Journal of Microbiology

Kye-Heon Oh

5 Articles

Comparative Analysis of 2,4,6-Trinitrotoluene (TNT)-Induced Cellular Responses and Proteomes in Pseudomonas sp. HK-6 in Two Types of Media: Yun-Seok Cho , Bheong-Uk Lee , Hyung-Yeel Kahng , Kye-Heon Oh; J. Microbiol. 2009;47(2):220-224. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0108-0

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TNT-induced cellular responses and proteomes in Pseudomonas sp. HK-6 were comparatively analyzed in two different media: basal salts (BS) and Luria broth (LB). HK-6 cells could not degrade more than 0.5 mM TNT with BS medium, while in LB medium, they exhibited the enhanced capability to degrade as much as 3.0 mM TNT. Analysis of total cellular fatty acids in HK-6 cells suggested that the relative abundance of several saturated or unsaturated fatty acids is altered under TNT-mediated stress conditions. Scanning electron microscopy showed the presence of perforations, irregular rod formations, and wrinkled extracellular surfaces in cells under TNT stress. Proteomic analysis of soluble protein fractions from HK-6 <br>cultures grown with TNT as a substrate revealed 11 protein spots induced by TNT. Among these, seven proteins (including Alg8, AlgB, NirB, and the AhpC/Tsa family) were detected only in LB medium containing TNT. The proteins AspS, Tsf, and assimilatory nitrate reductase were increasingly expressed only in BS medium containing TNT. The protein dGTPase was found to be induced and expressed when cells were grown in either type of TNT-containing media. These results provide a better understanding of the cytotoxicity and survival mechanism used by Pseudomonas sp. HK-6 when placed under TNT stress conditions.

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New insights into xenobiotic tolerance of Antarctic bacteria: transcriptomic analysis of Pseudomonas sp. TNT3 during 2,4,6-trinitrotoluene biotransformation
Ma. Ángeles Cabrera, Sebastián L. Márquez, José M. Pérez-Donoso
Environmental Science and Pollution Research.2024; 31(11): 17256. CrossRef
Bacillus pumilus proteome changes in response to 2,4,6-trinitrotoluene-induced stress
Galina Yakovleva, William Kurdy, Anna Gorbunova, Irina Khilyas, Guenter Lochnit, Olga Ilinskaya
Biodegradation.2022; 33(6): 593. CrossRef
Improvement of Thermotolerance of Zymomonas mobilis by Genes for Reactive Oxygen Species-Scavenging Enzymes and Heat Shock Proteins
Sakunda Anggarini, Masayuki Murata, Keisuke Kido, Tomoyuki Kosaka, Kaewta Sootsuwan, Pornthap Thanonkeo, Mamoru Yamada
Frontiers in Microbiology.2020;[Epub] CrossRef
The Sycamore Maple Bacterial Culture Collection From a TNT Polluted Site Shows Novel Plant-Growth Promoting and Explosives Degrading Bacteria
Sofie Thijs, Wouter Sillen, Sascha Truyens, Bram Beckers, Jonathan van Hamme, Pieter van Dillewijn, Pieter Samyn, Robert Carleer, Nele Weyens, Jaco Vangronsveld
Frontiers in Plant Science.2018;[Epub] CrossRef
Species-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity in human, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene
Ekaterina Anatolevna Naumenko, Barbara Ahlemeyer, Eveline Baumgart-Vogt
Environmental Toxicology.2017; 32(3): 989. CrossRef
Proteomic Analysis of 2,4,6-Trinitrotoluene Degrading Yeast Yarrowia lipolytica
Irina V. Khilyas, Guenter Lochnit, Olga N. Ilinskaya
Frontiers in Microbiology.2017;[Epub] CrossRef
Enhancement of microbial 2,4,6-trinitrotoluene transformation with increased toxicity by exogenous nutrient amendment
Shih-Hsiung Liang, Duen-Wei Hsu, Chia-Ying Lin, Chih-Ming Kao, Da-Ji Huang, Chih-Ching Chien, Ssu-Ching Chen, Isheng Jason Tsai, Chien-Cheng Chen
Ecotoxicology and Environmental Safety.2017; 138: 39. CrossRef
Microbial degradation of expired slurry explosives in mines: a review
Anuradha, Biswajit Paul, Jagdish
International Journal of Environmental Studies.2015; 72(1): 117. CrossRef
A toxicological review on potential microbial degradation intermediates of 2,4,6-trinitrotoluene, and its implications in bioremediation
Muhammad Imran Khan, Jaejin Lee, Joonhong Park
KSCE Journal of Civil Engineering.2013; 17(6): 1223. CrossRef
Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain
Bheong-Uk Lee, Moon-Seop Choi, Kye-Heon Oh
Biotechnology and Bioprocess Engineering.2013; 18(6): 1224. CrossRef
Biodegradation and biotransformation of explosives
Elizabeth L Rylott, Astrid Lorenz, Neil C Bruce
Current Opinion in Biotechnology.2011; 22(3): 434. CrossRef

Construction of an Escherichia-Pseudomonas Shuttle Vector Containing an Aminoglycoside Phosphotransferase Gene and a lacZ'''' Gene for α-Complementation: Bheong-Uk Lee , Ja-Heon Hong , Hyung-Yeel Kahng , Kye-Heon Oh; J. Microbiol. 2006;44(6):671-673.; DOI: https://doi.org/2458 [pii]

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Abstract PDF: A new 4.87 kb Escherichia-Pseudomonas shuttle vector has been constructed by inserting a 1.27 kb DNA fragment with a replication origin of a Pseudomonas plasmid pRO1614 into the 3.6 kb E. coli plasmid pBGS18. This vector, designated pJH1, contains an aminoglycoside phosphotransferase gene (aph) from Tn903, a lacZ'''' gene for α-complementation and a versatile multiple cloning site possessing unique restriction sites for EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, and HindIII. When pJH1 was transformed into E. coli DH5α and into P. putida HK-6, it was episomally and stably maintained in both strains. In addition, the enhanced green fluorescent protein (EGFP) gene which was transcriptionally cloned into pJH1 rendered E. coli cells fluorescence when its transformants were illuminated at 488 nm.

Molecular Detection of Catabolic Genes for Polycyclic Aromatic Hydrocarbons in the Reed Rhizosphere of Sunchon Bay: Hyung-Yeel Kahng , Kye-Heon Oh; J. Microbiol. 2005;43(6):572-576.; DOI: https://doi.org/2287 [pii]

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Abstract PDF: This study focused on detecting catabolic genes for polycyclic aromatic hydrocarbons (PAHs) distributed in the reed rhizosphere of Sunchon Bay, Korea. These marsh and mud environments were severely affected by human activities, including agriculture and fisheries. Our previous study on microbial roles in natural decontamination displayed the possibility that PAH-degrading bacteria, such as Achromobacter sp., Alcaligenes sp., Burkholderia sp. and Pseudomonas sp. play an important decontamination role in a reed rhizosphere. In order to gain further fundamental knowledge on the natural decontamination process, catabolic genes for PAH metabolism were investigated through PCR amplification of dioxygenase genes using soil genomic DNA and sequencing. Comparative analysis of predicted amino acid sequences from 50 randomly selected dioxygenase clones capable of hydroxylating inactivated aromatic nuclei indicated that these were divided into three groups, two of which might be originated from PAH-degrading bacteria. Amino acid sequences of each dioxygenase clone were a part of the genes encoding enzymes for initial catabolism of naphthalene, phenanthrene, or pyrene that might be originated from bacteria in the reed rhizosphere of Sunchon Bay.

Introduction of Saxicolous Lichens Distributed in Coastal Rocks of U-do Islet in Jeju, Korea: Hyung-Yeel Kahng , Byoung-Jun Yoon , Sung-Hyun Kim , Duck-Ja Shin , Jae-Seoun Hur , Hyun-Woo Kim , Eui-Sung Kang , Kye-Heon Oh , Young Jin Koh; J. Microbiol. 2004;42(4):292-298.; DOI: https://doi.org/2108 [pii]

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Abstract PDF: This study reports, for the first time, the ivestigation of the distribution of Korean saxicolous lichens in the coastal rocks of U-do islet, which is known as an unpolluted zone in Jeju. More than thirty lichens were obtained and investigated from the coastal rocks frequently contacted by seawater. A molecular analysis using PCR amplification of the rRNA ITS regions revealed the coastal rock lichens could be placed into 8 families and 14 genera, Ramalinaceae (Bacidia, Ramalina), Physciaceae (Buellia, Dirinaria, Phaeophyscia, Physcia, Pyxine), Lecanoraceae (Candelaria, Lecanora), Parmeliaceae (Xanthoparmelia), Graphidaceae (Graphis), Pertusariaceae (Pertusaria), Rhizocarpaceae (Rhizocarpon), and Teloschistaceae (Caloplaca), showing a diversity of lichens, with foliose (flat leaf-like), crustose (crust-like), and fruticose (miniature shrub-like) life forms might be distributed in the coastal rocks. These findings suggested the possibility that the lichens identified in the present work might be resistant to a salty environment.

Construction of a Bioluminescent Reporter Using the luc Gene and meta-Cleavage Dioxygenase Promoter for Detection of Catecholic Compounds: Sang-Ho Park , Dong-Hun Lee , Kye-Heon Oh , Chi-Kyung Kim; J. Microbiol. 2000;38(3):183-186.

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Abstract PDF: Several types of bioluminescent reporter strains have been developed for the detection and monitoring of pollutant aromatics contaminating the environment. In this study, a bioluminescent reporter strain, E. coli SHP3, was constructed by fusing the luc gene of firefly luciferase with the promoter of pcbC responsible for the meta-cleavage of aromatic hydrocarbons. The bioluminescence expressed by the luc gene in the reporter was well triggered by the promoter when it was exposed to 2,3-dihydroxybiphenyl (2,3-DHBP) at 0.5 to 1 mM concentrations. The bioluminescent response was more extensive when the reporter strain was exposed to 5 mM catechol and 2 mM 4-chlorocatechol. These different types of bioluminescent responses by E. coli SHP3 appeared to be characterized by the nature of the aromatics to stress. Since E. coli SHP3 responded to 2,3-DHBP quite sensitively, this reporter strain could be applied for detecting some catecholic pollutants.

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