Journal of Microbiology

Kyoung-Hee Oh

4 Articles

Assessing the microcystins concentration through optimized protein phosphatase inhibition assay in environmental samples: Kyoung-Hee Oh , Kung-Min Beak , Yuna Shin , Young-Cheol Cho; J. Microbiol. 2022;60(6):602-609. Published online April 30, 2022; DOI: https://doi.org/10.1007/s12275-022-2020-4

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Protein phosphatase (PPase) inhibition assay (PPIA) is widely used to analyze the concentration of microcystins (MCs) because it is comparatively less expensive and faster than other assays. This study aimed to optimize the PPIA by determining a suitable reaction terminator and an optimal methanol concentration in the sample. The most suitable reaction time was 90 min, with the corresponding methanol concentration in the sample being 15% or less. When p-nitrophenyl phosphate (pNPP) was used as a substrate, copper chloride solution was suitably used as a reaction terminator, and when 4- methylumbelliferyl phosphate (MUP) was used, a glycine buffer not only increased the measurement sensitivity of the reaction product but also terminated the enzymatic reaction. When PPase 1 and MUP were used as an enzyme and a substrate, respectively, the limit of quantitation for MC-leucine/ arginine (LR) was 0.02 μg/L, whereas it was 0.1 μg/L when pNPP was used as a substrate. The proposed method facilitated the measurement of MC-LR concentration without additional pretreatments, such as concentration or purification; therefore, this method was suitable and feasible for the continuous monitoring of MCs in drinking water.

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Magnetic solid phase extraction coupled with high-performance liquid chromatography-diode array detection based on assembled magnetic covalent organic frameworks for selective extraction and detection of microcystins in aquatic foods
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Journal of Chromatography A.2022; 1685: 463614. CrossRef

Quantification of Toxigenic Microcystis spp. in Freshwaters by Quantitative Real-time PCR Based on the Microcystin Synthetase A Gene: Kyoung-Hee Oh , Dong-Hwan Jeong , Young-Cheol Cho; J. Microbiol. 2013;51(1):18-24. Published online March 2, 2013; DOI: https://doi.org/10.1007/s12275-013-2354-z

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Abstract PDF

A method to estimate the abundance of toxigenic Microcystis in environmental samples by using quantitative real-time PCR was developed and optimized. The basis of this method is the amplification of a highly conserved region of the mcyA gene within the microcystin synthetase gene cluster. Using this method, the average copy number of mcyA gene per cell in toxigenic Microcystis strains was estimated. The molecular markers and method developed in this study can be used to monitor toxigenic strains of Microcystis in Korean freshwaters, in which harmful cyanobacterial blooms are routinely found.

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Field-Based Mesocosm Evidence for the Role of Natural Nutrient Sources in Cyanobacterial Blooms
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Environmental Analysis Health and Toxicology.2026; 41(1): e2026005. CrossRef
Application of Iron-Modified Activated Carbon for Phosphate Removal in Aqueous Systems
Won-Hyeong Seo, Ga-Eun Kim, Ji-Yoon Lee, Soohoon Choi
Applied Sciences.2025; 15(10): 5353. CrossRef
eDNA- and eRNA-Based Detection of 2-Methylisoborneol-Producing Cyanobacteria and Intracellular Synthesis Dynamics in Freshwater Ecosystem
Keonhee Kim, Chaehong Park, Nan-Young Kim, Soon-Jin Hwnag
Biology.2025; 14(10): 1377. CrossRef
Prediction of Cyanotoxin Episodes in Freshwater: A Case Study on Microcystin and Saxitoxin in the Lobo Reservoir, São Paulo State, Brazil
Munique de Almeida Bispo Moraes, Raphaella de Abreu Magalhães Rodrigues, Raju Podduturi, Niels Ole Gerslev Jørgensen, Maria do Carmo Calijuri
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Effective Early Treatment of Microcystis Exponential Growth and Microcystin Production with Hydrogen Peroxide and Hydroxyapatite
Ian Struewing, Nathan Sienkiewicz, Chiqian Zhang, Nicholas Dugan, Jingrang Lu
Toxins.2022; 15(1): 3. CrossRef
Assessing the microcystins concentration through optimized protein phosphatase inhibition assay in environmental samples
Kyoung-Hee Oh, Kung-Min Beak, Yuna Shin, Young-Cheol Cho
Journal of Microbiology.2022; 60(6): 602. CrossRef
Microcystins Concentration in Fishes Collected from the Weirs of Four Rivers in Korea and Risk Assessment
Do-Hwan Kim, Yuna Shin, Min Jeong Park, Young-Cheol Cho
Korean Journal of Ecology and Environment.2022; 55(2): 120. CrossRef
Toxic cyanobacteria and microcystin dynamics in a tropical reservoir: assessing the influence of environmental variables
Thanh-Luu Pham, Thi Hoang Yen Tran, Kazuya Shimizu, Qintong Li, Motoo Utsumi
Environmental Science and Pollution Research.2021; 28(45): 63544. CrossRef
Assessment of different mcy genes for detecting the toxic to non-toxic Microcystis ratio in the field by multiplex qPCR
Jun Zuo, Liting Chen, Kun Shan, Lili Hu, Lirong Song, Nanqin Gan
Journal of Oceanology and Limnology.2018; 36(4): 1132. CrossRef
Detection of microcystin-producing cyanobacteria in water samples using loop-mediated isothermal amplification targeting mcyB gene
Mohandass Ramya, Muthukrishnan Kayalvizhi, Gopalakrishnan Haripriya, Pasupathi Rathinasabapathi
3 Biotech.2018;[Epub] CrossRef
Molecular and ecological characterization of toxic cyanobacteria from the Bay of Quinte (Lake Ontario) and Maumee Bay (Lake Erie)
Kirsten M. Müller, Aline Chhun, Stephanie J. Guildford, Sarah J. Yakobowski, Miroslava Jonlija
Journal of Great Lakes Research.2017; 43(6): 1067. CrossRef
Dynamic variation of toxic and non-toxic Microcystis proportion in the eutrophic Daechung Reservoir in Korea
Seung-Hyun Joung, Hee-Mock Oh, Kyung-A You
Journal of Microbiology.2016; 54(8): 543. CrossRef
Recruitment of cyanobacteria from the sediments in the eutrophic Shanzi Reservoir
Yuping Su, Xuejing You, Hui Lin, Huiru Zhuang, Yuan Weng, Dayi Zhang
Environmental Technology.2016; 37(6): 641. CrossRef
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Ana Pacheco, Iame Guedes, Sandra Azevedo
Toxins.2016; 8(6): 172. CrossRef
Autumnal increase in proportion of toxic Microcystis in Lake Taihu depends more on temperature adaptability than on effects of toxigenicity
L. Hu, R. Kong, X. Xu
Journal of Applied Microbiology.2015; 119(3): 744. CrossRef
Evaluation of Contamination Level of the Sediments from Chusori and Chudong Areas in Daechung Reservoir
Kyoung-Hee Oh, Young-Cheol Cho
Journal of Korean Society of Environmental Engineers.2015; 37(5): 277. CrossRef

Molecular Characterization of Polychlorinated Biphenyl-Dechlorinating Populations in Contaminated Sediments: Kyoung-Hee Oh , Ellen B. Ostrofsky , Young-Cheol Cho; J. Microbiol. 2008;46(2):165-173. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-007-0214-4

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Abstract PDF

Polychlorinated biphenyl (PCB)-dechlorinating microorganisms were characterized in PCB-contaminated sediments using amplified ribosomal DNA restriction analysis (ARDRA). The sediments were prepared by spiking Aroclor 1248 into PCB-free sediments, and were inoculated with microorganisms eluted from St. Lawrence River sediments. PCB-free sediments inoculated with the same inoculum served as the control. Four restriction fragment length polymorphism (RFLP) groups in the eubacterial and two in the archaeal domain were found exclusively in PCB-spiked sediment clone libraries. Sequence analysis of the four eubacterial clones showed homology to Escherichia coli, Lactosphaera pasteurii, Clostridium thermocellum, and Dehalobacter restrictus. The predominant archaeal sequence in the PCB-spiked sediment clone library was closely related to Methanosarcina barkeri, which appear to support earlier findings that methanogens are involved in PCB dechlorination. When the dot-blot hybridization was performed between the sediment DNA extract and the probes designed with eubacterial RFLP groups, the intensity of two of eubacterial RFLP groups, which showed high sequence homology to C. pascui and D. restrictus, was highly correlated with the number of dechlorinating microorganisms suggesting these two members intend to contribute to PCB dechlorination.

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Biodegradation of polyvinyl chloride plastic films by enriched anaerobic marine consortia
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Marine Environmental Research.2020; 158: 104949. CrossRef
Microbial Characterization of Methanogenic and Iron-reducing Consortium in Reactors with Polychlorinated Biphenyls
Mara R. de Lima e Silva, Regiane C. Correa, Isabel K. Sakamoto, Maria B. A. Varesche
Current Microbiology.2018; 75(6): 666. CrossRef
Indirect Evidence Link PCB Dehalogenation with Geobacteraceae in Anaerobic Sediment-Free Microcosms
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A Chloroflexi bacterium dechlorinates polychlorinated biphenyls in marine sediments under in situ-like biogeochemical conditions
Giulio Zanaroli, Annalisa Balloi, Andrea Negroni, Luigimaria Borruso, Daniele Daffonchio, Fabio Fava
Journal of Hazardous Materials.2012; 209-210: 449. CrossRef
Biotransformation of a highly chlorinated PCB mixture in an activated sludge collected from a Membrane Biological Reactor (MBR) subjected to anaerobic digestion
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Journal of Hazardous Materials.2011; 186(2-3): 2060. CrossRef
Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs)
Giulio Zanaroli, Annalisa Balloi, Andrea Negroni, Daniele Daffonchio, Lily Y. Young, Fabio Fava
Journal of Hazardous Materials.2010; 178(1-3): 417. CrossRef
Characterization of a Dehalobacter Coculture That Dechlorinates 1,2-Dichloroethane to Ethene and Identification of the Putative Reductive Dehalogenase Gene
Ariel Grostern, Elizabeth A. Edwards
Applied and Environmental Microbiology.2009; 75(9): 2684. CrossRef

Effects of Sulfate Concentration on the Anaerobic Dechlorination of Polychlorinated Biphenyls in Estuarine Sediments: Young-Cheol Cho , Kyoung-Hee Oh; J. Microbiol. 2005;43(2):166-171.; DOI: https://doi.org/2167 [pii]

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Abstract PDF: In order to determine the effects of sulfate concentration on the anaerobic dechlorination of polychlorinated biphenyls, sediments spiked with Aroclor 1242 were made into slurries using media which had various sulfate concentrations ranging from 3 to 23 mM. The time course of dechlorination clearly demonstrated that dechlorination was inhibited at high concentration of sulfate due to less dechlorination of meta-substituted congeners. When the dechlorination patterns were analyzed by the calculation of Euclidean distance, the dechlorination pathway in the 3 mM sulfate samples was found to be different from that observed in the 13 mM samples, although the extent of dechlorination in these two samples was similar. It is possible that the dechlorination in the high sulfate concentration samples is inhibited by the suppression of growth of methanogen, which have been shown to be meta-dechlorinating microorganisms.

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