- Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene
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Ju-Yong Jeong , Kyung-Ik Gil , Kyong-Hee Lee , Jong-Ok Ka
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J. Microbiol. 2008;46(6):599-607. Published online December 24, 2008
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DOI: https://doi.org/10.1007/s12275-008-0174-3
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Specific fecal DNA markers were investigated for major pollution sources, cow, human, and pig, and occurrence of the identified markers was analyzed in river waters using Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniques and sequencing of 16S rDNA of Bacteroides-Prevotella. The unique and specific DNA markers for cow and human were identified as a 222 bp and 60 bp peak in HaeIII T-RFLP profiles, respectively, and the pig-specific marker was not identified but the unique T-RFLP profile of pig could be used as a substitution. Human-specific marker was detected in most of the river waters tested (92.1%) and T-RFLP profiles of river waters were shown to be similar to those of human feces. Cluster analysis of T-RFLP data showed that the fecal sources were multiple (human plus cow and human plus dairy cow) in most of the river waters. The phylogenetic analysis for the clones recovered from the fecal and water samples showed that the clones from cow formed a discreet cluster from those of other sources. The other clones from human, pig, and river water formed two groups all together. The results of this study could be used to identify and control the fecal pollution source in the bodies of water in Korea.
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Citations
Citations to this article as recorded by  - Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing
Ju-Yong Jeong, Hee-Deung Park, Kyong-Hee Lee, Hang-Yeon Weon, Jong-Ok Ka
The Journal of Microbiology.2011; 49(4): 585. CrossRef - Application of an Integrated Community Analysis Approach for Microbial Source Tracking in a Coastal Creek
Yiping Cao, Laurie C. Van De Werfhorst, Bram Sercu, Jill L. S. Murray, Patricia A. Holden
Environmental Science & Technology.2011; 45(17): 7195. CrossRef
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