- Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature
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Chanhyeok Park , Kyeong Eui Yun , Jeong Min Chu , Ji Yeon Lee , Chang Pyo Hong , Young Do Nam , Jinuk Jeong , Kyudong Han , Yong Ju Ahn
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J. Microbiol. 2020;58(8):703-710. Published online June 25, 2020
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DOI: https://doi.org/10.1007/s12275-020-0092-6
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The gut microbiome, which is symbiotic within the human
body, assists in human digestion. It plays significant roles
in identifying intestinal disease as well as in maintaining a
healthy body with functional immune and metabolic activities.
To confirm the consistency of fecal intestinal microbial
research, it is necessary to study the changes in intestinal microbial
flora according to the fecal collection solution and
storage period. We collected fecal samples from three healthy
Korean adults. To examine the efficacy of fecal collection solution,
we used NBgene-Gut, OMNIgene-Gut, 70% ethanol
(Ethanol-70%), and RNAlater. The samples were stored for
up to two months at room temperature using three different
methods
, and we observed changes in microbial communities
over time. We analyzed clusters of changes in the microbial
flora by observing fecal stock solutions and metagenome
sequencing performed over time. In particular, we confirmed
the profiling of alpha and beta diversity and microbial classification
according to the differences in intestinal environment
among individuals. We also confirmed that the microbial
profile remained stable for two months and that the microbial
profile did not change significantly over time. In addition,
our results suggest the possibility of verifying microbial
profiling even for long-term storage of a single sample. In conclusion,
collecting fecal samples using a stock solution rather
than freezing feces seems to be relatively reproducible and
stable for GUT metagenome analysis. Therefore, stock solution
tubes in intestinal microbial research can be used without
problems.
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Citations
Citations to this article as recorded by  - Five-Day Treatment with B. licheniformis Along with Classical Vancomycin Treatment Was Effective in Preserving Gut Microbiota in Patients with Clostridioides difficile Infection
Tae-Geun Gweon, Sang-Bum Kang, Soo-Young Na, Dong Jun Oh, Sang Wook Kim, Geom Seog Seo, Joo Young Cho
Nutrients.2025; 17(4): 641. CrossRef - Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing results
Christopher E. Stamper, Andrew J. Hoisington, Joseph C. Ellis, Christopher A. Lowry, Lisa A. Brenner, Theodore Raymond Muth
PLOS One.2025; 20(8): e0324351. CrossRef - Stability of oral and fecal microbiome at room temperature: impact on diversity
Blanca Rius-Sansalvador, David Bars-Cortina, Olfat Khannous-Lleiffe, Ainhoa Garcia-Serrano, Elisabet Guinó, Ester Saus, Toni Gabaldón, Victor Moreno, Mireia Obón-Santacana
Frontiers in Microbiomes.2025;[Epub] CrossRef - Omnigene-Guttm ensures fecal microbiome stability in the pediatric population
Raoull Hoogendijk, Thijs J. M. van den Broek, Hyunju Lee, Sabine Mueller, Cassie Kline, John Bianco, Janetta Top, Marcel R. de Zoete, Lennart Kester, Friso Calkoen, Jasper van der Lugt
AMB Express.2024;[Epub] CrossRef - Microbes for lung cancer detection: feasibility and limitations
Sirui Zhou, Weijian Zhu, Hehua Guo, Yalan Nie, Jiazheng Sun, Ping Liu, Yulan Zeng
Frontiers in Oncology.2024;[Epub] CrossRef - Importance of Human Faecal Biobanking: From Collection to Storage
Izabela Lewandowska, Katarzyna Grzech, Jolanta Krzysztoń-Russjan
Advancements of Microbiology.2024; 63(4): 181. CrossRef - Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Ting Cai, Lun-xi Liang, Fen Wang, Po-Yu Liu
Microbiology Spectrum.2023;[Epub] CrossRef - Microbial Composition and Stool Short Chain Fatty Acid Levels in Fibromyalgia
Yunkyung Kim, Geun-Tae Kim, Jihun Kang
International Journal of Environmental Research and Public Health.2023; 20(4): 3183. CrossRef - Best practice for wildlife gut microbiome research: A comprehensive review of methodology for 16S rRNA gene investigations
Leigh Combrink, Ian R. Humphreys, Quinn Washburn, Holly K. Arnold, Keaton Stagaman, Kristin D. Kasschau, Anna E. Jolles, Brianna R. Beechler, Thomas J. Sharpton
Frontiers in Microbiology.2023;[Epub] CrossRef - Effects of Waterlogging on Soybean Rhizosphere Bacterial Community Using V4, LoopSeq, and PacBio 16S rRNA Sequence
Taobing Yu, Lang Cheng, Qi Liu, Shasha Wang, Yuan Zhou, Hongbin Zhong, Meifang Tang, Hai Nian, Tengxiang Lian, Cheng Gao, Glade Dlott
Microbiology Spectrum.2022;[Epub] CrossRef - Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis
Xin-meng Li, Xiao Shi, Yao Yao, Yi-cun Shen, Xiang-ling Wu, Fen Wang
SSRN Electronic Journal .2022;[Epub] CrossRef - Trialling a microbiome-targeted dietary intervention in children with ADHD—the rationale and a non-randomised feasibility study
Kate Lawrence, Kyriaki Myrissa, Miguel Toribio-Mateas, Lori Minini, Alice M. Gregory
Pilot and Feasibility Studies.2022;[Epub] CrossRef - Can the genetic variability of Blastocystis sp. be associated with the climatic region of its human carriers?
B. Ake-Canche, E. Rodriguez-Bataz, J.Y Esquivel-Piña, A. Tolentino-Loreto, S. Arroyo-Escalante, J. Martínez-Ocaña, M. Romero-Valdovinos, O. Valenzuela, G.E. Orozco-Mosqueda, F. Martinez-Hernandez, P. Maravilla, A. Martinez
Infection, Genetics and Evolution.2022; 106: 105383. CrossRef - Critical evaluation of faecal microbiome preservation using metagenomic analysis
Alena L Pribyl, Donovan H Parks, Nicola Z Angel, Joel A Boyd, Alexander G Hasson, Liang Fang, Samantha L MacDonald, Blake A Wills, David L A Wood, Lutz Krause, Gene W Tyson, Philip Hugenholtz
ISME Communications.2021;[Epub] CrossRef - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
Jinuk Jeong, Kyeongeui Yun, Seyoung Mun, Won-Hyong Chung, Song-Yi Choi, Young-do Nam, Mi Young Lim, Chang Pyo Hong, ChanHyeok Park, Yong Ju Ahn, Kyudong Han
Scientific Reports.2021;[Epub] CrossRef - Dietary fiber and probiotics influence the gut microbiome and melanoma immunotherapy response
Christine N. Spencer, Jennifer L. McQuade, Vancheswaran Gopalakrishnan, John A. McCulloch, Marie Vetizou, Alexandria P. Cogdill, Md A. Wadud Khan, Xiaotao Zhang, Michael G. White, Christine B. Peterson, Matthew C. Wong, Golnaz Morad, Theresa Rodgers, Jona
Science.2021; 374(6575): 1632. CrossRef - Impact of Plant-Based Meat Alternatives on the Gut Microbiota of Consumers: A Real-World Study
Miguel A. Toribio-Mateas, Adri Bester, Natalia Klimenko
Foods.2021; 10(9): 2040. CrossRef
- Arenimonas aquaticum sp. nov., a Member of the Gammaproteobacterium, Isolated from a Freshwater Reservoir
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A-Ram Kim , Siwon Lee , Kyudong Han , Tae-Young Ahn
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J. Microbiol. 2013;51(6):887-887.
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DOI: https://doi.org/10.1007/s12275-013-0728-x
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In the article by Kim et al. that appears in Journal of Microbiology 2012; 50, 354-358. The novel species name Arenimonas
aquaticum should be changed as Arenimonas aquatica because the genus name Arenimonas is of the feminine gender. The
adjective used as specific epithet must be in the feminine gender as well. In addition, type strain NA-09T =KACC14663T should
be changed as type strain NA-09T =KACC14662T.
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Citations
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María Rosa Pino-Otín, Guillermo Lorca, Elisa Langa, Francisco Roig, Eva M. Terrado, Diego Ballestero
International Journal of Molecular Sciences.2023; 24(23): 16960. CrossRef
- Arenimonas aquaticum sp. nov., a Member of the Gammaproteobacterium, Isolated from a Freshwater Reservoir
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A-Ram Kim , Siwon Lee , Kyudong Han , Tae-Young Ahn
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J. Microbiol. 2012;50(2):354-358. Published online April 27, 2012
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DOI: https://doi.org/10.1007/s12275-012-1301-8
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675
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6
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Abstract
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A novel bacterial strain, designated NA-09T, was isolated
from a freshwater sample collected from the Cheonho reservoir,
Republic of Korea. Colonies were creamy-white pigmented,
translucent, and circular with convex shape. The
isolate was Gram-staining negative, strictly aerobic, motile,
and rod-shaped. The 16S rRNA gene sequence analysis revealed
that strain NA-09T belonged to the genus Arenimonas
and showed the highest sequence similarities with Arenimonas
malthae CC-JY-1T (95.4%), A. oryziterrae YC6267T
(94.9%), A. composti P2-12-1T (94.8%), and A. donghaensis
H03-R19T (94.1%). The major fatty acids were iso-C16:0
(20.8%), iso-C15:0 (16.9%), summed feature 1 (13.2%), and
iso-C16:1ω7c alcohol (10.2%). The major isoprenoid quinone
of the isolate was ubiquionone-8. On the basis of the data
from the polyphasic characterization, the strain NA-09T
represents a novel species, for which the name Arenimonas
aquaticum sp. nov. is proposed (type strain NA-09T =KACC
14663T =NBRC 106550T).
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Citations
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Kseniya S. Zayulina, Maria I. Prokofeva, Alexander G. Elcheninov, Margarita P. Voytova, Andrei A. Novikov, Tatiana V. Kochetkova, Ilya V. Kublanov
International Journal of Systematic and Evolutionary Microbiology.2020; 70(4): 2726. CrossRef -
Arenimonas terrae sp. nov., isolated from orchard soil
Dong Min Han, Byung Hee Chun, Hyung Min Kim, Shehzad Abid Khan, Che Ok Jeon
International Journal of Systematic and Evolutionary Microbiology
.2020; 70(1): 537. CrossRef - Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample
Jie Zhu, Hui-Min Wang, Qi Zhang, Wei-Wei Dong, De-Long Kong, Yan-Wei Wang, Jin-Long Song, Xu Jiang, Shu-Miao Zhao, Wei Zhang, Zhi-Yong Ruan
Antonie van Leeuwenhoek.2017; 110(8): 1027. CrossRef - Nitrate removal efficiency of a mixotrophic denitrification wall for nitrate-polluted groundwater in situ remediation
Rui Li, Chuanping Feng, Beidou Xi, Nan Chen, Yu Jiang, Ying Zhao, Mingxiao Li, Qiuling Dang, Baowei Zhao
Ecological Engineering.2017; 106: 523. CrossRef -
Arenimonas soli sp. nov., isolated from saline–alkaline soil
Lian Xu, Ji-Quan Sun, Xia Liu, Xiao-Zhen Liu, Ming-Quan Qiao, Xiao-Lei Wu
International Journal of Systematic and Evolutionary Microbiology
.2017; 67(8): 2829. CrossRef - On the way to identify microorganisms in drinking water distribution networks via DNA analysis of the gut content of freshwater isopods
Michael Mayer, Adrian Keller, Ulrich Szewzyk, Hans-Joachim Warnecke
Journal of Biotechnology.2015; 201: 54. CrossRef
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