Yu-Jung Won, Lae-Hyung Kang, Soon-Young Paik, Seung-Jin Hong
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Complete genome sequencing of a recombinant strain between human astrovirus antigen types 2 and 8 isolated from South Korea
Hyun-Ju Ha, Sung-Geun Lee, Han-Gil Cho, Ji-Young Jin, Jae Woong Lee, Soon-Yong Paik
Infection, Genetics and Evolution.2016; 39: 127. CrossRef

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